Comparison of K2 and K3 EDTA Anticoagulant on Complete Blood Count and Erythrocyte Sedimentation Rate

2021 ◽  
Vol 28 (1) ◽  
pp. 75
Author(s):  
Harida Zahraini ◽  
Yulia Nadar Indrasari ◽  
Hartono Kahar
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Complete Blood Count ◽  
Rank Test ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design

The use of anticoagulants is one of the important pre-analytic factors in hematological tests. Both dipotassium (K2) andtripotassium (K3) Ethylene Diamine Tetraacetic Acid (EDTA) are widely used anticoagulants. International CouncilStandardization of Hematology (ICSH) and several researchers recommend the use of K2 EDTA due to its less hyperosmolareffect on blood cells compared to K3 EDTA. This study aimed to compare the results of Complete Blood Count (CBC) andErythrocyte Sedimentation Rate (ESR) using anticoagulant K2 EDTA and K3 EDTA. This study was an analytic observationalstudy with a cross-sectional design conducted from April to December 2018. The subject of the study were 103 healthyadults selected by consecutive sampling. Blood samples were collected in both anticoagulant tubes with a volume of 3 mLeach. Samples were tested twice, in the first 0 hours and the next 6 hours using Sysmex XN 1000 and Alifax Roller 20 LC.Kolmogorov-Smirnov test, paired T-test and Wilcoxon rank test were used for statistical analysis. The agreement testbetween both anticoagulants was carried out using the Bland Altman plot for parameters with a significant difference. Therewas a significant difference between both anticoagulants for the parameters of hemoglobin, hematocrit MCV, MCHC, RDW,PDW, MPV, PLC-R, and erythrocyte sedimentation rate in both the first and second tests. The agreement test using the BlandAltman plot showed that the difference in these parameters was within the Limit of Agreement (LOA) range of 95%. Thisstudy showed that there were differences in some parameters of complete blood count and erythrocyte sedimentation ratebetween the two anticoagulants (K2 K3 EDTA), but these differences were within the LOA range.

scholarly journals A comparison of erythrocyte sedimentation rates of bloods anticoagulated with trisodium citrate and EDTA among TB presumptive patients at the University of Gondar comprehensive specialized hospital, northwest Ethiopia

2020 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Trisodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
The University

Abstract Objective: The purpose of this study was comparing the erythrocyte sedimentation rate (ESR) results of trisodium citrate (TSC) and ethylene diamine tetra-acetic acid (EDTA) anticoagulants. A comparative cross-sectional study was conducted at the University of Gondar specialized referral hospital, northwest, Ethiopia. A total of 70 TB presumptive participants were recruited. From each of the 70 participants of the study, 3 and 1.6 ml of blood was collected in EDTA tubes and 0.4 ml of trisodium Citrate anticoagulant containing test tubes, respectively. Results: The mean ± SD values of ESR were 57.9 ± 41.45 mm/hr in EDTA and 50.99 ± 43.5 mm/hr in TSC anticoagulated blood. The mean difference of ESR values between EDTA and TSC blood (6.91 ± 13.66 mm/hr) was statistically significant. The Mean ± SD of ESR values using EDTA and TSC in males were 59.57 ± 42.31 and 53.57 ± 44.61 mm/hr while for females it was 54.71±40.44 and 46.04 ± 41.82 mm/hr, respectively. The study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants. Keywords: Erythrocyte sedimentation rate, EDTA, Tri-sodium citrate, Westergren Method, Comparison

scholarly journals Comparison of erythrocyte sedimentation rate between blood anticoagulated with sodium citrate and EDTA among Tuberculosis suspected patients at University of Gondar Hospital, North West Ethiopia

2019 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
North West ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
Study Participants

Abstract Objective: The purpose of this study was comparison of erythrocyte sedimentation rate results of sodium citrate and ethylene diamine tetra-acetic acid anticoagulant. A comparative cross-sectional study was conducted at University of Gondar Referral Hospital, Northwest, Ethiopia. A total of 70 TB suspected study participants were recruited. From each study participants, 3ml blood was collected in to EDTA tube and 1.6ml blood was collected in to a test tube containing 0.4ml Sodium Citrate anticoagulant. Results: The mean ± SD values for ESR were 57.9 ± 41.45 in EDTA and 50.99 ± 43.5 in citrate anticoagulated blood. The mean difference of ESR values between EDTA and citrated blood was 6.91 ± 13.66 mm/hr with a significant difference. The mean ± SD of ESR for EDTA and citrate blood was 59.57 ± 42.31 and 54.71 ± 40.44 mm/hr for males and 53.57 ± 44.61 and 46.04 ± 41.82 mm/hr for females, respectively. This study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants.

scholarly journals A comparison of erythrocyte sedimentation rates of bloods anticoagulated with trisodium citrate and EDTA among TB presumptive patients at the University of Gondar comprehensive specialized hospital, northwest Ethiopia

