KEANEKARAGAMAN DAN KEKERABATAN LALAT BUAH (DIPTERA: TEPHRITIDAE) DI KALIMANTAN SELATAN BERDASARKAN KARAKTER MORFOLOGI DAN MOLEKULAR (RAPD-PCR DAN SEKUENSING DNA)

Diversity and phylogeny of fruit fly (Diptera: Tephritidae) in South Kalimantan based on morphology and molecular (RAPD-PCR and DNA sequencing). Seven species of fruit fly was known by morphological identification. The fruit flies were found from trapping with methyl eugenol and fruit collecting at all study sites in South Kalimantan. The results showed that as much as 17 plants were infected by fruit fly. Dendrogram based on morphological identification analyzed by using UPGMA with MEGA 4 program consisted in a group consisting of 5 sub-groups. Bactrocera carambolae and Bactrocera papayae of morphology were still a closely related fruit fly at 0.935. Whereas, based on RAPD result analized by UPGMA using 20 character of DNA based, showed that out of seven species consisted 2 groups, 1st group were B. umbrosa, B. occipitalis and sub-group of B. latifrons. The second group consists of sub-groups B.carambolae, B. papaya, sub-group B. albistrigata and B. cucurbitae. The results of dendrogram from sequencing DNA fruit fly analysis comprised one of group and three sub-groups. The first sub-groups were B. papayae, B. carambolae, B. occipitalis, B.latifrons. The second subgroup were B. cucurbitae and B. umbrosa. While B. albistrigata separate but still one group with another fruit flies. The results of DNA sequencing showed that there were a homology of the seven species of the fruit fly i.e at 83 base pair / bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A ). The length of the base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons were respectively 615, 898, 570.969, 898 and 615 bp. The results of morphological analysis and RAPD methods showed difference in the distribution of groups and sub-groups. But based on morphologycal and DNA identification seven species of fruit flies found were all same as the genebank.

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THE DIVERSITY Of FRUIT FLIES IN RED GUAVA CROPS AND PARASITOID PARASITIZATION LEVEL IN DISTRICT DELI SERDANG

Journal of Community Research and Service ◽

10.24114/jcrs.v1i2.9331 ◽

2018 ◽

Vol 1 (2) ◽

pp. 26

Author(s):

Putri Mustika Sari ◽

Darma Bakti ◽

Maryani Cyccu Tobing

Keyword(s):

Fruit Fly ◽

Fruit Flies ◽

Methyl Eugenol ◽

Morphological Identification ◽

Waste Processing ◽

Bactrocera Carambolae ◽

The World ◽

Important Pest

AbstractMorphological and Molecular of Identification Bactrocera sp. (Diptera: Tephritidae) in 5 Location of Red Guava Crops District Deli Serdang. Fruit flies are a concern in the world because they are an important pest in fruits. Seven species of fruit flies (Bactrocera carambolae, Bactrocera papayae, Bactrocera curcubitae, Bactrocera caudata, Bactrocera albistrigata, Bactrocera tau, Bactrocera umbrosa) have been identified from the morphological identification of fruit flies in LIPI. The fruit fly is obtained from trapping with methyl eugenol, cocoa waste processing, and a mixture of both. Found 2 species of parasitoid (Psytallia sp. near Walker; Psytallia sp. near Walkeri) from the rearing of fruit that was attacked at five locations of red guava plantation district Deli Serdang.Keywords : Bactrocera spp., morphology, atractant, parasitoid

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MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE

International Journal of Biosciences and Biotechnology ◽

10.24843/ijbb.2018.v06.i01.p03 ◽

2018 ◽

Vol 6 (1) ◽

pp. 34

Author(s):

I Putu Sudiarta ◽

Dwi Martiningsia ◽

I Nyoman Wijaya

Keyword(s):

Cytochrome Oxidase ◽

Molecular Identification ◽

Fruit Fly ◽

Fruit Flies ◽

Cytochrome Oxidase I ◽

Coi Gene ◽

Morphological Identification ◽

Mitochondrial Cytochrome ◽

Accurate Identification ◽

Mitochondrial Cytochrome Oxidase

Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.

