scholarly journals Comparison of Elisa and Rapid Immunochromatographic Tests in Diagnosis of Toxoplasmosis in Port Harcourt, Nigeria

2020 ◽  
pp. 54-59
Author(s):  
E. O. Onosakponome ◽  
G. N. Wokem ◽  
A. E. Abah
Keyword(s):  
Predictive Value ◽  
Rapid Test ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Efficiency ◽  
Preliminary Screening ◽  
Graphic Data ◽  
Port Harcourt ◽  
Ethical Approval ◽  
Elisa Technique

Toxoplasmosis is a neglected tropical disease with a global distribution that is estimated to infect one third of the world’s human population. This study was a comparison of ELISA and rapid Immunochromatographic tests (ICT) in diagnosis of toxoplasmosis in Port Harcourt Nigeria. Eight hundred patients grouped in four categories from three Health Care Centres were randomly sampled after due ethical approval was obtained. Samples were analysed using Toxo IgG-IgM rapid test (ICT) and Enzyme linked Immunosorbent Assay (ELISA) technique. Socio Demo graphic Data were obtained using well-structured questionnaires. The seroprevalence of toxoplasmosis based on ICT was 28.1% while that of ELISA was 34.5% both significant (P < 0.05) with a relative risk of 0.815. The diagnostic parameters of ICT versus ELISA IgG were sensitively 46.7% specificity 81.7% positive predictive value (PPV) 57.3%, Negative predictive value (NPV) 74.4 with a diagnostic efficiency of 69.6% Cohen Kappas indicate good to moderate agreement between the two tests for detecting IgG. Although ELISA is the gold standard for diagnosing toxoplasmosis, ICT being less expensive, faster with high specificity and good diagnostic efficiency in detecting IgG is recommended as a preliminary screening tool for diagnosing toxoplasmosis in remote areas and facilities because ELISA is laborious, expensive and not readily available.

scholarly journals Dot-ELISA for the diagnosis of neurocysticercosis

2004 ◽  
Vol 46 (5) ◽  
pp. 249-252 ◽  
Author(s):  
Rakhi Biswas ◽  
S.C. Parija ◽  
S.K. Narayan
Keyword(s):  
Predictive Value ◽  
Blood Donors ◽  
Postgraduate Medical Education ◽  
Rapid Test ◽  
Cns Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cysticercus Cellulosae ◽  
Dot Elisa ◽  
Jawaharlal Institute ◽  
And Control

The aim of the present study was to standardize and evaluate dot-Enzyme linked immunosorbent assay (Dot-ELISA), a simple and rapid test for the detection of cysticercus antibodies in the serum for the diagnosis of neurocysticercosis (NCC). The antigen used in the study was a complete homogenate of Cysticercus cellulosae cysts obtained from infected pigs and dotted on to nitrocellulose membrane. Test sera were collected from the patients of NCC, and control sera from patients with other diseases and healthy students and blood donors of the Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) Hospital, Pondicherry, during a study period from 2001 to 2003. Dot-ELISA detected antibodies in 14 of 25 (56%) in clinically suspected cases of NCC, 13 of 23 (56.5%) in CT/MRI proven cases of NCC and 2 of 25 (8%) each in non-cysticercal CNS infection controls and healthy controls. The test showed a sensitivity of 56.25%, specificity of 92%, positive predictive value of 87.09%, and negative predictive value of 70.76%. Results of the present study shows that the Dot-ELISA as a simple test can be used in the field or poorly equipped laboratories for diagnosis of NCC .