2020 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Trisodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
The University

Abstract Objective: The purpose of this study was comparing the erythrocyte sedimentation rate (ESR) results of trisodium citrate (TSC) and ethylene diamine tetra-acetic acid (EDTA) anticoagulants. A comparative cross-sectional study was conducted at the University of Gondar specialized referral hospital, northwest, Ethiopia. A total of 70 TB presumptive participants were recruited. From each of the 70 participants of the study, 3 and 1.6 ml of blood was collected in EDTA tubes and 0.4 ml of trisodium Citrate anticoagulant containing test tubes, respectively. Results: The mean ± SD values of ESR were 57.9 ± 41.45 mm/hr in EDTA and 50.99 ± 43.5 mm/hr in TSC anticoagulated blood. The mean difference of ESR values between EDTA and TSC blood (6.91 ± 13.66 mm/hr) was statistically significant. The Mean ± SD of ESR values using EDTA and TSC in males were 59.57 ± 42.31 and 53.57 ± 44.61 mm/hr while for females it was 54.71±40.44 and 46.04 ± 41.82 mm/hr, respectively. The study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants. Keywords: Erythrocyte sedimentation rate, EDTA, Tri-sodium citrate, Westergren Method, Comparison

scholarly journals A comparison of erythrocyte sedimentation rate between blood anticoagulated with sodium citrate and EDTA among Tuberculosis suspected patients at University of Gondar Hospital, North West Ethiopia

2019 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
North West ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
Study Participants

Abstract Objective: The main aim of this study was comparison of erythrocyte sedimentation rate results of sodium citrate and ethylene diamine tetra-acetic acid anticoagulant. A comparative cross-sectional study was conducted at University of Gondar Referral Hospital, Northwest, Ethiopia. A total of 70 TB suspected study participants were recruited. From each study participants, 3ml blood was collected in to EDTA tube and 1.6ml blood was collected in to a test tube containing 0.4ml Sodium Citrate anticoagulant. Results: The mean ± SD values for ESR were 57.9 ± 41.45 in EDTA and 50.99 ± 43.5 in citrate anticoagulated blood. The mean difference of ESR values between EDTA and citrated blood was 6.91 ± 13.66 mm/hr with a significant difference. The mean ± SD of ESR for EDTA and citrate blood was 59.57 ± 42.31 and 54.71 ± 40.44 mm/hr for males and 53.57 ± 44.61 and 46.04 ± 41.82 mm/hr for females, respectively. This study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants.

scholarly journals Role of Loop Ileostomy in the Management of Ulcerative Colitis Patients

2020 ◽  
Vol 15 (2) ◽  
pp. 62-68
Author(s):  
Ahmed N. Hussein
Keyword(s):  
Ulcerative Colitis ◽  
Erythrocyte Sedimentation Rate ◽  
Medical Therapy ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Complete Blood Count ◽  
Loop Ileostomy ◽  
Erythrocyte Sedimentation ◽  
Fulminant Ulcerative Colitis

Background: Ulcerative colitis is a chronic inflammatory condition characterized by relapsing and remitting episodes of inflammation limited to the mucosal layer of the colon.Loop ileostomy might be helpful in the management of the disease. Objective: To evaluate the role of loop ileostomy in the management of ulcerative colitis.  Methods: Between Januarys 2008 and January 2018, patients with fulminant ulcerative colitis resistant to medical therapy had been referred to our surgical unit and were included in our study. Assessment of the patients’ medical status (serum electrolytes, total serum protein, complete blood count, erythrocyte sedimentation rate, general stool exam,and abdominal ultrasound and x-rays) and psychological statuswere done and then preoperative status correction followed by defunctioning loop ileostomy operated upon them and followed postoperatively in hospital for the response fortwo weeks of hospitalization. Results: Twenty patients were included in the study, two patients died postoperatively due to comorbidities and pulmonary embolism. Eighteen of twenty patients had a gradual improvement clinically (mucus diarrhea with/without blood, weight gain, fever, psychological and edema) and in hematological and biochemicalvalues (complete blood count, serum albumin protein, erythrocyte sedimentation rate and electrolytes) within two weeks. Conclusion: Defunctioning loop ileostomy is a simple,less morbidprocedure if compared to other medical treatments which can change the complication features of fulminant ulcerative colitis towards either continue in medical therapy or towards definitive curative surgery and cost effective as it decrease the need for expensive medications.

scholarly journals Variation in Early Inflammatory Marker Testing for Infection-Related Hospitalizations in Children

2020 ◽  
Vol 10 (10) ◽  
pp. 851-858
Author(s):  
Jessica L. Markham ◽  
Cary W. Thurm ◽  
Matt Hall ◽  
Samir S. Shah ◽  
Ricardo Quinonez ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Inflammatory Marker ◽  
Diagnostic Testing ◽  
Health Information System ◽  
C Reactive Protein ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Significant Difference

