scholarly journals Highly efficient production of Aspergillus niger amylase cocktail by solid-state fermentation using triticale grains as a well-balanced substrate

2015 ◽  
Vol 80 (11) ◽  
pp. 1375-1390 ◽  
Author(s):  
Biljana Dojnov ◽  
Marica Grujic ◽  
Zoran Vujcic
Keyword(s):  
Aspergillus Niger ◽  
Mineral Elements ◽  
Reducing Sugar ◽  
Efficient Production ◽  
Raw Starch ◽  
Simultaneous Production ◽  
Particle Size Increase ◽  
Raw Starch Hydrolysis ◽  
First Time ◽  
Glucose Formation

Triticale (Triticosecale, Wittmak) an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not sufficiently utilized yet. The simultaneous production of ?-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, has been developed, optimized and scaled up 10 fold for the first time. The specific combination of examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase of oxygen availability and substrate height lead to increase of amylases production of 30%. Reduction of relative humidity from 65% to 30% increased glucoamylase production 2 times and ?-amylase production for 30%. Peak production of ?-amylase (158 U/g) and glucoamylase (170 U/g) were obtained in erlenmayers and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient in raw starch hydrolysis from wheat flour, 29% more efficient in glucose formation and 10% more efficient in total reducing sugar formation, than commercially available amylase cocktail SAN Super 240L widely used in industry.

Biochemical Profiles of Various Fresh Extracts of Gnaphalium polycaulon Pers

2017 ◽  
Vol 9 (04) ◽  
Author(s):  
Shanmugapriya K. ◽  
T. Murugan ◽  
Thayumanavan Tha
Keyword(s):  
Biochemical Analysis ◽  
South India ◽  
Standard Procedure ◽  
Research Work ◽  
Reducing Sugar ◽  
Total Lipids ◽  
Total Proteins ◽  
Total Carbohydrates ◽  
Biochemical Profiles ◽  
First Time

Medicinal plants plays a significant role in the pharmaceutical industry. In present scenario, the need is to explore, identify and utilize this new medicinal plant on one hand and, on the other, to help conserve the existing but threatened species of rare medicinal plant.This present research work was carry out for the first time in South India to analyse and estimate the biochemical profiles of various extracts of fresh parts of Gnaphalium polycaulon pers., plant. The biochemical composition such as total carbohydrates, total proteins, total lipids, total phenols, cholesterol, total chlorophylls and reducing sugar, sterols were estimated using the standard procedure in fresh plant material.The biochemical analysis of Gnaphalium polycaulon plant showed the presence of various phytochemicals. The results of the present study supplement the usage of the studied plant which possesses several bioactive compounds and used as food and also as medicine.The results of present studies demonstrated that Gnaphalium polycaulon plant could be a sourceof valuable information and a guideline for the scientists, researchers in India and also all over the world

Effects of metal ions on simultaneous production of glucose oxidase and catalase by Aspergillus niger

2001 ◽  
Vol 32 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J.-Z. Liu ◽  
Y.-Y. Huang ◽  
J. Liu ◽  
L.-P. Weng ◽  
L.-N. Ji
Keyword(s):  
Aspergillus Niger ◽  
Metal Ions ◽  
Glucose Oxidase ◽  
Simultaneous Production

scholarly journals Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Morakot Krajang ◽  
Kwanruthai Malairuang ◽  
Jatuporn Sukna ◽  
Krongchan Rattanapradit ◽  
Saethawat Chamsart
Keyword(s):  
Ethanol Production ◽  
Ethanol Concentration ◽  
Consolidated Bioprocessing ◽  
Scale Up ◽  
Cassava Starch ◽  
Single Step ◽  
Starch Hydrolysis ◽  
Yield Coefficient ◽  
Raw Starch ◽  
Raw Starch Hydrolysis

