scholarly journals PRIMACY OF LIQUID MEDIUM TECHNIQUE ON PROTOCORM LIKE BODIES PROPAGATION OF Phalaenopsis sp ORCHIDS IN TISSUE CULTURE

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Budi Kriswanto ◽  
Sigit Soeparjono ◽  
Didik Pudji Restanto
Keyword(s):  
Tissue Culture ◽  
Liquid Medium ◽  
Concentration Ratio ◽  
Solid Medium ◽  
Basal Medium ◽  
Single Factor ◽  
Duncan Multiple Range Test ◽  
Plant Propagation ◽  
Medium Type ◽  
Range Test

Tissue culture have been used for plant propagation generally, and the medium has been important role in its growth. Vegetative propagation on Phalaenopsis sp orchids can be through the protocorm like bodies (PLB). Medium of affect on propagation of PLB was carried out on medium type, kind of basal medium and concentrations ratio of naphtaleneacetic acid (NAA) and benzylamino purine (BAP). The experiment used Completely Randomized Factorial Design with 3 replications and continued with the Duncan Multiple Range Test (DMRT) if there were significant differences. The results showed that the best callus formed in a combination of solid medium type and Murashige & Skoog (MS) basal medium was 100%. The most number of PLB produced from a combination of liquid medium types and a concentration ratio of NAA 1 mgL-1 and BAP 5 mgL-1, the most number of plantlet produced from a combination of MS basal medium and the concentrations ratio of NAA 0.1 mgL-1 and BAP 0.1 mgL-1, the number of PLB germination and PLB with leaves were influenced by each single factor.

scholarly journals PENGARUH PERBEDAAN DOSIS LARUTAN PUPUK NPK MAJEMUK TERHADAP KEBERHASILAN PERTUMBUHAN BAWANG MERAH (Allium ascalonicum L.)

2021 ◽  
Vol 46 (2) ◽  
pp. 219
Author(s):  
Khusna Amalia Damayanti ◽  
Prasodjo Soedomo
Keyword(s):  
Experimental Design ◽  
Research Method ◽  
Block Design ◽  
Maximum Growth ◽  
Growth And Yield ◽  
Single Factor ◽  
Duncan Multiple Range Test ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Range Test

 This research aims to determine the type of shallot variety and the correct dosage for maximum growth and yield of shallot plants. The research method used an experimental method with the experimental design used was a single factor randomized block design (RBD) consisting of 6 treatments, namely A (Sembrani Variety and 100). ml/polybag), B (Sembrani variety and 120 ml/polybag), C (Sembrani variety and 140 ml/polybag), D (Mente's variety and 100 ml/polybag), E (Mente's variety and 120 ml/polybag), and F (Mente's variety and 140 ml/polybag) was repeated 4 times so that there were a total of 24 experimental units. The effect of the treatment was analyzed using variance and if the F test at the 5% level was significant, then to find out the best treatment was continued with the DMRT (Duncan Multiple Range Test) further test at the 5% level. The results showed that there was a significant effect on the types of shallot varieties with different dosages of NPK compound, on all research parameters. Treatment E gave the highest yield at plant height (44.49 cm), the weight of fresh tubers with leaves/clump (7.11 g), the weight of fresh tubers with leaves/plot (19.93 g), the weight of dry tubers with leaves/clump (5.90 g), weight dry tubers with leaves/plot (16.35 g). While treatment D gave the highest yield on the number of tillers (3.14), the number of leaves (6.97), the number of tubers (3.14). Treatment C gave the highest yield on tuber diameter (22.58 mm) and treatment A tuber length (31.48 mm).   

scholarly journals MULTIPLIKASI TANAMAN NILAM (Pogostemon cablin Benth) var. TAPAK TUAN SECARA IN VITRO

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
SITI ASIYAH ◽  
CHRISTIANI TUMILISAR ◽  
TUTI LESTARI
Keyword(s):  
Tissue Culture ◽  
Leaf Explants ◽  
Stem Explants ◽  
Plant Propagation ◽  
In Vitro Techniques ◽  
Pogostemon Cablin ◽  
Shoot Number ◽  
Independent Variable ◽  
Range Test

