scholarly journals Optimizing nanoencapsulation of Heracleum Lasiopetalum by response surface methodology

2021 ◽  
Author(s):  
Mahsa Yazdan-Bakhsh ◽  
Mojtaba Nasr-Esfahani ◽  
reza esmaeilzadeh kenari ◽  
Mohamad Fazel-Najafabadi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Food Systems ◽  
Whey Protein Concentrate ◽  
High Yield ◽  
Lepidium Sativum ◽  
Coating Materials ◽  
Seed Gum ◽  
Nanometric Size ◽  
Gradual Release

This study was carried out to optimize formulation for Heracleum Lasiopetalum (golpar) extract nanoencapsulation by response surface methodology (RSM). The primary emulsion was fabricated by (5-10 %) golpar extract (GE), (40-35 %) emulsifier span 80 (EM), and (50-60 %) sunflower oil (SO). The coating materials of nanoencapsulation were the composition of Lepidium sativum seed gum (LSG) and whey protein concentrate (WPC) at different ratios (1:0, 1:1, and 0:1). The yield of nanoencapsulation of golpar extract, particle size, and zeta potential was investigated as responses of RSM. The optimal formulation for nanoencapsulation of golpar extract were SO: 50.46%, GE 9.52%, and EM: 36.30% in LSG, SO: 57.07%, GE: 7.12%, and EM: 30.85% in LSG:WPC, and SO: 54.98%, GE: 9.05%, and EM: 39.87% in WPC coating. In conclusion, the nanoencapsulation of golpar extract prepared with the optimized formulation by RSM ensures the gradual release and sedimentation during storage with nanometric size and high yield of encapsulation. The nanocapsules of golpar extract can be used as a natural antioxidant in food systems.

Optimization of the Fermentation Conditions for 1-Deoxynojirimycin Production by Streptomyces lawendulae Applying the Response Surface Methodology

2011 ◽  
Vol 7 (3) ◽  
Author(s):  
Zhao-Jun Wei ◽  
Le-Chun Zhou ◽  
Hua Chen ◽  
Gui-Hai Chen
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Starch Content ◽  
Ultra Violet ◽  
Soluble Starch ◽  
High Yield ◽  
Yield Strain ◽  
Fermentation Time ◽  
Ethyl Sulfate ◽  
Initial Ph

Moranoline (1-Deoxynojirimycin, DNJ) is a piperidine alkaloid, and shows high inhibit activities to glucoamylase and ?-glucosidase. One DNJ high-yield strain of Streptomyces lawendulae was obtained after isolated form soil and mutated with the ultra violet (UV) and ethyl sulfate (DES), which named as TB-412, and can produce DNJ with 35.925 mg/L. Response surface methodology (RSM) was applied to optimize the parameters of DNJ yield from S. lawendulae TB-412. The effects of independent variables of fermentation, including time, temperature, initial pH and the soluble starch content were investigated. The statistical analysis showed that the fermentation time, pH and the soluble starch content, and the quadratics of time, temperature, pH and the soluble starch content, as well as the interactions between fermentation time and pH, and time and the soluble starch content, showed significant effects on DNJ yield. The optimal process parameters for DNJ production within the experimental range of the variables researched was at 11d, 27 °C, pH 7.5, and 8% soluble starch content. At this condition, the DNJ yield was predicted to be 42.875 mg/L.

scholarly journals Development, Characterization, and Optimization of Protein Level in Date Bars Using Response Surface Methodology

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Muhammad Nadeem ◽  
Salim-ur-Rehman ◽  
Faqir Muhammad Anjum ◽  
Mian Anjum Murtaza ◽  
Ghulam Mueen-ud-Din
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Whey Protein ◽  
Protein Level ◽  
Protein Supplementation ◽  
Interactive Effects ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Protein Isolates ◽  
Nutritional Profile

This project was designed to produce a nourishing date bar with commercial value especially for school going children to meet their body development requirements. Protein level of date bars was optimized using response surface methodology (RSM). Economical and underutilized sources, that is, whey protein concentrate and vetch protein isolates, were explored for protein supplementation. Fourteen date bar treatments were produced using a central composite design (CCD) with 2 variables and 3 levels for each variable. Date bars were then analyzed for nutritional profile. Proximate composition revealed that addition of whey protein concentrate and vetch protein isolates improved the nutritional profile of date bars. Protein level, texture, and taste were considerably improved by incorporating 6.05% whey protein concentrate and 4.35% vetch protein isolates in date bar without affecting any sensory characteristics during storage. Response surface methodology was observed as an economical and effective tool to optimize the ingredient level and to discriminate the interactive effects of independent variables.

Preparation of Lycopene Emulsions by Whey Protein Concentrate and Maltodextrin and Optimization by Response Surface Methodology

2014 ◽  
Vol 36 (2) ◽  
pp. 274-283 ◽  
Author(s):  
A. Salimi ◽  
Y. Maghsoudlou ◽  
S. M. Jafari ◽  
A. Sadeghi Mahoonak ◽  
M. Kashaninejad ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

Optimization of physical parameters for phycobiliprotein extracted from Oscillatoria agardhii and Synechococcus nidulans / Oscillatoria agardhii ve Synechococcus nidulans türlerinden fikobiliprotein ekstraksiyonu için fiziksel parametrelerin optimizasyonu

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
İrem Deniz ◽  
Esra İmamoğlu ◽  
Meltem Conk Dalay
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Light Intensity ◽  
Response Surface ◽  
Process Parameters ◽  
High Yield ◽  
Physical Parameters ◽  
Agitation Rate ◽  
Orbital Shaking ◽  
Oscillatoria Agardhii

