scholarly journals Effects of Feeding Low Protein Diets on Serum and Faeces Parameters in Weaned Piglets

2019 ◽  
Vol 63 (2) ◽  
pp. 37-44 ◽  
Author(s):  
Ľ. Bindas ◽  
L. Bujňák ◽  
I. Maskaľová ◽  
T. Mihok ◽  
P. Timkovičová Lacková ◽  
...  

Abstract This study was conducted to determine the effects of a low-protein diet supplemented with synthetic amino acids on the biochemical parameters in the blood serum, the indicators of fermentation processes, and nitrogen excretion in 12 crossbred piglets. The piglets (weaned at 28 days of age) were divided into two groups with 6 piglets each. The control group had an initial average body weight of 8.8 ± 0.6 kg and the experimental group with an average initial body weight of 8.6 ± 0.7 kg. The control diet contained 210.8 g.kg−1 crude protein and the experimental diet contained 186.4 g.kg−1. The experimental diet was supplemented with lysine, methionine and threonine to achieve a more ideal amino acid pattern. The blood collections from the sinus ophthalmicus for the determination of the biochemical parameters were performed 4 times at weekly intervals in the control and experimental groups 4—5 hours after feeding. The faeces were taken from the rectum at the end of the study period. The decrease in the dietary crude protein content of the experimental group was manifested by a significant decrease of the blood urea level (2.61 mmol.l−1 average concentration) compared to the control groups (4.21 mmol.l−1 average concentration) (P < 0.001). The other serum component concentrations (total protein, albumin, glucose, cholesterol, total lipids and selected enzymes) showed no significant statistical changes between the control and experimental groups. The results of the fermentation process analysis indicated that the butyrate concentration decreased (P = 0.0017) and the pH increased (P = 0.0180) in the experimental group compared to the control group. The levels of crude protein and ammonia in the faeces of experimental animals were significantly lower (P < 0.001) in comparison with those in the control animals.

2000 ◽  
Vol 43 (2) ◽  
pp. 179-192
Author(s):  
H. Nonn ◽  
H. Jeroch

Abstract. Title of the Paper: Investigation on N-reduced feeding and use of free amino acids in fattening pigs The aim of the present study was to investigate the N-reduced feeding by fattening pigs feed by cereals rieh feedmixture supplemented with the free amino-acids L-lysine, DL-methionine, L-threonine, L-tryptophane, Lleucine, L-isoleucine, L-histidine and L-valine. It was to test extreme low crude protein content by requirement sufficient supply on amino-aeid. The reaction of the animals in growth, carcass quality and the reduction of Nexeretion was to investigate. The pigs were divided in two groups (control and experimental group) with 24 animals per group (12 females and 12 castrates). The experiment was divided into three periods (25–60, 60–85 and 85–110 kg body weight). The protein content of the feedmixture was by the control group 18.3, 15.6 and 13.6% and by the experimental group 14.0, 11.8 and 10.7%, respectively. The lysine-energy ratio of the feedmixture was by all three feeding periods by 0.72 or 0.74, 0.61 or 0.62 and 0.55 or 0.57 g lysine per MJ ME in control feedmixture or experimental feedmixture, respectively. By a high Performance are the average daily life weight gain (control group 869 and experimental group 863 g) and the lean meat rate (control group 55.2 and experimental group 55:0%) not influenced by the high of crude protein supply. The calculated N-excretion were by the control group 4.16 kg (as 100%) and by experimental group 2.60 kg (62.5%) per 100 kg body weight gain.


1991 ◽  
Vol 116 (2) ◽  
pp. 303-307 ◽  
Author(s):  
N. Girdhar ◽  
D. Lall ◽  
N. N. Pathak

SUMMARYIn an experiment conducted in the sheds of the Animal Nutrition Division of the Indian Veterinary Research Institute in 1988/89, eight adult Black Bengal goats were divided into two groups of four animals each. Animals in the control group were offered a conventional diet (concentrate and available roughage as per National Research Council standards) and those in the experimental group were fed exclusively on fresh leucaena foliage (young and newly developed leaves and shoots) for 300 days. Dry matter intake (DMI) and body weight of animals in the experimental group decreased in the first 6 weeks; daily DMI/lOOkg body weight was 306 ± 0·34kg in the control group and 2·40 ± 0·18 kg in the experimental group.There were significant differences in the apparent digestibilities of crude protein and nitrogen-free extractives; contents of digestible crude protein (DCP) and total digestible nutrients were 5·2 and 20·3%, respectively, in the controls and 65·6 and 59·1 %, respectively, in the experimental group. DCP intake was significantly higher in the experimental group as a result of high protein content in foliage but it was not well utilized.In the control and experimental groups, respectively, daily mean retention of nitrogen was 2·01±0·14 and 1·54±0·12 g/day, of calcium 1·60±0·08 and 2·92±1·69 g/day, and of phosphorus 1·12±0·25 and 0·24±0·07 g/day, the phosphorus balance being significantly lower in the experimental group. Blood calcium and serum glutamate oxaloacetate transaminase/serum glutamate pyruvate transaminase concentrations were statistically similar in both groups but the concentration of inorganic P was significantly lower in the blood of animals fed leucaena.


