scholarly journals Effect of Administration of Combination of Captopril and Celery Extract on Blood Pressure and Electrolyte Levels of Hypertensive Rats

2020 ◽  
Vol 7 (3) ◽  
pp. 81
Author(s):  
Siska Siska ◽  
Franciscus D. Suyatna ◽  
Abdul Mun'im ◽  
Anton Bahtiar
Keyword(s):  
Blood Pressure ◽  
Urine Volume ◽  
Concomitant Administration ◽  
Reduce Blood Pressure ◽  
Male Rats ◽  
Apium Graveolens ◽  
Hypertensive Rats ◽  
Treatment Groups ◽  
Urinary Potassium ◽  
Standard Value

Based on previous reports, the combination of captopril and celery could reduce blood pressure in hypertensive patients. This study aimed to investigate the changes of blood pressure, urine volume, sodium, and potassium level, due to concomitant administration of captopril with celery extract orally in male rats induced by 4% NaCl. The blood pressure was measured using a non-invasive tail method. The urine and blood were collected, and the sodium, potassium concentration, and cumulative urine volume were measured. The combination of 5 mg/kgBW of captopril and 40 mg/kgBW of celery extract decreased the blood pressure in hypertensive rats better than 5 mg/kgBW of captopril alone. The fell in blood pressure was followed by an increase in urine volume. Urinary and serum sodium, serum potassium levels tended to increase in all treatment groups, but not significantly different from the healthy group. Urinary potassium levels tended to decrease except in the combined 5 mg/kgBW of captopril and 40 mg/kgBW of celery extract. In conclusion, oral administration of a combination of 5 mg/kgBW captopril and 40 mg/kgBW celery extract decreased the blood pressure to the standard value in NaCl-induced hypertension rats.Keywords: Apium graveolens, captopril, celery, hypertension, pharmacodynamic

Effects of Nile Tilapia (Oreochromis niloticus) Viscera Hydrolyzate on Blood Pressure, TNF-α and IL-6 Expression in Rats (Rattus norvegicus) Induced by DOCA-Salt

2020 ◽  
Author(s):  
Putut Har Riyadi ◽  
Wendy Alexander Tanod ◽  
Dwi Titik Sulistiyati ◽  
Aulanni’am Aulanni’am ◽  
Eddy Suprayitno
Keyword(s):  
Blood Pressure ◽  
Reduce Blood Pressure ◽  
Positive Control ◽  
Hypertensive Rats ◽  
Fish Waste ◽  
Spontaneously Hypertensive ◽  
Treatment Groups ◽  
Tnf Α ◽  
Nile Tilapia Oreochromis Niloticus ◽  
The World

Background: Hypertension is a disease that has the potential to become the most significant cause of death in the world in 2020. Hypertension caused by an imbalance of the renin angiotensinogen system. Viscera hydrolyzate extract has an anti-hypertension activity. The research aimed to determine tilapia viscera hydrolyzate extract’s ability to reduce blood pressure, suppressing the expression of TNF-α and IL-6. Viscera hydrolyzed with alcalase and extracted to obtain viscera bioactive crude extract (VisBC).Methods: Spontaneously hypertensive rats induced by DOCA-salt, gave VisBC therapy. 15 Wistar rats divided into 5 treatment groups, namely normal control (without induced DOCA-salt), positive control (SHR-Spontaneous Hypertensive Rat = induced by DOCA-salt), comparative control (SHR + Captopril 5 mg/kg), VisBC 1 (SHR + BC 150 mg/kg) and VisBC 2 (SHR + BC 300 mg/kg), where each treatment consisted of 3 replications. Each treatment measured for blood pressure, TNF-α and IL-6 expression. The data represented as mean ± SD and analyzed by ANOVA (p less than 0.05) in any difference, a subsequent Duncan test. Data analyzed by SPSS 20.0 for Windows 10.Result: The results showed the treatment of VisBC could reduce systole (19.64%) and diastole (29.15%) blood pressure. Also, VisBC inhibited the expression of TNF-α (8.08%) and IL-6 (8.39%). The VisBC of tilapia with a dose of 150 mg/kg could inhibit the expression of TNF-α and IL-6, thereby affected the decrease in blood pressure (systole and diastole) in rats induced by DOCA-salt. This research proved that fish waste could produce bioactive as anti-hypertension.

scholarly journals Different blood pressure responses in hypertensive rats following chemerin mRNA inhibition in dietary high fat compared to dietary high-salt conditions

