scholarly journals Screening of antibacterial activity of raspberry (Rubus idaeus L.) fruit and pomace extracts

2012 ◽  
pp. 305-313 ◽  
Author(s):  
Aleksandra Velicanski ◽  
Dragoljub Cvetkovic ◽  
Sinisa Markov
Keyword(s):  
Antibacterial Activity ◽  
Wild Strain ◽  
Rubus Idaeus ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wild Strains ◽  
Agar Well Diffusion Method

Antibacterial activity of fruit and pomace extracts (concentration 50 mg/ml) of two raspberry (Rubus idaeus L.) cultivars (Meeker and Willamette) was tested against selected Gram-positive and Gram-negative bacteria (reference and wild strains). Disc diffusion method with 15 ?l of extracts and agar-well diffusion method with 50 and 100 ?l were used. Antibiotic (cefotaxime/clavulanic acid) was used as a control. Both raspberry fruit extracts showed the strongest antibacterial activity against Pseudomonas aeruginosa (wild strain) and Bacillus cereus, where the largest clear zones (without growth) appeared. Escherichia coli was the most resistant strain, with only zone of reduced growth. The highest antibacterial activity of pomace extracts was against Staphylococcus aureus and Staphylococcus saprophyticus. There were no differences in the antibacterial activity between cultivars for both fruit and pomace extracts.

scholarly journals A Study on Antibacterial Activity of Silver Nanoparticles

2018 ◽  
Vol 29 (2) ◽  
pp. 37-41
Author(s):  
M Iqbal Hossain ◽  
M Anwar Habib ◽  
Nazimuddin Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Reference Drug ◽  
Gram Negative ◽  
Quasi Experimental

This quasi experimental study was designed to evaluate antibacterial activity of chemically synthesized silver nanoparticles (AgNPs) from silver nitrate (AgNO3) solutions on gram negative bacteria like E.Coli using disc diffusion method. Different concentrations of AgNPs, AgNO3 and reference drug ciprofloxacin were used to find out the antibacterial activity which revealed that AgNPs possessed significant antibacterial effect compared to AgNO3 solutions but relatively less antibacterial effect than that of ciprofloxacin. So chemical synthesis guided AgNPs may have some antibacterial effects.TAJ 2016; 29(2): 37-41

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

scholarly journals Synthesis and Antimicrobial Assessment of Fe3+ Inclusion Complex of p-tert-Butylcalix[4]arene Diamide Derivative

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Anwar Ali Chandio ◽  
Ayaz Ali Memon ◽  
Shahabuddin Memon ◽  
Fakhar N. Memon ◽  
Qadeer Khan Panhwar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Ft Ir ◽  
Bacteria And Fungi

Present study deals with the synthesis of the p-tert-butylcalix[4]arene diamide derivative as ligand (L) and its Fe3+ complex, followed by its characterization using TLC and FT-IR, while UV-Vis and Job’s plot study were performed for complex formation. Antimicrobial activity of the derivative (L) and its metal complex was carried out by the disc diffusion method against bacteria (Escherichia coli and Staphylococcus albus) and fungi (R. stolonifer). Different concentrations of the derivative (L) (6, 3, 1.5, 0.75, and 0.37 μg/mL) and its Fe3+ complex were prepared, and Mueller–Hinton agar was used as the medium for the growth of microorganisms. Six successive dilutions of the derivative (L) and Fe3+ complex were used against microorganisms. Two successive dilutions (6 and 3 μg/mL) of the derivative (L) showed antibacterial action against both Gram-positive and Gram-negative bacteria. In addition, three successive dilutions (6, 3, and 1.5 μg/mL) of the derivative (L) showed antifungal activity. However, all of six dilutions of the Fe3+ complex showed antimicrobial activity. Derivative (L) showed 3 and 1.5 μg/mL minimum inhibitory concentrations (MIC) against bacteria and fungi, respectively. On the contrary, its Fe3+ complex showed 0.37 μg/mL value of MIC against bacteria and fungi. Hence, Fe3+ complex of the derivative (L) was found to be a more effective antimicrobial agent against selected bacteria and fungi than the diamide derivative (L).

scholarly journals New Diarylheptanoid from Garuga pinnata Roxb

2013 ◽  
Vol 61 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Mst Taslima Khatun ◽  
M. Mahboob Ali Siddiqi ◽  
Al-Mansur MA ◽  
MH Sohrab ◽  
AFM Mustafizur Rahman ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Microbial Growth ◽  
Stem Bark ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Wide Range ◽  
Bacteria And Fungi

9´-Desmethylgaruganin I has been isolated from the dichloromethane extract of the stem bark of Garuga pinnata Roxb. The crude extract was screened for antimicrobial activity against a wide range of gram-positive and gram-negative bacteria and fungi by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The dichloromethane extract showed moderate inhibitory activity to microbial growth and weak cytotoxicity having LC90 25.703?g-mL–1 DOI: http://dx.doi.org/10.3329/dujs.v61i2.17058 Dhaka Univ. J. Sci. 61(2): 131-134, 2013 (July)

scholarly journals Gölbaşı ve Azaplı Göllerinden (Adıyaman) İzole Edilen Bakterilerin Tiplendirilmesi ve Çoklu Antibiyotik Dirençliliklerinin Araştırılması

