scholarly journals Uji Pendahuluan Efek Fungisida Bunga Kembang Telang (Clitoria ternatea L.) terhadap Jamur Fusarium oxysporum f.sp. cepae Penyebab Penyakit Moler pada Bawang Merah

Agrikultura ◽  
2017 ◽  
Vol 28 (3) ◽  
Author(s):  
Tarkus Suganda ◽  
Satryo Restu Adhi

ABSTRACT Preliminary study on the fungicidal effect of butterfly pea flower (Clitoria ternatea L.) against Fusarium oxysporum f.sp. cepae, the causal agent of moler diseases on shallot Butterfly pea (Clitoria ternatea) has been known worldwide to possess numerous anti microbe substances, both against bacteria, fungi, nematodes, and insects. Researches were mostly conducted against human microbial pathogens, whereas only a few against plant pathogens, especially in Indonesia. A preliminary study has been carried out to reveal the fungicidal effects of flower extract in inhibiting the in-vitro growth of Fusarium oxysporum f.sp. cepae (FOC), the causal agent of moler diseases, one of the most destructive diseases on shallot. Results showed that the boiling extract of flower of butterfly pea at 5% concentration inhibited 46% the growth of FOC over control. Key words: Fungicidal effect, Cliteria ternatea, Fusarium oxysporum f.sp. cepae, In-vitro ABSTRAK Kembang telang (Clitoria ternatea) sudah lama dikenal di seluruh dunia mengandung berbagai senyawa antimikroba, baik terhadap bakteri, jamur, nematoda. bahkan insekta. Target utama patogen yang banyak diteliti adalah terhadap patogen penyakit pada manusia. Penelitian terhadap patogen tanaman, terutama di Indonesia masih sangat kurang. Uji pendahuluan sudah dilakukan untuk mengetahui efek fungisidal bunga kembang telang dalam menghambat pertumbuhan in-vitro jamur Fusarium oxysporum f.sp. cepae (FOC), penyebab penyakit moler yang sangat merugikan pada tanaman bawan merah. Hasil pengujian menunjukkan bahwa air rebusan bunga kembang telang memiliki kemampuan dalam menghambat pertumbuhan biakan FOC. Pada konsentrasi 5%, efek penghambatannya mencapai 46% terhadap kontrol. Kata kunci: Efek fungisida, Cliteria ternatea, Fusarium oxysporum f.sp. cepae, In-vitro

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4539
Author(s):  
Nguyen Minh Thuy ◽  
Vo Minh ◽  
Tran Ben ◽  
My Tuyen Thi Nguyen ◽  
Ho Ha ◽  
...  

Butterfly pea flower have great sensory attraction, but they have not yet been used widely in Vietnam. Extracts of butterfly pea flowers can be used conveniently as a natural blue colorant for food products. In this study, the identification of anthocyanin compounds in butterfly pea flowers was performed by UPLC coupled with a UV and Mass spectrometer instrument. Positive and negative ion electrospray MS/MS chromatograms and spectra of the anthocyanin compounds were determined. By analyzing the chromatograms and spectra for each ion, five anthocyanins were identified in the butterfly pea flower extract; these were delphinidin-3-(6”‐p-coumaroyl)-rutinoside, cyanidin 3-(6”-p-coumaroyl)-rutinoside, delphinidin-3-(p-coumaroyl) glucose in both cis- and trans- isomers, cyanidin-3-(p-coumaroyl-glucoside) and delphinidin-3-pyranoside. Additionally, based on their intensity, it was determined that cyanidin-3-(p-coumaroyl-glucoside) was the most abundant anthocyanin, followed by cyanidin 3-(6”-p-coumaroyl)-rutinoside, delphinidin-3-(p-coumaroyl-glucoside), delphinidin-3-(6”-p-coumaroyl)-rutinoside and delphinidin-3-pyranoside. In this study, cyanidin derivatives were discovered in butterfly pea flower extract, where these compounds had not been detected in previous studies.


2019 ◽  
Vol 295 ◽  
pp. 646-652 ◽  
Author(s):  
Charoonsri Chusak ◽  
Jowynn Ang Yu Ying ◽  
Joseph Lim Zhien ◽  
Porntip Pasukamonset ◽  
Christiani Jeyakumar Henry ◽  
...  

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 45
Author(s):  
Andrea Angarita-Rodríguez ◽  
Diego Quiroga ◽  
Ericsson Coy-Barrera

There is a continuous search for more reliable and effective alternatives to control phytopathogens through different strategies. In this context, indole-containing phytoalexins are stimuli-induced compounds implicated in plant defense against plant pathogens. However, phytoalexins’ efficacy have been limited by fungal detoxifying mechanisms, thus, the research on bioisosteres-based analogs can be a friendly alternative regarding the control of Fusarium phytopathogens, but there are currently few studies on it. Thus, as part of our research on antifungal agents, a set of 21 synthetic indole-containing phytoalexin analogs were evaluated as inhibitors against the phyopathogen Fusarium oxysporum. Results indicated that analogs of the N,N-dialkylthiourea, N,S-dialkyldithiocarbamate and substituted-1,3-thiazolidin-5-one groups exhibited the best docking scores and interaction profiles within the active site of Fusarium spp. enzymes. Vina scores exhibited correlation with experimental mycelial growth inhibition using supervised statistics, and this antifungal dataset correlated with molecular interaction fields after CoMFA. Compound 24 (tert-butyl (((3-oxo-1,3-diphenylpropyl)thio)carbonothioyl)-l-tryptophanate), a very active analog against F. oxysporum, exhibited the best interaction with lanosterol 14α-demethylase according to molecular docking, molecular dynamics and molecular mechanic/poisson-boltzmann surface area (MM/PBSA) binding energy performance. After data analyses, information on mycelial growth inhibitors, structural requirements and putative enzyme targets may be used in further antifungal development based on phytoalexin analogs for controlling phytopathogens.