BACKGROUND AND OBJECTIVES: Inflammatory marker testing in children has been identified as a potential area of overuse. We sought to describe variation in early inflammatory marker (C-reactive protein and erythrocyte sedimentation rate) testing for infection-related hospitalizations across children’s hospitals and to determine its association with length of stay (LOS), 30-day readmission rate, and cost. METHODS: We conducted a cross-sectional study of children aged 0 to 17 years with infection-related hospitalizations using the Pediatric Health Information System. After adjusting for patient characteristics, we examined rates of inflammatory marker testing (C-reactive protein or erythrocyte sedimentation rate) during the first 2 days of hospitalization. We used k-means clustering to assign each hospital to 1 of 3 groups on the basis of similarities in adjusted diagnostic testing rates across 12 infectious conditions. Multivariable regression was used to examine the association between hospital testing group and outcomes. RESULTS: We included 55 771 hospitalizations from 48 hospitals. In 7945 (14.3%), there was inflammatory marker testing in the first 2 days of hospitalization. We observed wide variation in inflammatory marker testing rates across hospitals and infections. Group A hospitals tended to perform more tests than group B or C hospitals (37.4% vs 18.0% vs 10.4%; P < .001) and had the longest adjusted LOS (3.2 vs 2.9 vs 2.8 days; P = .01). There was no significant difference in adjusted 30-day readmission rates or costs. CONCLUSIONS: Inflammatory marker testing varied widely across hospitals. Hospitals with higher inflammatory testing for one infection tend to test more frequently for other infections and have longer LOS, suggesting opportunities for diagnostic stewardship.

scholarly journals Perbedaan kadar hemoglobin pada remaja gizi baik yang tinggal di pegunungan dengan yang tinggal di tepi pantai

e-CliniC ◽  
2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Marianne C. Jacobus ◽  
Max F.J Mantik ◽  
Adrian . Umboh
Keyword(s):  
Analytical Study ◽  
Nutrition Status ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Good Nutrition ◽  
The Difference ◽  
Cross Sectional Design ◽  
And Gender ◽  
Main Component

Abstract: Haemoglobin is the main component of red blood cells that serves as a transporter of oxygen and carbon dioxide in the blood. The normal range of haemoglobin values can be used to determine the degree of anemia according to age and gender. Geographical condition such as altitude influences the haemoglobin value. This study aimed to obtain the difference of haemoglobin levels between teenagers with good nutrition status who live at the highland and those at the seaside. This was an observational analytical study with a cross sectional design. Samples were students with good nutrition status of SMPN 3 Tomohon (living at the highland) and those of SMP Kristen Nazaret Tuminting (living at the seaside). There were 60 students who met the inclusion criteria as follows: adolescent, good nutrition status, healthy, aged 13-15 years, lived at the highland or at the seaside ≥ 6 month, and willing to be performed blood examinations, and had been approved by their parents. The Mann-Whitney test for the difference between haemoglobin levels of the two groups showed a p value < 0,001. Conclusion: There was a very significant difference between haemoglobin levels of good nutrition teenagers who lived at the highland and at the seaside. Keywords: haemoglobin levels, good nutrition, teenagers, highland, seaside  Abstrak: Hemoglobin merupakan komponen utama sel darah merah dan berfungsi sebagai transporter oksigen dan karbon dioksida dalam darah. Batas normal nilai hemoglobin dapat digunakan untuk menetapkan derajat anemia, dengan distribusi usia dan jenis kelamin spesifik didasarkan pada sampel referensi sehat. Kondisi geografis, seperti ketinggian tempat dari permukaan laut menjadi faktor pertimbangan dalam distribusi nilai normal hemoglobin. Penelitian ini bertujuan untuk mendapatkan perbedaan kadar hemoglobin antara remaja gizi baik yang tinggal di pegunungan dengan yang tinggal di tepi pantai. Jenis penelitian ini analitik-observasional dengan desain potong lintang. Sampel penelitian yaitu remaja gizi baik yang tinggal di pegunungan yaitu siswa SMPN 3 Tomohon dan yang di tepi pantai yaitu siswa SMP Kristen Nazaret Tuminting. Subjek penelitian berjumlah 60 remaja yang memenuhi kriteria inklusi yaitu gizi baik, sehat, berumur 13-15 tahun, berdomisili di pegunungan atau tepi pantai ≥6 bulan, bersedia dilakukan pemeriksaan darah, dan telah disetujui oleh orang tua. Data yang diperoleh dianalisis menggunakan uji Mann-Whitney dengan bantuan program SPSS. Hasil uji Mann-Whitney mengenai perbedaan kadar hemoglobin antara kedua kelompok menunjukkan nila p <0,001. Simpulan: Terdapat perbedaan yang sangat bermakna kadar hemoglobin remaja gizi baik yang tinggal di pegunungan dengan di tepi pantai.Kata kunci: kadar hemoglobin, remaja gizi baik, pegunungan, tepi pantai

scholarly journals Emotional Intelligence and Self Esteem in Male and Female School Students

