scholarly journals Antioxidative enzymes during germination of two lines of Serbian spruce [Picea omorika (Panc.) Purkyně]

2007 ◽  
Vol 59 (3) ◽  
pp. 209-216 ◽  
Author(s):  
Olivera Prodanovic ◽  
R. Prodanovic ◽  
Jelena Bogdanovic ◽  
Aleksandra Mitrovic ◽  
N. Milosavic ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidative Enzymes ◽  
Peroxidase Activity ◽  
Enzyme Activities ◽  
Specific Activity ◽  
Germination Percentage ◽  
Isoenzyme Pattern ◽  
Picea Omorika ◽  
Serbian Spruce

Two lines of Picea omorika (Panc.) Purkyn? were compared with respect to germination percentage as well as specific activity and isoenzyme pattern of catalase, superoxide dismutase, and peroxidase (POD) during germination. Line A had a higher germination percentage and higher enzyme activities in dry seeds and seedlings compared to line C. Peroxidase activity was not detected in dry seeds, but measured up to 10 U/g and 28 U/g on the 7th day of germination in lines C and A, respectively. The most abundant POD basic isoform in seedlings of both lines (pI 8.2) was not found previously in needles of adult Serbian spruce trees of the same lines. .

scholarly journals Effect of Salicylic Acid on the Growth, Photosynthetic Efficiency and Enzyme Activities of Leguminous Plant under Cadmium Stress

2014 ◽  
Vol 42 (2) ◽  
pp. 440-445 ◽  
Author(s):  
Mohammed Naseer ALYEMENI ◽  
Qaiser HAYAT ◽  
Leonard WIJAYA ◽  
Shamsul HAYAT
Keyword(s):  
Superoxide Dismutase ◽  
Salicylic Acid ◽  
Antioxidative Enzymes ◽  
Enzyme Activities ◽  
Photosynthetic Efficiency ◽  
Cadmium Stress ◽  
Leguminous Plant ◽  
Exogenous Application ◽  
Catalase Peroxidase ◽  
Cd Exposure

The present study was designed with an objective to elucidate the effect of the exogenous application of 10-5 M of salicylic acid (SA) to the foliage of the chickpea plants exposed to 0, 25, 50 or 100 mg cadmium (Cd) per kg of soil. The foliage of the plants grown in soil amended with varying doses of Cd was sprayed with 10-5 M of SA at 30 days after sowing (DAS). The plant samples were collected at 60 and 90 DAS to assess various parameters. The damage caused due to the Cd exposure was partially overcome by the exogenous application of SA. The SA treatment resulted in a significant increase of growth, photosynthesis, yield and the activity of antioxidative enzymes viz. catalase, peroxidase and superoxide dismutase in the plants exposed to 0, 25 or 50 mg Cd per kg of soil. However, the treatment did not prove to be fruitful in alleviating completely the stress generated by 100 mg Cd per kg of soil.

scholarly journals Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence

2011 ◽  
Vol 63 (2) ◽  
pp. 399-405 ◽  
Author(s):  
N.S. Stanisavljevic ◽  
Dragana Nikolic ◽  
Z.S. Jovanovic ◽  
Jelena Samardzic ◽  
Svetlana Radovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidative Enzymes ◽  
Enzyme Activities ◽  
Antioxidative Defense ◽  
Defense System ◽  
System Activity ◽  
Antioxidative Enzyme Activities ◽  
Fagopyrum Esculentum Moench

Oxidative stress and antioxidative defense system activity were studied in buckwheat leaves after complete submergence and re-aeration. The levels of H2O2 and lipid peroxidation were found to be significantly higher in stressed than in untreated buckwheat leaves. Enzymes catalyzing the degradation of H2O2 and peroxides were shown to participate actively, whereas superoxide dismutase did not take part in the buckwheat leaf response to flooding stress. The most prominent increase in antioxidative enzyme activities was noticed upon return to air, when the strongest oxidative stress occurred and the need for antioxidative defense was the greatest.

