scholarly journals Immunohistochemical study of trophoblast cells using abortion material in the gestation period 9-12 weeks of pregnancy in women against the background of inflammatory diseases of the female genital organs

2021 ◽  
Vol 25 (1(97)) ◽  
pp. 31-36
Author(s):  
A. Hoshovska ◽  
I. Davydenko ◽  
S. Yasnikovska ◽  
A. Tesliuk ◽  
O. Panchenko
Keyword(s):  
Gestational Age ◽  
Imaging System ◽  
Chorionic Villus ◽  
Gestation Period ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Chorionic Villi ◽  
Infectious Process ◽  
Torch Infection ◽  
Immunohistochemical Studies

Abstract. This article is devoted to the results of studies of trophoblast during the gestation of 9-12 weeks. This study is a fragment of a series of immunohistochemical studies of trophoblast with TORCH infection, which are scheduled to be carried out at different gestational dates. Purpose of the study – in various types of trophoblast using the immunohistochemical method to establish the features of the expression of metalloproteinase-2 using abortion material in gestation period 9-12 weeks of pregnancy with TORCH infection compared with observations without an infectious process. Material and methods. The main group of the study consisted of 24 observations of TORCH infection, and the control group - 22 observations of an aborted pregnancy without signs of an infectious process (abortion for social reasons). The study examined abortion material 9-12 weeks of gestation. The method of microdensitometry in a specialized computer program ImageJ evaluated the optical density of the color, and an immunohistochemical procedure was performed on metalloproteinase-2 with primary antibodies and a polymer antigen imaging system using DAKO diaminobenzidine. Results. According to the results of immunohistochemical studies using computer microdensitometry at a gestational age of 9-12 weeks, the very expression of metalloproteinase-2 is observed in the invasive trophoblast, the smallest - in the syncytotrophoblast of the choric villi, both with TORCH infection and without an infectious process, and intermediate values are noted in chorionic villus cytotrophoblast and cell column cytotrophoblast. Infection, the expression of metalloproteinase-2 is reduced in all four types of trophoblast (cytotrophoblast of chorionic villi; cytotrophoblast of cell columns; invasive cytotrophoblast in endometrial fragments), with TORCH except for syncytotrophoblast of chorionic villi. Conclusions. According to the results of immunohistochemical studies using computer microdensitometry at a gestational age of 9-12 weeks, both with TORCH infection and without an infectious process, the largest expression of metalloproteinase-2 is observed in invasive trophoblast, the smallest - in the syncytiotrophoblast of chorionic villi, and intermediate indices are noted in the cytotrophoblast of chorionic villi and the cytotrophoblast of cell columns. The expression of metalloproteinase-2 decreases in all types of trophoblast with TORCH infection, excluding the syncytiotrophoblast of chorionic villi.

scholarly journals INVESTIGATION OF TROPHOBLAST IN TORCH INFECTION ON ABORTION MATERIAL IN THE FIRST TRIMESTER OF PREGNANCY

2019 ◽  
Vol 18 (4) ◽  
pp. 58-62
Author(s):  
A. V. Goshovskaya ◽  
V. M. Goshovsky ◽  
S. M. Yasnikovska
Keyword(s):  
Gestational Age ◽  
Imaging System ◽  
Chorionic Villus ◽  
First Trimester ◽  
Control Group ◽  
Chorionic Villi ◽  
Infectious Process ◽  
First Trimester Of Pregnancy ◽  
Torch Infection ◽  
Immunohistochemical Studies

This study is a fragment of a series of immunohistochemical studies of trophoblast with TORCH infection, which are scheduled to be carried out at different gestational dates. This article deals with the results of trophoblast studies at the gestational age of 7-8 weeks. The study examined abortion material 7-8 weeks of gestation. The main group of the study consisted of 18 observations of TORCH infection, and the control group consisted of 17 observations of an aborted pregnancy without signs of an infectious process (abortion for social reasons). An immunohistochemical procedure was performed on metalloproteinases-2 with primary antibodies and polymer antigen imaging system using DAKO diaminobenzidine. The method of computer microdensitometry in a specialized computer program ImageJ evaluated the optical density of the color. According to the results of immunohistochemical studies using computer microdensitometry at the gestational age of 7-8 weeks, both with TORCH infection and without it an infectious process, strong expression of metalloproteinase-2 is observed in the invasive trophoblast, the smallest – in the syncytotrophoblast of the chorionic villi, and intermediate values are noted in chorionic villus cytotrophoblast and cell column cytotrophoblast. With TORCH infection the expression of metalloproteinase-2 decreases in all the four types of trophoblast (cytotrophoblast of chorionic villi; cytotrophoblast of cell columns; invasive cytotrophoblast in endometrial fragments), except for syncytotrophoblast of chorionic villi was found.