2019 ◽  
Author(s):  
O K Gogaev ◽  
A A Abaev ◽  
A R Demurova ◽  
E T Choniashvili

The aim of this research is to study the influence of ozone on the efficiency of incubating quail eggs. The study had been conducted in the period from 2014 to 2018 under the SIE LLC “EcoDom”. In the course of the experiment the Estonian quail egg were served as an object of research. To carry out the experiment a household ozonizer ”Groza” was used. The Eggs of the first “control” group were disinfected with formaldehyde vapors according to the standard method – 35 ml of 37 % formalin solution + 20 ml of tap water + 20 g of potassium permanganate per 1 cubic metre of volume in a special chamber. The other three groups were ozonized. The eggs of the 2nd experimental group were treated for 10 minutes exposure time, the third – 20 minutes and the fourth – 30 minutes, the ozone concentration in all experimental groups was the same and was 10 mg/m3. After treatment, the eggs were placed for brooding. Biochemical parameters of blood serum were determined by the analyzer ”Microlab-300”. Ozonation of hatching eggs contributes to increase crude protein in the quail’s blood serum by 9.37, 9.65 and 8.57 % compared to the control group. Hatching conditional quails was increased by 43.35 % in comparison with the control group.


Author(s):  
Hristo Lukanov, Ivelina Pavlova ◽  
Veselin Ivanov, Todor Slavov, Yoana Petrova, Nadya Bozakova

A total of 90 14-day-old meat type male Japanese quails were included in the experiment and equally divided into 3 groups: control group (CG) without treatment, experimental group 1 (EG 0.5%), supplemented with silymarin 0.5% and experimental group 2 (EG 1%), supplemented with silymarin 1%. The aim of the current study was to establish the effect of supplementation with different doses of silymarin extract on the growth performance and some hematological parameters in male Japanese quails. Main growth performance (body weight, feed consumption and weight gain) were examined and FCR was calculated. Some hematological (WBC, RBC, HGB and HTC) and biochemical (triglycerides, AST and ALT) parameters were determined. The highest average body weight was observed in EG 0.5% group at 35-day-old age and the lowest in EG 1%, 239.61±2.96 g and 231.74±2.96 g respectively. The highest values of the weight gain were calculated in EG 0.5% group, 67.02±2.03 g during the first week of fattening and the lowest was observed in EG 1% group at the fifth week after hatching, 23.30±2.03 g respectively. The lowest values of FCR were observed during the first week of the trial, 2.42±0.08 kg/kg to 2.61±0.13 kg/kg in EG 1% and the control group respectively. The supplementation of 0.5% and 1% silymarin did not significantly change the studied hematological and biochemical parameters. A tendency of higher values of triglycerides and lower values of AST and ALT was observed in the groups, supplemented with silymarin compared to the controls. From the obtained results of the present study we can conclude that the silymarin supplementation at higher doses (0.5% and 1%) did not have effect on the main growth performance and some hematological and biochemical parameters of male Japanese quails.


2014 ◽  
Vol 2 (1) ◽  
pp. 17-21
Author(s):  
Sadia Choudhury Shimmi ◽  
M Tanveer Hossain Parash ◽  
Lazina Afrin ◽  
Pervin Akter