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MORPHOLOGICAL IDENTIFICATION AND POPULATION OF FRUIT FLY (Bactrocera sp.) (Diptera: Tephritidae) IN CHILI FIELDS, SAVANAJAYA VILLAGE BURU DISTRICT

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/jhptt.220123-129 ◽

2020 ◽

Vol 20 (2) ◽

pp. 123-129

Author(s):

Jeffij V. Hasinu ◽

John A. Patty ◽

Gratiana N. C. Tuhumury

Keyword(s):

Fruit Fly ◽

Production Quality ◽

Fruit Flies ◽

Methyl Eugenol ◽

Morphological Identification ◽

Total Catch ◽

Cotton Ball ◽

Horticultural Crops

Morphological identification and population of fruit fly (Bactrocera sp.) (Diptera: Tephritidae) in chili fields, SavanajayaVillage Buru District. Fruit fly (Bactrocera sp.) are pests that damage horticultural crops, one of which is chili and has thepotential to reduce the production quality and quantity. The aim of this research was to identify and obtain populationnumbers of fruit fly species in the chili fields in Savanajaya Village, Buru District. This study used diagonal sampling methodwith fruit fly traps placed randomly at each sub location of sampling, traps were modified Steiner ’s Trap type. Each trap wasconsisted of 1.5 mL of methyl eugenol on cotton ball, the treatment was repeated three times. Observations were at 3, 6, 9 and12 days after application (daa). The identification results showed that the fruit flies species that trapped were Bactroceradorsalis (Hendel) and Bactrocera umbrosus (Fabricius). The highest number of fruit fly was trapped at 6 daa with an averageof 110.67 to 134.03 or 48.51 to 58.73% from total catch, in addition the lowest catch was at 12 daa with an average of 29.67 to64.67 or 12.99 to 28. 34%. The average number of trapped population was 914.62 individuals.

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Seasonal Abundance and Diversity of Fruit Flies (Diptera: Tephritidae) in Three Types of Plant Formations in Western Burkina Faso, West Africa

Annals of the Entomological Society of America ◽

10.1093/aesa/saaa004 ◽

2020 ◽

Vol 113 (5) ◽

pp. 343-354 ◽

Cited By ~ 1

Author(s):

Issaka Zida ◽

Souleymane Nacro ◽

Rémy Dabiré ◽

Irénée Somda

Keyword(s):

Burkina Faso ◽

Insect Pests ◽

Fruit Fly ◽

Fruit Flies ◽

Bactrocera Dorsalis ◽

Seasonal Abundance ◽

Mass Trapping ◽

Tephritid Species ◽

Study Sites ◽

Western Burkina Faso

Abstract Fruit flies are significant insect pests, worldwide. Tephritid species diversity and their seasonal abundance were investigated over 2 yr (May 2017 to May 2019) in Western Burkina Faso. A mass trapping experiment consisting of 288 Tephri Trap types, operating with four types of parapheromones comprising methyl eugenol, terpinyl acetate, trimedlure, and cue lure and an insecticide (Dichlorvos), was used for attracting and killing insects. Plant formations including natural fallows, mango orchards, and agroforestry parks in each of the six study sites were selected for data collection. Twenty-nine tephritid species belonging to 10 genera were identified. Fourteen fruit fly species were identified for the first time in Burkina Faso. The genera Ceratitis MacLeay (Diptera : Tephritidae) and Dacus Fabricius (Diptera: Tephritidae) with, respectively, 14 and 7 species recorded were the most represented. The dominant species caught was the invasive Bactrocera dorsalis Hendel (Diptera: Tephritidae) followed by Ceratitis cosyra Walker (Diptera: Tephritidae) and Ceratitis silvestrii Bezzi (Diptera: Tephritidae). The fruit fly population density was very high during the rainy season, with peaks occurring in June or July. The fruit fly species were generally more abundant during the hot and rainy seasons than during the cold and dry seasons. The highest diversity was recorded in natural fallows, as compared with the mango orchards and agroforestry parks. Tephritid species found refuge in the mango orchards during the dry and cold periods. The results of that investigation may be used for developing a sustainable pest management strategy for commercial orchards.