ABSTRACTTissue culture is a technique widely used for propagation and improvement of crop quality. One way of plant propagation can be done with in vitro techniques. The purpose of the study was to determine the combinations and concentrations of NAA and BAP are appropriate for plant propagation patchouli. Dependent variable is the time of initiation of callus and shoots, callus texture, color callus, shoot number, and the number of roots patchouli. Independent variable is the concentration of growth regulators NAA and BAP. The method in this study is the experimental method. Data were analyzed by two-way Anova test followed by Duncan’s Multiple Range Test (DMRT). The results showed the stem explants concentrations of NAA and BAP best to induce callus is without NAA and BAP (control), 0.1 ppm NAA without BAP, NAA + BAP 0.5 ppm 0, while the leaf explants is there a 0.1 ppm NAA without BAP, NAA 0.1 ppm BAP + 0.5 ppm, 0.5 ppm BAP without NAA and NAA + BAP 0:01 ppm 1 ppm. Highest number of shoots obtained at a concentration of 0 ppm NAA concentration and NAA and 0.01 for leaf explants at a concentration of 0:01 ppm NAA + BAP 1 ppm and 0 ppm NAA + BAP 0.5 ppm for stem explants. Highest number of roots was found in 0:01 ppm for NAA concentration and leaf explants at a concentration of 0.1 ppm NAA + BAP and NAA 00:01 0 ppm ppm + 0.5 ppm BAP stem explants. Results of statistical analysis showed that there were significant differences of NAA and BAP concentrations on plant propagation patchouli (Pogostemon cablin Benth). Keywords: tissue culture, NAA, BAP, plant nilam (Pogostemon cablin Benth)

scholarly journals The Relationships Among Physiological Characters and Productivities of Nine PGL Clones in Medium Land

2016 ◽  
Vol 18 (3) ◽  
pp. 160
Author(s):  
Ika Irmayanti ◽  
Didik Indradewa ◽  
Eka Tarwaca Susila Putra
Keyword(s):  
Correlation Analysis ◽  
Significant Positive Correlation ◽  
Stomatal Density ◽  
Block Design ◽  
Single Factor ◽  
Duncan Multiple Range Test ◽  
Several Variables ◽  
Randomized Complete Block Design ◽  
Range Test ◽  
Physiological Characters

The objectives of the study were to determine the relationships among the physiological characters and productivities of nine PGL clones in medium land. Field trial was arranged in a Randomized Complete Block Design (RCBD) single factor with three blocks as replications. The treatment was PGL clones, consisting of nine clones, namely PGL 1 PGL 3, 4 PGL, PGL 7, PGL 10, PGL 11, PGL 12, PGL 15, and PGL 17. The observations were done on several variables of physiological characters and productivities. Data were analyzed by using the Analysis of Variance (ANOVA) at 5% levels. If there were significant differences among the treatments, they will be analysed by Duncan Multiple Range Test (DMRT). The relationships among variables were determined using correlation analysis. Results showed that there were wide variations in the stomatal density and width openings  and also productivities among the nine PGL clones. Stomatal density and width openings have significant positive correlation with the productivity. PGL 12 and 15 with a denser of stomatal arrangement and wider stomatal openings have higher productivities when compared to other PGL clones, especially PGL 7 with the most loosely stomatal arrangement and narrower stomatal openings.

scholarly journals Pengujian Efektivitas Tipe Pemangkasan Terhadap Produksi Tiga Varietas Semangka Pada Hidroponik Sistem Fertigasi (Drip Irrigation)

2018 ◽  
Vol 6 (2) ◽  
pp. 103
Author(s):  
Rommy Andhika Laksono
Keyword(s):  
Research Method ◽  
Fruit Weight ◽  
Agricultural Technology ◽  
Real Difference ◽  
Single Factor ◽  
Duncan Multiple Range Test ◽  
Fresh State ◽  
Screen House ◽  
Range Test ◽  
Fruit Diameter