scholarly journals IMMUNOHISTOCHEMICAL STUDY OF METALLOPROTEINASES-2 IN THE TROPHOBLAST IN CASE OF TORCH-INFECTIONS ON THE MATERIAL OF ABORTIONS AT THE GESTATIONAL TERM OF 5-6 WEEKS

2016 ◽  
Vol 15 (4) ◽  
pp. 70-73
Author(s):  
A. V. Hoshovska ◽  
I. S. Davydenko ◽  
O. M. Davydenko ◽  
V. M. Hoshovskyi
Keyword(s):  
Computer Program ◽  
Gestational Age ◽  
Immunohistochemical Study ◽  
Polymer System ◽  
Immunohistochemical Method ◽  
Cytotrophoblast Cell ◽  
Infectious Process ◽  
Different Types ◽  
Immunohistochemical Studies ◽  
Torch Infections

This study is a fragment of a series of immunohistochemical studies of trophoblast in case of TORCH-infections, which are scheduled at different times of gestation. This article focuses on the results of studies of the trophoblast in gestational age 5-6 weeks. The aim of the study was by means of the immunohistochemical method to determine the features of expression of MP-2 in different types of trophoblast with TORCH-infections as compared to observations without an infectious process. Abortion material of 5-6 weeks of gestation was investigated. The main group included 16 observations of TORCH-infections, and the control - 14 observations of interrupted pregnancies with no signs of infection (abortion for social reasons). Immunohistochemical method was applied on metalloproteinases-2 primary antibody and polymer system for visualization of antigen using diaminobenzidine manufactured by DAKO. Using computer microdensitometry in a specialized computer program ImageJ the optical density of color was assessed. According to the results of immunohistochemical studies using the method of microdensitometry at the gestational age of 5-6 weeks, as in TORCH-infections and infectious process, the greatest expression of metalloproteinase-2 is noted in invasive trophoblast, the lowest - in the synthitiotrophoblast of chorial villi, and intermediate rates are seen in cytotrophoblast chorial villi and cell columns. With TORCH-infections, the expression of metalloproteinase-2 is reduced in all four types of trophoblast (cytotrophoblast chorial villi; cytotrophoblast cell columns; invasive cytotrophoblast in endometrial fragments), with the exception of synthiotrophoblast of chorial villi.

scholarly journals CD68+ M1 MACROPHAGES IS ASSOCIATED WITH PLACENTAL INSUFFICIENCY UNDER FETAL GROWTH RESTRICTION

2021 ◽  
Vol 74 (2) ◽  
pp. 213-219
Author(s):  
Varvara A. Berezhna ◽  
Tetiana V. Mamontova ◽  
Antonina M. Gromova
Keyword(s):  
Fetal Growth ◽  
Gestational Age ◽  
Fetal Growth Restriction ◽  
Preterm Births ◽  
Placental Insufficiency ◽  
Growth Restriction ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Stromal Component ◽  
Term Births

The aim: To elucidate the possible involvement of M1 and M2 macrophages in the placentas of women, whose pregnancies were complicated by fetal growth restriction (FGR) and resulted in term births after 37 weeks of gestation and preterm births up to 37 weeks of gestation. Materials and methods: CD68+ and CD163+ macrophages were studied by immunohistochemical method, placental morphology in the placentas of 16 women whose pregnancies were complicated by FGR and resulted in term births at a gestational age after 37 weeks (1-st group, n = 7) or resulted in preterm births at a gestational age up to 37 weeks (2-nd group, n = 9). The control group consisted of 10 placentas of women with physiological pregnancies and births. Results: Women 2-nd group showed significantly low weight of the placenta, a short gestation period at the time of delivery, and a prolonged labor period than women of the control group (p <0.001; p <0.001; p <0.05, respectively). The level of CD68+ and CD163+ macrophages in the placentas of women 2-nd group was significantly higher than in woman 1-st group (p <0.001, p <0.001, respectively). A significant correlation was found between the expression level of CD68+ monocytes in the intervillous space and the weight of a newborn (r = – 0.765; p = 0.016) in women 2-nd group. Conclusions: These studies suggest that in the placentas of women whose pregnancies were complicated by FGR and resulted in preterm births, the increased activation of CD68+ macrophages of the pro-inflammatory pool may be associated with disorders of the vascular and stromal component of the villous chorion with the development of involutive and dystrophic changes. In general, this fact probably determines the progress of chronic placental insufficiency and aggravates the development of fetal growth restriction.

