SNP Association in the Leptin Gene and Beef Quality Traits in Indigenous Sudanese Baggara Cattle

The present study was conducted on 112 Sudanese Baggara bulls (Nyalawi and Mesairi strains) from two separate locations in Darfur and Kordofan, Sudan, raised under dryland farming conditions. A single nucleotide polymorphism (C/T) Arg25Cys in exon 2 of the bovine leptin gene (NC_032653.1) was studied and the association of leptin genotypes with meat quality attributes was evaluated for these two Sudanese Baggara cattle strains which comprise the mainstay of Sudan export and local beef trade. The accuracy of genotyping was checked through PCR-RFLP technique followed by DNA sequencing and analyzed using BioEdit, MEGA6 and project Hope softwares. The genotype frequencies for CC, CT and TT genotypes in Nyalawi strain were 37.5, 39.3 and 23.2%, respectively, whereas the respective genotypic frequencies for Mesairi strain were 46.4, 28.6 and 25%. Significant differences (P<0.05) were found in hot carcass weight, dressing percentage, Myofibril fragmentation index (MFI), water holding capacity (WHC), cooking loss, moisture and fat between the two Baggara cattle strains. Association between the C>T SNP at the leptin gene and carcass weight, dressing and fat percentages was significant (P<0.05). It was concluded that Leptin gene polymorphisms contributed to the observed meat quality differences among these Sudanese cattle strains. This will allow for the use of molecular information in future selection of beef cattle in Sudan. The possible value of the leptin gene and its polymorphisms have been elucidated for the first time in Baggara cattle.

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Analysis of FTO and PLIN2 Polymorphisms in Relation to Carcass and Meat Quality Traits in Pigs

Annals of Animal Science ◽

10.2478/aoas-2018-0053 ◽

2019 ◽

Vol 19 (1) ◽

pp. 71-83 ◽

Cited By ~ 1

Author(s):

Daniel Polasik ◽

Eva-Maria Kamionka ◽

Mirosław Tyra ◽

Grzegorz Żak ◽

Arkadiusz Terman

Keyword(s):

Meat Quality ◽

Fat Content ◽

Longissimus Dorsi ◽

Quality Traits ◽

Large White ◽

Meat Quality Traits ◽

Pcr Rflp ◽

Perilipin 2 ◽

Water Holding ◽

Intramuscular Fat Content

AbstractThe aim of this study was to analyze the association of polymorphisms in alpha-ketoglutarate-dependent dioxygenase (FTO) and perilipin 2 (PLIN2) genes with carcass and meat quality traits in pigs reared in Poland. The research covered 578 sows that belong to the following breeds: Duroc, Hampshire, Polish Landrace, Pietrain, Puławska and Polish Large White. FTO (FM244720:g.400C>G) and PLIN2 (GU461317:g.98G>A) genes variants were determined by means of PCR-RFLP and ACRS-PCR methods respectively. Association between individual genotypes and analyzed traits was calculated by means of GLM procedure for Polish Landrace, Polish Large White and Puławska breeds separately and for all six breeds together in case of FTO gene. The results showed that FTO variants were associated with weight of loin without backfat and skin (WL), loin eye area (AL) and meat percentage (MP) in Polish Large White (P≤0.05), mean backfat thickness from 5 measurements (BFT) and pH measured 45 min after slaughter in m. longissimus dorsi (pH24 ld) as well as with water holding-capacity (WHC) in Puławska breed (P≤0.01). PLIN2 genotypes, however were correlated with WL and height of the loin eye (HL) in Polish Large White and Puławska (P≤0.05), AL in Polish Large White (P≤0.01) as well as luminosity (L*) in Puławska (P≤0.05) pigs. We observed most consistent relationships of PLIN2 SNP with intramuscular fat content (IMF) and WHC. In 3 analyzed breeds GG genotype was connected with highest values of these traits (P≤0.05).

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PCR-RFLP Polymorphism of Growth Hormone Gene and Its Association with Growth and Morphometric Traits in Ganjam and Baigani Goats of Odisha

Indian Journal of Animal Research ◽

10.18805/ijar.b-3971 ◽

2020 ◽

Author(s):

D. K. Karna ◽

A. Aradhana ◽

G. D. Nayak ◽

N. Sahoo ◽

L. K. Sahoo ◽

...