Watermelon is an annual horticultural commodity that is easily damaged (perishable) so it must be consumed in a fresh state and must be marketed after harvest. Opportunities to increase production and productivity of watermelon in Indonesia are still very wide open both through the intensification and improvement of agricultural technology. The purpose of this study is to study and obtain the type of pruning that provides the highest production in hydroponics watermelon cultivation Fertigation system. The experiment was conducted at Screen House Mekarsari Fruit Garden Bogor Regency. from April to Augustus 2018. The research method used is experimental method and experimental design used is Single Factor Random Design, which consists of 9 treatments in 3 repetitions. The usual is a combination of two types of cutting (Toping and Pruning) and three watermelon varieties (Amara F1, Angela F1, and Batu Sengkaling). The data of the research using the analysis of variance based on F test of 5% level. If there is a real difference, the analysis is continued with DMRT (Duncan Multiple Range Test) test of 5% level.The results showed that the application of the cutting type had a significant effect on the stem diameter of 21 HST, 28 HST, and 35 HST, flowering age, harvest age, fruit diameter, and fruit weight per plant, three watermelon varieties in the hydroponic fertigation system. Application of Toping Trimming on Amara F1 Varieties provides the highest watermelon yields of 39, 24 tonnes per hectare or 8.24 kg per plant in the hydroponic fertigasi system.

UJI DAYA HASIL BEBERAPA CALON VARIETAS JAGUNG HIBRIDA DI PT. MULYA AGRO SARANA

2019 ◽  
Vol 3 (2) ◽  
pp. 120-128
Author(s):  
Evan Yonda Pratama ◽  
Riski Hasputri ◽  
Rudi Tejo Setiyono
Keyword(s):  
Duncan Multiple Range Test ◽  
Range Test

Jagung merupakan salah satu sumber komoditas tanaman pangan yang memiliki peranan penting dan strategis dalam pembangunan nasional. Beberapa faktor yang perlu diperhatikan dalam upaya peningkatan produksi jagung adalah penggunaan varietas unggul baru, pemupukan dan pengaturan populasi tanam. Salah satu komponen teknologi yang paling mudah diadopsi oleh petani adalah Varietas Unggul Baru (VUB) yang memiliki daya hasil yang tinggi. Tujuan penelitian ini adalah untuk menentukan calon varietas jagung hibrida yang memiliki hasil yang lebih baik. Penelitian ini dilaksanakan di Kebun Percobaan PT Mulya Agro Sarana, Desa Wonokerto, Kecamatan Plemahan, Kabupaten Kediri, Jawa Timur pada April sampai Agustus 2018. Penelitian menggunakan Rancangan Acak Kelompok yang terdiri dari 5 perlakuan dan 3 ulangan. Perlakuan meliputi 4 calon varietas jagung hibrida MASB1, MASB2, MASB3, MASB4, dan satu varietas jagung hibrida sebagai standar yaitu varietas Bima 20 Uri. Data yang diperoleh dianalisis menggunakan sidik ragam, jika berbeda nyata akan dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) α = 5%. Hasil penelitian menunjukkan bahwa calon varietas jagung hibrida yang prospektif dikembangkan lebih lanjut yaitu MASB3 dan MASB4, hal ini terlihat pada bobot 1000 butir dan produktivitas ton/ha. Data produktivitas adalah MASB3 sebesar 12.16 ton/ha dan MASB4 sebesar 14.18 ton/ha.

PENGARUH LAMA PENGERINGAN DENGAN SUHU YANG BERBEDA TERHADAP PERUBAHAN WARNA DAN RASA CABAI MERAH BESAR (Capsicum annum L.)

2019 ◽  
Vol 3 (1) ◽  
pp. 53-66
Author(s):  
Sukarman Hadi Jaya Putra ◽  
Maria Stefina Asriyani
Keyword(s):  
Duncan Multiple Range Test ◽  
Capsicum Annum ◽  
Range Test