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APLIKASI MAT DALAM PENGENDALIAN LALAT BUAH Bactrocera sp. (DIPTERA : TEPHRITIDAE) PADA TANAMAN CABE

EUGENIA ◽

10.35791/eug.17.2.2011.3533 ◽

2011 ◽

Vol 17 (2) ◽

Author(s):

Maxi Lengkong ◽

Caroulus S. Rante ◽

Merlyn Meray

Keyword(s):

Field Research ◽

Fruit Fly ◽

Fruit Flies ◽

Methyl Eugenol ◽

Bactrocera Dorsalis ◽

Crop Acreage ◽

Bactrocera Carambolae ◽

Male Annihilation ◽

Bactrocera Philippinensis ◽

Male Annihilation Technique

ABSTRACT Research was aiming to determine the effectiveness of the attractant methyl eugenol + traps modification in the catch of species of fruit flies in the chilli plants at any given time and to identify the types of fruit flies trapped with methyl eugenol + traps modification on chilli crop acreage. The field research was conducted in the area of chilli crop in three places namely District Pineleng, Tompaso / Langowan and Modoinding. Observation number and type of fruit flies was performed at intervals of 2 weeks of observation for 3 months. The result showed that the number of fruit fly caught or trapped dead at 30 traps set in chilli crop acreage was 1278. The highest trapped was 164 whereas the lowest trapped was 89. The number of male fruit flies trapped was high implying that reduction of male fruit fly population in the field. There were five types of fruit flies identified from area of Pineleng, Tompaso and Modoinding namely Bactrocera umbrosa Fabricus, Bactrocera dorsalis (Hendel), Bactrocera philippinensis, Bactrocera carambolae, and Bactrocera sp. The five types were attracted to methyl eugenol. Key words : MAT (male annihilation technique), Bactrocera umbrosa Fabricus, B. dorsalis (Hendel), B. philippinensis, B. carambolae

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DINAMIKA POPULASI LALAT BUAH (Diptera: Tephritidae) PADA TANAMAN BUAH-BUAHAN DI KABUPATEN DHARMASRAY

Agrin ◽

10.20884/1.agrin.2017.21.1.339 ◽

2017 ◽

Vol 21 (1) ◽

Author(s):

Sri Heriza

Keyword(s):