Keyword(s):

Growth Hormone ◽

Genetic Variation ◽

Restriction Enzyme ◽

Gene Segment ◽

Growth Hormone Gene ◽

Morphometric Traits ◽

Exon 2 ◽

Genotype Frequencies ◽

Significant Difference ◽

Pcr Rflp

Background: Growth Hormone (GH) gene codes for the growth hormone, an anabolic hormone synthesized and secreted by the somatotroph cells of pituitary. Growth hormone influences many processes such as growth, lactation, reproduction and metabolism. Genetic variation in the gene are utilized as markers for selecting animals that are superior in terms growth, production and reproduction traits. Information available on the polymorphism of growth hormone gene of Ganjam and Baigani goats of Odisha is scanty. The current investigation was done to explore the genetic variation of this gene and its association with morphometric traits. Methods: Genetic polymorphism in exon 2 and 3 of Growth hormone gene in Ganjam goat and Baigani goat was explored with sample size of 100 goats for each. The goats belonged to three locations: Khallikote, Rambha, Chattrapur of Ganjam district. The goats were recorded for their body weights, morphometric traits and morphological traits. Genomic DNA was isolated, the target segment comprising exons 2 and 3 was amplified and PCR-RFLP was carried out using Hae III restriction enzyme. Genotypes were scored. Result: PCR of the locus resulted in 422 bp PCR product. PCR-RFLP using Hae III restriction enzyme yielded only two variants in both the populations. The variant A had only one restriction recognition site on the target gene segment yielding two bands with size of 366bp and 56bp whereas the variant B did not have any restriction site with single band of 422bp. Three genotypes AA, AB and BB were found in both the population. In both the population, the gene and genotype frequency were significantly deviated from the Hardy Weinberg Equilibrium frequency. There was a significant difference in the genotype frequencies of growth hormone gene between Ganjam and Baigani goats. The AB genotype had higher mean value for all morphometric traits than AA and BB genotype though the differences were not found to be significant.

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Leptin Promoter Region Genotype Frequencies and Its Variability in the Czech Fleckvieh Cattle

Scientia Agriculturae Bohemica ◽

10.1515/sab-2016-0009 ◽

2016 ◽

Vol 47 (2) ◽

pp. 54-59 ◽

Cited By ~ 1

Author(s):

K. Jecminkova ◽

J. Kyselova ◽

A. Said Ahmed ◽

L. Zavadilova ◽

V. Matlova ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Resources ◽

Promoter Region ◽

Genetic Differences ◽

Genotype Frequencies ◽

Pcr Rflp ◽

First Time

Abstract To investigate the differences between the lineages of the Czech Fleckvieh cattle in Leptin promoter SNP C963T, 695 Czech Fleckvieh cows (650 from production herds and 45 from the Genetic Resources Program (GR)) were examined using PCR-RFLP. The C and T alleles of Leptin promoter were observed with a predominance of C allele in both groups. The most frequent genotypes were CC (63%) in production herds and CT (48%) in the GR population. The present study describes, for the first time, the genetic differences in production herds and GR population in Leptin promoter C963T SNP. Variation within the Czech Fleckvieh population was observed and resulted in an advantage to the GR population. Results presented herein emphasized the importance of the GR program as a reservoir of genetic diversity for indigenous breeds.

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Carcass traits and meat quality of quails from both sexes and eight distinct strains

Animal Production Science ◽

10.1071/an15854 ◽

2017 ◽

Vol 57 (10) ◽

pp. 2141 ◽

Cited By ~ 1

Author(s):

Ronan P. Gontijo ◽

Cleube A. Boari ◽

Aldrin V. Pires ◽

Martinho A. Silva ◽

Luíza R. A. Abreu ◽

...