2019 ◽  
Vol 51 (11) ◽  
pp. 553-561 ◽  
Author(s):  
David J. Ferland ◽  
Emma D. Flood ◽  
Hannah Garver ◽  
Steve T. Yeh ◽  
Stanley Riney ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Antisense Oligonucleotides ◽  
Reduce Blood Pressure ◽  
High Salt ◽  
Whole Body ◽  
Sprague Dawley ◽  
High Fat ◽  
Hypertensive Rats ◽  
Blood Pressure Responses ◽  
Antihypertensive Treatments

Chemerin is a contractile adipokine, produced in liver and fat, and removal of the protein by antisense oligonucleotides (ASO) lowers blood pressure in the normal Sprague Dawley rat. In humans, chemerin is positively associated with blood pressure and obesity so we hypothesized that in a model of hypertension derived from high-fat (HF) feeding, the chemerin ASO would reduce blood pressure more than a high-salt (HS) model. Male Dahl S rats were given a HF (60% kcal fat; age 3–24 wk) or HS diet (4% salt; age 20–24 wk to match age and blood pressure of HF animals). Scrambled control, whole body, or liver-specific ASOs that knock down chemerin were delivered subcutaneously once per week for 4 wk with tissue and blood collected 2 days after the last injection. Conscious blood pressure was measured 24 h/day by radiotelemetry. By the end of whole body ASO administration, blood pressure of HF animals had fallen 29 ± 2 mmHg below baseline, while blood pressure of HS-diet animals fell by only 12 ± 4 mmHg below baseline. Administration of a liver-specific ASO to HF Dahl S resulted in a 6 ± 2 mmHg fall in blood pressure below baseline. Successful knockdown of chemerin in both the whole body and liver-specific administration was confirmed by Western and PCR. These results suggest that chemerin, not derived from liver but potentially from adipose tissue, is an important driver of hypertension associated with high fat. This knowledge could lead to the development of antihypertensive treatments specifically targeted to obesity-associated hypertension.

Na+, K+ and water balance in young spontaneously hypertensive rats: relationship to blood pressure after high K+ treatment

1987 ◽  
Vol 72 (3) ◽  
pp. 321-327 ◽  
Author(s):  
A. Louise Sugden ◽  
Barbara L. Bean ◽  
James A. Straw
Keyword(s):  
Blood Pressure ◽  
Water Balance ◽  
Spontaneously Hypertensive Rats ◽  
Urine Volume ◽  
Extracellular Fluid ◽  
Maximum Level ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
High K ◽  
Sufficient Magnitude

1. These studies were designed to investigate the effects of high dietary K+ on electrolyte and water balance in young spontaneously hypertensive rats (SHR) and to relate these effects to changes in blood pressure. 2. The high K+ diet reduced blood pressure by approximately 10 mmHg during the development of hypertension. Blood pressure, however, plateaued at the same maximum level as control by age 13 weeks. 3. Rats fed the high K+ diet showed a significant increase in water intake and urine volume throughout the treatment period but no change in plasma volume or extracellular fluid volume occurred. 4. A slight natriuresis was also observed in rats on the high K+ diet, but this was not of sufficient magnitude to decrease total body Na+. 5. These results confirm previous findings that K+ causes a diuresis and a natriuresis, but demonstrate that the diuretic action of K+ cannot explain its antihypertensive properties in young SHR.

Abstract 114: K+ Rich Diet During Angiotensin II Hypertension Reduces Renal Na-Cl Cotransporter and Phosphorylation, but Not Blood Pressure

Hypertension ◽  
2015 ◽  
Vol 66 (suppl_1) ◽  
Author(s):  
Luciana C Veiras ◽  
Jiyang Han ◽  
Donna L Ralph ◽  
Alicia A McDonough
Keyword(s):  
Blood Pressure ◽  
Urine Volume ◽  
Weight Ratio ◽  
Distal Tubule ◽  
Reduce Blood Pressure ◽  
Ang Ii ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
K Secretion ◽  
Pressure Natriuresis

