scholarly journals Biophysical parameters of rats’ skin after the administration of methimazole

2014 ◽  
Vol 58 (2) ◽  
pp. 315-319 ◽  
Author(s):  
Marcin Gołyński ◽  
Marcin Szczepanik ◽  
Krzysztof Lutnicki ◽  
Łukasz Adamek ◽  
Magdalena Gołyńska ◽  
...  

Abstract The paper describes the influence of oral administration of methimazole on biophysical skin parameters. Wistar rats of different sex (220-260 g) were used in the experiment. Biophysical skin parameters, such as transepidermal water loss (TEWL), corneometry, and pH were examined at seven-day intervals. Significant changes in the parameters were observed on the 7th d of methimazole administration. The changes were observed in both sex but males appeared to be less sensitive in that respect. Changes in the parameters in the females showed rapid mechanisms, which normalised transepidermal water loss and skin hydration, as well as restored skin barrier functions. TEWL, skin hydration, and skin pH measurements allow an early assessment of skin barrier dysfunction after administration of this drug.

2012 ◽  
Vol 15 (3) ◽  
pp. 553-559 ◽  
Author(s):  
M.P. Szczepanik ◽  
P.M. Wilkołek ◽  
M. Pluta ◽  
Ł.R. Adamek ◽  
Z.J.H. Pomorski

Abstract The purpose of this study was to evaluate transepidermal water loss, skin hydration and skin pH in normal ponies. Sixteen ponies of both sexes were examined in the study. Measurements were taken from seven different sites: the neck region, the shoulder, thorax, lumbar, inguinal, lip region and the auricle. In each of the regions transepidermal water loss (TEWL), skin hydration and skin pH were measured. For transepidermal water loss, the lowest values were observed in the lumbar region (9.71g/hm2), while the highest values were observed in the lip region (22.35 g/hm2). In the case of skin hydration the lowest values were observed for the thorax region (2.13 CU), and the highest for the lip region (41.81 CU). For skin pH, the lowest results were obtained in the lumbar region (6.93), and the highest in the lip region (7.96).


2016 ◽  
Vol 27 (5) ◽  
pp. 428 ◽  
Author(s):  
Yutaka Momota ◽  
Kenichiro Shimada ◽  
Azusa Gin ◽  
Takako Matsubara ◽  
Daigo Azakami ◽  
...  

2015 ◽  
Vol 135 (4) ◽  
pp. 930-935.e1 ◽  
Author(s):  
Maeve Kelleher ◽  
Audrey Dunn-Galvin ◽  
Jonathan O'B. Hourihane ◽  
Deirdre Murray ◽  
Linda E. Campbell ◽  
...  

2005 ◽  
Vol 18 (4) ◽  
pp. 755-760 ◽  
Author(s):  
V. Brazzelli ◽  
T. Barbagallo ◽  
F. Prestinari ◽  
C. Rona ◽  
A. De Silvestri ◽  
...  

Photochemotherapy with psoralen plus ultraviolet A (PUVA) and phototherapy with UVB narrow band (UVB-NB) are used in the treatment of psoriasis. Numerous studies have shown that the additional administration of either topical or systemic antipsoriatic agents may effectively increase the efficacy of these therapies. This study aimed to compare through objective data the efficacy of topical tacalcitol in combination with PUVA or UVB-NB versus PUVA and UVB-NB monotherapy in the treatment of mild to moderate chronic plaque psoriasis. Modified Psoriasis Area and Severity Index (PASI) score, transepidermal water loss (TEWL) and stratum corneum hydration were used to monitor the restoration of skin barrier in the psoriatic plaques of 40 patients during photochemotherapy. The study was a right-left, intra-individual, pre/post comparison trial. PUVA and UVB-NB treatments were given three times a week. On those plaques localized on the right side of the body tacalcitol ointment was applied once a day, in the evening. Corneometry, TEWL and modified PASI score were used to evaluate the response to the treatment at baseline, one month and two months. Thirty-six of the forty enrolled subjects completed the study. The comparison between combination treatments and the PUVA/UVB-NB monotherapy showed no significant differences with regard to modified PASI index. However, significant differences were recorded with regard to TEWL and corneometry. The combination of tacalcitol plus PUVA or tacalcitol plus UVB-NB restored epidermal barrier functions as well as skin hydration faster than PUVA or UVB-NB monotherapy (TEWL: p=0.0050 and corneometry: p=0.003). The combination of tacalcitol plus UVB-NB allowed a better restoration of skin barrier functions than tacalcitol plus PUVA (p=0.013). In conclusion, the combination of tacalcitol plus PUVA or plus UVB-NB improves the therapeutic result. In addition, the data from TEWL and skin hydration suggest a means in which tacalcitol plus UVB-NB induces a better normalization of skin biophysical parameters.


