Physical structure variations of bacterial cellulose produced by different Komagataeibacter xylinus strains and carbon sources in static and agitated conditions

Cellulose ◽  
2018 ◽  
Vol 25 (3) ◽  
pp. 1571-1581 ◽  
Author(s):  
Pratyawadee Singhsa ◽  
Ravin Narain ◽  
Hathaikarn Manuspiya
Keyword(s):  
Bacterial Cellulose ◽  
Carbon Sources ◽  
Physical Structure ◽  
Komagataeibacter Xylinus

scholarly journals Bacterial Cellulose (BC) and BC Composites: Production and Properties

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 192
Author(s):  
Tatiana G. Volova ◽  
Svetlana V. Prudnikova ◽  
Evgeniy G. Kiselev ◽  
Ivan V. Nemtsev ◽  
Alexander D. Vasiliev ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Synthesis Process ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Surface Culture ◽  
E Coli ◽  
Productive Process ◽  
Komagataeibacter Xylinus ◽  
Submerged Conditions ◽  
Properties Of Composites

The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m2 and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.

Tailoring bacterial cellulose structure through CRISPR interference‐mediated downregulation of galU in Komagataeibacter xylinus CGMCC 2955

2020 ◽  
Vol 117 (7) ◽  
pp. 2165-2176 ◽  
Author(s):  
Long‐Hui Huang ◽  
Qi‐Jing Liu ◽  
Xue‐Wen Sun ◽  
Xue‐Jing Li ◽  
Miao Liu ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Structure ◽  
Crispr Interference ◽  
Komagataeibacter Xylinus

Production of Bacterial Cellulose Using Different Carbon Sources by Two-Stage Cultivation Strategy

2011 ◽  
Vol 201-203 ◽  
pp. 722-725 ◽  
Author(s):  
Jun Hong Lin ◽  
Yi Jen Lin ◽  
Jui Chih Kuo ◽  
Ting Yu Chen ◽  
Wen Pei Sung
Keyword(s):  
Bacterial Cellulose ◽  
Reducing Sugars ◽  
Cell Concentration ◽  
Carbon Sources ◽  
Gluconacetobacter Xylinus ◽  
Glucose Medium ◽  
Two Stage ◽  
Second Stage ◽  
Cultivation Strategy ◽  
Cell Volumes

The production of bacterial cellulose (BC) from Gluconacetobacter xylinus could be improved by the two-stage cultivation strategy. The jar fermentor was applied at first stage to increase cell concentration. At the second stage, the bacteria statically grew within beaker. Three different fraction of cell volumes were cultured in modified YPD-glucose medium or YPD-molasses one. In the modified YPD-molasses medium, the BC yield were highest in this study when the consuming rate of reducing sugars (RS) was 0.181 g-dry BC L-1 d-1 and the BC production rate was 0.183 g-dry BC/g-RS, respectively. The modified YPD-glucose medium could get the maximum pellicle thickness of 5.56±0.64 mm and water content of 99.4%, respectively.

scholarly journals Production enhancement of bacterial cellulose nanofiber using local Komagataeibacter xylinus SB3.1 under static conditions

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Ahmed Alemam ◽  
Tharwat Shaheen ◽  
Saad El-Din Hassan ◽  
Said Desouky ◽  
Mamdouh El-Gamal
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Nanofiber ◽  
Komagataeibacter Xylinus ◽  
Static Conditions

scholarly journals Encapsulation of Activated Carbon into a Hollow-Type Spherical Bacterial Cellulose Gel and Its Indole-Adsorption Ability Aimed at Kidney Failure Treatment

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1076
Author(s):  
Toru Hoshi ◽  
Masahito Endo ◽  
Aya Hirai ◽  
Masashige Suzuki ◽  
Takao Aoyagi
Keyword(s):  
Activated Carbon ◽  
Cell Suspension ◽  
Bacterial Cellulose ◽  
Silicone Oil ◽  
Adsorption Ability ◽  
Alginate Gel ◽  
Initial Stage ◽  
Intra Particle Diffusion ◽  
Pass Through ◽  
Komagataeibacter Xylinus