Cabai merah besar memiliki nilai ekonomi tinggi, namun cabai merah besar termasuk dalam jenis buah yang mudah rusak. Perlakuanpascapanen yang tepat dibutuhkan, salah satunya melalui proses pengeringan yang sering digunakan secara mekanis dengan waktu pengeringan dan suhu yang berbeda. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh berapa lama pengeringan dengan suhu yang berbeda melalui perubahan karakteristik cabai merah besar (Capsicum annum L.). Penelitian menggunakan rancangan acak lengkap (RAL) dengan tiga kali pengulangan. Temperatur pengeringan yang digunakan adalah S1 (50 °C), S2 (55 °C), S3 (60 °C) dan S4 (65 °C). Waktu pengeringan yang digunakan L1 (20 jam), L2 (23 jam), dan L3 (26 jam). Pengamatan parametrik terdiri dari warna, tekstur, dan rasa. Analisis data yang digunakan adalah analisis varians (ANOVA) 95% (α=0,95) dan uji lanjut dengan Duncan Multiple Range Test (DMRT)5%. Observasi digunakan dengan kuesioner. Hasil penelitian menunjukkan bahwa proses pengeringan dengan suhu yang berbeda berpengaruh terhadap perubahan warna dan rasa cabai merah besar.

RESPON TANAMAN KEDELAI VARIETAS CENENG PADA INTENSITAS CAHAYA BERBEDA

2018 ◽  
Vol 2 (2) ◽  
pp. 98-112
Author(s):  
Laili Munawaroh ◽  
Ummu Kalsum ◽  
Purwanti Budi Laksono ◽  
Irwan Siallagan
Keyword(s):  
Duncan Multiple Range Test ◽  
Range Test

Tanaman yang ternaungi mengakibatkan ketersediaan cahaya menjadi berkurang terutama pada intensitas cahaya. Perbedaan karakteristik tanaman yang diatur oleh gennya menyebabkan kemampuan beradaptasi terhadap kondisi ternaungi menjadi berbeda. Tujuan dari penelitian ini adalah mengamati respon tanaman kedelai varietas Ceneng pada kondisi lingkungan dengan intensitas cahaya yang berbeda. Perlakuan pada penelitian ini menggunakan 1 faktor, yaitu naungan. Perlakuan tersebut meliputi perlakuan naungan ± 59% menggunakan pohon pada 0 minggu setelah tanam (MST), naungan paranet pada 8 MST dan tanpa naungan sebagai kontrol. Variabel yang diamati adalah tinggi tanaman, jumlah daun, jumlah cabang, lebar dan panjang daun, waktu berbunga, jumlah bunga, jumlah polong total, jumlah polong hampa dan polong isi, kandungan klorofil serta gula pada daun. Data yang diperoleh dianalisis menggunakan analysis of varians (anova) dengan taraf α = 5%. Hasil uji anova yang signifikan berbeda dilanjutkan dengan uji Duncan Multiple Range Test (DMRT) dengan taraf α = 5%. Hasil penelitian menunjukkan bahwa perlakuan naungan pada tanaman kedelai varietas Ceneng meningkatkan kandungan klorofil a, klorofil b dan karotenoid daun, namun kadar antosianin menjadi menurun. Perlakuan naungan 59% dan 8 MST memberikan rata-rata kandungan gula yang lebih rendah dibandingkan tanpa naungan. Perlakuan naungan pada kedelai varietas Ceneng yang cocok adalah naungan 8 MST.

scholarly journals PENGARUH INOKULAN BAKTERI ASAM LAKTAT DAN ADITIF TERHADAP KUALITAS DAN KARAKTERISTIK SILASE SORGUM MUTAN 2 1 BROWN MIDRIB (Sorghum bicolor L. Moench)

Pastura ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 40
Author(s):  
R. Sriagtula ◽  
I. Martaguri ◽  
J. Hellyward ◽  
S. Sowmen
Keyword(s):  
Sorghum Bicolor ◽  
Brown Midrib ◽  
Duncan Multiple Range Test ◽  
Sorghum Bicolor L ◽  
Range Test

