Influence of fatty acids and detergents on polyether antibiotic production by Streptomyces hygroscopicus NRRL B-1865

Addition of pure methyl oleate to a Streptomyces hygroscopicus NRRL B-1865 culture improved polyether antibiotic production, particularly of the closely related nigericin and abierixin, more than did technical methyl oleate. Among the fatty acid constituents of technical methyl oleate, the methyl esters of stearate and particularly linoleate were detrimental to growth and antibiotic production, while the methyl esters of palmitate and palmitoleate had the same effect as technical methyl oleate. The presence of Tween 80 or cyclodextrin in S. hygroscopicus cultures supplemented with methyl oleate increased valine uptake and antibiotic production. Antibiotic production was highest with a mixture of 1% methyl oleate, 0.1% Tween 80, and 0.1% cyclodextrin. The results suggested that the stimulatory effect of methyl oleate might be enhanced by a substance increasing the accumulation of branched amino acids known to be direct precursors of polyether antibiotics. The rate of abierixin synthesis was more important than that of nigericin at the end of the culture. The nigericin/abierixin ratio decreased from 6 in basal medium supplemented with methyl oleate to 2 in basal medium supplemented with methyl oleate, Tween 80, and cyclodextrin. A strong decrease in culture pH seen in the presence of Tween 80 and cyclodextrin might lead to a Michael transformation of nigericin to abierixin.Key words: Streptomyces hygroscopicus, nigericin, abierixin, methyl oleate, Tween 80, cyclodextrin.

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Multifunctional lubricant additives derived from natural amino acids and methyl oleate

RSC Advances ◽

10.1039/c5ra15239a ◽

2015 ◽

Vol 5 (95) ◽

pp. 77538-77544 ◽

Cited By ~ 14

Author(s):

Arukali Sammaiah ◽

Korlipara V. Padmaja ◽

Shiva Shanker Kaki ◽

Rachapudi B. N. Prasad

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Methyl Oleate ◽

Thioglycolic Acid ◽

Methyl Esters ◽

Lubricant Additives ◽

Acid Methyl ◽

Multifunctional Additives ◽

Natural Amino Acids ◽

Amino Acid Methyl Esters

Novel multifunctional additives were synthesized from methyl oleate via thioglycolic acid addition followed by condensation with different amino acid methyl esters.

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Biochemical composition of two giant pill-millipedes of the Western Ghats of India

Biological Letters ◽

10.1515/biolet-2015-0014 ◽

2015 ◽

Vol 52 (1-2) ◽

pp. 45-61 ◽

Cited By ~ 2

Author(s):

Cheviri N. Ambarish ◽

Kandikere R. Sridhar

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Western Ghats ◽

Unsaturated Fatty Acids ◽

Essential Fatty Acids ◽

Methyl Esters ◽

Essential Amino Acids ◽

Essential Elements ◽

Western Ghats Of India ◽

The Western Ghats

AbstractMany invertebrates have an unexpected nutraceutical potential and are of nutritional or ethnomedicinal significance to many tribals throughout the world. The giant pill-millipedes of the genus Arthrosphaera are traditionally used as natural medicines by tribals in the Western Ghats of India. In this study, two species of pill-millipedes (Arthrosphaera fumosa and A. magna) were subjected to proximate and biochemical analysis to ascertain their nutritional potential. Bodies of A. fumosa and A. magna (after removal of their intestines) had a low protein content (8-15%) and high quantity of carbohydrates (40-41%). They were rich in many essential elements, especially in calcium. The essential amino acids of pillmillipedes were in high quantities. The level of glycine was the highest, followed by lysine and serine. The fatty acid methyl esters (FAMEs) of males and females consist of high quantities of unsaturated fatty acids. The mono-unsaturated fatty acids were more abundant than poly-unsaturated fatty acids. Palmitic and oleic acids were dominant saturated and unsaturated fatty acids, respectively. The study has revealed for the first time that pill-millipedes of the Western Ghats of India constitute a good source of essential minerals, essential fatty acids, and essential amino acids. Being valuable contributors of organic manure by processing recalcitrant plant lignocellulosic wastes, pill-millipedes become part and parcel of organic farming as well as future nutraceutical sources.

