scholarly journals VARIASI VIRULENSI VIRUS TUNGRO BERSUMBER DARI INOKULUM DI DAERAH ENDEMIS TUNGRO DI INDONESIA

2015 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Nyoman Widiarta ◽  
Syahrir Pakki

Variations in virulence of tungro viruses from various inoculum sources in tungro endemic areas in Indonesia. Rice tungro disease is caused by virus which is effectively transferred by the green leafhopper. Reactions of resistant varieties to virus sources of inocula from 15 tungro endemic areas were employed as indicator of variations of virus virulence. The green leafhopper of Sukamandi’s population was used as the vector and allowed to transfer viruses  acquired from tungro’s infected plants from 15 tungro endemic areas to five groups of virus resistant varieties based on parent source of resistance using free choice screening box method. The results showed that the most resistant variety was group V1-Tukad Petanu, followed by V4-Tukad Unda, V2-Tukad Balian and V3-Bondoyudo. Based on resistance test result group variety of V1-Tukad Petanu is recommended for 15 provinces source of incula except for Sulawesi Utara. Group variety of V4-Tukad Unda is not recommended to plant in Yogyakarta and Banten provinces. Group V2-Tukad Balian is not recommended to plant in Bali, Sulawesi Utara, Banten and Kalimantan Selatan provinces. Group V3-Bondoyudo is not recommended to plant in Jawa Tengah, Yogyakarta, and Banten provinces. There were variations in virus virulence among sources of inocula. Six virulence variants were identified, i.e. 001 (Jawa Barat, Nusa Tenggara Barat, Sulawesi Selatan, Jawa Timur, Lampung, Sulawesi Barat, Sulawesi Tengah, Sulawesi Tenggara, Papua), 011 (Jawa Tengah), 021 (Bali, Kalimantan Selatan), 051 (Yogyakarta), 071 (Banten) and 121 (Sulawesi Utara).

2003 ◽  
Vol 3 (1) ◽  
pp. 28-39
Author(s):  
Aniceto Villar ◽  
◽  
Lorenza de Pedro

Insecticide-sprayed and unsprayed IR64, rice variety susceptible to tungro virus had very poor growth and very low yield compared to the intermediate and resistant varieties. As a result, higher net profit was observed on PSB Rc18 (resistant variety) during the dry season, while a negative profit per cropping was obtained on the sprayed and unsprayed susceptible variety. The study implied that cypermethrin is not effective in controlling rice tungro disease, thus the use of resistant varieties is more effective and profitable.


2013 ◽  
Vol 15 (2) ◽  
pp. 65
Author(s):  
I Nyoman Widiarta ◽  
Adolf Bastian ◽  
Syahrir Pakki

Green leafhopper (GLH), Nephotettix virescens, is the most efficient vector of rice tungro virus disease. The disease is endemic in some provinces of Indonesia and commonly con-trolled using resistant varieties. Resistance of rice varieties to tungro could be classified into resistance to a virus and a vector. The history of GLH resistant varieties adoption affected the GLH adaptation in an area. The study was conducted in the period of 2009-2011 to evaluate the resistance status of five GLH resistant rice variety groups (T0-T4) using survival and transmission test. The GLH populations were collected from 15 tungro endemic provinces in Indonesia. The GLH was then reared in the greenhouse before used for the test. The degree of resistance to tungro viruses was calculated by adding the value of survival (weight x score of survival rate) and virus transmission rate (weight x score of transmission rate). The weights for survival and transmission rate were set to 40 and 60, respectively. The results showed that the rank of resistant variety groups in decreasing order of resistance were T4, T1, T2 and T3. Five variations in GLH transmission efficiency were identified, i.e. 170, 070, 050, 030 and 010. GLH populations from Bali and West Nusa Tenggara were the most efficient vector for rice tungro virus. We concluded that there were diversities in the degree of resistance among GLH resistant varieties. Variation in virus transmission efficiency (biotype) among GLH populations collected from various tungro endemic areas closely related to the history of adoption of rice varieties.