2017 ◽  
Vol 5 (1) ◽  
pp. 43
Author(s):  
Fikret Büyükkaya Kayış ◽  
Sadık Dinçer ◽  
Fatih Matyar ◽  
Hatice Aysun Mercimek Takcı ◽  
Melis Sümengen Özdenefe ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Seasonal Changes ◽  
Reference Value ◽  
Summer Season ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Multiple Antibiotic Resistance ◽  
Disc Diffusion Method ◽  
Gram Negative

Identification and multiple antibiotic resistances of amphicillin, chloramphenicol, streptomycin and tetracycline resistant gram-negative bacteria that isolated microorganisms from Gölbaşı and Azaplı lakes (Adiyaman) were investigated in this study. Seasonally taken isolates of totally 386 bacteria in 10 different species from 7 genera were scanned against 16 antibiotics [gentamycin, imipenem, kanamycin, chloramphenicol, meropenem, nalidixic acid, nitrofurantoin, penicillin, cephalothin, cefazolin, cefpirome, ceftizoxime, cefuroxime, streptomycin, tetracycline and trimethoprim-sulphamethoxazole (Bioanalyse)] by using the disc diffusion method to determine the prevalence of multiple antibiotic resistance. Multiple antibiotic resistance of stations showed seasonal changes between 0.29 and 0.91. In generally, multiple antibiotic resistance in Golbasi and Azapli lakes were higher than the reference value and highest multiple antibiotic resistance values were obtained at summer season (3th period). When the obtained data are considered, high multiple antibiotic resistance poses a risk in terms of public health and for economically important animals.

SYNTHESIS OF PIPERINE - PIPERAZINE ANALOGUES AND THEIR ANTIBACTERIAL ACTIVITY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 30-35
Author(s):  
Naveen K. Kottakki ◽  
◽  
Amperayani K. Rao ◽  
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Aromatic Nitro ◽  
Heterocyclic Moiety ◽  
Agar Well Diffusion Method

In the current study, a series of piperine – piperazine analogues (5a to 5f) were designed and synthesized. The piperine was isolated from pepper and used for the conjugation with heterocyclic moiety for better biological activity. The piperazine heterocyclic was chosen for conjugation with piperine. The newly synthesized structures were determined by IR, 1H NMR and 13C NMR spectral data. The compounds were examined for their anti‐microbial activity against gram-positive (Bacillus subtilis) and gram-negative (Vibrio cholerae) bacteria using the agar well diffusion method. The newly synthesized compounds exhibited capable activities against V. cholerae and B. subtilis and it showed minimum inhibitory concentration. Among all the synthesized compounds, 5f has the highest activity (26 mm) against gram-positive bacteria and (29mm) against gram-negative bacteria. The remaining compounds showed appreciable antibacterial activity. The enhanced activity of the synthesized compounds may be due to the presence of conjugated amide linkage with the natural product piperine and piperazine heterocyclic molecule. The substituents present on the aromatic (nitro-substituted) ring also influenced the activity of the compound.

Phenotypic Characterisation of Klebseilla pneumoniae Carbapenemase and Metallo Beta-lactamase in Carbapenem Resistant Gram-negative Bacteria

2021 ◽  
Author(s):  
Thresia . ◽  
Surya Sankar ◽  
Siju Joseph ◽  
V.R. Ambily ◽  
Anu Bosewell ◽  
...  
Keyword(s):  
Confirmatory Test ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Beta Lactamase ◽  
Disc Diffusion ◽  
Beta Lactam ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
E Coli ◽  
Carbapenem Resistant