scholarly journals Antioxidant enzymes in the needles of different omorika lines

2005 ◽  
Vol 57 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Jelena Bogdanovic ◽  
Tanja Ducic ◽  
Nenad Milosavic ◽  
Z. Vujcic ◽  
Mirjana Sijacic ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Polyphenol Oxidase ◽  
Seed Orchard ◽  
Peroxidase Isoenzymes ◽  
Coniferous Species ◽  
Soluble Peroxidase ◽  
Picea Omorika ◽  
Serbian Spruce ◽  
Isoenzyme Patterns

Picea omorika (Panc) Purkyn? (Serbian spruce) is a Balkan endemic coniferous species. We studied soluble peroxidase, catalase, polyphenol oxidase, and superoxide-dismutase activity in the needles of five omorika lines grown in a generative seed orchard. The peroxidase and polyphenol oxidase isoenzyme patterns were also investigated. Activity of the studied enzymes varied among different lines. The highest activity of peroxidase, catalase and polyphenol oxidase was found in the A3 ("borealis") and B5 ("semidichotomous") lines. Four acidic and two basic peroxidase isoenzymes and one polyphenol oxidase isoenzyme were detected. There was no variation in either the peroxidase or the polyphenol oxidase isoenzyme pattern among the different lines.

scholarly journals Multiple forms of superoxide dismutase in the apoplast and whole-needle extract of Serbian spruce [Picea omorika (Panc.) Purkyně]

2006 ◽  
Vol 58 (4) ◽  
pp. 211-214 ◽  
Author(s):  
Jelena Bogdanovic ◽  
R. Prodanovic ◽  
N. Milosavic ◽  
Olivera Prodanovic ◽  
Ksenija Radotic
Keyword(s):  
Superoxide Dismutase ◽  
Soluble Fraction ◽  
Detectable Amount ◽  
Multiple Forms ◽  
Sod Activity ◽  
Total Extract ◽  
Picea Omorika ◽  
Isoenzyme Composition ◽  
Serbian Spruce ◽  
Specific Inhibitors

Activity and isoenzyme composition of superoxide dismutase in the apoplast and whole-needle extract of Picea omorika (Panc.) Purkyn?, was studied. Total SOD activity of the soluble fraction of the needle extract exceeded markedly that of the apoplastic SOD. Several acidic and two slow-migrating basic isoforms were found in the whole extract. Extracellular SOD had an extremely acidic isoform. Using specific inhibitors, we identified Cu/Zn- and MnSOD forms in the total extract, but only MnSOD in the apoplast. The Feisoform was not present in a detectable amount.

scholarly journals Phytochemical changes in mango fruit in response to Alternaria alternata infection

2018 ◽  
Vol 36 (No. 3) ◽  
pp. 227-232 ◽  
Author(s):  
Jiao Li ◽  
Liangang Mao ◽  
Yanning Zhang ◽  
Lan Zhang ◽  
Hongyun Jiang
Keyword(s):  
Superoxide Dismutase ◽  
Vitamin C ◽  
Fruit Quality ◽  
Alternaria Alternata ◽  
Enzyme Activities ◽  
Phenylalanine Ammonia Lyase ◽  
Titratable Acidity ◽  
Mango Fruit ◽  
Malondialdehyde Content

Changes in mango fruit quality, malondialdehyde content, and enzymatic activities in response to pathogen Alternaria alternata infection were studied. A. alternata significantly affected the appearance of mango fruit at 5 and 7 days after treatment (DAT). The quality of pathogen-infected fruit first showed a significant decrease in titratable acidity and vitamin C content and a significant increase in pH since 3 DAT. The malondialdehyde content was higher than that in the untreated controls at 3 and 7 DAT. The enzyme activities of ascorbate peroxidase and polyphenol oxidase showed significant increases since 3 DAT. Significant increases in l-phenylalanine ammonia-lyase and superoxide dismutase activities were observed at 7 DAT. These results indicate that A. alternata infection first significantly affects some biochemical constituents and enzyme activities in mango fruit since 3 DAT and that there was no significant effect on appearance until 5 DAT.

