Hematological parameters of carp juveniles in terms of introducing Quercus cortex extract into diet

The article presents the results of studies of morphological and biochemical blood parameters of carp juveniles when adding the extract Quercus cortex (1, 2, 3 mg/kg of feed), probiotic preparation of Soya-bifidum (0.7 ml/kg of feed), antibiotics Ciprofloxacin hydrochloride in the composition of the drug Antibak 250 (100 mg/kg of feed). Analysis of hematological parameters of carp juveniles showed that adding Quercus cortex extract to the diet at a dosage of 2 mg/kg of feed contributed to increasing SGE by 66%, the average concentration of hemoglobin in the red blood cell by 20% compared to the control group. An increase in the level of leukocytes compared to the control was recorded only in the IV experimental group and made 13% (P≤0.05). An increase in glucose in the groups receiving Quercus cortex extract has been stated: in the I experimental group- by 4.2 times (P≤0.001), in II – by 3.4 (P≤0.001) and in III – by 3.3 (P≤0.001) compared to the control. Increasing glucose concentration indicates active metabolic processes and better stress resistance in the fish body. Iron content in carp blood was higher only in the first experimental group (by 3 times) compared to the control. Increasing activity of alanine aminotransferase was observed in all experimental groups, the highest rate of this enzyme being recorded in the IV experimental group (feed with probiotic additive Soya-bifidum) with a significant excess of control by 48%. Lactate dehydrogenase enzyme activity exceeded the control in all experimental groups, maximum increase (by 67%) was observed in the IV experimental group. Alkaline phosphatase was below the control in all experimental groups, minimum values being registered in the IV experimental group- by 37% less than the control. Indicators of p-amylase in the IV group exceeded the control by 2.6 times, in the V group – by 1.5 times. In the II group p-amylase was lower than the control by 44%, and in the III – by 1.9 times. According to the obtained hematological data, it has been established that including Quercus cortex extract into the diet of carp juveniles is promising due to the positive effect on the fish physiological status, immune status, metabolic processes, hematopoiesis and digestibility of feed.

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Feed supplement based on endo- and exometabolites B. subtilis as stimulator of milk production of cows

Veterinaria Kubani ◽

10.33861/2071-8020-2020-4-19-22 ◽

2020 ◽

pp. 19-22

Author(s):

Sergey V. Malkov ◽

◽

Aleksandr S. Krasnoperov ◽

Antonina P. Poryvaeva ◽

Natalya A. Vereshchak ◽

...

Keyword(s):

Bacillus Subtilis ◽

Protein Content ◽

Milk Production ◽

Blood Parameters ◽

Feed Additives ◽

The Body ◽

Control Group ◽

Physiological Status ◽

Milk Productivity ◽

Experimental Group

Promising direction in ensuring food security and independence of Russia is the widespread use and study of new feed additives of probiotic action. Today, probiotic preparations are widely used in dairy farming. They support the positive microbial balance of the gastrointestinal tract and stimulate the activity of the digestive, motor-evacuation, hormonal and immune systems. Authors studied the efficiency of the composition of 2 strains of Bacillus subtilis during 14 days after calving (1 experimental group) and 14 days before calving and 14 days after calving (2 experimental group) in the technology of milk production and improving its quality. In the process of conducting scientific and industrial experience, modern research methods were used that were able to obtain high-quality and most informative data on the processes occurring in the body. Analysis of the results of hematological blood parameters of animals of all groups confirmed the physiological of recovery of the cow's body in the postpartum period, but with different degrees of intensity. Animals of the experimental groups reached peak milk production. By the end of the third month in group 1, milk productivity was 28.94±6.84 kg, in group 2 - 32.17±3.33 kg, which formed an increase of 6.44% and 15.59% relative to the background values. In the control group of cows-27.90±7.25 kg. By day 150, the average daily milk yield in animals of the first group was 25.07 ± 4.38 kg, the second - 25.33 ± 2.52 kg, control 22.75 ± 8.82 kg. Milk quality indicators - fat and protein content in animals receiving probiotic supplements were variable. In the milk of animals of the 1st and 2nd groups, the average fat content was recorded 3.69 g / 100g and 3.74 g / 100g, respectively, and the average protein content was 3.104 g / 100 g and 3.240 g / 100 g. In peers of the control group, the fat and protein content in milk was determined to be 3.79 g / 100 g and 3.016 g / 100 g. The studies established and proved the promise of the widespread introduction of probiotic in dairy cattle breeding, which was based on the composition of endo- and exometabolites of 2 strains of Bacillus subtilis. There was an improvement in the physiological status and an increase in the milk productivity of Holstein cows of the black-motley breed.