Background: Kidney is the main excretory organ which can be damaged by various disease conditions, foods, exposure to some chemicals, toxins, or infectious agents. Peanuts (Arachis hypogaea) may have antioxidant activity thereby can be used for the improvement of kidney functions though its exact role is yet to be explored. Objective: To observe the effect of peanut kernel powder on kidney by observing the histology and some biochemical parameters (serum creatinine and blood urea) in Wistar albino rats. Materials and method: This experimental study was conducted between October 2012 to December 2012 in the Institute of Food and Nutrition, University of Dhaka, Bangladesh. A total number of 20 apparently healthy Wistar albino male rats, weighing between 120 to 150 grams, age range 90 to 120 days were used. Prior to conducting the study, the animals were acclimatized for 14 days. Then, they were divided into two groups; control group (Group A) consisted of 10 rats and experimental group (Group B- Peanut treated group) consisted of 10 rats. All groups of animals received basal diet for 21 consecutive days and in addition, experimental group received peanut kernel powder (500mg/kg body weight/day; orally) in the morning along with food for 21 consecutive days. All the animals were sacrificed on 22nd day. The blood and kidney samples were collected. Blood urea, serum creatinine levels were measured and histopathology of kidney was done by using standard laboratory procedure. Results: The mean body weight of peanut treated group was significantly lower than that of control group. The mean blood urea and creatinine levels were higher in peanut treated group in comparison to those of control group but the differences were not statistically significant. On histology, kidney revealed normal findings both in control and peanut treated group. Conclusion: Role of peanut kernel powder in normalizing the biochemical parameters is controversial. DOI: http://dx.doi.org/10.3329/dmcj.v2i1.17792 Delta Med Col J. Jan 2014; 2(1): 17-21


Author(s):  
Aaron R. Caldwell ◽  
Megan E. Rosa-Caldwell ◽  
Carson Keeter ◽  
Evan C. Johnson ◽  
François Péronnet ◽  
...  

<b><i>Background:</i></b> Debate continues over whether or not individuals with low total water intake (TWI) are in a chronic fluid deficit (i.e., low total body water) [<xref ref-type="bibr" rid="ref1">1</xref>]. When women with habitually low TWI (1.6 ± 0.5 L/day) increased their fluid intake (3.5 ± 0.1 L/day) for 4 days 24-h urine osmolality decreased, but there was no change in body weight, a proxy for total body water (TBW) [<xref ref-type="bibr" rid="ref2">2</xref>]. In a small (<i>n</i> = 5) study of adult men, there were no observable changes in TBW, as measured by bioelectrical impedance, after increasing TWI for 4 weeks [<xref ref-type="bibr" rid="ref3">3</xref>]. However, body weight increased and salivary osmolality decreased indicating that the study may have been underpowered to detect changes in TBW. Further, no studies to date have measured changes in blood volume (BV) when TWI is increased. <b><i>Objectives:</i></b> Therefore, the purpose of this study was to identify individuals with habitually low fluid intake and determine if increasing TWI, for 14 days, resulted in changes in TBW or BV. <b><i>Methods:</i></b> In order to identify individuals with low TWI, 889 healthy adults were screened. Participants with a self-reported TWI less than 1.8 L/day (men) or 1.2 L/day (women), and a 24-h urine osmolality greater than 800 mOsm were included in the intervention phase of the study. For the intervention phase, 15 participants were assigned to the experimental group and 8 participants were assigned to the control group. The intervention period lasted for 14 days and consisted of 2 visits to our laboratory: one before the intervention (baseline) and 14 days into the intervention (14-day follow-up). At these visits, BV was measured using a CO-rebreathe procedure and deuterium oxide (D<sub>2</sub>O) was administered to measure TBW. Urine samples were collected immediately prior, and 3–8 h after the D<sub>2</sub>O dose to allow for equilibration. Prior to each visit, participants collected 24-h urine to measure 24-h hydration status. After the baseline visit, the experimental group increased their TWI to 3.7 L for males and 2.7 L for females in order to meet the current Institute of Medicine recommendations for TWI. <b><i>Results:</i></b> Twenty-four-hour urine osmolality decreased (−438.7 ± 362.1 mOsm; <i>p</i> &#x3c; 0.001) and urine volume increased (1,526 ± 869 mL; <i>p</i> &#x3c; 0.001) in the experimental group from baseline, while there were no differences in osmolality (−74.7 ± 572 mOsm; <i>p</i> = 0.45), or urine volume (−32 ± 1,376 mL; <i>p</i> = 0.89) in the control group. However, there were no changes in BV (Fig. <xref ref-type="fig" rid="f01">1</xref>a) or changes in TBW (Fig. <xref ref-type="fig" rid="f01">1</xref>b) in either group. <b><i>Conclusions:</i></b> Increasing fluid intake in individuals with habitually low TWI increases 24-h urine volume and decreases urine osmolality but does not result in changes in TBW or BV. These findings are in agreement with previous work indicating that TWI interventions lasting 3 days [<xref ref-type="bibr" rid="ref2">2</xref>] to 4 weeks [<xref ref-type="bibr" rid="ref3">3</xref>] do not result in changes in TBW. Current evidence would suggest that the benefits of increasing TWI are not related changes in TBW.