scholarly journals In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

2013 ◽  
Vol 64 (4) ◽  
pp. 553-559 ◽  
Author(s):  
Seyed Fazel Nabavi ◽  
Solomon Habtemariam ◽  
Antoni Sureda ◽  
Akbar Hajizadeh Moghaddam ◽  
Maria Daglia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin C ◽  
Gallic Acid ◽  
Sodium Fluoride ◽  
Enzyme Activities ◽  
Control Group ◽  
Rat Erythrocytes ◽  
Pre Treatment

Abstract Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m-3 for one week). Gallic acid at 10 mg kg-1 and 20 mg kg-1 and vitamin C for positive controls (10 mg kg-1) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes.

Cytokines increase rat lung antioxidant enzymes during exposure to hyperoxia

1989 ◽  
Vol 66 (2) ◽  
pp. 1003-1007 ◽  
Author(s):  
C. W. White ◽  
P. Ghezzi ◽  
S. McMahon ◽  
C. A. Dinarello ◽  
J. E. Repine
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Tumor Necrosis ◽  
Interleukin 1 ◽  
Antioxidant Enzyme Activities ◽  
Rat Lung ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Necrosis Factor

Pretreatment with the combination of tumor necrosis factor/cachectin (TNF/C) and interleukin 1 (IL-1) increased glucose-6-phosphate dehydrogenase (G6PDH), glutathione reductase (GR), glutathione peroxidase (GPX), catalase (CAT), and superoxide dismutase (SOD) activities in lungs of rats continuously exposed to hyperoxia for 72 h, a time when all untreated rats had already died. Pretreatment with TNF/C and IL-1 also increased, albeit slightly, lung G6PDH and GR activities of rats exposed to hyperoxia for 4 or 16 h. By comparison, no differences occurred in lung antioxidant enzyme activities of TNF/C and IL-1- or saline-pretreated rats exposed to hyperoxia for 36 or 52 h; the latter is a time just before untreated rats began to succumb during exposure to hyperoxia. The results raise the possibility that TNF/C and IL-1 treatment can increase lung antioxidant enzyme activities and that increased lung antioxidant enzymes may contribute to the increased survival of TNF/C and IL-1-pretreated rats in hyperoxia for greater than 72 h.

scholarly journals Bicarbonate-dependent Peroxidase Activity of Human Cu,Zn-Superoxide Dismutase Induces Covalent Aggregation of Protein

2003 ◽  
Vol 278 (26) ◽  
pp. 24078-24089 ◽  
Author(s):  
Hao Zhang ◽  
Christopher Andrekopoulos ◽  
Joy Joseph ◽  
Karunakaran Chandran ◽  
Hakim Karoui ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Peroxidase Activity

scholarly journals Thermal dependence of the antioxidant enzymes superoxide dismutase, catalase, and peroxidase in foliage of Iris pumila L.

2009 ◽  
Vol 61 (3) ◽  
pp. 441-446 ◽  
Author(s):  
Ana Vuleta ◽  
Branka Tucic
Keyword(s):  
Activation Energy ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Temperature Increase ◽  
Specific Activity ◽  
Thermal Dependence ◽  
Full Sunlight ◽  
Iris Pumila ◽  
Temperature Optima

Thermal dependence of the enzymes SOD, CAT, and POD was investigated in leaves of Iris pumila plants inhabiting two contrasting light environments, a sun-exposed dune site and a woodland understory. At the same assay temperature, both the specific activity and the activation energy of SOD and CAT were higher in plants inhabiting vegetation shade than in those experiencing full sunlight. Conversely, the temperature optima for the two enzymes did not differ between alternative radiation environments. The specific activity of POD increased with temperature increase, and was always greater in plants growing under full sunlight than in those from vegetation shade. The activation energy of POD was higher than that of SOD or CAT, being lower in sun-than in shade-exposed plants.

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