Amnion Nodosum Revisited: Clinicopathologic and Placental Correlations

2007 ◽  
Vol 131 (12) ◽  
pp. 1829-1833
Author(s):  
Adebowale J. Adeniran ◽  
Jerzy Stanek
Keyword(s):  
Granular Material ◽  
Perinatal Mortality ◽  
Clinical Features ◽  
Gestational Age ◽  
Multiple Pregnancy ◽  
Control Group ◽  
Study Group ◽  
Chorionic Villi ◽  
Vascular Abnormalities

Abstract Amnion nodosum is commonly regarded as a placental hallmark of severe and prolonged oligohydramnios. It consists of nodules of amorphous granular material present on the surface of the amnion. We reviewed all 45 cases of amnion nodosum from our placental database from 1994 through 2003 (study group). The control group consisted of 45 cases from the same database matched for gestational age but without amnion nodosum. Oligohydramnios, multiple pregnancy, perinatal mortality, macerated stillbirths, and chronic twin-twin transfusion were the most common clinical features encountered in the study group. Luminal vascular abnormalities of chorionic villi, fibrosis of chorionic villi, and placental edema were the placental features that were more frequently present in the study group.

scholarly journals Features of the histological structure of trophoblast and chorionic villi with recurrent pregnancy loss in women with thrombophilias

2017 ◽  
Vol 25 (4) ◽  
pp. 621-641
Author(s):  
A. I. Mirov ◽  
O. N. Kharkevich ◽  
O. E. Golofast ◽  
I. B. Glukhovets
Keyword(s):  
Significant Role ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Chorionic Villus ◽  
Fetal Loss ◽  
First Trimester ◽  
Control Group ◽  
Histological Structure ◽  
Chorionic Villi ◽  
First Trimester Of Pregnancy

The frequency of recurrent pregnancy loss does not tend to decrease. This pathology continues to be one of the important problems of modern medicine. It is known that thrombophilia can play a significant role in the etiology of spontaneous reproductive losses. However, the pathogenesis of recurrent spontaneous loss of pregnancy in the presence of maternal thrombophilia is not fully understood. Aim. To identify the features of the histological structure of trophoblasts and chorionic villi in the first trimester of pregnancy in women with thrombophilia and recurrent pregnancy loss, with careful exclusion of other possible causes of fetal loss syndrome. Material and Methods. Histological examination of 49 chorion tissue samples from 24 patients with thrombophilia and recurrent pregnancy loss in the first trimester (study group) was performed. The controls were samples of chorion tissue taken during artificial abortion in 33 healthy women who had a history of 2 or more spontaneous labor without significant complications. Thrombophilia diagnosis and hemostasis system state evaluation was performed for all patients on the basis of analysis of 30 parameters according to standard methods. All studies were conducted at the Regional clinical hospital № 8 in Ryazan as well as the scientific and clinical center of hematology, oncology and immunology of the Ryazan State Medical University named after academician I.P. Pavlov of Health Ministry of the Russian Federation. Statistical processing of the obtained results was carried out with the help of computer program package Statistica (version 10). Results. Significant differences in the histological structure of trophoblast and chorionic villi in the studied women were revealed, in comparison with those in the control group. It is proved that the presence of thrombophilia negatively affects the process of embryogenesis and contributes to a significant reduction in the area of the chorionic villus vessels in the first trimester of pregnancy. Conclusion. It is proved that the presence of thrombophilia has a negative effect on the process of embryogenesis and significantly reduces the vascular area of chorionic villi that can probably play a significant role in the pathogenesis of recurrent pregnancy loss.