Author(s):  
I. A. Pushkaryev ◽  
N. M. Kostomakhin ◽  
S. V. Burtseva ◽  
N. A. Novikov

The reproductive traits of sows are of paramount importance in the production of high-quality pork. The world experience of pig farming shows the need to solve first of all the feed problem. Diets unbalanced in the content of vitamins restrain the manifestation of genetically inherent high prolificacy of sows. The purpose of the research was to study the influence of feeding the vitamin feed additive “LipoCar” on the reproductive traits of sows and the biochemical parameters of blood serum. The results of research on the introduction of vitamin feed additive “LipoCar” into the diet of pregnant sows have been presented in the article. The experiment has been carried out in the production conditions in JSC “Linevsky pedigree farm” on sows of Large White breed. Pregnant sows have received a basic diet, balanced for all the normalized elements of nutrition. Sows of the experimental group in the composition of the basic diet fed the feed additive “LipoCar”. The dosage of the drug “Lipocar” was 2,1 g/head/day. “Lipocar” has been fed for 20 days. The total duration of the experiment was 3 months. When introducing the vitamin feed additive “LipoCar” into the main diet of sows in the second half of pregnancy the increase in the number of piglets in the litter by 1,6-6,8 % and the weight of the litter – by 5,6-56,2 % (P ≤ 0,001) have been revealed in comparison with the control group of animals. There was a higher content of carotene in the blood serum of sows of the experimental group by 50,0 % (P ≤ 0,01), vitamin A - by 48,7 % (P ≤ 0,05), and total protein – by 4,9 % (P ≤ 0,05).


Author(s):  
D. Skafar ◽  
D. Shumeyko

Purpose: to study the effect of ethanol on the parameters of THC, the percentage of granulocytes and total protein in the hemolymph of the Red claw crayfish (Cherax quadricarinatus).Materials and methods. The object of this experiment was 26 males of the Australian red-clawed crayfish (Cherax quadricarinatus) weighing from 23 to 83 g. The individuals were evenly divided into two experimental groups - with an injection of ethanol and a control group without an injection of 13 crayfish for each group. The injection dose was 2515 mg per 100 g of body weight. A day after the introduction of ethanol, hemolymph was taken with a syringe from the ventral sinus, the syringe was pre-washed with a 4% EDTA-Na2 solution. Three parameters were determined: the total hemocyte count (THC), percent granulocytes and percent total protein content. Counting of hemocytes and determination of granulocytes were performed in a Goryaev chamber under a light microscope. The total protein was determined by the refractometric method.Results. Differences in THC and total protein between the groups were statistically unreliable (p>0,05). THC in the experimental group is 36% more than in the control group. The total protein after the introduction of ethanol actually increased by 0,7%, and relatively by 14%. There were statistically different indicators of the proportion of granulocytes (p<0,05) - the average value of 33,1% in the experimental group versus 24,5% in the control group. A reliable (p<0,05) strong feedback was revealed between the total protein and the mass of individuals in both experimental groups, while in the experimental group there is a visible shift in the values of dependent hemolymph indicators towards an increase in smaller individuals.Conclusion. A single injection of ethyl alcohol with a dosage of 2515 mg per 100 g of body weight into the hemolymph of C. quadricarinatus does not cause significant changes in the THC and total protein after 24 hours. At the same time, the proportion of granulocytes actually increases by 9%, relative to 37%. This may indicate that granulocytes are involved in the formation of cancer defense mechanisms when exposed to toxic substances. The effect of different dosages of ethanol injections and the duration of its effect on hematological parameters requires additional consideration. It is necessary to investigate its effect on other indicators, such as the pH and buffer capacity of the hemolymph, the concentration of hemocyanin, glucose, lactates and calcium.