Evaluation of the clinical usefulness of a chemiluminometric method for measuring creatine kinase MB

1990 ◽  
Vol 36 (10) ◽  
pp. 1809-1811 ◽  
Author(s):  
J R Pearson ◽  
F Carrea
Keyword(s):  
Creatine Kinase ◽  
Predictive Value ◽  
Characteristic Curve ◽  
High Sensitivity ◽  
High Specificity ◽  
Receiver Operator Characteristic Curve ◽  
Diagnostic Efficiency ◽  
Predictive Values ◽  
Creatine Kinase Mb ◽  
Rule Out

Abstract The use of creatine kinase isoenzymes (CK-MB) in the diagnosis of acute myocardial infarction (AMI) is well established. We evaluated the use of a new chemiluminometric method (CK-Ciba) for measuring CK-MB by calculating its sensitivity, specificity, positive and negative predictive values, and diagnostic efficiency for diagnosing AMI. We tested 633 samples from 229 patients within 4 h of receipt. The patients were divided into four groups: (1) patients who had an AMI, (2) patients who had AMI ruled out, (3) patients who had CK-MB measured for reasons other than to rule out AMI, and (4) patients who had only one sample drawn. Only patients in Groups 1 and 2 were used in the study. AMI was diagnosed by a cardiologist. The prevalence of AMI in our population was 0.18. A receiver-operator characteristic curve was used to establish optimal values for identifying AMI with the CK-Ciba results: CK-MB greater than or equal to 10 micrograms/L and a CK-MB index of greater than or equal to 3.0 (micrograms of CK-MB per U of CK x 100). Using these values, we calculated a sensitivity of 1.00, specificity of 0.97, positive predictive value of 0.87, negative predictive value of 1.00, and a diagnostic efficiency of 0.97. We conclude that the CK-Ciba method has high sensitivity, high specificity, and good predictive values for CK-MB and is appropriate to use to rule out AMI.

scholarly journals Comparison of a New Immunochromatographic Test to Enzyme-Linked Immunosorbent Assay for Rapid Detection of Immunoglobulin M Antibodies to Hepatitis E Virus in Human Sera

2005 ◽  
Vol 12 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Hsiao Ying Chen ◽  
Yang Lu ◽  
Teresa Howard ◽  
David Anderson ◽  
Priscilla Yiquan Fong ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Rapid Detection ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Acute Hepatitis E

ABSTRACT An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of ≤850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries.

scholarly journals Evaluation and Validation of an Enzyme-Linked Immunosorbent Assay and an Immunochromatographic Test for Serological Diagnosis of Severe Acute Respiratory Syndrome

2004 ◽  
Vol 11 (4) ◽  
pp. 699-703 ◽  
Author(s):  
Ming Guan ◽  
Kwok Hung Chan ◽  
J. S. Malik Peiris ◽  
See Wai Kwan ◽  
Siu Yan Lam ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Immunosorbent Assay ◽  
Clinical Symptoms ◽  
Rapid Test ◽  
High Specificity ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Detection Rates ◽  
Specific Antibodies

ABSTRACT A newly developed severe acute respiratory syndrome (SARS)-specific enzyme-linked immunosorbent assay (ELISA) was further validated to confirm cutoff values and evaluate its diagnostic performance with clinical samples. In parallel, an immunochromatographic test was also evaluated. A total of 227 clinical serum specimens collected from SARS patients were used in the study, together with 385 samples from healthy donors. By use of an immunofluorescent (IF) test as the “gold standard, ” both the ELISA and the immunochromatographic test were able to detect immunoglobulin G antibodies to SARS not only from late-convalescent-stage samples (>21 days from the onset of clinical symptoms), as previously established, but also from early-acute-phase samples (1 to 10 days from onset). The ELISA, using an optical density (OD) of 0.25 as its cutoff value, produced the best sensitivity while maintaining high specificity. It detected SARS-specific antibodies in 58, 70, 75, and 95%, respectively, of the four groups of samples collected from patients 1 to 10 days, 11 to 20 days, 21 to 30 days, and more than 30 days after the onset of clinical symptoms. Similarly, the immunochromatographic test detected SARS-specific antibodies in 55, 68, 81, and 79% of the four groups, respectively. The overall specificities for the ELISA and the rapid test were 99.5 and 97.7%, respectively. Although the positive correlation observed between the ELISA OD values and the IF titers was moderate (r = 0.6915; P < 0.001), the detection rates of both the ELISA and the rapid test were found well in agreement with the IF titers.