For reducing side effects and improvement of swallowing, we studied the encapsulation of activated carbon formulations with a hollow-type spherical bacterial cellulose (HSBC) gel using two kinds of encapsulating methods: Methods A and B. In Method A, the BC gelatinous membrane was biosynthesized using Komagataeibacter xylinus (K. xylinus) at the interface between the silicone oil and cell suspension containing activated carbon. In Method B, the bacterial cellulose (BC) gelatinous membrane was formed at the interface between the cell suspension attached to the alginate gel containing activated carbon and the silicone oil. After the BC gelatinous membrane was biosynthesized by K. xylnus, alginate gel was removed by soaking in a phosphate buffer. The activated carbon encapsulated these methods could neither pass through the BC gelatinous membrane of the HSBC gel nor leak from the interior cavity of the HSBC gel. The adsorption ability was evaluated using indole, which is a precursor of the uremic causative agent. From curve-fitting, the adsorption process followed the pseudo-first-order and intra-particle diffusion models, and the diffusion of the indole molecules at the surface of the encapsulated activated carbon within the HSBC gel was dominant at the initial stage of adsorption. It was observed that the adsorption of the encapsulated activated carbon by the intraparticle diffusion process became dominant with longer adsorption times.

scholarly journals Bacterial Cellulose: Production, Modification and Perspectives in Biomedical Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1352 ◽  
Author(s):  
Gorgieva ◽  
Trček
Keyword(s):  
Bacterial Cellulose ◽  
Bone Repair ◽  
High Surface ◽  
Carbon Sources ◽  
High Surface Area ◽  
Young Modulus ◽  
High Water ◽  
Ex Situ ◽  
Bacterial Strains ◽  
High Flexibility

Abstract: Bacterial cellulose (BC) is ultrafine, nanofibrillar material with an exclusive combination of properties such as high crystallinity (84%–89%) and polymerization degree, high surface area (high aspect ratio of fibers with diameter 20–100 nm), high flexibility and tensile strength (Young modulus of 15–18 GPa), high water-holding capacity (over 100 times of its own weight), etc. Due to high purity, i.e. absence of lignin and hemicellulose, BC is considered as a non-cytotoxic, non-genotoxic and highly biocompatible material, attracting interest in diverse areas with hallmarks in medicine. The presented review summarizes the microbial aspects of BC production (bacterial strains, carbon sources and media) and versatile in situ and ex situ methods applied in BC modification, especially towards bionic design for applications in regenerative medicine, from wound healing and artificial skin, blood vessels, coverings in nerve surgery, dura mater prosthesis, arterial stent coating, cartilage and bone repair implants, etc. The paper concludes with challenges and perspectives in light of further translation in highly valuable medical products.

Modulation of the Structures and Properties of Bacterial Cellulose Film by Fermentation Carbon Sources

2013 ◽  
Vol 647 ◽  
pp. 160-164
Author(s):  
Guang Yang ◽  
Jian Jian Xie
Keyword(s):  
Bacterial Cellulose ◽  
Wound Dressing ◽  
Carbon Sources ◽  
Water Diffusion ◽  
Water Holding Capacity ◽  
Swelling Ratio ◽  
Pure Cellulose ◽  
Structures And Properties ◽  
Water Swelling ◽  
Water Holding

Bacterial cellulose (BC), a natural pure cellulose synthesized by some bacteria, shows great potentials for wound dressing applications. In order to obtain suitable properties, different fermentation carbon sources, i.e. glucose, maltose and sucrose were used to synthesize BC films by static culture. The crystallinity and pore analysis was performed by X-ray diffraction spectroscopy and nitrogen physisorption measurements, respectively. Some important properties of BC materials from different carbon sources were investigated, such as the mechanical strength, water holding capacity, water swelling ratio and water diffusion ability, which were key parameters for wound dressing applications. For comparison, the conventional cotton fiber was used as control. It was found that the carbon sources could change both the structures and properties of BC. The sucrose-derived BC exhibited lower crystallinity, but more suitable mechanical property, higher water holding capacity and water swelling ratio than other samples. All tested BC samples showed higher water holding capacity and water swelling ratio, but lower water diffusion property than the cotton fibers which were expected as good wound-healing biomedical materials.

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