scholarly journals UJI CEPAT (RAPID TEST) ANTIBODI IgM TERHADAP Salmonella typhi DEMAM TIFOID

2018 ◽  
Vol 14 (3) ◽  
pp. 109
Author(s):  
Rini Riyant ◽  
Prihatini . ◽  
Siti Rochmatoen
Keyword(s):  
Typhoid Fever ◽  
Dengue Hemorrhagic Fever ◽  
Predictive Value ◽  
Rapid Test ◽  
Hemorrhagic Fever ◽  
Diagnostic Value ◽  
Salmonella Typhi ◽  
Diagnostic Efficiency ◽  
Igm Antibodies ◽  
Health Centres

Typhoid fever is caused by Salmonella typhi. The definitive diagnosis can be made by isolation of Salmonella typhi from blood, bone marrow or other body fluids. To support the clinical diagnosis of typhoid fever in Indonesia, where most hospitals and health centres haveno facilities for cultures, a rapid test for the detection of lipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies was evaluatedon serum samples from patients with typhoid fever. This study is proposed to know the rapid test diagnostic value for the detection oflipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies. A cross sectional, observational analytical study on 27 typhoidfever and 25 dengue hemorrhagic fever patients of the Dr. Soetomo Hospital, Dr. M Soewandhi General Hospital and Gotong-RoyongClinic has been conducted from January – May 2007. The diagnosis of typhoid fever patients was based on positive gall culture whilethe diagnosis of dengue hemorrhagic fever was based on negative gall culture, positive serology examination for dengue hemorrhagicfever and a recovery from dengue hemorrhagic fever with standard treatment. The sera from patients were examined using a rapid testfor the detection of lipopolysacharides (LPS) Salmonella typhi specific IgM antibodies from Amgenix Onsight of the first blood samples(collected on admission to the hospital) the rapid test for IgM antibodies showed the following: sensitivity 70.4%, specificity 80.0%,positive predictive value 79.2%, negative predictive value 71.4%, diagnostic efficiency 75% respectively. Of the second blood samples(collected 2–3 weeks during the illness) therapid test for IgM antibodies showed the following: sensitivity 88.9%, positive predictive value 82.8%, negative predictive value 87.0%, and diagnostic efficiency 84.6% respectively. The rapid test for IgM antibodies has a high diagnostic value for typhoid fever. The assay uses stabilized components which can be stored at room temperature; the test does notrequire special equipment and may be used in health centres that have no facilities for culture.

DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR

2016 ◽  
Vol 21 (3) ◽  
pp. 261
Author(s):  
Ranti Permatasari ◽  
Aryati Aryati ◽  
Budi Arifah
Keyword(s):  
Hepatitis C ◽  
Predictive Value ◽  
Core Protein ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Hcv Infection ◽  
Antibody Detection ◽  
Hcv Rna ◽  
Hcv Antibody

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to the recipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid test that could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multiple antibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. The samples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infection test using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatography method showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictive value of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody pattern was four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies. Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method has a good diagnostic value.

scholarly journals Diagnosa Penyakit Demam Berdarah Dengue (DBD) menggunakan Metode Learning Vector Quantization (LVQ)

2020 ◽  
Vol 4 (3) ◽  
pp. 56
Author(s):  
Firman Tawakal ◽  
Ahmedika Azkiya
Keyword(s):  
Neural Network ◽  
Typhoid Fever ◽  
Vector Quantization ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Learning Vector Quantization ◽  
Mean Square ◽  
Northern Australia ◽  
Neural Network Learning ◽  
Network Learning