scholarly journals The Use of Active Melioidosis Detect TM in Early Diagnosis of Melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nur Zahirah

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
Wan Nurliyana Wan Ramli
Keyword(s):  
Positive Predictive Value ◽  
Negative Predictive Value ◽  
Sensitivity And Specificity ◽  
Predictive Value ◽  
Point Of Care ◽  
Rapid Test ◽  
High Specificity ◽  
Culture Method ◽  
Lower Sensitivity ◽  
Spot Urine

Introduction: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently, Active Melioidosis Detect (AMD) have been shown to be useful. Objectives: (1)To measure the sensitivity and specificity of AMD. (2)To study the sensitivity and specificity of early morning urine AMD compared to spot urine AMD. Materials and method: A prospective crosssectional study of clinically suspected melioidosis patients in HTAA and HSNZ from April until December 2018. Blood and urine samples were tested with AMD. Test results were analysed for sensitivity, specificity,positive predictive value and negative predictive value. Results: A total of 89 patients were included in this study.The mean age is 52 years old, and 56.3% were male gender.64% of patients have diabetes mellitus.11 patients have positive blood culture for Bukholderia pseudomallei, 4 of them were tested positive for AMD.3 of them presented with septic shock (3.4%), however none died.The sensitivity of the AMD was 36.4% ([95% CI 12.4 to 68.4]) and the specificity was 66.7% ( [95% CI 46.0 to 82.8]) in all samples, with positive predictive value of 30.7% and negative predictive value of 72%.Blood samples have lower sensitivity of 9.1% ([95% CI 4.8 to 42.9]) with high specificity of 100% ([95% CI 84.5 to 100]). Urine spot samples have higher sensitivity compared to serum and morning urine, with 36.4% ([95%CI 12.4 to 68.4]) and specificity of 88.9% ([95% CI 69.7 to 97.1]). Conclusion: From this pilot study, this test requires further evaluation before incorporating as point of care assay.

scholarly journals UJI CEPAT (RAPID TEST) ANTIBODI IgM TERHADAP Salmonella typhi DEMAM TIFOID

2018 ◽  
Vol 14 (3) ◽  
pp. 109
Author(s):  
Rini Riyant ◽  
Prihatini . ◽  
Siti Rochmatoen
Keyword(s):  
Typhoid Fever ◽  
Dengue Hemorrhagic Fever ◽  
Predictive Value ◽  
Rapid Test ◽  
Hemorrhagic Fever ◽  
Diagnostic Value ◽  
Salmonella Typhi ◽  
Diagnostic Efficiency ◽  
Igm Antibodies ◽  
Health Centres

Typhoid fever is caused by Salmonella typhi. The definitive diagnosis can be made by isolation of Salmonella typhi from blood, bone marrow or other body fluids. To support the clinical diagnosis of typhoid fever in Indonesia, where most hospitals and health centres haveno facilities for cultures, a rapid test for the detection of lipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies was evaluatedon serum samples from patients with typhoid fever. This study is proposed to know the rapid test diagnostic value for the detection oflipopolysaccharides (LPS) Salmonella typhi-specific IgM antibodies. A cross sectional, observational analytical study on 27 typhoidfever and 25 dengue hemorrhagic fever patients of the Dr. Soetomo Hospital, Dr. M Soewandhi General Hospital and Gotong-RoyongClinic has been conducted from January – May 2007. The diagnosis of typhoid fever patients was based on positive gall culture whilethe diagnosis of dengue hemorrhagic fever was based on negative gall culture, positive serology examination for dengue hemorrhagicfever and a recovery from dengue hemorrhagic fever with standard treatment. The sera from patients were examined using a rapid testfor the detection of lipopolysacharides (LPS) Salmonella typhi specific IgM antibodies from Amgenix Onsight of the first blood samples(collected on admission to the hospital) the rapid test for IgM antibodies showed the following: sensitivity 70.4%, specificity 80.0%,positive predictive value 79.2%, negative predictive value 71.4%, diagnostic efficiency 75% respectively. Of the second blood samples(collected 2–3 weeks during the illness) therapid test for IgM antibodies showed the following: sensitivity 88.9%, positive predictive value 82.8%, negative predictive value 87.0%, and diagnostic efficiency 84.6% respectively. The rapid test for IgM antibodies has a high diagnostic value for typhoid fever. The assay uses stabilized components which can be stored at room temperature; the test does notrequire special equipment and may be used in health centres that have no facilities for culture.