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Performance of GC-MS analysis for differentiation of various types of flour by creating dendrogram of liposoluble extract

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120209030v ◽

2012 ◽

Vol 18 (4-1) ◽

pp. 555-561 ◽

Cited By ~ 8

Author(s):

Djura Vujic ◽

Marijana Acanski ◽

Marija Bodroza-Solarov ◽

Nikola Hristov ◽

Milica Krunic

Keyword(s):

Mass Spectrometry ◽

Fatty Acids ◽

Cluster Analysis ◽

Gas Chromatography ◽

Methyl Oleate ◽

Methyl Palmitate ◽

Methyl Esters ◽

Fatty Acids Composition ◽

Different Types ◽

Small Grains

Gas chromatography with mass spectrometry was used for performing a qualitative analysis of liposoluble flour extract made of different types of small grains (bread wheat, barley and triticale). The results show that the dominant methyl-esters of fatty acids composition is same for all samples, but the percentage of their representation in various types of small grains is different. Transesterification reagent was TMSH (Trimethylsulfonium hydroxide, 0.2M in methanol). With transesterification reaction fatty acids from acilglycerol esterifyed to methyl-esters. In all extract analyzed methyl linoleate predominated, followed by methyl palmitate, methyl oleate and methyl stearate in decreasing order. The following tests cluster analysis was used for the comparison of the liposoluble flour extract different types of small grains.

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Antibiotic production by Streptomyces hygroscopicus CH-7 in medium containing Schiff base complexes

Hemijska industrija ◽

10.2298/hemind181016008i ◽

2019 ◽

Vol 73 (2) ◽

pp. 93-101

Author(s):

Slavica Ilic ◽

Sandra Konstantinovic ◽

Gordana Gojgic-Cvijovic ◽

Vlada Veljkovic

Keyword(s):

Crude Glycerol ◽

Antibiotic Production ◽

Basal Medium ◽

Divalent Metal ◽

Biodiesel Production ◽

Streptomyces Hygroscopicus ◽

Schiff Base Complexes ◽

Spectral Studies ◽

Divalent Metal Ions ◽

Modified Media

Influence of a modified media on Hexaene H-85 and Azalomycine B production by Streptomyces hygroscopicus CH-7 was investigated. The amino acid L-tryptophan, as a nitrogen source, was replaced with isatin-3-thiosemicarbazone and its complexes with some divalent metal ions. Isatin-3-thiosemicarbazone was synthesized in crude glycerol obtained as a byproduct in biodiesel production from sunflower oil. The complexes were charac-terized by elemental microanalysis and magnetic susceptibility, as well as, by Atomic absorption(AA), Fourier-transform infrared (FTIR) and Ultraviolet?visible (UV/VIS) spectro-scopic methods. The spectral studies indicated an octahedral geometry for the Mn(II), Fe(II) and Ni(II) complexes and a tetrahedral one for the Zn(II) complex. Comparing to the basal medium, isatin-3-thiosemicarbazone (ITC) and its metal complexes in the concentration of 0.5 g dm-3 showed better results in the antibiotics production. Use of medium supple-mented with the Fe(II) complex resulted in the maximum Hexaene H-85 and Azalomycine B concentrations of 306 ?g cm-3 and 127 ?g cm-3, respectively. Addition of ITC and its complexes changed the morphology of S. hygroscopicus CH-7 from filaments to pellets as a dominant shape in media resulting in higher antibiotic production.

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Fatty acid requirement of Treponema denticola and Treponema vincentii

Canadian Journal of Microbiology ◽

10.1139/m82-051 ◽

1982 ◽

Vol 28 (3) ◽

pp. 344-350 ◽

Cited By ~ 6

Author(s):

Kenneth G. Van Horn ◽

Robert M. Smibert

Keyword(s):

Fatty Acids ◽

Oleic Acid ◽

Fatty Acid ◽

Basal Medium ◽

Tween 80 ◽

Treponema Denticola ◽

Good Growth ◽

Maximal Growth ◽

Lipid Component ◽

Palmitic Acids

Treponema denticola and Treponema vincentii were cultured in a medium supplemented with either 0.2 or 0.4% (w/v) alpha globulin in place of serum. The active factor(s) in alpha globulin was stable at pH 7.0 to autoclaving and was nondialyzable. Extraction of lipids from alpha globulin showed that both protein and lipid, supplied by the alpha globulin, were required for maximal growth of these two oral treponemes. The lipid component was investigated by adding sodium salts of long-chain fatty acids to the basal medium supplemented with 0.4% delipified alpha globulin. The lipid component of alpha globulin was replaced by either oleic acid (cis-18:1 (9)) or by elaidic acid (trans-18:1 (9)). No other saturated or unsaturated fatty acid tested could support good growth. Tween 80 (polysorbitan monooleate) was the only Tween compound able to support maximal growth of T. denticola. The cellular lipids of T. denticola, grown with oleate in broth supplemented with 0.4% delipified alpha globulin, were extracted and analyzed by gas chromatography. The principle fatty acids were myristic, pentadecanoic, and palmitic acids. Lesser amounts of oleic acid, eicosadienoic acid, and an unidentified fatty acid (retention time, 88 min) were also detected. Treponema denticola appears to be capable of limited synthesis of cellular fatty acids such as myristic, pentadecanoic, and palmitic acids from oleic acid.