Dengue Hemorrhagic Fever is a disease that is carried and transmitted through the mosquito Aedes aegypti and Aedes albopictus which is commonly found in tropical and subtropical regions such as in Indonesia to Northern Australia. in 2013 there are 2.35 million reported cases, which is 37,687 case is heavy cases of DHF. DHF’s symthoms have a similarity with typhoid fever, it often occur wrong handling. Therefore we need a system that is able to diagnose the disease suffered by patients, so that they can recognize whether the patient has DHF or Typhoid. The system will be built using Neural Network Learning Vector Quantization (LVQ) based on the best training results. This research is to diagnose Dengue Hemorrhagic Fever using LVQ with input parameters are hemoglobin, leukocytes, platelets, and heritrocytes. Based on result, the best accuracy is 97,14% with Mean Square Error (MSE) is 0.028571 with 84 train data and 36 test data. Conclution from the research is LVQ method can diagnose DHF Keywords: Dengue Hemorrhagic Fever; Learning Vector Quantization; classification; Neural Network;

scholarly journals A Patient with Typhoid Hepatitis

2021 ◽  
Vol 22 (2) ◽  
pp. 154-158
Author(s):  
M Vitanata Arfijanto ◽  
Isty Rindryastuti
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Gastrointestinal Symptoms ◽  
Signs And Symptoms ◽  
Hemorrhagic Fever ◽  
Liver Function Tests ◽  
Salmonella Typhi ◽  
Abnormal Liver Function ◽  
Grade Ii ◽  
Ig G

Typhoid hepatitis is typhoid fever accompanied by symptoms of jaundice, hepatomegaly and abnormal liver function tests. The incidence varies between 0.4% -26% of typhoid fever patients. We report a case of a 34-year-old male, presented with fever, epistaxis, gastrointestinal symptoms, thrombocytopenia and elevated AST/ ALT, thus the patient was first diagnosed as dengue hemorrhagic fever grade II. On day 9th the signs and symptoms were persisted, Ig M and Ig G Dengue was negative. Then we evaluated the virus marker for hepatitis and blood culture. The results were negative for HBsAg, anti HCV and Ig M anti HAV, but Salmonella typhi detected on blood culture. The patient was treated with ceftriaxone 1000mg bid iv and get better then discharged from hospital.

scholarly journals Utility of A Single Widal Test in The Diagnosis of Typhoid Fever

2012 ◽  
Vol 35 (2) ◽  
pp. 53-58 ◽  
Author(s):  
ABM Shahidul Alam ◽  
Fahim Ahmed Rupam ◽  
Farhana Chaiti
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Predictive Value ◽  
Salmonella Typhi ◽  
Definitive Diagnosis ◽  
Blood Cultures ◽  
Widal Test ◽  
Female Ratio ◽  
The Mean ◽  
Agglutinin Titer

