Chromatographic Separation and Identification of Many Fatty acids and Phenolic Compounds from Flowers of Celosia cristata L. and Its Inhibitory Effect on Some Pathogenic Bacteria

The antimicrobial action of the dietary supplement Oxidal® was tested using the classic Bauer and Kirby agar-gel diffusion method. Clinical and reference strains of Staphylococcus aureus and Escherichia coli were used in the studies. The tested dietary supplement showed a well-pronounced inhibitory effect against the microbial strains commensurable with that of the broad-spectrum chemotherapeutic agent Enrofloxacin and showed even higher activity than the broad spectrum antibiotic Thiamphenicol. The proven inhibitory effect of the tested dietary supplement against the examined pathogenic bacteria is in accordance with the established clinical effectiveness standards for antimicrobial agents.

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Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

Current Analytical Chemistry ◽

10.2174/1573411016999200729111951 ◽

2020 ◽

Vol 16 ◽

Author(s):

Natasa P. Kalogiouri ◽

Natalia Manousi ◽

Erwin Rosenberg ◽

George A. Zachariadis ◽

Victoria F. Samanidou

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

High Performance ◽

Unsaturated Fatty Acids ◽

Nutritional Deficiencies ◽

Analytical Technique ◽

High Volatility ◽

Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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The Molecular Characterization and Biological Assessment of the Leaves Extracts of Loofah Reveal their Nutraceutical Potential

Recent Patents on Food Nutrition & Agriculturee ◽

10.2174/2212798411999201110211931 ◽

2020 ◽

Vol 11 ◽

Author(s):

Takoua Ben Hlel ◽

Feten Belhadj ◽

Fatih Gül ◽

Muhammed Altun ◽

Ayşe Şahin Yağlıoğlu ◽

...

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Hela Cell ◽

Arid Region ◽

Biological Properties ◽

Biological Assessment ◽

Omega 3 ◽

Hela Cell Lines ◽

Semi Arid Region ◽

Semi Arid

Background:: Luffa cylindrica is a plant that is widely distributed in Africa and Asia and it can be grown in regions with tropical or subtropical climates. Few patents dealt with Loofah biological properties, including some functional foods formulated from its leaves. Objective:: This study aimed to structurally and functionally characterize the bioactive compounds of L. cylindrica leaves grown in two different environments. Methods:: The extracts of L. cylindrica leaves collected from two Tunisian locations: Essouasi (LE), a semi-arid region and Medenine (LM) an arid region, were investigated for their phenolic compounds and fatty acids using HPLC/TOF-MS and GCMS techniques respectively. Furthermore, the antioxidant capacity was evaluated with DPPH, Chelating effect, Hydroxyl radical and Superoxide anion scavenging activities while the anticancer activity against HeLa cell lines was assessed using xCELLigence real time cell analyzer and lactate dehydrogenase cytotoxicity assay. Results:: The antiproliferative capacity of both extracts was time and dose-dependent with LE presenting the lowest HeLa cell index (CI = 0.035 ± 0.018, 250 μg/ml). LE also showed the best cytotoxic capacity (56.49 ± 0.8%) and antioxidant potential (IC50 = 54.41 ± 1.12 μg/ml for DPPH and 12.12 ± 0.07 μg/ml for chelating effet). 14 phenolic compounds were detected in LE with ferulic acid being the major compound (5128.5 ± 4.09 μg Phenols/g) while LM had only 6 phenolics. GCMS analysis showed the presence of omega-3 fatty acids in LE. Conclusions:: Our findings suggest that L. cylindrica leaves, especially when collected from semi-arid regions, are promising for formulating nutraceuticals of interest.

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Evaluation of antibacterial, teratogenicity and antibiofilm effect of sulfated chitosans extracted from marine waste against microorganism

Journal of Bioactive and Compatible Polymers ◽

10.1177/08839115211014225 ◽

2021 ◽

pp. 088391152110142

Author(s):

Velu Gomathy ◽

Venkatesan Manigandan ◽

Narasimman Vignesh ◽

Aavula Thabitha ◽

Ramachandran Saravanan

Keyword(s):

Biofilm Formation ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Inhibitory Effect ◽

Diffusion Method ◽

Marine Litter ◽

Ionization Time ◽

Gram Positive Bacteria ◽

Mineral Components ◽

Ft Ir

Biofilms play a key role in infectious diseases, as they may form on the surface and persist after treatment with various antimicrobial agents. The Staphylococcus aureus, Klebsiella pneumoniae, S. typhimurium, P. aeruginosa, and Escherichia coli most frequently associated with medical devices. Chitosan sulphate from marine litter (SCH-MW) was extracted and the mineral components were determined using atomic absorption spectroscopy (AAS). The degree of deacetylation (DA) of SCH was predicted 50% and 33.3% in crab and shrimp waste respectively. The elucidation of the structure of the SCH-MW was portrayed using FT-IR and 1H-NMR spectroscopy. The molecular mass of SCH-MW was determined with Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). The teratogenicity of SCH-MW was characterized by the zebrafish embryo (ZFE) model. Antimicrobial activity of SCH-MW was tested with the agar well diffusion method; the inhibitory effect of SCH-MW on biofilm formation was assessed in 96 flat well polystyrene plates. The result revealed that a low concentration of crab-sulfated chitosan inhibited bacterial growth and significantly reduced the anti-biofilm activity of gram-negative and gram-positive bacteria relatively to shrimp. It is potentially against the biofilm formation of pathogenic bacteria.