Key Words ◽

Fruit Fly ◽

Fruit Flies ◽

Fruit Tree ◽

Bactrocera Cucurbitae ◽

Fruit Crops ◽

Control Fruit ◽

Bactrocera Carambolae ◽

West Sumatra ◽

Fruit Plants

Kegiatan penelitian Dinamika Populasi Lalat Buah (Diptera: Tephritidae) Pada Tanaman Buah-Buahan DiKabupaten Dharmasraya ini telah dilakukan mulai pada tanggal 1Agustus 2016 sampai dengan tanggal 10November 2016 di Kabupaten Dharmasraya. Kegiatan penelitian ini adalah sebuah penelitian untuk memperkayapengetahuan mengenai lalat buah yang meliputi kesamaan, kelimpahan, dominansi lalat buah pada tanaman buahbuahandi Dharmasraya, dan dapat membantu dalam upaya pengendalian hama lalat buah pada tanaman buahbuahanyang dibudidayakan. Data penelitian diambil dari mengumpulkan buah busuk yang ada di pasar KabupatenDharmasraya dan trapping di sentra buah-buahan di Kabupaten Dharmasraya, Propinsi Sumatera Barat kemudiandibawa ke Laboratorium Entomologi Prodi Agroekoteknologi Fakultas Pertanian Kampus III Universitas AndalasDharmasraya untuk diidentifikasi. Dari hasil penelitian ini terlihat keragaman spesies lalat buah di pasar dan disentra buah-buahan di Kabupaten Dharmasraya sangat rendah dan di dominansi oleh spesies Bactroceracarambolae dan Bactrocera papayae, dan ditemukan 6 spesies lalat buah yang ada di lokasi pasar dan sentra buahbuahan di Kabupaten Dharmasraya yaitu Bactrocera papayae Drew & Hancock, Bactrocera carambolae Drew &Hancock, Bactrocera umbrosa Fabricius, Bactrocera cucurbitae Coquillete, Bactrocera caudata Fabricius danBactrocera albistrigata de Maijere (Diptera:Tephritidae).Kata Kunci: populasi, lalat buah, tanaman buahABSTRACTResearch activities Fruit Fly Dynamics Population (Diptera: Tephritidae) On Fruit Plants In DharmasrayaDistrict has been done starting on August 1st ,2016 until November 10th, 2016 at Dharmasraya District. Thisresearch activity are research to enrich the knowledge of fruit flies that include similarity, abundance, dominanceof fruit flies in fruit crops in Dharmasraya, and can help in the effort to control fruit fly pests on fruit tree. Datawere taken from collected rotten fruit on the market Dharmasraya and trapping in the center of fruits inDharmasraya Regency, West Sumatra Province and then taken to the Laboratory of Entomology Faculty ofAgriculture Campus III University of Andalas Dharmasraya will be identified. From the results of this study looksdiversity of species of fruit flies in the market and at the central fruit in Dharmasraya very low and in dominanceby species Bactrocera carambolae and Bactrocera papayae, and found six fruit fly species that exist in the marketplace and center for fruits in the District Dharmasraya namely Bactrocera papayae Drew & Hancock, Bactroceracarambolae Drew & Hancock, Bactrocera umbrosa Fabricius, Bactrocera cucurbitae Coquillete, Bactroceracaudata Fabricius and Bactrocera albistrigata de Maijere (Diptera: Tephritidae).Key words: Population, Fruit Flies, Fruit Crops

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Pengaruh formulasi metil eugenol block plus terhadap tangkapan lalat buah Bactrocera spp. pada tanaman cabai

Jurnal Entomologi Indonesia ◽

10.5994/jei.18.2.93 ◽

2021 ◽

Vol 18 (2) ◽

pp. 93-101

Author(s):

Agus Susanto ◽

◽

Agus Dana Permana ◽

Sri Hartati ◽

Tohidin Tohidin ◽

...

Keyword(s):

Active Ingredient ◽

Block Design ◽

Fruit Fly ◽

Fruit Flies ◽

Methyl Eugenol ◽

Chili Pepper ◽

Bactrocera Dorsalis ◽

Bactrocera Carambolae ◽

Randomized Block Design ◽

Block Product

Fruit fly Bactrocera spp. is one of important pests in chili pepper. The use of methyl eugenol (ME) block plus as an attractant in traps is one method of controlling fruit flies populations. This study aims to find out the appropriate formulation of ME block plus formulation in increasing fruit fly catches on chili pepper. The experiment was carried out from January to April 2018 in Lembang Village, Lembang District, West Bandung Regency. The experiment used a randomized block design consists of eight treatments and three replications. The treatments tested were ME 2 ml was applied to all treatments using cotton; block; chili essence 4 ml block; 4 ml block of guava essence; orange essence 4 ml block; starfruit essence 4 ml block; mango essence 4 ml block; an attractant block product with the active ingredient ME. Although the results showed that all the treatments tested could not attract female fruit flies, the essence formulation was successful in attracting male fruit flies by 87%. The 4 ml chili essence in the 2 ml ME block formulation was the most effective treatment to increase male fruit fly catches by as much as 377 fruit flies/traps/week specifically the species Bactrocera dorsalis (Hendel), Bactrocera carambolae Drew & Hancock, Bactrocera umbrosa Fabricius, and hybrid fruit flies B. dorsalis x B. carambolae.

