scholarly journals Preparation of Komagataeibacter xylinus inoculum for bacterial cellulose biosynthesis using magnetically assisted external-loop airlift bioreactor

2021 ◽  
Author(s):  
Anna Żywicka ◽  
Daria Ciecholewska-Juśko ◽  
Radosław Drozd ◽  
Rafał Rakoczy ◽  
Maciej Konopacki ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Crystallinity Index ◽  
Dry Mass ◽  
Airlift Bioreactor ◽  
Cellular Density ◽  
External Loop ◽  
Inoculum Age ◽  
Komagataeibacter Xylinus ◽  
Inoculum Preparation ◽  
Magnetically Assisted

The aim of this study was to demonstrate the applicability of a novel magnetically-assisted external-loop airlift bioreactor (EL-ALB), equipped with RMF generators for the preparation of Komagataeibacter xylinus inoculum during three-cycle repeated fed-batch cultures, further used for bacterial cellulose (BC) production. The fermentation carried out in the RMF-assisted EL-ALB allowed to obtain an inoculum of more than 200x higher cellular density compared to classical methods of inoculum preparation. The inoculum obtained in the RMF-assisted EL-ALB was characterized by a high and stable metabolic activity during repeated batch fermentation process. The application of the RMF-assisted EL-ALB for K. xylinus inoculum production did not induce the formation of cellulose-deficient mutants. It was also confirmed that the ability of K. xylinus to produce BC was at the same level (7.26 g/L of dry mass), regardless of inoculum age. Additionally, the BC obtained from the inoculum produced in the RMF-assisted EL-ALB was characterized by reproducible mechanical strength, nanostructure and total crystallinity index. The results obtained in this study may find multiple applications in any biotechnological processes requiring a high-quality bacterial inoculum.

scholarly journals Preparation of Komagataeibacter xylinus Inoculum for Bacterial Cellulose Biosynthesis Using Magnetically Assisted External-Loop Airlift Bioreactor

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3950
Author(s):  
Anna Żywicka ◽  
Daria Ciecholewska-Juśko ◽  
Radosław Drozd ◽  
Rafał Rakoczy ◽  
Maciej Konopacki ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Negative Impact ◽  
Crystallinity Index ◽  
Rotating Magnetic Field ◽  
Airlift Bioreactor ◽  
Fibrillar Structure ◽  
External Loop ◽  
Komagataeibacter Xylinus ◽  
Inoculum Preparation ◽  
Magnetically Assisted

The aim of this study was to demonstrate the applicability of a novel magnetically assisted external-loop airlift bioreactor (EL-ALB), equipped with rotating magnetic field (RMF) generators for the preparation of Komagataeibacter xylinus inoculum during three-cycle repeated fed-batch cultures, further used for bacterial cellulose (BC) production. The fermentation carried out in the RMF-assisted EL-ALB allowed to obtain an inoculum of more than 200 × higher cellular density compared to classical methods of inoculum preparation. The inoculum obtained in the RMF-assisted EL-ALB was characterized by a high and stable metabolic activity during repeated batch fermentation process. The application of the RMF-assisted EL-ALB for K. xylinus inoculum production did not induce the formation of cellulose-deficient mutants. It was also confirmed that the ability of K. xylinus to produce BC was at the same level (7.26 g/L of dry mass), regardless of inoculum age. Additionally, the BC obtained from the inoculum produced in the RMF-assisted EL-ALB was characterized by reproducible water-related properties, mechanical strength, nano-fibrillar structure and total crystallinity index. The lack of any negative impact of inoculum preparation method using RMF-assisted EL-ALB on BC properties is of paramount value for its future applications, including use as a biomaterial in tissue engineering, wound healing, and drug delivery, where especially BC liquid capacity, nanostructure, crystallinity, and mechanical properties play essential roles.

scholarly journals Bacterial Cellulose (BC) and BC Composites: Production and Properties

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 192
Author(s):  
Tatiana G. Volova ◽  
Svetlana V. Prudnikova ◽  
Evgeniy G. Kiselev ◽  
Ivan V. Nemtsev ◽  
Alexander D. Vasiliev ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Synthesis Process ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Surface Culture ◽  
E Coli ◽  
Productive Process ◽  
Komagataeibacter Xylinus ◽  
Submerged Conditions ◽  
Properties Of Composites

The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m2 and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.

Tailoring bacterial cellulose structure through CRISPR interference‐mediated downregulation of galU in Komagataeibacter xylinus CGMCC 2955

2020 ◽  
Vol 117 (7) ◽  
pp. 2165-2176 ◽  
Author(s):  
Long‐Hui Huang ◽  
Qi‐Jing Liu ◽  
Xue‐Wen Sun ◽  
Xue‐Jing Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Structure ◽  
Crispr Interference ◽  
Komagataeibacter Xylinus

scholarly journals Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Ahmed Alemam ◽  
Tharwat Shaheen ◽  
Saad El-Din Hassan ◽  
Said Desouky ◽  
Mamdouh El-Gamal
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Nanofiber ◽  
Komagataeibacter Xylinus ◽  
Static Conditions

scholarly journals Encapsulation of Activated Carbon into a Hollow-Type Spherical Bacterial Cellulose Gel and Its Indole-Adsorption Ability Aimed at Kidney Failure Treatment

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1076
Author(s):  
Toru Hoshi ◽  
Masahito Endo ◽  
Aya Hirai ◽  
Masashige Suzuki ◽  
Takao Aoyagi
Keyword(s):  
Activated Carbon ◽  
Cell Suspension ◽  
Bacterial Cellulose ◽  
Silicone Oil ◽  
Adsorption Ability ◽  
Alginate Gel ◽  
Initial Stage ◽  
Intra Particle Diffusion ◽  
Pass Through ◽  
Komagataeibacter Xylinus

For reducing side effects and improvement of swallowing, we studied the encapsulation of activated carbon formulations with a hollow-type spherical bacterial cellulose (HSBC) gel using two kinds of encapsulating methods: Methods A and B. In Method A, the BC gelatinous membrane was biosynthesized using Komagataeibacter xylinus (K. xylinus) at the interface between the silicone oil and cell suspension containing activated carbon. In Method B, the bacterial cellulose (BC) gelatinous membrane was formed at the interface between the cell suspension attached to the alginate gel containing activated carbon and the silicone oil. After the BC gelatinous membrane was biosynthesized by K. xylnus, alginate gel was removed by soaking in a phosphate buffer. The activated carbon encapsulated these methods could neither pass through the BC gelatinous membrane of the HSBC gel nor leak from the interior cavity of the HSBC gel. The adsorption ability was evaluated using indole, which is a precursor of the uremic causative agent. From curve-fitting, the adsorption process followed the pseudo-first-order and intra-particle diffusion models, and the diffusion of the indole molecules at the surface of the encapsulated activated carbon within the HSBC gel was dominant at the initial stage of adsorption. It was observed that the adsorption of the encapsulated activated carbon by the intraparticle diffusion process became dominant with longer adsorption times.

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