Penelitian ini bertujuan untuk mengobservasi pengaruh penambahan inokulasi bakteri asam laktat (BAL) dan aditif terhadap kualitas dan karakterietik silase whole crop sorgum mutan brown midrib (Sorghum bicolor L. Moench) galur Patir 3.7 yang dipanen pada fase soft dough. Penelitian dilaksanakan secara eksperimen menggunakan rancangan acak lengkap pola faktorial dengan 4 ulangan. Faktor A yaitu A1 = tanpa BAL, A2= penambahan BAL. Faktor B terdiri dari B1= tanpa aditif, B2= dedak, B3= jagung. Sumber BAL yang digunakan berasal dari inokulan komersil dari minuman fermentasi merk Yakult dengan dosis 1 ml (v/w) atau 11×109 CFU/ml/berat segar. Aditif terdiri dari dedak padi dan jagung halus digunakan sebanyak 3% (g/g)/berat segar. Parameter yang diamati adalah karakteristik dan kualitas silase meliputi nilai pH, nilai fleigh (NF), kandungan bahan kering (BK), protein kasar (PK), serat kasar (SK), lemak kasar (LK) dan Abu. Data dianalisis berdasarkan analisis keragaman menurut Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa tidak terdapat interaksi (P>0,05) antara penambahan BAL dan aditif terhadap pH, NF, BK, PK, SK, LK dan abu, sedangkan faktor tunggal adititif memberikan pengaruh berbeda nyata (P<0,05) lebih tinggi terhadap kandungan BK silase whole crop sorgum mutan BMR. Dari penelitian ini dapat disimpulkan bahwa secara umum penambahan inokulan BAL dan aditif menghasilkan karakteristik dan kualitas silase yang sama, namun demikian penambahan dedak padi dan jagung halus menghasilkan BK silase yang lebih tinggi dibanding tanpa BAL dan aditif. Kata kunci: aditif, BAL, brown midrib, silase, sorgum

scholarly journals A Novel 6-Benzyl Ether Benzoxaborole Is Active against Mycobacterium tuberculosisIn Vitro

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Nipul Patel ◽  
Theresa O'Malley ◽  
Yong-Kang Zhang ◽  
Yi Xia ◽  
Bjorn Sunde ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Liquid Medium ◽  
Inhibitory Concentration ◽  
Solid Medium ◽  
Eukaryotic Cells ◽  
Intracellular Bacteria ◽  
Benzyl Ether ◽  
Content Type ◽  
Resistant Mutants

ABSTRACT We identified a novel 6-benzyl ether benzoxaborole with potent activity against Mycobacterium tuberculosis. The compound had an MIC of 2 μM in liquid medium. The compound was also able to prevent growth on solid medium at 0.8 μM and was active against intracellular bacteria (50% inhibitory concentration [IC50] = 3.6 μM) without cytotoxicity against eukaryotic cells (IC50 > 100 μM). We isolated resistant mutants (MIC ≥ 100 μM), which had mutations in Rv1683, Rv3068c, and Rv0047c.

A medium, solidified with gellan gum, for determining the Na+ requirement of Vibrio species

1993 ◽  
Vol 39 (8) ◽  
pp. 804-808 ◽  
Author(s):  
Lisa D. Noble ◽  
John A. Gow
Keyword(s):  
Marine Bacteria ◽  
Solid Medium ◽  
Specific Growth ◽  
Basal Medium ◽  
Gellan Gum ◽  
Growth Responses ◽  
Prolonged Incubation ◽  
Low Sodium ◽  
Growth Requirements ◽  
Lag Period

Until now there has not been a satisfactory solid medium for determining the growth responses, to Na+, of marine and other bacteria that have specific growth requirements for Na+. A solid medium would be useful to investigators who would like to take advantage of the efficiency of multipoint inoculation when testing for a Na+ requirement. By using 1% gellan gum (Gel-GroTM) as the solidifying agent a medium was formulated that had a contaminating level of Na+ of slightly less than 2 mM in the basal medium. Two species of Aeromonas, which do not require Na+ for growth, and 31 species of Vibrio, which require Na+, were tested for their growth responses to Na+ on this medium. The Aeromonas strains grew well, within 24 h, at all of the Na+ concentrations tested. Approximately 75% of the Vibrio strains did not grow on the basal medium even after a prolonged incubation period. The remaining species were able to grow on the basal medium, but not without a lag period. These lag periods were as short as 36 h for two of the species and in some instances as long as 312 h. These lag periods were of sufficient duration to determine that Na+ stimulated the growth of the Vibrio strains that were able to grow on the basal medium. Approximately 75% of the strains, representing most species of Vibrio, were able to grow if as little as 25 mM Na+ was present in the medium.Key words: low-sodium medium, Na+ requirement, gellan gum, agar substitute, marine bacteria.

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