2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Indi Chairunnisa ◽  
Linda Julianti Wijayadi ◽  
Sari Mariyati Dewi Nataprawira

Irritant contact dermatitis (DKI) is a non-immunologic skin inflammation reaction caused by contact with irritants. A janitor is a worker who does a lot of wet work that will repeatedly contact with irritants such as acids, bases, detergents, soap, water, solvents, etc., so that there will be damage to the skin barrier function that starts with loss of the lipid layer and Natural Moisturizing Factor (NMF) so it will reduce skin hydration levels and increase TransEpidermal Water Loss (TEWL). This makes the skin condition becomes dry and the skin defense decreases so that it is easier for DKI. The purpose of this study was to determine the description of skin hydration levels and the incidence of irritant contact dermatitis in janitors at Tarumanagara University. This research is descriptive with a cross-sectional design. A total of 60 people from the janitor became the subject of research. The level of skin hydration is measured by a chronometer. The incidence of DKI in the cleaning staff at Tarumanagara University was 10%, the level of skin hydration in the janitor at Tarumanagara University found hydration levels of dry skin on the right palm (76.7%), left palm (76.7%), back of the hand right (56.7%), and back of the left hand (56.7%). In subjects who experience DKI, levels of hydration of very dry skin on the palm (left-right) and hydration of dry skin on the back of the hand (left-right), and the factors that influence the occurrence of DKI are: gender (female), frequency of washing hands with soap per day, frequency and duration of contact with toilet and floor cleaning products per day and the use of personal protective equipment (PPE), and so it is recommended that cleaners always use PPE when working to prevent DKI and use moisturizers on dry skinABSTRAK:Dermatisis kontak iritan (DKI) adalah suatu reaksi peradangan kulit non-imunologik yang disebabkan oleh kontak dengan bahan iritan. Petugas kebersihan merupakan pekerja yang banyak melakukan pekerjaan basah yang  akan kontak berulang dengan bahan iritan seperti asam, basa, detergen, sabun, air, pelarut, dll, sehingga akan terjadi kerusakan fungsi sawar kulit yang dimulai dengan kehilangan lapisan lipid dan Natural Moisturizing Factor (NMF) sehingga akan menurunkan  kadar hidrasi kulit dan meningkatkan TransEpidermal Water Loss  (TEWL). Hal tersebut membuat kondisi kulit menjadi kering dan pertahanan kulit menurun sehingga lebih mudah terjadi DKI. Tujuan penelitian ini untuk mengetahui gambaran kadar hidrasi kulit dan kejadian dermatitis kontak iritan pada petugas kebersihan di Universitas Tarumanagara. Penelitian ini bersifat deskriptif dengan desain cross sectional. Sejumlah 60 orang dari petugas kebersihan menjadi subjek penelitian. Kadar hidrasi kulit diukur dengan alat korneometer. Angka kejadian DKI pada petugas kebesihan di Universitas Tarumanagara sebesar 10 %, Kadar hidrasi kulit pada petugas kebersihan di Universitas Tarumanagara didapatkan kadar hidrasi kulit  kering pada telapak tangan kanan (76,7%), telapak tangan kiri (76,7%), punggung tangan kanan (56,7%), dan punggung tangan kiri (56,7%). Pada subjek yang mengalami DKI didapatkan kadar hidrasi kulit sangat kering pada telapak tangan(kiri-kanan) dan hidrasi kulit kering  pada punggung tangan (kiri-kanan), dan faktor-faktor yang mempengaruhi kejadian DKI adalah: jenis kelamin (perempuan), frekuensi cuci tangan dengan sabun per hari, frekuensi dan lama kontak dengan produk pembersih toilet maupun lantai per hari dan penggunaan alat pelindung diri (APD), dan sehingga disarankan agar petugas kebersihan selalu memakai APD dengan lengkap saat bekerja untuk mencegah DKI dan menggunakan pelembab pada kulit yang kering


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Johny Bajgai ◽  
Jing Xingyu ◽  
Ailyn Fadriquela ◽  
Rahima Begum ◽  
Dong Heui Kim ◽  
...  