Perbandingan Efek Suplementasi Tablet Tambah Darah Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Pada Ibu Hamil Dengan Usiakehamilan 16-32 Minggu Di Desa Keniten Kecamatan Mojo Kabupaten Kediri

2017 ◽  
Vol 3 (1) ◽  
pp. 76
Author(s):  
Siti Asiyah ◽  
Dwi Estuning Rahayu ◽  
Wiranti Dwi Novita Isnaeni
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Gestational Age ◽  
Group Treatment ◽  
Sampling Technique ◽  
Hemoglobin Level ◽  
The Body ◽  
Control Group ◽  
Fetal Organ ◽  
And Control

The needed of Iron Tablet in pregnancy was increase than mother who not pregnant.  That  cause of  high metabolism at the pregnancy for formed of  fetal organ and energy. One of effort for prevent anemia in mother pregnant with giving the Iron tablet and vitamin c. The reason of  this research in 4 June – 11 July 2014 is for compare the effect of  iron tablet suplementation with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks In Desa Keniten Kecamatan Mojo Kabupaten Kediri. This research method using comparative analytical.  Research design type of Quasy Eksperiment that have treatment group and control group. Treatment group will giving by Iron tablet and 100 mg vitamin C, and control group just giving by iron tablet during 21 days. Population in this research are all of mother pregnant with Gestational Age Of 16-32 Weeks with Sampling technique is  cluster random sampling is 29 mother pregnant. Comparison analysis of  iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks, data analysis using Mann Whitney U-test and the calculated U value (44,5) less than U-table (51). So there was difference of iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks Therefore, the addition of vitamin C on iron intake is needed to increase the uptake of iron tablets. When the amount of iron uptake increases, the reserves of iron in the body will also increase, so as to prevent anemia in pregnant women; Keywords : Iron Tablet (Fe), Vitamin C, Hemoglobin level, Mother Pregnant

Serum kallistatin level is decreased in women with preeclampsia

2021 ◽  
Vol 49 (1) ◽  
pp. 60-66
Author(s):  
Onur Güralp ◽  
Nevin Tüten ◽  
Koray Gök ◽  
Kübra Hamzaoglu ◽  
Huri Bulut ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Diastolic Blood Pressure ◽  
Gestational Age ◽  
Late Onset ◽  
Serine Proteinase ◽  
Serum Levels ◽  
Control Group ◽  
Positive Correlation ◽  
Uncomplicated Pregnancy ◽  
Appropriate For Gestational Age

AbstractObjectivesTo evaluate the serum levels of the serine proteinase inhibitor kallistatin in women with preeclampsia (PE).MethodsThe clinical and laboratory parameters of 55 consecutive women with early-onset PE (EOPE) and 55 consecutive women with late-onset PE (LOPE) were compared with 110 consecutive gestational age (GA)-matched (±1 week) pregnant women with an uncomplicated pregnancy and an appropriate for gestational age fetus.ResultsMean serum kallistatin was significantly lower in women with PE compared to the GA-matched-controls (27.74±8.29 ng/mL vs. 37.86±20.64 ng/mL, p<0.001); in women with EOPE compared to that of women in the control group GA-matched for EOPE (24.85±6.65 ng/mL vs. 33.37±17.46 ng/mL, p=0.002); and in women with LOPE compared to that of women in the control group GA-matched for LOPE (30.87±8.81 ng/mL vs. 42.25±22.67 ng/mL, p=0.002). Mean serum kallistatin was significantly lower in women with EOPE compared to LOPE (24.85±6.65 ng/mL vs. 30.87±8.81 ng/mL, p<0.001). Serum kallistatin had negative correlations with systolic and diastolic blood pressure, creatinine, and positive correlation with GA at sampling and GA at birth.ConclusionsSerum kallistatin levels are decreased in preeclamptic pregnancies compared to the GA-matched-controls. This decrease was also significant in women with EOPE compared to LOPE. Serum kallistatin had negative correlation with systolic and diastolic blood pressure, creatinine and positive correlation with GA at sampling and GA at birth.

scholarly journals Evaluation of prenatal changes in fetal cardiac morphology and function in maternal diabetes mellitus using a novel fetal speckle-tracking analysis: a prospective cohort study

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Dong Wang ◽  
Caixia Liu ◽  
Xinyu Liu ◽  
Ying Zhang ◽  
Yu Wang
Keyword(s):  
Diabetes Mellitus ◽  
Cohort Study ◽  
Pregnant Women ◽  
Gestational Age ◽  
Speckle Tracking ◽  
Control Group ◽  
Second Trimester ◽  
Cardiac Morphology ◽  
Sphericity Index ◽  
And Function