Keyword(s):

Meat Quality ◽

Shear Force ◽

Carcass Traits ◽

Cooking Loss ◽

Water Holding Capacity ◽

Carcass Weight ◽

Water Holding ◽

Type Strains ◽

Meat Type

Our objective was to evaluate carcass traits and meat quality of quails from different strains and both sexes pertaining to three breeding-improvement programs. Quails from seven meat-type strains (UFV1, UFV2, UFV3, EV1, EV2, LF1 and LF2) and from one egg-laying (Japanese quail) strain (LAY) were slaughtered at 35 days of age. A completely randomised 8 (strain) × 2 (sex) factorial design was used for data analysis. Bodyweight, hot carcass weight and breast (musculus pectoralis major and m. pectoralis minor) and legs (thigh and drumstick) weights and their respective yields were determined. Ultimate pH, colour measurements, water-holding capacity, cooking loss and shear force were assessed. No difference in ultimate pH, shear force and hue was observed. The UFV3 strain had the greatest bodyweight at slaughter. The UFV3 strain had the greatest hot carcass weight in both sexes. The heaviest breasts were observed in both sexes of the UFV3 strain and in males of the UFV2 strain. The UFV3 quails had greater leg weight. The hot carcass yields of meat-type strains were greater than that of the LAY strain. The UFV3 and LAY females furnished a greater breast yield than did male quails. For males, the UFV1 strain had a greater yield of breast than that of LAY quails. The LAY quails presented the greatest yields of leg. The LAY quails had the least luminance, and the greatest water-holding capacity, cooking loss, red and yellow intensity, and chroma. In summary, meat obtained from meat-type strains had a greater water-holding capacity, lower cooking loss and greater luminance than that of the LAY strain. In addition, the meat-type strains grew appreciably faster, but the difference in yields of breast and leg meat between meat and layer strains was small. We conclude that meat-type strains, especially the UFV3, are more appropriate for enterprises focussed on meat production.

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Impact of Amaranthus hypochondriacus in nutrition for rabbits on meat quality

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.26756 ◽

2021 ◽

Vol 72 (1) ◽

pp. 2713

Author(s):

RS SHEVCHIK ◽

YV DUDA ◽

OG GAVRILINA ◽

LV KUNEVA ◽

HV SAMOYLUK

Keyword(s):

Moisture Content ◽

Meat Quality ◽

Live Weight ◽

Water Holding Capacity ◽

Carcass Weight ◽

Control Group ◽

Amaranthus Hypochondriacus ◽

Rabbit Meat ◽

The Impact ◽

Water Holding

Unique properties of Amaranthus hypochondriacus have been extensively utilized in the recent time worldwide both in food technologies and husbandry. An intensive growth of Amaranthus hypochondriacus plants has been made in Ukraine there and, respectively, production of foods based on amaranth is growing, however no research on its use for feeding rabbits is available. Therefore, the study aimed to review the impact of Amaranthus hypochondriacus on slaughter features of rabbits, meat quality including those under storage conditions. The California race rabbits of 60 days age were divided into two groups, 28 animals in each. The rabbits in the study group were fed (by adding to the basal diet) with 20% of amaranth oilcake. The rabbits were slaughtered at age of 120 days. The following key features of meat output and quality performance were determined: pH, moisture content, water-holding capacity, cooking losses, protein and cholesterol content as well as change in pH, microorganisms count, and moisture loss percentage during 9-day storage in chilled state. Amaranthus hypochondriacus effect on the rabbit live weight, hot carcass weight, dressing out percentage, percentage ratio of heart, kidney, liver, lung weight to carcass weight, рН, moisture content, water-holding capacity, and meat morphological structure was determined. Reduction in cholesterol level by 15.07% (р < 0.05) in the meat of rabbits that were fed with Amaranthus hypochondriacus was detected. pH and drip loss percentage were not significantly different among the groups in the shelf-life period. However, it was found out that growth of microorganisms in the meat of rabbits that were fed with Amaranthus hypochondriacus was slowed down: microorganisms count was 1.65 and 1.71 (р < 0.05) times lower than in the control group on the 6th and 9th days of storage, accordingly. The low-cholesterol and bacteriostatic effects of amaranth oilcake in the rabbit nutrition may significantly increase dietary properties of rabbit meat. Use of amaranth oilcake for rabbit fattening has great potential and further studies including the mechanism of antibacterial effect of A. hypochondriacus on the rabbit meat are required