During Ang II hypertension distal tubule Na-Cl Cotransporter (NCC) abundance and its activating phosphorylation (NCCp), as well as Epithelial Na+ channels (ENaC) abundance and activating cleavage are increased 1.5-3 fold. Fasting plasma [K+] is significantly lower in Ang II hypertension (3.3 ± 0.1 mM) versus controls (4.0 ± 0.1 mM), likely secondary to ENaC stimulation driving K+ secretion. The aim of this study was to test the hypothesis that doubling dietary K+ intake during Ang II infusion will lower NCC and NCCp abundance to increase Na+ delivery to ENaC to drive K+ excretion and reduce blood pressure. Methods: Male Sprague Dawley rats (225-250 g; n= 7-9/group) were treated over 2 weeks: 1) Control 1% K diet fed (C1K); 2) Ang II infused (400 ng/kg/min) 1% K diet fed (A1K); or 3) Ang II infused 2% K diet fed (A2K). Blood pressure (BP) was determined by tail cuff, electrolytes by flame photometry and transporters’ abundance by immunoblot of cortical homogenates. Results: As previously reported, Ang II infusion increased systolic BP (from 132 ± 5 to 197 ± 4 mmHg), urine volume (UV, 2.4 fold), urine Na+ (UNaV, 1.3 fold), heart /body weight ratio (1.23 fold) and clearance of endogenous Li+ (CLi, measures fluid volume leaving the proximal tubule, from 0.26 ± 0.02 to 0.51 ± 0.01 ml/min/kg) all evidence for pressure natriuresis. A2K rats exhibited normal plasma [K+] (4.6 ± 0.1 mM, unfasted), doubled urine K+ (UKV, from 0.20 to 0.44 mmol/hr), and increased CLi (to 0.8 ± 0.1 ml/min/kg) but UV, UNaV, cardiac hypertrophy and BP were unchanged versus the A1K group. As expected, NCC, NCCpS71 and NCCpT53 abundance increased in the A1K group to 1.5 ± 0.1, 2.9 ± 0.5 and 2.8 ± 0.4 fold versus C1K, respectively. As predicted by our hypothesis, when dietary K+ was doubled (A2K), Ang II infusion did not activate NCC, NCCpS71 nor NCCpT53 (0.91 ± 0.04, 1.3 ± 0.1 and 1.6 ± 0.2 fold versus C1K, respectively). ENaC subunit abundance and cleavage increased 1.5 to 3 fold in both A1K and A2K groups; ROMK was unaffected by Ang II or dietary K. In conclusion, evidence is presented that stimulation of NCC during Ang II hypertension is secondary to K+ deficiency driven by ENaC stimulation since doubling dietary K+ prevents the activation. The results also indicate that elevation in BP is independent of NCC activation

scholarly journals The effects of supraphysiological supplementation of b-carotene in spontaneously hypertensive rats (SHR and SHR-sp)

2014 ◽  
Vol 41 (5) ◽  
pp. 351-355 ◽  
Author(s):  
Stênio Karlos Alvim Fiorelli ◽  
Lúcia Marques Vianna ◽  
Carlos Alberto Basílio de Oliveira ◽  
Rossano Kepler Alvim Fiorelli ◽  
Bernardo Cunha Senra Barros ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Carotid Arteries ◽  
Morphological Changes ◽  
Serum Levels ◽  
Muscle Layer ◽  
Male Rats ◽  
Elastic Fibers ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Objective: to investigate the effect of administration of supraphysiological âcaroteno on biological parameters (ectoscopy and blood pressure), laboratory (malondialdehyde) and histological (liver and carotid arteries) of spontaneously hypertensive rats prone to stroke (SHR-sp).Methods: we used 36 male rats were divided into three groups, each containing 12 rats Wistar, SHR and SHR-sp, subdivided into six control animals and six animals treated with supraphysiological doses of âcaroteno for two periods of ten weeks interspersed with one week interruption. In the experiment were assessed daily physical examination and blood pressure (plethysmography). At sacrifice, blood was collected for measurement of serum malondialdehyde, liver and carotid arteries for histological examination.Results: temporary change in color of the fur, decreased significantly (p<0.0001) blood pressure (20mg supplementation âcaroteno) and serum levels of malondialdehyde (p<0.05) and increased amount of elastic fibers in the carotid wall of SHR and SHR-sp.Conclusion: supplementation of supraphysiological âcaroteno caused no toxic effects, showed positive response in the modulation of blood pressure and lower serum malondialdehyde. No significant morphological changes were found in both groups, except an increase in the number of elastic fibers in the muscle layer carotid suggesting elastosis in SHR and SHR-sp.