2006 ◽  
Vol 6 (2) ◽  
pp. 92-99
Author(s):  
Burhanuddin Burhanuddin ◽  
I Nyoman Widiarta

Refining integrated rice tungro disease management based on escaped strategy and varieties rotation. Rice tungro virus transmitted mainly by green leafhopper, N. virescens, infected rice in central rice  production in Indonesia. Tungro epidemic in South Sulawesi were succesfully controlled by integrating appropriate planting time and rotation of green leafhopper resistant varieties since 1973. Tungro was reported in 1998 after climate anomaly, caused by El-Nino and La-Nina. Pattern of green leafhopper population fluctuation and tungro disease incidence and green leafhopper adaptation to green leafhopper resistance variety were evaluated. The results showed that the fluctuation pattern of green leafhopper population in Lanrang which represents of areas  in Eastern Coarse of South Sulawesi  has changed. Green leafhopper colonies from endemic area in South Sulawesi were almost all well adapted to 4 groups of green leafhopper resistance varieties. Therefore it was suggested to adjust planting date and recommended to plant tungro virus resistance variety instead of green leafhopper resistance varieties.


2018 ◽  
Vol 21 (1) ◽  
pp. 91-100 ◽  
Author(s):  
MT Khatun ◽  
MA Latif ◽  
MM Rahman ◽  
M Hossain ◽  
TH Ansari ◽  
...  

Rice tungro is the most destructive and widespread among virus diseases found in almost all rice growing areas. In susceptible varieties, it causes 100% yield loss under favourable environmental conditions. Control of tungro disease by chemical applications is not effective and eco-friendly. Development of resistant variety against tungro is also difficult, because it is necessary to develop resistance either to the insect vector or to the virus or to the both. Identification of varieties with recovering ability will facilitate farmers to cultivate rice in tungro endemic areas. Bangladesh Rice Research Institute released six upland and eight rainfed lowland rice varieties, which were tested to evaluate their recovering ability against tungro disease. The test varieties including susceptible and resistant checks were artificially inoculated by viruliferous vector (Green leafhopper, Nephotettix virescens) of ten days after seeding in net house condition. Among the tested varieties BR26, BRRI dhan33 and BRRI dhan40 were the most susceptible and showed high infection rate against tungro disease. BRRI dhan37 and BR8 showed the highest recovering ability in rainfed lowland and upland rice, respectively, with the lowest yield reduction compared to the resistant check Kumragoir, which could be used in tungro endemic areas. From these investigations, all the tested varieties expressed distinct tungro disease symptoms after three weeks of inoculation. With the advancement of plant growth, varieties BR8 and BRRI dhan37 recovered from tungro syndrome due to their genetic makeup consisting tolerance potentiality showed better yield, while other varieties like BRRI dhan33 and BRRI dhan40 did not produce any grain yieldBangladesh Rice j. 2017, 21(1): 91-100


2013 ◽  
Vol 15 (2) ◽  
pp. 65
Author(s):  
I Nyoman Widiarta ◽  
Adolf Bastian ◽  
Syahrir Pakki

Green leafhopper (GLH), Nephotettix virescens, is the most efficient vector of rice tungro virus disease. The disease is endemic in some provinces of Indonesia and commonly con-trolled using resistant varieties. Resistance of rice varieties to tungro could be classified into resistance to a virus and a vector. The history of GLH resistant varieties adoption affected the GLH adaptation in an area. The study was conducted in the period of 2009-2011 to evaluate the resistance status of five GLH resistant rice variety groups (T0-T4) using survival and transmission test. The GLH populations were collected from 15 tungro endemic provinces in Indonesia. The GLH was then reared in the greenhouse before used for the test. The degree of resistance to tungro viruses was calculated by adding the value of survival (weight x score of survival rate) and virus transmission rate (weight x score of transmission rate). The weights for survival and transmission rate were set to 40 and 60, respectively. The results showed that the rank of resistant variety groups in decreasing order of resistance were T4, T1, T2 and T3. Five variations in GLH transmission efficiency were identified, i.e. 170, 070, 050, 030 and 010. GLH populations from Bali and West Nusa Tenggara were the most efficient vector for rice tungro virus. We concluded that there were diversities in the degree of resistance among GLH resistant varieties. Variation in virus transmission efficiency (biotype) among GLH populations collected from various tungro endemic areas closely related to the history of adoption of rice varieties.