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Identification of SNPs in ME1 gene and association analysis with meat quality traits in Chinese Red cattle

Czech Journal of Animal Science ◽

10.17221/7526-cjas ◽

2014 ◽

Vol 59 (No. 7) ◽

pp. 297-301

Author(s):

G.L. Zhou ◽

Y. Cao ◽

H.G. Jin

Keyword(s):

Statistical Analysis ◽

Meat Quality ◽

Quality Traits ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Exon 2 ◽

Coding Regions ◽

Meat Quality Traits ◽

Pcr Rflp ◽

Exon 4

The objective of this study was to identify single nucleotide polymorphisms (SNPs) in coding regions of bovine ME1 gene and to evaluate if the polymorphisms are associated with meat quality traits in Chinese Red cattle. Four SNPs were identified: NW_001495544:g.1721768G>A in exon 2, g.1653796T>A in exon 4, g.1649532G>A in exon 5, and g.1546272T>C in exon 12 and they were genotyped by applying the PCR-RFLP method. Statistical analysis showed that two SNPs, g.1649532G>A and g.1546272T>C, were significantly associated with cooking loss and pH<sub>24h</sub> (P < 0.05). But no statistically significant differences were observed in the g.1721768G>A and g.1653796A>T SNPs for meat quality traits tested in Chinese Red cattle. This suggests that ME1 gene is a candidate that may have effects on meat quality traits in cattle.

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Evaluation of meat quality characteristics of imported chilled lambs in Saudi Arabia

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.8463 ◽

2017 ◽

Author(s):

A. Al-Owaimer ◽

G. Suliman ◽

E. Hussein

Keyword(s):

Saudi Arabia ◽

Sensory Evaluation ◽

Meat Quality ◽

Quality Characteristics ◽

The Other ◽

Longissimus Dorsi ◽

Ether Extract ◽

Overall Acceptability ◽

Fragmentation Index ◽

Water Holding

This study was carried out to evalaute meat qaulity characteristics of imported chilled lambs in Saudi Arabia. Eighteen Longissimus dorsi (LD) muscle samples of three imported breeds (Sawakni, Pakistani and Australi) were used. Moisture and ether extract contents were significantly (P less than 0.05) different between the breeds. The breeds also showed significant (P less than 0.05) differences in yellowness (b*), water-holding capacity (WHC) and cooking loss percentage (CL). The best overall acceptability was showed by Australi breed followed by Pakistani. The superior WHC ratio was attained by Pakistani breed, while Austali breed showed the best CL%. The highest myofibril fragmentation index (MFI) value was scored by Pakistani breed followed by Australi. It is concluded that Australi breed was the best between the tested breeds followed by Pakistani and lastly Sawakni based on its superiority over the other two breeds in all sensory evaluation components.

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IDENTIFYING THE C677T POLYMORPHISM OF MTHFR GENE BY PCR-RFLP TECHNIQUE

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2011.2.3 ◽

2011 ◽

pp. 28-35

Author(s):

Keyword(s):

Internal Control ◽

Restriction Site ◽

Mthfr Gene ◽

C677t Polymorphism ◽

Mthfr Genotype ◽

Restriction Digest ◽

Genotype Frequencies ◽

Pcr Rflp ◽

Sequencing Technique ◽

Volunteer Group

Background: The C677T polymorphism of MTHFR gene is a risk factor of many diseases. This study is aimed at: (1) Improving a PCR-RFLP process with the own designed primers to identify the C677T polymorphism of MTHFR gene. (2) Evaluating the prevalence of the C677T polymorphism of MTHFR gene in volunteer group. Materials and method: DNA samples was extracted from peripheral blood of 60 volunteers. Designing primers by using FastPCR software, then improving PCR technique. Standardizing the optimal conditions of restriction digest by HinfI. Confirming the results of polymorphism by DNA sequencing technique. Results: We designed successfully primers to amplify fragment of MTHFR gene including C677T polymorphism and an obligatory restriction site of HinfI (as internal control). 0.5 µl of HinfI enzyme (10 U/µl) is enough for restriction digest. The MTHFR genotype frequencies were: 71.67 % (677CC); 25% (677CT); and 3.33 % (677TT). Conclusion: We standardized successfully PCR-RFLP technique to identifying C677T polymorphism of MTHFR gene. Keywords: C677T polymorphism, MTHFR gene, PCR-RFLP