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Kelimpahan Spesies Lalat Buah (Diptera: Tephritidae) dan Parasitoidnya yang Berasosiasi pada Tanaman Belimbing (Averrhoa carambola L.) di Kabupaten Gianyar

Agrotrop : Journal on Agriculture Science ◽

10.24843/ajoas.2019.v09.i01.p01 ◽

2019 ◽

Vol 9 (1) ◽

pp. 1

Author(s):

I NYOMAN WIDNYANA PUTRA ◽

I WAYAN SUSILA ◽

I GUSTI NGURAH BAGUS

Keyword(s):

Fruit Fly ◽

Fruit Flies ◽

Parasitization Rate ◽

Averrhoa Carambola ◽

Fopius Arisanus ◽

Bactrocera Carambolae ◽

Star Fruit ◽

Fruit Plants

Abundance of Fruit Fly Species (Diptera: Tephritidae) and Its Parasitoid Associated on Star Fruit Plants (Averrhoa carambola L.) in Gianyar regency. This study was conducted to determine the abundance of fruit flies, and itsspecies of parasitoid on starfruit plants in Gianyar regency. Samples of star fruit attacked by fruit fly was taken purposively. The purpose of this study was to know the abundance, composition, and percentage of damage, kinds of parasitoid and parasitization rate. The results showed that was found 2 kinds of fruit flies, that are Bactrocera. carambolae and B. papayae. B. carambolae was dominant in all districts in Gianyar Regency compare themB. papayae. In Sukawati district was 64%, (492 adults), Blahbatuh district was 59.817% (460 adults), Ubud district was 61.528% (491 adults), Payangan district was 55.476% (390 adults), Tampaksiring district was 55.001% (390 adults), respectively. Meanwhile, B. papayae in Sukawati district was 35.348%, (269 adults), Blahbatuh district was 40.182% (309 adults), Ubud district was 38.471% (307 adults), Payangan district was 44.523% (313 adults) and Tampaksiring district was 40.998% (271 adults), respectively. The average of damage percentage was 86.33%. There are 3 kinds of parasitoids associated on fruit flies in starfruit plants in Gianyar regency. The parasitoid are Diachasmimorpha sp, Opius sp. and Fopius arisanus. The average parasitization rate was 11.12%, with the highest parasitization rate in Ubud district was 12.40% and the lowest in Tampaksiring districtwas 9.58%.

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Diversity of Fruit Flies (Diptera: Tephritidae) on Sweet Orange Plantations in Central Bangka Regency, Bangka Belitung Islands Province

AGROSAINSTEK Jurnal Ilmu dan Teknologi Pertanian ◽

10.33019/agrosainstek.v5i2.211 ◽

2021 ◽

Vol 5 (2) ◽

pp. 124-132

Author(s):

Herry Marta Saputra ◽

Budi Afriyansyah

Keyword(s):

Sweet Orange ◽

Fruit Fly ◽

Fruit Flies ◽

Methyl Eugenol ◽

Bactrocera Carambolae

Central Bangka Regency is one of central orange production in Bangka Belitung Islands Province. One of the important pests of sweet orange plants is fruit flies (Diptera: Tephritidae). This research aimed to study the diversity of fruit flies (Diptera: Tephritidae) on sweet orange plantations in Central Bangka Regency, Bangka Belitung Islands Province. The fruit flies were collected using Lynfield traps which installed in three villages producing sweet oranges in Central Bangka Regency. The types of attractants used to trap male fruit flies are methyl eugenol and cue lure. The fruit flies collected during the study were 4795 individuals, 3 genera, and 14 species. Fruit flies collected from the methyl eugenol were different compared to those collected using lure attractant trap. Most species (13 species) of fruit fly were found in Terentang village, and the rest was same (9 species). Bactrocera carambolae and B. dorsalis were the dominant fruit fly species in the three research locations.

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