Abstract Background Atopic dermatitis (AD) is a chronic allergic inflammatory skin disease characterized by complex pathogenesis including skin barrier dysfunction, immune-redox disturbances, and pruritus. Prolonged topical treatment with medications such as corticosteroids, calcineurin inhibitors, and T-cell inhibitors may have some potential side-effects. To this end, many researchers have explored numerous alternative therapies using natural products and mineral compounds with antioxidant or immunomodulatory effects to minimize toxicity and adverse-effects. In the current study, we investigated the effects of mineral complex material (MCM) treatment on 2, 4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in SKH-1 hairless mice. Methods Animals were divided into four groups; normal control (NC), negative control treated with DNCB only (DNCB only), positive control treated with DNCB and tacrolimus ointment (PC) and experimental group treated with DNCB and MCM patch (MCM). Skin inflammation and lesion severity were investigated through analyses of skin parameters (barrier score and strength, moisture and trans-epidermal water loss level), histopathology, immunoglobulin E, and cytokines. In addition, reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxidase (GPx), and catalase (CAT) levels were measured in both serum and skin lysate. Results Our results demonstrates that MCM patch improved the progression of AD-like skin lesions by significantly increasing skin barrier strength and decreasing trans-epidermal water loss. Additionally, dermal administration of MCM patch significantly reduced epidermal thickness, ROS, and NO levels in skin lysate. Furthermore, we found that MCM suppressed the levels of AD-involved (Th1 and Th2) cytokines such as IL-2, IFN-γ, and IL-4 in blood. In addition, the levels of other Th1, and Th2 and inflammatory cytokines such as IL-1β, TNF-α, IL-6, IL-12(p70) and IL-10 were found lowest in the MCM group than in the DNCB only and PC groups. Moreover, we found total serum IgE level significantly increased after DNCB treatment, but decreased in the PC and MCM groups. Conclusion Taken together, our findings suggest that MCM application may have beneficial effects either systemic or regional on DNCB-induced AD lesional skin via regulation of the skin barrier function and immune-redox response.


2021 ◽  
Vol 10 (2) ◽  
pp. 359 ◽  
Author(s):  
Trinidad Montero-Vilchez ◽  
María-Victoria Segura-Fernández-Nogueras ◽  
Isabel Pérez-Rodríguez ◽  
Miguel Soler-Gongora ◽  
Antonio Martinez-Lopez ◽  
...  

Multiple diagnostic tools are used to evaluate psoriasis and atopic dermatitis (AD) severity, but most of them are based on subjective components. Transepidermal water loss (TEWL) and temperature are skin barrier function parameters that can be objectively measured and could help clinicians to evaluate disease severity accurately. Thus, the aims of this study are: (1) to compare skin barrier function between healthy skin, psoriatic skin and AD skin; and (2) to assess if skin barrier function parameters could predict disease severity. A cross-sectional study was designed, and epidermal barrier function parameters were measured. The study included 314 participants: 157 healthy individuals, 92 psoriatic patients, and 65 atopic dermatitis patients. TEWL was significantly higher, while stratum corneum hydration (SCH) (8.71 vs. 38.43 vs. 44.39 Arbitrary Units (AU)) was lower at psoriatic plaques than at uninvolved psoriatic skin and healthy controls. Patients with both TEWL > 13.85 g·m−2h−1 and temperature > 30.85 °C presented a moderate/severe psoriasis (psoriasis area severity index (PASI) ≥ 7), with a specificity of 76.3%. TEWL (28.68 vs. 13.15 vs. 11.60 g·m−2 h−1) and temperature were significantly higher, while SCH (25.20 vs. 40.95 vs. 50.73 AU) was lower at AD eczematous lesions than uninvolved AD skin and healthy controls. Patients with a temperature > 31.75 °C presented a moderate/severe AD (SCORing Atopic Dermatitis (SCORAD) ≥ 37) with a sensitivity of 81.8%. In conclusion, temperature and TEWL values may help clinicians to determine disease severity and select patients who need intensive treatment.


Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2021
Author(s):  
Aneta Prokopová ◽  
Jana Pavlačková ◽  
Pavel Mokrejš ◽  
Robert Gál

Chicken stomachs can be processed into collagen hydrolysate usable in cosmetic products. The aim of the study was to verify the effects of a carbopol gel formulation enriched with 1.0% (w/w) chicken hydrolysate on the properties of the skin in the periorbital area after regular application twice a day for eight weeks in volunteers ageed 50 ± 9 years. Skin hydration, transepidermal water loss (TEWL), skin elasticity and skin relief were evaluated. Overall, skin hydration increased by 11.82% and 9.45%, TEWL decreased by 25.70% and 17.80% (always reported for the right and left area). Generally, there was an increase in skin elasticity, a decrease in skin roughness, as the resonance times decreased by 85%. The average reduction of wrinkles was 35.40% on the right and 41.20% on the left. For all results, it can be seen that the longer the cosmetic gel formulation is applied, the better the results. Due to the positive effect on the quality and functionality of the skin, it is possible to apply the cosmetic gel formulation in the periorbital area. The advantage of the product with chicken collagen hydrolysate is also the biocompatibility with the skin and the biodegradability of the formulation.


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