Background: Antibiotic resistance is an emerging concern in the therapy of clinical infections worldwide. Previous studies conducted in our laboratory have confirmed an increase in the prevalence of extended spectrum beta-lactamase (ESBL) among the Gram-negative bacterial pathogens associated with dogs, which could act as a potential source for the transfer of these resistant pathogens or their genetic determinants to human. Since carbapenems are the last resort drugs against these resistant pathogens, the study was aimed to isolate and characterise carbapenem resistance among Escherichia coli (E. coli), Klebsiella pneumoniae (K. pneumoniae) and Pseudomonas aeruginosa (P. aeruginosa) associated with common clinical infections in dogs.Methods: A total of 100 samples were collected from lesional skin, urine and anterior vagina of dogs presented to the Veterinary Hospitals of Kerala Veterinary and Animal Sciences University at Mannuthy and Thrissur. The samples were cultured onto Brain Heart Infusion Agar (BHIA), Eosin Methylene Blue (EMB) and Mac Conkey (MAC) for isolation of bacteria. Identification of the isolates was performed based on cultural, morphological and biochemical characteristics. The isolates were subjected to antimicrobial susceptibility test (ABST) against the 12 commonly used beta-lactam and non–beta-lactam group of antibiotics by disc diffusion method and further subjected to screening for ESBL double disc diffusion method. Carbapenem-resistant isolates were subjected to phenotypic confirmatory test for carbapenemase production employing Imipenem-EDTA and Ertapenem-boronic acid minimum inhibitory concentration (MIC) strip method.Result: Forty four Gram-negative bacterial isolates obtained were viz., E. coli (30), K. pneumonia (11) and P. aeruginosa (3) from the 100 samples. Apart from these, other isolates obtained were Staphylococcus spp. (53) and Bacillus spp. (2). All the Gram-negative isolates were subjected to ABST employing 12 common antibiotics belonging to beta-lactam and non-beta-lactam groups. Multidrug resistance (MDR) could be observed in 28 E. coli, 11 K. pneumoniae and three P. aeruginosa isolates. All the 42 MDR isolates showed positive results for ESBL production. A total of 14 isolates out of the 44 Gram-negative bacilli were found to be resistant to carbapenem either to imipenem, meropenem or ertapenem. Among the 14 Gram-negative isolates, nine turned out to be positive for metallo-beta-lactamase (MBL) and none for K. penumoniae carbapenemase (KPC) on phenotypic confirmatory test for detecting major carbapenemase enzymes. The present study documented that Gram- negative bacteria like E. coli, K. pneumoniae and P. aeruginosa isolated from dogs are showing an increase rate of resistance against carbapenems which are the last resort drugs against ESBL producers. Hence, there is an urgent need to curb the irrational and excessive use of antibiotics in veterinary sector.

scholarly journals Antimicrobial Activity of Some Indigenous Plants of Bangladesh

1970 ◽  
Vol 7 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Mohammad S Rahman ◽  
Mohammed Z Rahman ◽  
Md Abdul Wahab ◽  
Rasheduzzaman Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Indigenous Plants ◽  
Gram Negative ◽  
Agar Disc ◽  
Bacteria And Fungi

The antimicrobial activity of methanol extracts of 17 plant species of Bangladesh was evaluated by the agar disc diffusion method. Among those, eight plant extracts exhibited potent antimicrobial activity against a wide variety of Gram positive and Gram negative bacteria and fungi at a concentration of 400 μg /disc.Key words: Antimicrobial activity, Disc diffusion method.DOI = 10.3329/dujps.v7i1.1213Dhaka Univ. J. Pharm. Sci. 7(1): 23-26, 2008 (June)

scholarly journals Evaluation of Some Common Disinfectants Against Some Gram-Negative Bacteria

2020 ◽  
Vol 10 (01) ◽  
pp. 102-107
Author(s):  
Ghada A. Mohammad
Keyword(s):  
Bacterial Growth ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Citrobacter Freundii ◽  
Disc Diffusion ◽  
Bacterial Isolates ◽  
Disc Diffusion Method ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Inhibition Ability

Seven disinfectants were used in this study, green clean, luxxtol, bittol, PAX guard, SALi, sarttol, and lenol, and their inhibitory activity was determined against nine gram-negative bacterial isolates; one isolate of each Escherichia coli and Acinetobacter baumannii, two isolates of each Klebsiella pneumoniae, and Pseudomonas aeruginosa, and three isolates of Citrobacter freundii, by using disc diffusion method. Results demonstrated the efficacy of lenol because it was the only disinfectant that gave inhibition zones around the filter disc for all types of studied bacteria, but other disinfectants had different effects on bacterial growth. As well as, the detection on effect of prolonged disinfectant time by exposing bacteria to disinfectant for ten minutes expecting increased efficiency of disinfectants. The results showed that bittol had the first rank in increasing its inhibition ability of bacterial growth, Green clean was the next, followed by SALi, Sartol. Lastly, Luxxtol had the last rank because it had few effects on just A. baumannii.

Antibacterial activity of magnesium oxide nanoparticles prepared by Calcination method

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Elaf Ayad Kadhem ◽  
Miaad Hamzah Zghair ◽  
Sarah , Hussam H. Tizkam, Shoeb Alahmad Salih Mahdi ◽  
Hussam H. Tizkam ◽  
Shoeb Alahmad
Keyword(s):  
Antibacterial Activity ◽  
Magnesium Oxide ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Magnesium Oxide Nanoparticles ◽  
Agar Disc

magnesium oxide nanoparticles (MgO NPs) were prepared by simple wet chemical method using different calcination temperatures. The prepared NPs were characterized by Electrostatic Discharge (ESD), Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD). It demonstrates sharp intensive peak with the increase of crystallinty and increase of the size with varying morphologies with respect to increase of calcination temperature. Antibacterial studies were done on gram negative bacteria (E.coli) and gram positive bacteria (S.aureus) by agar disc diffusion method. The zones of inhibitions were found larger for gram positive bacteria than gram negative bacteria, this mean, antibacterial MgO NPs activity more active on gram positive bacteria than gram negative bacteria because of the structural differences. It was found that antibacterial activity of MgO NPs was found it has directly proportional with their concentration.

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