Background & objectives: The clinical diagnosis of typhoid fever is difficult, as the presenting symptoms are often diverse and similar to those observed with other febrile illnesses. The definitive diagnosis of typhoid fever requires the isolation of Salmonella typhi or paratyphi from the patient concerned. Since patients often receive antibiotics prior to a confirmatory diagnosis, there is uncertainty that bacteria can be isolated from the blood cultures. Besides this, the facilities for blood culture are not always available or feasible. All these limitations have made Widal test the most utilized diagnostic test for typhoid fever. Many studies have produced data which had cast serious doubts on the value of the Widal Test and thus reappraisal of the role of a single Widal test is needed.Methods & materials: This study was carried out to determine the changes in clinical pattern of enteric fever. A total of 153 children, aged 0 to 14 years, diagnosed as typhoid fever (either positive blood culture for Salmonella typhi or paratyphi) were induced in the study. Of them, 86 children were with a definitive diagnosis of typhoid or paratyphoid fever as indicated by the isolation of S. typhi or S. paratyphi from the blood and 17 had negative blood culture but were clinically suspected of having typhoid fever. The control group was comprised of 50 children with non-typhoidal fevers The Widal test was carried out using rapid slide agglutination method and its accuracy was assessed by comparing the findings with that obtained through blood culture.Result: The mean age of the patients was 5.2 ± 2.8 years and the youngest and oldest patients were 0.7 and 14 years respectively and male to female ratio was roughly 1:1. Nearly one-quarter (24.6%) of the patients had been suffering from the disease for >10 days and the mean duration of illness was 8.2 ± 3.3 days. Widal Test result showed that an ‘O’ agglutinin titer of cut-off value e”1:40 gave a sensitivity of 87.2%, a specificity of 47.1%, a positive predictive value (PPV) of 89.2% and a negative predictive value (NPV) of 42.1%. The sensitivity and NPV decreased with the increase in titer levels and were 56.9% and 31.5% at cut-off value of e” 1:320, while the specificity and PPV increased with the increase in titer levels from 47.1% and 89.2% respectively at a titer of e”1:40 to 100% at a titer of e” 1:320. The ‘titer behaved in the same way as did the ‘O’ agglutinin titer. Similarly when H’ agglutinin was used the sensitivity and NPV decreased from 65% and 31.7% at a titer of e”1:40 to only 25% and 20% respectively at a titer of > 1:320, while specificity and PPV increased from 76.4% and 81.1% at >1:40 to 94.1% and 95.6% respectively at e” 1:320. When either ‘O’ or ‘H’ antibody titer of e”1:160 was used, a good sensitivity (71%), specificity (70.6%) and PPV (92.4%) resulted, though NPV decreased to 32.4%.Conclusion: The Widal test can be of diagnostic value when blood cultures are not available nor practically feasible.DOI: http://dx.doi.org/10.3329/bjch.v35i2.10377  Bangladesh J Child Health 2011; Vol 35 (2): 53-58

scholarly journals Spatial distribution of Infectious Disease Epidemic in The Soppeng Regency, 2016-2018

2021 ◽  
Vol 940 (1) ◽  
pp. 012017
Author(s):  
Basri ◽  
Tasrifin Tahara ◽  
Dinna Dayana La Ode Malim ◽  
La Ode Abdul Munafi
Keyword(s):  
Infectious Disease ◽  
Spatial Distribution ◽  
Infectious Diseases ◽  
Typhoid Fever ◽  
Dengue Hemorrhagic Fever ◽  
Spatial Data ◽  
Health Centre ◽  
Hemorrhagic Fever ◽  
Development Planning ◽  
Public Health Centre

Abstract Diarrhea, typhoid fever and dengue hemorrhagic fever (DHF) are environmentalbased infectious diseases that contribute to the mortality rate of humans. This paper investigates the spatial distribution and the infectious disease epidemic that occurs based on environmental factors. The three primary diseases analyzed were diarrhea, typhoid fever, and dengue hemorrhagic fever. We abstracted data from several sources, including administrative maps, Regional Spatial Planning, BAPPEDA Soppeng Regency, the Central Statistics Agency (BPS), Public Health Centre, RBI Maps, and National DEM. The tool used in this research is a computer equipped with ArcGIS. The analysis documented that the trend of the three primary diseases did not represent a consistent decline in three consecutive years and even increased in certain subdistricts. Spatial data shows that the spread of infectious diseases based on the incidence rate is still dominated at low levels, although medium and high IR categories are also found in several areas in The Soppeng district. This paper proposes information for local government to implement health development planning and programs, particularly preventing and treating infectious diseases in Soppeng District.

scholarly journals Comparison of Elisa and Rapid Immunochromatographic Tests in Diagnosis of Toxoplasmosis in Port Harcourt, Nigeria

2020 ◽  
pp. 54-59
Author(s):  
E. O. Onosakponome ◽  
G. N. Wokem ◽  
A. E. Abah
Keyword(s):  
Predictive Value ◽  
Rapid Test ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Efficiency ◽  
Preliminary Screening ◽  
Graphic Data ◽  
Port Harcourt ◽  
Ethical Approval ◽  
Elisa Technique