Abstract 9: Endothelin B Receptors Protect Against Hypertension Induced by Chronic Angiotensin II in Female Rats

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Wararat Kittikulsuth ◽  
David M Pollock
Keyword(s):  
Blood Pressure ◽  
High Salt ◽  
Female Rats ◽  
Male Rats ◽  
Ang Ii ◽  
Hypertensive Rats ◽  
High Salt Diet ◽  
Male And Female ◽  
Male And Female Rats ◽  
Endothelin B

Endothelin B (ET B ) receptors mediate vasodilation, anti-inflammation and natriuresis, which ultimately contribute to blood pressure control. We previously showed that renal medullary ET B receptor function is maintained in female angiotensin (Ang) II hypertensive rats, while male Ang II hypertensive rats have blunted ET B -induced natriuretic responses. Because female rats are more resistance to blood pressure elevation induced by high salt intake and/or Ang II infusion, we hypothesized that ET B receptors protect female rats against the hypertensive response and renal injury induced by a high salt diet and chronic Ang II infusion compared to males. Male and female rats received Ang II infusion (150 ng/kg/min; sc.) with 4% NaCl for 4 weeks; blood pressure was measured by telemetry. After a week of Ang II infusion with a high salt diet, subsets of both male and female rats received the ET B antagonist, A-192621, at three doses on consecutive weeks (1, 3, and 10 mg/kg/d in food). Male rats had a significantly higher blood pressure compared to females after 4 weeks of Ang II (178±10 vs. 138±10 mmHg; p<0.05). A-192621 resulted in a dose-dependent increase in blood pressure in female Ang II hypertensive rats (167±8 mmHg at 10 mg/kg/d; p<0.05); the increase produced by A-192621 in male Ang II hypertensive rats was not statistically significant (193±10 mmHg). After 4 weeks of Ang II infusion, the level of proteinuria and nephrinuria was higher in male rats compared to female. A-192621 did not further increase urinary excretion of protein or nephrin in both male and female Ang II hypertensive rats. In conclusion, these results support the hypothesis that ET B receptors provide more protection against hypertension during chronic Ang II infusion in female rats compared to male.

5-HT2B-receptor antagonist LY-272015 is antihypertensive in DOCA-salt-hypertensive rats

1999 ◽  
Vol 276 (3) ◽  
pp. H944-H952 ◽  
Author(s):  
Stephanie W. Watts ◽  
Gregory D. Fink
Keyword(s):  
Blood Pressure ◽  
Receptor Antagonist ◽  
High Blood Pressure ◽  
Reduce Blood Pressure ◽  
Receptor Expression ◽  
Hypertensive Rats ◽  
Arterial Blood ◽  
Salt Hypertension

We previously demonstrated a change in the receptors mediating 5-hydroxytryptamine (5-HT)-induced contraction in arteries of deoxycorticosterone acetate (DOCA)-salt-hypertensive rats. Specifically, contraction to 5-HT is mediated primarily by 5-HT2A receptors in arteries from normotensive sham rats and by both 5-HT2A and 5-HT2B receptors in arteries from hypertensive rats. We hypothesized that the 5-HT2B receptor may play a role in maintaining the high blood pressure of DOCA-salt-hypertensive rats, and herein we provide data connecting in vitro and in vivo findings. The endothelium-denuded isolated superior mesenteric artery of DOCA-salt rats displayed a marked increase in maximum contraction to the newly available 5-HT2B-receptor agonist BW-723C86 compared with that of arteries from sham rats, confirming that the 5-HT2B receptor plays a greater role in 5-HT-induced contraction in arteries from DOCA-salt rats. In chronically instrumented rats, the 5-HT2B-receptor antagonist LY-272015 (0.3, 1.0, and 3.0 mg/kg iv at 30-min intervals) was given cumulatively 1 time/wk during 4 wk of continued DOCA-salt treatment. LY-272015 did not reduce blood pressure of the sham-treated rats at any time or dose. However, LY-272015 (1.0 and 3.0 mg/kg) significantly reduced mean blood pressure in a subgroup of week 3 (−20 mmHg) and week 4 DOCA-salt (−40 mmHg) rats that had extremely high blood pressure (mean arterial blood pressure ∼200 mmHg). Blockade of 5-HT2Breceptors by in vivo administration of LY-272015 (3.0 mg/kg) was verified by observing reduced 5-HT-induced contraction in rat stomach fundus, the tissue from which the 5-HT2B receptor was originally cloned. These data support the novel hypothesis that 5-HT2B-receptor expression is induced during the development of DOCA-salt hypertension and contributes to the maintenance of severe blood pressure elevations.

