Tailoring bacterial cellulose structure through CRISPR interference‐mediated downregulation of galU in Komagataeibacter xylinus CGMCC 2955

2020 ◽  
Vol 117 (7) ◽  
pp. 2165-2176 ◽  
Author(s):  
Long‐Hui Huang ◽  
Qi‐Jing Liu ◽  
Xue‐Wen Sun ◽  
Xue‐Jing Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Structure ◽  
Crispr Interference ◽  
Komagataeibacter Xylinus

scholarly journals Bacterial Cellulose (BC) and BC Composites: Production and Properties

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 192
Author(s):  
Tatiana G. Volova ◽  
Svetlana V. Prudnikova ◽  
Evgeniy G. Kiselev ◽  
Ivan V. Nemtsev ◽  
Alexander D. Vasiliev ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Synthesis Process ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Surface Culture ◽  
E Coli ◽  
Productive Process ◽  
Komagataeibacter Xylinus ◽  
Submerged Conditions ◽  
Properties Of Composites

The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m2 and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.

scholarly journals Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Ahmed Alemam ◽  
Tharwat Shaheen ◽  
Saad El-Din Hassan ◽  
Said Desouky ◽  
Mamdouh El-Gamal
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Nanofiber ◽  
Komagataeibacter Xylinus ◽  
Static Conditions

Engineering quaternized chitosan in the 3D bacterial cellulose structure for antibacterial wound dressings

2020 ◽  
Vol 86 ◽  
pp. 106490 ◽  
Author(s):  
Haiyong Ao ◽  
Wenwen Jiang ◽  
Yanjiao Nie ◽  
Chen Zhou ◽  
Jiajia Zong ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressings ◽  
Cellulose Structure ◽  
Quaternized Chitosan

scholarly journals Encapsulation of Activated Carbon into a Hollow-Type Spherical Bacterial Cellulose Gel and Its Indole-Adsorption Ability Aimed at Kidney Failure Treatment

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1076
Author(s):  
Toru Hoshi ◽  
Masahito Endo ◽  
Aya Hirai ◽  
Masashige Suzuki ◽  
Takao Aoyagi
Keyword(s):  
Activated Carbon ◽  
Cell Suspension ◽  
Bacterial Cellulose ◽  
Silicone Oil ◽  
Adsorption Ability ◽  
Alginate Gel ◽  
Initial Stage ◽  
Intra Particle Diffusion ◽  
Pass Through ◽  
Komagataeibacter Xylinus

For reducing side effects and improvement of swallowing, we studied the encapsulation of activated carbon formulations with a hollow-type spherical bacterial cellulose (HSBC) gel using two kinds of encapsulating methods: Methods A and B. In Method A, the BC gelatinous membrane was biosynthesized using Komagataeibacter xylinus (K. xylinus) at the interface between the silicone oil and cell suspension containing activated carbon. In Method B, the bacterial cellulose (BC) gelatinous membrane was formed at the interface between the cell suspension attached to the alginate gel containing activated carbon and the silicone oil. After the BC gelatinous membrane was biosynthesized by K. xylnus, alginate gel was removed by soaking in a phosphate buffer. The activated carbon encapsulated these methods could neither pass through the BC gelatinous membrane of the HSBC gel nor leak from the interior cavity of the HSBC gel. The adsorption ability was evaluated using indole, which is a precursor of the uremic causative agent. From curve-fitting, the adsorption process followed the pseudo-first-order and intra-particle diffusion models, and the diffusion of the indole molecules at the surface of the encapsulated activated carbon within the HSBC gel was dominant at the initial stage of adsorption. It was observed that the adsorption of the encapsulated activated carbon by the intraparticle diffusion process became dominant with longer adsorption times.

Capability of Bacterial Cellulose Membrane in Release of Doxycycline

2016 ◽  
Vol 5 (1) ◽  
pp. 44-55 ◽  
Author(s):  
Mohammad Ali Salehi ◽  
Masomeh Jahani Kadusarai ◽  
Mojtaba Akbari Dogolsar
Keyword(s):  
Bacterial Cellulose ◽  
Future Research ◽  
Cellulose Membrane ◽  
Bacterial Product ◽  
Buffer Solution ◽  
Release Process ◽  
Cellulose Structure ◽  
Microbial Product ◽  
Bacterial Cellulose Membrane ◽  
Uv Visible

Bacterial cellulose produced by Gluconacetobacter xylinus is an advantage bacterial product cause of its unique properties, could be used as an ideal dress. The aim of this study was to consider the capability of this biomaterial in release of doxycycline. Proving of this capability provided the outline for production of a dressing containing antibiotic. In this study, bacterial cellulose was synthesized by G.xylinus and loaded from doxycycline then release process was considered in dilute water and buffer phosphate saline. UV-visible spectrophotometry method was applied to measuring the concentration of released drug. Bacterial cellulose structure of chemical was confirmed by Fourier Transform Infrared spectroscopy. Release of doxycycline in dilute water and phosphate saline buffer reach to 73.25% and 94.9%, respectively. Because of more released in buffer solution, peer that process of releasing exhibit exactly results. Results of this study provided the ground for future research on supplying an ideal dressing from this microbial product.

scholarly journals Assessing effectiveness of Komagataeibacter strains for producing surface-microstructured cellulose via guided assembly-based biolithography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marcello Brugnoli ◽  
Francesco Robotti ◽  
Salvatore La China ◽  
Kavitha Anguluri ◽  
Hossein Haghighi ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Medical Device ◽  
Soft Tissues ◽  
Acetic Acid Bacteria ◽  
Surface Pattern ◽  
Wild Type ◽  
Custom Made ◽  
Guided Assembly ◽  
Komagataeibacter Xylinus ◽  
Type Strains

AbstractIn this study, a medical device made of surface microstructured bacterial cellulose was produced using cellulose-producing acetic acid bacteria wild-type strains in combination with guided assembly-based biolithography. The medical device aims at interfering with the cell's focal adhesion establishment and maturation around implantable devices placed in soft tissues by the symmetrical array on its surface. A total of 25 Komagataeibacter strains was evaluated over a three-step selection. In the first step, the ability of strains to produce a suitable bacterial cellulose layer with high production yield was examined, then nine strains, with a uniform and smooth layer of bacterial cellulose, were cultured in a custom-made silicone bioreactor and finally the characteristics of the symmetrical array of topographic features on the surface were analysed. Selected strains showed high inter and intra species variability in bacterial cellulose production. The devices obtained by K2G30, K1G4, DSM 46590 (Komagataeibacter xylinus), K2A8 (Komagataeibacter sp.) and DSM 15973T (Komagataeibacter sucrofermentas) strains were pouched-formed with hexagonal surface pattern required for reducing the formation of fibrotic tissue around devices, once they are implanted in soft tissues. Our findings revealed the effectiveness of the selected Komagataeibacter wild-type strains in producing surface microstructured bacterial cellulose pouches for making biomedical devices.

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