Foods ◽  
2018 ◽  
Vol 7 (7) ◽  
pp. 102 ◽  
Author(s):  
Charoonsri Chusak ◽  
Christiani Henry ◽  
Praew Chantarasinlapin ◽  
Varanya Techasukthavorn ◽  
Sirichai Adisakwattana

2012 ◽  
Vol 59 (2) ◽  
pp. 51-58 ◽  
Author(s):  
Alicja Saniewska ◽  
Anna Jarecka ◽  
Zbigniew Biały ◽  
Marian Jurzysta

Antifungal activity of total saponins originated from roots of <i>Medicago hybrida</i> (Pourret) Trautv. were evaluated <i>in vitro</i> against six pathogenic fungi and eight individual major saponin glycosides were tested against one of the most susceptible fungi. The total saponins showed fungitoxic effect at all investigated concentrations (0.01%, 0.05% and 0.1%) but their potency was different for individual fungi. The highest saponin concentration (0.1%) was the most effective and the inhibition of <i>Fusarium oxysporum</i> f. sp. <i>callistephi</i>, <i>Botrytis cinerea</i>, <i>Botrytis tulipae</i>, <i>Phoma narcissi</i>, <i>Fusarium oxysporum</i> f. sp. <i>narcissi</i> was 84.4%, 69.9%, 68.6%, 57.2%, 55.0%, respectively. While <i>Fusarium oxysporum</i> Schlecht., a pathogen of <i>Muscari armeniacum</i>, was inhibited by 9.5% only. Eight major saponin glycosides isolated from the total saponins of <i>M. hybrida</i> roots were tested against the mycelium growth of <i>Botrytis tulipae</i>. The mycelium growth of the pathogen was greatly inhibited by hederagenin 3-O-<i>β</i>-D-glucopyranoside and medicagenic acid 3-O-<i>β</i>-D-glucopyranoside. Medicagenic acid 3-O-<i>β</i>-D-glucuronopyranosyl-28-O-<i>β</i>-D-glucopyranoside and oleanolic acid 3-O-[<i>β</i>-D-glucuronopyranosyl(1→2)-<i>α</i>-L-galactopyranosyl]-28-O-<i>β</i>-D-glucopyranoside showed low fungitoxic activity. Medicagenic acid 3-O-a-D-glucopyranosyl- 28-O-β-D-glucopyranoside, hederagenin 3-O-[α-L- hamnopyranosyl(1→2)-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]- 28-O-α-D-glucopyranoside and hederagenin 3-O-<i>β</i>-D-glucuronopyranosyl-28-O-<i>β</i>-D- lucopyranoside did not limit or only slightly inhibited growth of the tested pathogen. While 2<i>β</i>, 3<i>β</i>-dihydroxyolean-12 ene-23-al-28-oic acid 3-O-<i>β</i>-D-glucuronopyranosyl-28-O-<i>β</i>-D-glucopyranoside slightly stimulated mycelium growth of <i>B. tulipae</i>.


2020 ◽  
Vol 1 (2) ◽  
pp. 63-85
Author(s):  
Abdullah Muzi Marpaung

All part of the butterfly pea (Clitoria ternatea) plant reported having a various positive effect on human health. The blue petal, in particular, shows a wide range of functional activity including as an antioxidant, antidiabetic, antiobesity, anticancer, anti-inflammatory, and antibiotic. The hydrophilic phase of butterfly pea flower extract contains flavonol glycosides, anthocyanins, flavones, flavonols, phenolic acids, and cyclotides. Meanwhile, the terpenoids, alkaloids, and fatty acids were found in the lipophilic phase of butterfly pea flower extract. The proven health benefits and the wide range of the type of bioactive compounds promote butterfly pea flower as the source of functional food and nutraceuticals. However, a series of intensive research, including the clinical trial, is still needed.


2018 ◽  
Vol 17 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Nur Faezah Syahirah L ◽  
Muhammad Umar Lutfi MY ◽  
Atika A ◽  
Muhammad Hafiz R ◽  
Muhammad Zulhelmi OA ◽  
...  

Clitoria ternatea Linn (CT) or Butterfly pea flower is a blue flower edible plant, which is commonly used as food colouring. The objective of this study was to develop a liquid and paper pH indicator from CT extracts. The effectiveness of CT extract as pH indicator was evaluated by titration and product testing result against standard indicator. The stability of CT extracts were also conducted in 7 days storage. Findings from this study shows that CT extract is an effective liquid pH indicator except for weak acid samples. Whereas pH paper made from CT extract is effective as pH paper except for testing pH range from 4 -6. It is concluded thatCT extracts can be potentially effective pH indicator.Dhaka Univ. J. Pharm. Sci. 17(1): 97-103, 2018 (June)


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