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Inhibitory effect of carboxylated nanodiamond on oral pathogenic bacteria Streptococcus mutans

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.23872 ◽

2021 ◽

Author(s):

Chuntian Quan ◽

Haiyan Lin ◽

Hui Xiao ◽

Jianjiang Zhao

Keyword(s):

Streptococcus Mutans ◽

Pathogenic Bacteria ◽

Inhibitory Effect

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Insight into the Secondary Metabolites of Geum urbanum L. and Geum rivale L. Seeds (Rosaceae)

Plants ◽

10.3390/plants10061219 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1219

Author(s):

Marek Bunse ◽

Peter Lorenz ◽

Florian C. Stintzing ◽

Dietmar R. Kammerer

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Unsaturated Fatty Acids ◽

Asiatic Acid ◽

Cosmetic Products ◽

Nutritional Properties ◽

First Time ◽

Derivatives Of ◽

Geum Urbanum ◽

Insight Into

The present study aimed at the identification and quantitation of phenolic compounds, fatty acids, and further characteristic substances in the seeds of Geum urbanum L. and Geum rivale L. For this purpose, individual components of extracts recovered with MeOH, CH2Cl2, and by cold-pressing, respectively, were characterized by HPLC-DAD/ESI-MSn and GC/MS and compared with reference compounds. For both Geum species, phenolic compounds, such as flavonoids and gallic acid derivatives, and triterpenes, such as saponins and their aglycones, were detected. Surprisingly, both Geum species revealed the presence of derivatives of the triterpenoid aglycons asiatic acid and madecassic acid, which were characterized for the first time in the genus Geum. Furthermore, the fatty acids of both species were characterized by GC–MS after derivatization. Both species showed a promising fatty-acid profile in terms of nutritional properties because of high proportions of unsaturated fatty acids. Linoleic acid and linolenic acid were most abundant, among other compounds such as palmitic acid and stearic acid. In summary, the present study demonstrates the seeds of G. urbanum and G. rivale to be a valuable source of unsaturated fatty acids and bioactive phenolics, which might be exploited for nutritional and cosmetic products and for phytotherapeutic purposes.

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Gas chromatographic separation of cholesteryl esters of fatty acids of different degrees of unsaturation

Lipids ◽

10.1007/bf02533299 ◽

1977 ◽

Vol 12 (2) ◽

pp. 228-232 ◽

Cited By ~ 13

Author(s):

Toru Takagi ◽

Akira Sakai ◽

Yutaka Itabashi ◽

Kenji Hayashi

Keyword(s):

Fatty Acids ◽

Chromatographic Separation ◽

Cholesteryl Esters ◽

Gas Chromatographic Separation

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Evidence for the regulation of guinea-pig heart microsomal phosphatidylcholine-hydrolysing phospholipase A1 by guanosine 5′-[γ-thio]triphosphate

Biochemical Journal ◽

10.1042/bj2880965 ◽

1992 ◽

Vol 288 (3) ◽

pp. 965-968 ◽

Cited By ~ 3

Author(s):

K Badiani ◽

X Lu ◽

G Arthur

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Guinea Pig ◽

Molecular Species ◽

Inhibitory Effect ◽

Phospholipase A1 ◽

Guinea Pig Heart ◽

Substrate Specificities ◽

Rate Of Hydrolysis ◽

Hydrolysis Of

We have recently characterized lysophospholipase A2 activities in guinea-pig heart microsomes and postulated that these enzymes act sequentially with phospholipases A1 to release fatty acids selectively from phosphatidylcholine (PC) and phosphatidylethanolamine, thus providing an alternative route to the phospholipase A2 mode of release. In a further investigation of the postulated pathway, we have characterized the PC-hydrolysing phospholipase A1 in guinea-pig heart microsomes. Our results show that the enzyme may have a preference for substrates with C16:0 over C18:0 at the sn-1 position. In addition, although the enzyme cleaves the sn-1 fatty acid, the rate of hydrolysis of PC substrates with C16:0 at the sn-1 position was influenced by the nature of the fatty acid at the sn-2 position. The order of decreasing preference was C18:2 > C20:4 = C18:1 > C16:0. The hydrolyses of the molecular species were differentially affected by heating at 60 degrees C. An investigation into the effect of nucleotides on the activity of the enzyme showed that guanosine 5′-[gamma-thio]triphosphate (GTP[S]) inhibited the hydrolysis of PC by phospholipase A1 activity, whereas GTP, guanosine 5′-[beta-thio]diphosphate (GDP[S]), GDP, ATP and adenosine 5′-[gamma-thio]triphosphate (ATP[S]) did not affect the activity. The inhibitory effect of GTP[S] on phospholipase A1 activity was blocked by preincubation with GDP[S]. A differential effect of GTP[S] on the hydrolysis of different molecular species was also observed. Taken together, the results of this study suggest the presence of more than one phospholipase A1 in the microsomes with different substrate specificities, which act sequentially with lysophospholipase A2 to release linoleic or arachidonic acid selectively from PC under resting conditions. Upon stimulation and activation of the G-protein, the release of fatty acids would be inhibited.

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