1987 ◽  
Vol 109 (3) ◽  
pp. 609-610 ◽  
Author(s):  
G. S. V. Prasad ◽  
M. V. S. Sastry ◽  
J. R. K. Rao ◽  
A. Ghosh ◽  
Y. Kondala Rao

Brown planthopper, Nilaparvata lugens (Stal), is one of the most serious insect pests of rice (Oryza sativa L.) throughout Asia. Introduction of resistant varieties could be an effective means of minimizing losses from the pest. Several hundred rice cultivars resistant to the pest have been identified and the genetics of resistance has been analysed. Studies have shown that the genes for resistance to brown planthopper, bph-4, and to green leafhopper, Glh-3, are linked (Sidhu & Khush, 1979). Ideka & Kaneda (1983) reported that bph-2 for brown planthopper resistance was linked with the gene d2 for dwarfness. The present study sought to ascertain relationships between resistance to brown planthopper and tungro virus and grain characteristics.


2004 ◽  
Vol 18 (2) ◽  
pp. 315-324 ◽  
Author(s):  
Leanna L. Lyon ◽  
J. Wayne Keeling ◽  
Peter A. Dotray

Field experiments were conducted in 1999 and 2000 to evaluate and adapt the Herbicide Application Decision Support System (HADSS®) program for Texas Southern High Plains cotton production. Weed management systems (in glyphosate-resistant, bromoxynil-resistant, and nontransgenic cotton varieties) included trifluralin preplant incorporated (PPI) followed by (fb) HADSS postemergence-topical (POST) recommendations (PPI fb POST HADSS), HADSS recommendations alone (POST HADSS), and Texas Agricultural Experiment Station (TAES) recommendations for the Texas Southern High Plains. In both years, effective season-long weed control was achieved with all weed management systems in the glyphosate-resistant variety, but only the PPI fb POST HADSS and TAES weed management systems controlled Palmer amaranth and devil's-claw in the bromoxynil-resistant and nontransgenic varieties, compared with POST HADSS alone. No differences in cotton lint yield or net returns over weed control costs were observed with weed management systems across variety in 1999; however, in general, the glyphosate-resistant and nontransgenic varieties produced higher yields and net returns than the bromoxynil-resistant variety. In 2000, plots from the TAES weed management system produced higher lint yields than the plots of PPI fb POST HADSS recommendations in the glyphosate- and bromoxynil-resistant varieties, but plots of all management systems yielded similarly in the nontransgenic variety. In 2000, plots from the TAES system produced the highest net returns in the glyphosate- and bromoxynil-resistant varieties. In the nontransgenic variety, the PPI fb POST HADSS and TAES weed management systems produced higher net returns over weed control costs than the POST HADSS system.


2016 ◽  
Vol 20 (2) ◽  
pp. 65 ◽  
Author(s):  
Dini Yuliani ◽  
Rina H. Wening ◽  
NFN Sudir