scholarly journals Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay and Immunochromatographic Tests for Detection of Immunoglobulin G Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus in SARS Patients

2004 ◽  
Vol 11 (2) ◽  
pp. 287-291 ◽  
Author(s):  
Ming Guan ◽  
Hsiao Ying Chen ◽  
Shen Yun Foo ◽  
Yee-Joo Tan ◽  
Phuay-Yee Goh ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Recombinant Proteins ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Predictive Value ◽  
Rapid Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Kappa Value ◽  
Sensitivity Specificity

ABSTRACT An enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic test for detection of immunoglobulin G (IgG) antibodies in severe acute respiratory syndrome (SARS) patients were developed by utilizing the well-characterized recombinant proteins Gst-N and Gst-U274. The ELISA detected IgG antibodies to SARS-CoV in all 74 convalescent-phase samples from SARS patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. This finding thus led to a kit sensitivity, specificity, and accuracy of 100, 99.5, and 99.6%, respectively. The test thus provided a positive predictive value (PPV) of 98.7% and a negative predictive value (NPV) of 100%. In addition, the ELISA gave a positive delta of 5.4 and a negative delta of 3.6, indicating an excellent differentiation between positives and negatives. The same recombinant proteins were also applied to a newly developed platform for the development of a 15-min rapid test. The resulting rapid test has an excellent agreement of 99.6%, with a kappa value of 1.00, with the ELISA. Again, this rapid test was able to detect 100% of the samples tested (n = 42) while maintaining a specificity of 99.0% (n = 210). The PPV and NPV for the rapid test thus reached 95.3 and 100%, respectively.

Tissue plasminogen activator as a novel diagnostic aid in acute pulmonary embolism

VASA ◽  
2014 ◽  
Vol 43 (6) ◽  
pp. 450-458 ◽  
Author(s):  
Julio Flores ◽  
Ángel García-Avello ◽  
Esther Alonso ◽  
Antonio Ruíz ◽  
Olga Navarrete ◽  
...  
Keyword(s):  
Pulmonary Embolism ◽  
Negative Predictive Value ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Predictive Value ◽  
Acute Pulmonary Embolism ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Utility ◽  
Tissue Plasminogen ◽  
D Dimer

Background: We evaluated the diagnostic efficacy of tissue plasminogen activator (tPA), using an enzyme-linked immunosorbent assay (ELISA) and compared it with an ELISA D-dimer (VIDAS D-dimer) in acute pulmonary embolism (PE). Patients and methods: We studied 127 consecutive outpatients with clinically suspected PE. The diagnosis of PE was based on a clinical probability pretest for PE and a strict protocol of imaging studies. A plasma sample to measure the levels of tPA and D-dimer was obtained at enrollment. Diagnostic accuracy for tPA and D-dimer was determined by the area under the receiver operating characteristic (ROC) curve. Sensitivity, specificity, predictive values, and the diagnostic utility of tPA with a cutoff of 8.5 ng/mL and D-dimer with a cutoff of 500 ng/mL, were calculated for PE diagnosis. Results: PE was confirmed in 41 patients (32 %). Areas under ROC curves were 0.86 for D-dimer and 0.71 for tPA. The sensitivity/negative predictive value for D-dimer using a cutoff of 500 ng/mL, and tPA using a cutoff of 8.5 ng/mL, were 95 % (95 % CI, 88–100 %)/95 % (95 % CI, 88–100 %) and 95 % (95 % CI, 88–100 %)/94 %), respectively. The diagnostic utility to exclude PE was 28.3 % (95 % CI, 21–37 %) for D-dimer and 24.4 % (95 % CI, 17–33 %) for tPA. Conclusions: The tPA with a cutoff of 8.5 ng/mL has a high sensitivity and negative predictive value for exclusion of PE, similar to those observed for the VIDAS D-dimer with a cutoff of 500 ng/mL, although the diagnostic utility was slightly higher for the D-dimer.