Abstract Background A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigate the optimum conditions and technical procedures for the production of ethanol from raw cassava starch in a single step. It successfully resulted in high yields and productivities of all the experiments from the laboratory, the pilot, through the industrial scales. Yields of ethanol concentration are comparable with those in the commercial industries that use molasses and hydrolyzed starch as the raw materials. Results Before single-step ethanol production, studies of raw cassava starch hydrolysis by a granular starch hydrolyzing enzyme, StargenTM002, were carefully conducted. It successfully converted 80.19% (w/v) of raw cassava starch to glucose at a concentration of 176.41 g/L with a productivity at 2.45 g/L/h when it was pretreated at 60 °C for 1 h with 0.10% (v/w dry starch basis) of Distillase ASP before hydrolysis. The single-step ethanol production at 34 °C in a 5-L fermenter showed that Saccharomyces cerevisiae (Fali, active dry yeast) produced the maximum ethanol concentration, pmax at 81.86 g/L (10.37% v/v) with a yield coefficient, Yp/s of 0.43 g/g, a productivity or production rate, rp at 1.14 g/L/h and an efficiency, Ef of 75.29%. Scale-up experiments of the single-step ethanol production using this method, from the 5-L fermenter to the 200-L fermenter and further to the 3000-L industrial fermenter were successfully achieved with essentially good results. The values of pmax,Yp/s, rp, and Ef of the 200-L scale were at 80.85 g/L (10.25% v/v), 0.42 g/g, 1.12 g/L/h and 74.40%, respectively, and those of the 3000-L scale were at 70.74 g/L (8.97% v/v), 0.38 g/g, 0.98 g/L/h and 67.56%, respectively. Because of using raw starch, major by-products, i.e., glycerol, lactic acid, and acetic acid of all three scales were very low, in ranges of 0.940–1.140, 0.046–0.052, 0.000–0.059 (% w/v), respectively, where are less than those values in the industries. Conclusion The single-step ethanol production using the combination of raw cassava starch hydrolysis and fermentation of three fermentation scales in this study is practicable and feasible for the scale-up of industrial production of ethanol from raw starch.

Effects of metal ions on simultaneous production of glucose oxidase and catalase by Aspergillus niger

2008 ◽  
Vol 32 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J.-Z. Liu ◽  
Y.-Y. Huang ◽  
J. Liu ◽  
L.-P. Weng ◽  
L.-N. Ji
Keyword(s):  
Aspergillus Niger ◽  
Metal Ions ◽  
Glucose Oxidase ◽  
Simultaneous Production

A morphological, enzymatic and metabolic approach to elucidate apoptotic-like cell death in fungi exposed to h- and α-molybdenum trioxide nanoparticles

Nanoscale ◽  
2018 ◽  
Vol 10 (44) ◽  
pp. 20702-20716 ◽  
Author(s):  
Clemencia Chaves-Lopez ◽  
Hang N. Nguyen ◽  
Rodrigo C. Oliveira ◽  
Enrico T. Nadres ◽  
Antonello Paparella ◽  
...  
Keyword(s):  
Cell Death ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Molybdenum Trioxide ◽  
Fungal Strains ◽  
First Time

The present study compares for the first time the effects of h-MoO3and α-MoO3against two fungal strains:Aspergillus nigerandAspergillus flavus.

scholarly journals Switching from a Unicellular to Multicellular Organization in an Aspergillus niger Hypha

mBio ◽  
2015 ◽  
Vol 6 (2) ◽  
Author(s):  
Robert-Jan Bleichrodt ◽  
Marc Hulsman ◽  
Han A. B. Wösten ◽  
Marcel J. T. Reinders
Keyword(s):  
Aspergillus Niger ◽  
Fluorescent Protein ◽  
Stress Conditions ◽  
Physical Contact ◽  
Effective Control ◽  
Mobility Rate ◽  
Content Type ◽  
Green Fluorescent ◽  
First Time ◽  
Compound Concentration