AbstractObjective: Physical process parameters play a major role in the cultivation of cyanobacteria to provide high yield. The aim of this study was to optimize physical parameters such as light intensity and agitation rate which might affect the phycobiliprotein formations for cyanobacterial strains of Oscillatoria agardhii and Synechococcus nidulans using response surface methodology.Methods: The cyanobacterial strains were cultured in 250 mL flasks containing 100 mL of EM medium in orbital shaking incubator under the temperature of 22±2°C at different light intensities and agitation rates for 10 days. The experimental design was carried out using 2Results: The optimization solution of O. agardhii (approximately at 156 rpm under the light intensity of 65 μmol photons mConclusion: High agitation rate stimulated the faster growth than increased the light intensity for the growths of cyanobacterial strains.

scholarly journals Production of antioxidant hydrolyzates from a whey protein concentrate with thermolysin: Optimization by response surface methodology

LWT ◽  
2011 ◽  
Vol 44 (1) ◽  
pp. 9-15 ◽  
Author(s):  
María del Mar Contreras ◽  
Blanca Hernández-Ledesma ◽  
Lourdes Amigo ◽  
Pedro J. Martín-Álvarez ◽  
Isidra Recio
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Whey Protein ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

Process Optimization of Ultrasound-Assisted Extraction of Pectin from Helianthus annuus L. Heads by Response Surface Methodology

2014 ◽  
Vol 955-959 ◽  
pp. 848-854
Author(s):  
Yin Xiang Gao ◽  
Lei Yang ◽  
Yuan Gang Zu ◽  
Li Ping Yao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Helianthus Annuus ◽  
Response Surfaces ◽  
Quadratic Model ◽  
High Yield ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

An ultrasound-assisted procedure for the extraction of pectin from heads ofHelianthus annuusL. (sunflower) was established. A Box–Behnken design (BBD) was employed to optimize the extraction temperature (X1: 30–50°C), extraction time (X2: 20–40 min) and pH (X3: 2.5–3.5) to obtain a high yield of pectin with high degree of esterification (DE) from sunflower heads. Analysis of variance showed that the contribution of a quadratic model was significant for the pectin extraction yield and DE. An optimization study using response surface methodology was performed and 3D response surfaces were plotted from the mathematical model. According to the RSM model, the highest pectin yield (23.11 ± 0.08%) and DE (39.85 ± 0.14%) can be achieved when the UAE process is carried out at 50°C for 40min using a hydrochloric acid solution of pH 3.0. These results suggest that ultrasound-assisted extraction could be a good option for the extraction of functional pectin from sunflower heads at industrial level.

scholarly journals Efficient media for high production of microbial lipase from Bacillus subtilis (BSK-L) using response surface methodology for enantiopure synthesis of drug molecules

2020 ◽  
Author(s):  
Indu Bhushan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Industrial Applications ◽  
Bioactive Molecules ◽  
High Yield ◽  
Racemic Mixture ◽  
Potential Candidate ◽  
Chiral Drugs ◽  
Microbial Lipase ◽  
Pharmaceutical Industries

Lipases are a multipurpose enzyme that holds a significant position in industrial applications due to its ability to catalyse a large number of reactions such as hydrolysis, esterification, interesterification, transesterification which makes it a potential candidate. It is also used for the separation of chiral drugs from the racemic mixture and this property of lipase is considered very important in pharmaceutical industries for the synthesis of enantiopure bioactive molecules. Assuming the tremendous importance of lipases, as stereoselective biocatalysts, in pharmaceuticals and various other commercial applications, industrial enzymologists have been forced to search for those microorganisms which are able to produce novel biocatalysts at reasonably high yield. In the present study microbial lipase was isolated from the water sample of pond at Katra, Jammu and Kashmir (India). This enzyme has shown wide specificity and higher enantioselectivity, which make it pharmaceutical important enzyme. To make it economical for industrial application, it was produced on cheap nutrient media using Response Surface Methodology and got maximum production. It was used for resolution of chiral drugs and the significant results obtained during the course of work shall have potential towards pharmaceutical industries.

Boosting Mechanical Properties of Orthoses - Foot Ankle by Adding Carbon Nanotube Particles

2021 ◽  
Vol 1039 ◽  
pp. 518-536
Author(s):  
Abbas H. Jeryo ◽  
Jumaa S. Chiad ◽  
Wajdi S. Abbod
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Model Building ◽  
Fatigue Tests ◽  
Bending Test ◽  
Vacuum System ◽  
High Yield ◽  
Fatigue Properties ◽  
The Matrix ◽  
Two Parameters

In this process, optimum laminating properties were used in producing prosthesis and orthoses were researched and selected based on high yield, ultimate stresses, stresses of bending and fatigue properties. The process of the optimal selection is the Response Surface Methodology (RSM), which has been used to reach two parameters: reinforcement perlon fiber and percent of multi-strand carbon MWCNT nanotube combined with the matrix resin. The response surface methodology is a combination of mathematician and statistic techniques which are used for experimental model building and analysis of problems. This technique revealed 13 separate laminations samples with a percentage of separate Perlon layers No. and MWCNT Wt %. Tests were conducted for all lamination materials as defined in RSM methods and rendered by vacuum system, including fatigue tests for the ideal laminating material as opposed to laminations developed in the prior study (three Tensile test, Bending test and Fatigue tests according to the ASTM D638 and D790 respectively). Tests from the system version 10.0.2 of Design Expert found lamination (10 perlon layers and 0.75% of MWCNTs) to be the best according to overall yield, ultimate and bending loads in the 12 other laminations. Fatigue eventually revealed that constraints were applied to the stamina tension (2,66, 1,66) for optimum lamination, relative to ten perlon lamination layers and 424 lamination respectively.

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