Abstract Background Due to metabolic changes in the second trimester and the increasing number of pregnant women with obesity and advanced maternal age, the incidence of gestational diabetes mellitus (GDM) remains high. This study aimed to evaluate the effects of GDM on fetal cardiac morphology and function, and to determine whether these changes increase with increasing estimated fetal weight (EFW). Methods Fifty-eight women with GDM (GDM group) and 58 women with a healthy pregnancy (control group) were included in this prospective observational cohort study. Each group included subgroups of 31 pregnant women with a gestational age between 24+0 weeks and 27+6 weeks as well as 27 pregnant women with a gestational age between 28+0 weeks and 40+0 weeks. For all fetuses, a cine of 2–3 s in the four-chamber view was obtained, and online speckle-tracking analysis was performed using the GE Automatic Fetal Heart Assessment Tool (fetal HQ; General Electric Healthcare Ultrasound, Zipf, Austria) to measure the global sphericity index (GSI), global longitudinal strain (GLS), fractional area change (FAC), 24-segment sphericity index (SI), and 24-segment end-diastolic diameter of the left ventricle (LV) and right ventricle (RV). Data were analyzed using the independent t-test and Wilcoxon rank-sum test, as applicable. Results The GDM group (mean HbA1c value was 5.3 ± 0.57 mmol/L) showed a lower GSI value than the control group (1.21 vs. 1.27, P = 0.000), which indicated a rounder shape of the heart. In addition, fetuses in the GDM group demonstrated significant impairment in cardiac function compared to those in the control group (LV-GLS: -18.26% vs. -22.70%, RV-GLS: -18.52% vs. -22.74%, LV-FAC: 35.30% vs. 42.36%, RV-FAC: 30.89% vs. 36.80%; P = 0.000 for all). Subgroup analyses according to gestational age (24+0–27+6 weeks and 28+0–40+0 weeks) showed that the statistical differences were retained between the GDM and control groups in each subgroup. Conclusions Fetuses of women with GDM present with signs of biventricular systolic dysfunction according to deformation analysis using fetal HQ. Additionally, the heart had a rounder shape in the GDM group than in the control group. This study showed that fetal HQ can be used to assess fetal cardiac morphology and function easily and quickly, and the effects of GDM on fetal cardiac morphology and function appeared from the second trimester. Thus, whether earlier and stricter clinical intervention was necessary remained to be further studied. Furthermore, future studies will need to supplement the effects of blood glucose levels on GLS, FAC, GSI, and 24-segment SI. Additionally, the long-term follow-up after birth should also be improved to observe the influence of changes in the indicators on the prognosis.

scholarly journals The pathology of natural and experimentally induced Campylobacter jejuni abortion in sheep

2021 ◽  
pp. 104063872110332
Author(s):  
Michael J. Yaeger ◽  
Orhan Sahin ◽  
Paul J. Plummer ◽  
Zuowei Wu ◽  
Judith A. Stasko ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Chorionic Villus ◽  
Chorionic Villi ◽  
Purulent Meningitis ◽  
Microscopic Lesion ◽  
Transmission Electron ◽  
Induced Abortions ◽  
Gross Lesions ◽  
Hepatic Lesions ◽  
Experimentally Induced

We describe here the gross and microscopic lesions in 18 experimentally induced and 120 natural Campylobacter abortions. In natural Campylobacter abortions, gross lesions were reported infrequently; placentitis was recorded in 6% and hepatic lesions in 4% of our field cases. Placentitis was the microscopic lesion identified most consistently in natural abortions (93%) and was often observed in association with abundant bacterial colonies in chorionic villi (54%) and less often with placental vasculitis (13%). In natural abortions, suppurative fetal pneumonia (48%), necrosuppurative hepatitis (16%), and purulent meningitis (7%) were also observed. The better-preserved specimens from experimentally induced abortions were utilized to define placental changes more precisely. Placentitis was identified in all 18 experimentally induced abortions and was observed most consistently in the chorionic villus stroma (100%), often accompanied by suppurative surface exudate (89%). An inflammatory infiltrate was less commonly identified in the cotyledonary hilus (39%) and intercotyledonary placenta (22%). Bacteria were visualized in H&E-stained sections in 89% of placentas from experimentally infected ewes, primarily as well-demarcated bacterial colonies within subtrophoblastic, sinusoidal capillaries (89%), in the cotyledonary villus stroma (89%), and within the cytoplasm of trophoblasts (22%). Transmission electron microscopy and immunohistochemistry confirmed that the vast majority of the well-demarcated bacterial colonies characteristic of Campylobacter abortion were within subtrophoblastic sinusoidal capillaries. The most characteristic microscopic lesions identified in cases of Campylobacter abortion in sheep were placentitis with placental bacterial colonies, placental vasculitis, and fetal pneumonia.

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