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The influence of Schiff base inclusion complexes with β-cyclodextrine on antibiotic production by Streptomyces hygroscopicus CH-7

Hemijska industrija ◽

10.2298/hemind131113012i ◽

2015 ◽

Vol 69 (1) ◽

pp. 9-15

Author(s):

Slavica Ilic ◽

Sandra Konstantinovic ◽

G. Gojgic-Cvijovic ◽

Dragisa Savic ◽

Vlada Veljkovic

Keyword(s):

Schiff Base ◽

Inclusion Complex ◽

Schiff Bases ◽

Inclusion Complexes ◽

Fermentation Process ◽

Antibiotic Production ◽

Basal Medium ◽

Streptomyces Hygroscopicus ◽

Nutrient Media ◽

The Media

A media consisting of isatin-Schiff bases and its inclusion complexes with ?-cyclodextrine was developed to maximize the production of antibiotics Hexaene H-85 and Azalomycine ? by Streptomyces hygroscopicus CH-7. The media with ?-cyclodextrine inclusion complex of isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 493 ?g/cm3 for Hexaene H-85 and 191 ?g/cm3 for Azalomycine B. The production of Hexaene H-85 and Azalomycine is higher when ?-cyclodextrine complex is added as a nitrogen source, comparing to pure isatin-Schiff base. The maximum concentration of hexaene H-85 in medium with inclusion complex of isatin-Schiff base is 1.4-2.3 times higher than the basal medium. The maximum production of Azalomycine is 2.1-3.4 times higher in media with inclusion complex of Schiff base. During the fermentation process, the nutrient media with ?-cyclodextrine inclusion complexes with isatin-Schiff bases affect the strain morphology, since it is in the form of compact pellets, which are formed from short and long, branched filaments

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Nutritional composition analysis of seaweed Sargassum crassifolium J. Agardh.

Biofarmasi Journal of Natural Product Biochemistry ◽

10.13057/biofar/f020201 ◽

2004 ◽

Vol 2 (2) ◽

pp. 45-52 ◽

Cited By ~ 1

Author(s):

TRI HANDAYANI ◽

SUTARNO SUTARNO ◽

AHMAD DWI SETYAWAN

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Vitamin A ◽

Vitamin C ◽

Extraction Method ◽

Methyl Esters ◽

Brown Seaweed ◽

Mineral Elements ◽

Nutritional Composition ◽

Composition Analysis

The aims of the research were to find out nutritional composition of seaweed Sargassum crassifolium J. Agardh i. e. concentration of protein, amino acids, mineral (ash), mineral elements (Ca, Fe, and P), vitamin C, vitamin A, lipid, fatty acids and alginates. S. crassifolium is a species of brown seaweed that is consumed as source of food, however, it have not optimally used due to the nutritional composition information does not complete yet. The measurement of protein concentration was done according to Lowry method, while amino acids concentration was measured using High Performance Liquid Chromatography (HPLC). Mineral (ash) was measured by dry ash processing, and mineral elements of Ca, Fe, and P were measured using atomic absorption spectrophotometer (AAS) and UV-Vis spectrophotometer. Vitamin C concentration was measured by titration method, while vitamin A was measured using UV-Vis spectrophotometer. Lipid was measured by extraction method using soxhlet, fatty acids by fatty acids methyl esters (FAMEs) method, and alginates were measured by extraction method. The results indicate that the thallus of S. crassifolium contain protein in the average of 5.19% (w/w), and 17 amino acids (in Î¼mol amino acid/g wet weight) varies from 13.77 of glutamic acid to 0.83 for hydroxilicine concentration. Mineral/ash content was 36.93% (w/w), Ca: 1540.66 mg/100 g, Fe: 132.65 mg/100 g, P: 474.03 mg/100 g, vitamin C: 49.01 mg/100 g, vitamin A: 489.11 Î¼g RE/100 g, lipid: 1.63% (w/w), fatty acids concentrations were: 1.45%, 3.53%, 29.49%, 4.10%, 13.78%, 33.58%, 5.94% for lauric acid, meristic acid, palmitic acid, palmitoleic acid, oleic acid, linoleic acid, and linolenic acid subsequently. The concentration of alginates was 37.91% (w/w).

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