D-Dimer Determination to Exclude Pulmonary Embolism: a Two-Step Approach Using Latex Assay as a Screening Tool

1994 ◽  
Vol 72 (01) ◽  
pp. 089-091 ◽  
Author(s):  
P de Moerloose ◽  
Ph Minazio ◽  
G Reber ◽  
A Perrier ◽  
H Bounameaux
Keyword(s):  
Pulmonary Embolism ◽  
Screening Tool ◽  
Screening Tests ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Elisa Technique ◽  
Diagnostic Step ◽  
D Dimer ◽  
Rule Out ◽  
Latex Test

SummaryD-dimer (DD), when measured by a quantitative enzyme-linked immunosorbent assay (ELISA), is a valuable test to exclude venous thromboembolism (VTE). However, DD ELISA technique is not appropriate for emergency use and the available agglutination latex assays are not sensitive enough to be used as an alternative to rule out the diagnosis of VTE. Latex assays could still be used as screening tests. We tested this hypothesis by comparing DD levels measured by ELISA and latex assays in 334 patients suspected of pulmonary embolism. All but one patient with a positive (DD ≥500 ng/ml) latex assay had DD levels higher than 500 ng/ml with the ELISA assay. Accordingly, ELISA technique could be restricted to patients with a negative result in latex assay. This two-step approach would have spared about 50% of ELISA in our cohort. In conclusion, our data indicate that a latex test can be used as a first diagnostic step to rule out pulmonary embolism provided a negative result is confirmed by ELISA and the performance of the latex assay used has been assessed properly.

DETEKSI ANTIBODI MULTIPEL HEPATITIS C DALAM DARAH DONOR

2016 ◽  
Vol 21 (3) ◽  
pp. 261
Author(s):  
Ranti Permatasari ◽  
Aryati Aryati ◽  
Budi Arifah
Keyword(s):  
Hepatitis C ◽  
Predictive Value ◽  
Core Protein ◽  
Rapid Test ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Hcv Infection ◽  
Antibody Detection ◽  
Hcv Rna ◽  
Hcv Antibody

Hepatitis C (HCV) infection could be spread by blood transfusion. Screening of HCV in donor blood could prevent HCV infection to the recipient. HCV antibody test using rapid test of multiple antibody detection by immunochromatography method is an easy and rapid test that could detect four HCV antibodies separately. The aim of this study was to evaluate the diagnostic value of antibody HCV using multiple antibody detection rapid test in diagnosing HCV infection. This was an analytical observational study with a cross sectional design. The samples consisted of 42 donors’ blood serum from the Surabaya Branch of the Indonesian Red Cross which underwent HCV infection test using ELISA method. The samples were then tested using PCR HCV RNA as the gold standard and antibody HCV multiple antibodydetection rapid test The diagnostic value of HCV antibody test using multiple antibody detection rapid test by immunochromatography method showed a diagnostic sensitivity of 100%, diagnostic specificity of 75%, positive predictive value of 66.7% and negative predictive value of 100%, a diagnostic efficiency of 83.3%, with a positive probability ratio of 4 times. The most often positive antibody pattern was four (4) positive antibodies (core protein, NS3, NS4 and NS5). Core protein (CP) and NS3 were the most often positive antibodies. Based on this study result, the HCV antibody test using multiple antibody detection rapid test by immunochromatography method has a good diagnostic value.

Sign in / Sign up

Export Citation Format

Share Document