Toxoplasmosis is a neglected tropical disease with a global distribution that is estimated to infect one third of the world’s human population. This study was a comparison of ELISA and rapid Immunochromatographic tests (ICT) in diagnosis of toxoplasmosis in Port Harcourt Nigeria. Eight hundred patients grouped in four categories from three Health Care Centres were randomly sampled after due ethical approval was obtained. Samples were analysed using Toxo IgG-IgM rapid test (ICT) and Enzyme linked Immunosorbent Assay (ELISA) technique. Socio Demo graphic Data were obtained using well-structured questionnaires. The seroprevalence of toxoplasmosis based on ICT was 28.1% while that of ELISA was 34.5% both significant (P < 0.05) with a relative risk of 0.815. The diagnostic parameters of ICT versus ELISA IgG were sensitively 46.7% specificity 81.7% positive predictive value (PPV) 57.3%, Negative predictive value (NPV) 74.4 with a diagnostic efficiency of 69.6% Cohen Kappas indicate good to moderate agreement between the two tests for detecting IgG. Although ELISA is the gold standard for diagnosing toxoplasmosis, ICT being less expensive, faster with high specificity and good diagnostic efficiency in detecting IgG is recommended as a preliminary screening tool for diagnosing toxoplasmosis in remote areas and facilities because ELISA is laborious, expensive and not readily available.

Predictive value of simple clinical and laboratory variables for dengue hemorrhagic fever in adults

2008 ◽  
Vol 42 (1) ◽  
pp. 34-39 ◽  
Author(s):  
Vernon J. Lee ◽  
David C.B. Lye ◽  
Yan Sun ◽  
Gina Fernandez ◽  
Adrian Ong ◽  
...  
Keyword(s):  
Dengue Hemorrhagic Fever ◽  
Predictive Value ◽  
Hemorrhagic Fever ◽  
Laboratory Variables

scholarly journals COMPARISON OF WIDAL AND TYPHOID IMMUNOGLOBULIN M AND IMMUNOGLOBULIN G IN RAPID AND EARLY DIAGNOSIS OF ENTERIC FEVER

2016 ◽  
Vol 9 (9) ◽  
pp. 243
Author(s):  
Sharanya K ◽  
Vinod K ◽  
Lakshmi K
Keyword(s):  
Blood Culture ◽  
Typhoid Fever ◽  
Immunoglobulin G ◽  
Rapid Test ◽  
High Sensitivity ◽  
Salmonella Typhi ◽  
Clinical Suspicion ◽  
Immunoglobulin M ◽  
Widal Test ◽  
Sensitivity Specificity

ABSTRACTObjective: Typhoid fever is one of the major public health problems in developing countries including India. A simple, reliable, rapid, and earlydiagnostic test has been one of the important needs of the clinicians. The present study was carried out to compare the Widal test and typhoidimmunoglobulin M (IgM) and immunoglobulin G (IgG) rapid test in diagnosing of Salmonella typhi infection.Methods: A total of 100 cases having clinical suspicion of typhoid fever and 40 controls (20 healthy persons and 20 non-typhoidal febrile patients)were studied. Participants were investigated by blood culture, clot culture, Widal test, and typhoid IgM and IgG rapid test, and the results werecompared.Results: Typhoid IgM and IgG test was positive for IgM in 70 cases and IgG for 6 cases of typhoid fever compared to Widal test which showed only58 positive cases. The sensitivity, specificity, positive, and negative predictive value of typhoid IgM was found as 70%, 90%, 94.59%, and 54.55%,respectively. On the other hand, corresponding values for Widal test were 58%, 85%, 90.63%, and 44.74%, respectively.Conclusion: In the present study, the typhoid IgM and IgG yielded remarkable high sensitivity and specificity to diagnose typhoid fever in the firstweek of illness, so it is recommended to use the test in small and less equipped laboratories as a complementary test to Widal.Keywords: Widal, Typhoid immunoglobulin M/immunoglobulin G, Typhoid fever, Blood culture.

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