<p>Seleksi Ketahanan Aksesi Plasma Nutfah Padi terhadap Hawar Daun Bakteri. Dini Yuliani, Rina H. Wening, dan Sudir. Usaha budi daya tanaman padi di Indonesia selalu dihadapkan pada berbagai kendala, di antaranya serangan penyakit hawar daun bakteri (HDB) yang disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo). Pengendalian penyakit HDB dengan varietas tahan merupakan salah satu teknik yang murah dan mudah dilakukan oleh petani padi. Aksesi plasma nutfah sebagai sumber tetua untuk perakitan varietas tahan perlu diketahui reaksi ketahanannya terhadap penyakit HDB. Penelitian ini bertujuan untuk menyeleksi ketahanan aksesi plasma nutfah terhadap penyakit HDB patotipe III, IV, dan VIII. Penelitian dilaksanakan di Kebun Percobaan Balai Besar Penelitian Tanaman Padi di Sukamandi, Subang, Jawa Barat pada Musim Hujan 2012/2013 dan Musim Kemarau 2013 dengan Rancangan Acak Terpisah. Petak utama adalah tiga patotipe Xoo, yaitu patotipe III, IV, dan VIII. Sedangkan anak petak adalah materi yang diuji, yaitu 20 aksesi plasma nutfah dan tiga varietas pembanding. Sebanyak 20 rumpun tanaman per petak diinokulasi Xoo dengan metode pengguntingan. Inokulasi dilakukan pada saat pertanaman menjelang stadium primordia. Ujung-ujung daun digunting sepanjang kira-kira 10 cm dari ujung daun dengan gunting inokulasi yang berisi suspensi bakteri Xoo umur 48 jam dengan kepekatan 108 cfu. Pengamatan keparahan penyakit HDB dilakukan dengan mengukur panjang gejala terpanjang pada umur dua, tiga, dan empat minggu setelah inokulasi. Hasil pengujian ketahanan terhadap HDB patotipe III, IV, dan VIII diperoleh tiga aksesi plasma nutfah yang berasal dari galur isogenik menunjukkan keparahan penyakit HDB tidak berbeda nyata dengan varietas pembanding tahan Angke pada dua musim tanam. Galur isogenik tersebut, yaitu IRBB 55, IRBB 60, dan IRBB 61. Ketiga galur isogenik tersebut dapat dijadikan tetua tahan dalam perakitan varietas unggul baru tahan HDB.</p><p> </p><p><strong>Abstrak</strong></p><p>Rice cultivation in Indonesia has been faced with many obstacles, including the attack of bacterial leaf blight (BLB) that caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo). Resistant variety was considered as the cheap technique to control BLB disease and could be used by rice farmers. Germplasm accessions as a source to build resistant varieties must be known their resistance to BLB disease. This study aimed at selecting the resistance germplasm accessions to BLB pathotype III, IV, and VIII. The study was conducted at the Experimental Station of the Indonesian Center for Rice Research in Sukamandi, Subang, West Java at wet season 2012/2013 and dry season 2013 with Split Plot Design. The main plot was three pathotypes Xoo i.e. pathotype III, IV, and VIII. The subplot was 20 germplasm accessions and three check varieties. A total of 20 hills of rice plants per plot were inoculated by Xoo with cutting method. Inoculation was conducted before the primordia stage. Inoculation of bacterial suspension containing Xoo aged 48 hours at a concentration of 108 cfu. Observations BLB disease severity was done by measuring the longest length of symptoms at the age of two, three, and four weeks after inoculation (WAI). The result showed that three germplasm accessions from near isogenic lines were not significantly different with the check varieties (Angke) in their resistance to Xoo pathotype III, IV, and VIII in two cropping seasons. The isogenic lines were IRBB 55, IRBB 60, and IRBB 61 can be used in the assembly of resistant new varieties to BLB.</p>


Author(s):  
K. Deepa ◽  
M. Ariavanamkatha Pillai ◽  
N. Murugesan

Host plant resistance has been a valuable tool in the management of planthoppers. The experiment was carried out to assess the level of total phenols, Ortho-dihydroxy phenols, Phenols, crude silica and total sugars in BPH (Brown Plant Hopper) affected rice leaves. Resistant varieties showed high amount of phenols, O.D. Phenols and crude silica as against low quantity of total sugars. These, biochemical basis of resistant method is also identifying the resistant variety against brown planthopper (<italic>Nilaparvata lugens</italic>) in rice.


1984 ◽  
Vol 62 (5) ◽  
pp. 1036-1043 ◽  
Author(s):  
Michel Bounias ◽  
Claude Coulomb ◽  
Philippe J. Coulomb

The chromatographic analysis of the free carbohydrate content of leaves and stems of pepper showed 19 major fractions which were gathered in 8 structural classes. A number of correlations were found between the respective carbohydrate levels of two varieties of pepper, one susceptible and the other resistant to downy mildew, and also between those of healthy and inoculated tissues. Some cases of departures from the established correlations were observed and discussed with respect to their hypothetical involvement in the character of resistance and susceptibility to the infection. The concentrations of free carbohydrates of the dideoxyhexose group are increased in inoculated leaves of both susceptible and resistant varieties and those of the rhamnose group are depressed in inoculated leaves of the sole susceptible variety. Sucrose strongly increases in inoculated stems but decreases in the inoculated leaves of the sole resistant variety; concomitantly glucose and fructose also increase in inoculated stems, whereas they decrease in inoculated leaves of both susceptible and resistant strains. The results suggest a number of hypotheses concerning the molecular mechanisms of the host resistance to infection in relation to the cellular pathways of sugar translocation.


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