2016 ◽  
Vol 51 (9) ◽  
pp. 688-695 ◽  
Author(s):  
Roberto Benis ◽  
Matteo Bonato ◽  
Antonio La Torre

Context: Neuromuscular training enhances unconscious motor responses by stimulating both the afferent signals and central mechanisms responsible for dynamic joint control. Dynamic joint-control training is a vital component of injury-prevention programs. Objective: To investigate the effects of body-weight neuromuscular training on Y-Balance Test (YBT) performance and postural control in female basketball players. Design: Randomized controlled clinical trial. Setting: Basketball practice sessions. Patients or Other Participants: A total of 28 healthy elite female basketball players were randomly assigned to an experimental (n = 14) or a control group (n = 14). Intervention(s): Before their regular practice sessions, the experimental group warmed up with body-weight neuromuscular exercises and the control group with standard tactical-technical exercises twice weekly for 8 weeks. Main Outcome Measure(s): Anterior-, posteromedial-, and posterolateral-reach and composite YBT scores were measured before and after 8 weeks of training. Results: Improvement over baseline scores was noted in the posteromedial (right = 86.5 ± 4.5 cm versus 89.6 ± 2.2 cm, +3.5%, P = .049; left = 85.5 ± 4.3 cm versus 90.2 ± 2.7 cm, +5.5%, P = .038)- and posterolateral (right = 90.7 ± 3.6 cm versus 94.0 ± 2.7 cm, +3.6%, P = .016; left = 90.9 ± 3.5 cm versus 94.2 ± 2.6 cm, +3.6%, P = .011)-reach directions and in the composite YBT scores (right = 88.6% ± 3.2% versus 94.0% ± 1.8%, +5.4%, P = .0004; left = 89.2% ± 3.2% versus 94.5% ± 3.0%, +5.8%, P = .001) of the experimental group. No differences in anterior reach were detected in either group. Differences were noted in postintervention scores for posteromedial reach (right = 89.6 ± 2.2 cm versus 84.3 ± 4.4 cm, +4.1%, P = .005; left = 94.2 ± 2.6 cm versus 84.8 ± 4.4 cm, +10%, P = .003) and composite scores (right = 94.0% ± 1.8% versus 87.3% ± 2.0%, +7.1%, P = .003; left = 94.8% ± 3.0% versus 87.9% ± 3.4%, +7.3%, P &lt; .0001) between the experimental and control groups. Conclusions: Body-weight neuromuscular training improved postural control and lower limb stability in female basketball players as assessed with the YBT. Incorporating neuromuscular training into the workout routines for basketball players may enhance joint awareness and reduce the risk of lower extremity injury.


Author(s):  
Elena Petrovna Miroshnikova ◽  
Julia Vladimirovna Kilyakova ◽  
Sergey Vladimirovich Ponomarev ◽  
Azamat Ersainovich Arinzhanov ◽  
Maria Sergeevna Miroshnikova

The article presents the results of studies of morphological and biochemical blood parameters of carp juveniles when adding the extract Quercus cortex (1, 2, 3 mg/kg of feed), probiotic preparation of Soya-bifidum (0.7 ml/kg of feed), antibiotics Ciprofloxacin hydrochloride in the composition of the drug Antibak 250 (100 mg/kg of feed). Analysis of hematological parameters of carp juveniles showed that adding Quercus cortex extract to the diet at a dosage of 2 mg/kg of feed contributed to increasing SGE by 66%, the average concentration of hemoglobin in the red blood cell by 20% compared to the control group. An increase in the level of leukocytes compared to the control was recorded only in the IV experimental group and made 13% (P≤0.05). An increase in glucose in the groups receiving Quercus cortex extract has been stated: in the I experimental group- by 4.2 times (P≤0.001), in II – by 3.4 (P≤0.001) and in III – by 3.3 (P≤0.001) compared to the control. Increasing glucose concentration indicates active metabolic processes and better stress resistance in the fish body. Iron content in carp blood was higher only in the first experimental group (by 3 times) compared to the control. Increasing activity of alanine aminotransferase was observed in all experimental groups, the highest rate of this enzyme being recorded in the IV experimental group (feed with probiotic additive Soya-bifidum) with a significant excess of control by 48%. Lactate dehydrogenase enzyme activity exceeded the control in all experimental groups, maximum increase (by 67%) was observed in the IV experimental group. Alkaline phosphatase was below the control in all experimental groups, minimum values being registered in the IV experimental group- by 37% less than the control. Indicators of p-amylase in the IV group exceeded the control by 2.6 times, in the V group – by 1.5 times. In the II group p-amylase was lower than the control by 44%, and in the III – by 1.9 times. According to the obtained hematological data, it has been established that including Quercus cortex extract into the diet of carp juveniles is promising due to the positive effect on the fish physiological status, immune status, metabolic processes, hematopoiesis and digestibility of feed.


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