ABSTRACT Pores in fungal septa enable cytoplasmic streaming between hyphae and their compartments. Consequently, the mycelium can be considered unicellular. However, we show here that Woronin bodies close ~50% of the three most apical septa of growing hyphae of Aspergillus niger. The incidence of closure of the 9th and 10th septa was even ≥94%. Intercompartmental streaming of photoactivatable green fluorescent protein (PA-GFP) was not observed when the septa were closed, but open septa acted as a barrier, reducing the mobility rate of PA-GFP ~500 times. This mobility rate decreased with increasing septal age and under stress conditions, likely reflecting a regulatory mechanism affecting septal pore diameter. Modeling revealed that such regulation offers effective control of compound concentration between compartments. Modeling also showed that the incidence of septal closure in A. niger had an even stronger impact on cytoplasmic continuity. Cytoplasm of hyphal compartments was shown not to be in physical contact when separated by more than 4 septa. Together, data show that apical compartments of growing hyphae behave unicellularly, while older compartments have a multicellular organization. IMPORTANCE The hyphae of higher fungi are compartmentalized by porous septa that enable cytosolic streaming. Therefore, it is believed that the mycelium shares cytoplasm. However, it is shown here that the septa of Aspergillus niger are always closed in the oldest part of the hyphae, and therefore, these compartments are physically isolated from each other. In contrast, only part of the septa is closed in the youngest part of the hyphae. Still, compartments in this hyphal part are physically isolated when separated by more than 4 septa. Even open septa act as a barrier for cytoplasmic mixing. The mobility rate through such septa reduces with increasing septal age and under stress conditions. Modeling shows that the septal pore width is set such that its regulation offers maximal control of compound concentration levels within the compartments. Together, we show for the first time that Aspergillus hyphae switch from a unicellular to multicellular organization.

scholarly journals Mycoflora of Cotton Plant (Gossypium hirsutum L.) - with three New Records of Deuteromycetes from Bangladesh

2015 ◽  
Vol 39 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Shamim Shamsi ◽  
Najmun Naher ◽  
Rumana Azad
Keyword(s):  
Aspergillus Niger ◽  
Gossypium Hirsutum ◽  
Cotton Plant ◽  
New Record ◽  
New Records ◽  
Gossypium Hirsutum L ◽  
Colletotrichum Dematium ◽  
Fusarium Sp ◽  
Academy Of Sciences ◽  
First Time

Fungal diseases of cotton plant (Gossypium hirsutum L.) were studied during July, 2011 to June, 2012. Eleven cotton varieties were selected for the experiment. Alternaria alternata (Fr.) Keissler, Aspergillus niger van Tiegh, Colletotrichum gossypii Sowthworth, Colletotrichum dematium (Pers.) Grove, Curvularia clavata Jain, Fusarium sp., Gleotrichum sp., Hyalopycnis sp., Pestalotiopsis guepinii (Desm.) Stay, and a sterile fungus were isolated from infected leaves of cotton varieties CB6 and CB11. Colletotrichum gossypii was isolated from both the varieties and C. dematium was exclusively isolated from CB6. Varieties CB6 and CB11 were more susceptible than other varieties. Cotton varieties CB1, CB2, and CB3 showed negligible infection on leaves and bolls. Colletotrichum gossypii and Hyalopycnis sp. are new record for Bangladesh. Moreover, association of C. dematium, Gleotrichum sp., P. guepinii and Hyalopycnis sp. with cotton is being reported first time. The three fungi Colletotrichum. gossypii, C. dematium and Hyalopycnis sp. are described and illustrated in this account as new Deuteromycetous records for Bangladesh.Journal of Bangladesh Academy of Sciences, Vol. 39, No. 2, 213-221, 2015

Transglycosylation reaction and raw starch hydrolysis by novel carbohydrolase fromLipomyces starkeyi

2003 ◽  
Vol 8 (2) ◽  
pp. 106-111 ◽  
Author(s):  
Jin Ha Lee ◽  
Sun Ok Lee ◽  
Gwang Ok Lee ◽  
Eun Seong Seo ◽  
Suk Sang Chang ◽  
...  
Keyword(s):  
Starch Hydrolysis ◽  
Raw Starch ◽  
Transglycosylation Reaction ◽  
Raw Starch Hydrolysis

Efficient production of citric acid in segmented fermentation using Aspergillus niger based on recycling of a pellet-dispersion strategy

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105003-105009
Author(s):  
Baoshi Wang ◽  
Jian Chen ◽  
Hua Li ◽  
Fuxin Sun ◽  
Youran Li ◽  
...  
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Batch Fermentation ◽  
Fermentation Process ◽  
Efficient Production

Segmentation recycling fermentation based on a pellet-dispersion strategy to reconstruct the traditional citric acid batch fermentation process is reported.

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