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Syeda Razia Bukhari ◽  
Syeda Ishrat Fatima ◽  
Amenah Rashid ◽  
Farhana Saba
Keyword(s):  
Emotional Intelligence ◽  
Economic Status ◽  
Sampling Technique ◽  
Self Esteem ◽  
School Students ◽  
Cross Sectional ◽  
Male And Female ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design

The aim of the research is to investigate that emotional intelligence and self-esteem in male and female school students. The present research design is research cross sectional design.  Sample contained 200 students (100 =male, 100= female)   (mean age = 14.16, SD = 0.740) all belonging to the upper, middle and lower Scio economic status. Whole sample was collected from different schools of Karachi, Pakistan with the help of convenient sampling technique. First, the confidentiality about the participants demographic information was assured after that about the purpose and procedure of research explained to participants. The participants who given consent of participation in research, the demographic form, Urdu version of Rosenberg Self-Esteem Scale (Rosenberg, 1965), and Trait Emotional Intelligence Questionnaire (TEIQue) (K.V. Petrides) were administered on them. Statistical Analysis (t-Test for independent) applied to assess the difference of emotional intelligence and self esteem among male and female.The results reveals there is significant difference among emotional intelligence of male and female school students (t (198) = 6.597, P< .05) and there is also significant difference in self esteem of male and female school students (t (198) = 2.837, P< .05).

scholarly journals Perbedaan Penyembuhan Luka Pasca Ekstraksi Gigi Antara Pasien Perokok Dengan Bukan Perokok Di RSGM Unsrat

e-GIGI ◽  
2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Lidia A. Kewo ◽  
Damajanty H.C. Pangemanan ◽  
Aurelia Supit
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Soft Tissues ◽  
P Value ◽  
Eligibility Criteria ◽  
Cross Sectional ◽  
Inflammatory Phase ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design

Abstract: To date, there are lots of documentations about the adverse effects of smoking on the oral cavity. Albeit, smoking is still considered as a casual thing in our community. Chemicals contained in the cigarette smoke can irritate the gums and soft tissues of the mouth, thus inhibiting wound healing after tooth extraction. This study was aimed to determine the difference in post-extraction dental wound healing between smokers and non-smokers. This was a comparative analytical study with a cross sectional design. Samples were obtained by using total sampling method. Subjects consisted of 16 smokers and 16 non-smokers that fulfilled the study eligibility criteria. Their oral cavities were examined to check the signs of inflammation (calor, dolor, rubor, tumor, and functio laesa). The results showed that there was a difference in post-extraction wound healing in inflammatory phase between smokers and non-smokers. As many as 9.4% of smoker patients and 34.4% of non-smoker patients recovered at 7 days post extraction. The Mann Whitney U test showed a p-value of 0.005. In conclusion, there was a significant difference in post-extraction wound healing between smokers and non-smokers.Keywords: smokers, non-smokers tooth extraction, wound healing Abstrak: Kebiasaan merokok bukan merupakan hal asing di masyarakat walaupun banyak dokumentasi mengenai akibat buruk dari merokok terhadap rongga mulut. Bahan kimia yang terdapat dalam asap rokok dapat mengiritasi gusi dan jaringan lunak mulut sehingga menghambat penyembuhan luka pasca ekstraksi gigi. Penelitian ini bertujuan untuk mengetahui perbedaan penyembuhan luka pasca ekstraksi gigi antara pasien perokok dengan bukan perokok. Jenis penelitian ialah analitik komparatif dengan desain potong lintang. Pengambilan sampel menggunakan total sampling yang memenuhi kriteria penelitian. Terdapat sebanyak 16 orang perokok dan 16 orang bukan perokok sebagai subyek penelitian. Pemeriksaan rongga mulut dilakukan untuk melihat tanda-tanda inflamasi (kalor, dolor, rubor, tumor, dan fungsio laesa). Hasil penelitian menunjukkan terdapat perbedaan penyembuhan luka 7 hari pasca ekstraksi gigi pada fase inflamasi antara pasien perokok dengan yang bukan perokok; sebanyak 9,4% pasien perokok dan 34,4% pasien bukan perokok yang sudah sembuh. Hasil uji Mann Whitney U mendapatkan nilai p=0,005. Simpulan penelitian ini ialah terdapat perbedaan bermakna dalam penyembuhan luka pasca ekstraksi gigi antara pasien perokok dengan yang bukan perokokKata kunci: perokok, bukan perokok, ekstraksi gigi, penyembuhan luka

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