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Estudio del polimorfismo y poligenismo de los loci DRB en caballos criollo argentino

10.35537/10915/4578 ◽

2003 ◽

Author(s):

◽

Silvina Díaz

Keyword(s):

Exon 2 ◽

Pcr Rflp

El objetivo del trabajo de Tesis Doctoral consistió en estudiar el polimorfismo y el poligenismo de los genes de clase II DRB del Complejo Principal de Histocompatibilidad en Equinos (ELA). La variabilidad genética de las regiones promotoras y de la región de reconocimiento del antígeno (exón 2) se analizó mediante los métodos de PCR-RFLP y PCR-SSCP. Se detectaron tres nuevos alelos del exón 2 definidos por PCR-RFLP, los que se confirmaron por clonado y secuenciación de los productos de amplificación. Además, el número de variantes detectadas en cada animal permitió inferir la presencia de al menos tres copias de genes DRB en los haplotipos equinos analizados. Estos datos se confirmaron a través de análisis filogenético y de segregación. El clonado y secuenciación de la región reguladora (URR) de los genes DRB permitió caracterizar la organización del promotor proximal. Los resultados obtenidos mostraron la presencia en dirección 5´ - 3´ de las cajas conservadas W, X, Y, CCAAT y TATA. Esta estructura es semejante a la reportada en los genes ortólogos de otras especies de mamíferos y evidenciaría que la región analizada corresponde a un gen DRB funcional. Por otra parte, el análisis del polimorfismo del promotor permitió identificar cinco alelos definidos por SSCP. El conjunto de resultados obtenidos mostró que los genes ELA-DRB presentan las principales características descriptas para los genes de Clase II de mamíferos: existencia de copias múltiples, alto grado de polimorfismo, alta tasa de sustituciones no sinónimas en los sitios de reconocimiento del antígeno, y conservación de secuencia y estructura del promotor proximal.

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5′ Region Large Genomic Rearrangements in the BRCA1 Gene in French Families: Identification of a Tandem Triplication and Nine Distinct Deletions with Five Recurrent Breakpoints

Cancers ◽

10.3390/cancers13133171 ◽

2021 ◽

Vol 13 (13) ◽

pp. 3171

Author(s):

Sandrine M. Caputo ◽

Dominique Telly ◽

Adrien Briaux ◽

Julie Sesen ◽

Maurizio Ceppi ◽

...

Keyword(s):

Brca1 Gene ◽

Genomic Rearrangements ◽

Comparative Genomic ◽

Homologous Region ◽

Exon 2 ◽

Large Genomic Rearrangements ◽

Causality Score ◽

Frequent Site ◽

First Time ◽

Intron 2

Background: Large genomic rearrangements (LGR) in BRCA1 consisting of deletions/duplications of one or several exons have been found throughout the gene with a large proportion occurring in the 5′ region from the promoter to exon 2. The aim of this study was to better characterize those LGR in French high-risk breast/ovarian cancer families. Methods: DNA from 20 families with one apparent duplication and nine deletions was analyzed with a dedicated comparative genomic hybridization (CGH) array, high-resolution BRCA1 Genomic Morse Codes analysis and Sanger sequencing. Results: The apparent duplication was in fact a tandem triplication of exons 1 and 2 and part of intron 2 of BRCA1, fully characterized here for the first time. We calculated a causality score with the multifactorial model from data obtained from six families, classifying this variant as benign. Among the nine deletions detected in this region, eight have never been identified. The breakpoints fell in six recurrent regions and could confirm some specific conformation of the chromatin. Conclusions: Taken together, our results firmly establish that the BRCA1 5′ region is a frequent site of different LGRs and highlight the importance of the segmental duplication and Alu sequences, particularly the very high homologous region, in the mechanism of a recombination event. This also confirmed that those events are not systematically deleterious.

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