Blood-pressure lowering efficacy of winged bean seed hydrolysate in spontaneously hypertensive rats, peptide characterization and a toxicity study in Sprague-Dawley rats

2018 ◽  
Vol 9 (3) ◽  
pp. 1657-1671 ◽  
Author(s):  
Shyan Yea Chay ◽  
Annas Salleh ◽  
Nor Fazila Sulaiman ◽  
Najib Zainal Abidin ◽  
Mohamad Ariff Hanafi ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Good Manufacturing Practice ◽  
Reduce Blood Pressure ◽  
Winged Bean ◽  
Bean Seed ◽  
Sprague Dawley ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Pressure Lowering

Winged bean seed hydrolysate is found to reduce blood pressure in spontaneously hypertensive rats. Peptide of non-Current Good Manufacturing Practice grade demonstrates toxicity and is not suitable for testing in animals.

Sardine proteins (Sardina pilchardus) combined with green lemon zest (Citrus latifolia) improve blood pressure, lipid profile and redox status in diabetic hypertensive rats

2018 ◽  
Vol 48 (4) ◽  
pp. 654-668 ◽  
Author(s):  
HadjMostefa Khelladi ◽  
Djamil Krouf ◽  
Nawal Taleb-Dida
Keyword(s):  
Blood Pressure ◽  
Redox Status ◽  
Cardiovascular Complications ◽  
Reduce Blood Pressure ◽  
Diabetic Patients ◽  
Hypertensive Rats ◽  
Content Type ◽  
Protein Group ◽  
Male Wistar Rats ◽  
Tbars Values

Purpose This paper aims to study the effect of green lemon zest combined with sardine proteins in diabetic hypertensive rats (DHRs). Design/methodology/approach Male Wistar rats (n = 30) weighing 250 ± 10 g were divided into five groups. The first group consumed a diet containing 20 per cent casein (C). The other four groups are rendered diabetic by intraperitoneal injection of streptozotocin (40 mg/kg body weight), then hypertensive by subcutaneous implantation controlled time-release pellet containing ouabain (0.25 mg/pellet). One untreated group (DHR) consumed 20 per cent casein and the three other groups consumed the same diet supplemented with 2 per cent green lemon zest (DHR-lz), or with 20 per cent of sardine protein (group DHR-sp) or with the combination of both sardine proteins and green lemon zest (group DHR-sp + lz). Findings DHRs feeding on the combination of both sardine protein (sp) and lemon zest (lz) induced a significant decrease of diastolic blood pressure and heart rates values compared with DHR (p < 0.05). The HDLC values were increased by +55 per cent in DHR-sp + lz compared with DHR group. Moreover, plasma non-HDLC concentrations were decreased significantly compared to DHR, DHR-lz, DHR-sp and C groups. In DHR-sp + lzvs DHR group, TBARS values were decreased by −25 per cent in the liver. Moreover, kidney TBARS were significantly reduced by −66, −51, −65 and −67 per cent compared with C, DHR, DHR-lz and DHR-sp, respectively. Originality/value These results suggest that consumption of green lemon zest combined with sardine proteins can reduce blood pressure and tissue oxidative damage and, therefore, help to prevent cardiovascular complications in hypertensive diabetic patients.

scholarly journals Piper sarmentosum Leaves Aqueous Extract Attenuates Vascular Endothelial Dysfunction in Spontaneously Hypertensive Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Fatimatuzzahra Hashim Fauzy ◽  
Maizura Mohd Zainudin ◽  
Hidayatul Radziah Ismawi ◽  
Taher F. T. Elshami
Keyword(s):  
Blood Pressure ◽  
Aqueous Extract ◽  
Spontaneously Hypertensive Rats ◽  
Positive Control ◽  
No Production ◽  
Hypertensive Rats ◽  
Traditional Use ◽  
Spontaneously Hypertensive ◽  
Treatment Groups ◽  
Endothelin System

Piper sarmentosum is a tropical plant in Southeast Asia known for its traditional use in curing various ailments including hypertension. Previous research works have provided evidence for the herb’s antihypertensive property. However, the exact mechanisms involved are still in question. The present study investigated the effects of Piper sarmentosum leaves aqueous extract (PSAE) treatment on vascular endothelin system in spontaneously hypertensive rats (SHRs). Four groups of SHRs were treated for 28 consecutive days, with negative and positive control groups receiving distilled water and 3 mg/kg perindopril, respectively. Another two groups are the treatment groups, which received PSAE and combination of 1.5 mg/kg perindopril and PSAE. Weekly measurements of blood pressure showed that PSAE significantly reduced the systolic, diastolic, and mean arterial pressures (P<0.05) of the rats. PSAE also increased mesenteric artery nitric oxide (NO) level (P<0.05) and reduced endothelin-1 (ET-1) level (P<0.05) in the treatment groups. Our results demonstrate that oral administration of PSAE reduced blood pressure in SHRs by reducing the ET-1 level while increasing NO production.

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