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Morphological and biochemical cow blood parameters fed with forest products and an enzyme preparation

BIO Web of Conferences ◽

10.1051/bioconf/20202700073 ◽

2020 ◽

Vol 27 ◽

pp. 00073

Author(s):

Vera Tereshchenko ◽

Olga Ivanova ◽

Evgeny Ivanov ◽

and Lyubov Efimova

Keyword(s):

Enzyme Preparation ◽

Hematological Parameters ◽

Forest Products ◽

Blood Parameters ◽

Biological Properties ◽

Feed Additives ◽

Control Group ◽

Group 2 ◽

Experimental Group ◽

Group 1

In order to improve the productivity of lactating cows and preserve their health, scientists and practitioners are searching for complex feed additives that have valuable biological properties. An experiment aimed to study the effect of feeding coniferous flour, pine nutshell, arabinogalactan, and enzyme preparation Amilosubtilin G3x on the biochemical and hematological parameters was conducted in the LLC “Plemzavod “Taezhny” of the Krasnoyarsk region. According to the principle of analogs, three groups of dairy black-motley cows including 15 animals were formed. The experiment lasted 100 days. The diet for cows of the control group was basic, experimental group 1 was fed with coniferous flour (50 g / goal / day), cedar nutshell (50 g / goal / day), Amilosubtilin G3x (5 g / goal / day), cows of experimental group 2 were fed with coniferous flour (50 g / goal / day), pine nutshell (50 g / goal / day), Amilosubtilin G3x (5 g / goal / day), and arabinogalactan (5 g / goal / day). It was found that the additives did not have a negative effect on the metabolism, but improved blood counts. In the blood of experimental group 2, the concentration of total protein was higher by 2.7% and 2.0%, albumin by 3.0 and 1.0%, calcium by 1, 5 and 6.3%, phosphorus by 10.2 and 10.7%, creatinine by 6.0 and 16.5%, glucose by 4.0 and 2.8%, triglycerides by 11.1% in comparison with the control group and experimental group 1, respectively. The results obtained indicate that the additives can be used for feeding cows.

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The effect of ethanol on some hematological parameters of the australian red claw crayfish (Cherax quadricarinatus)

Genetika i razvedenie zhivotnyh ◽

10.31043/2410-2733-2021-4-85-91 ◽

2022 ◽

pp. 85-91

Author(s):

D. Skafar ◽

D. Shumeyko

Keyword(s):

Body Weight ◽

Total Protein ◽

Hematological Parameters ◽

Group Versus ◽

Control Group ◽

Total Protein Content ◽

Cherax Quadricarinatus ◽

Total Hemocyte Count ◽

Red Claw Crayfish ◽

Experimental Group

Purpose: to study the effect of ethanol on the parameters of THC, the percentage of granulocytes and total protein in the hemolymph of the Red claw crayfish (Cherax quadricarinatus).Materials and methods. The object of this experiment was 26 males of the Australian red-clawed crayfish (Cherax quadricarinatus) weighing from 23 to 83 g. The individuals were evenly divided into two experimental groups - with an injection of ethanol and a control group without an injection of 13 crayfish for each group. The injection dose was 2515 mg per 100 g of body weight. A day after the introduction of ethanol, hemolymph was taken with a syringe from the ventral sinus, the syringe was pre-washed with a 4% EDTA-Na2 solution. Three parameters were determined: the total hemocyte count (THC), percent granulocytes and percent total protein content. Counting of hemocytes and determination of granulocytes were performed in a Goryaev chamber under a light microscope. The total protein was determined by the refractometric method.Results. Differences in THC and total protein between the groups were statistically unreliable (p>0,05). THC in the experimental group is 36% more than in the control group. The total protein after the introduction of ethanol actually increased by 0,7%, and relatively by 14%. There were statistically different indicators of the proportion of granulocytes (p<0,05) - the average value of 33,1% in the experimental group versus 24,5% in the control group. A reliable (p<0,05) strong feedback was revealed between the total protein and the mass of individuals in both experimental groups, while in the experimental group there is a visible shift in the values of dependent hemolymph indicators towards an increase in smaller individuals.Conclusion. A single injection of ethyl alcohol with a dosage of 2515 mg per 100 g of body weight into the hemolymph of C. quadricarinatus does not cause significant changes in the THC and total protein after 24 hours. At the same time, the proportion of granulocytes actually increases by 9%, relative to 37%. This may indicate that granulocytes are involved in the formation of cancer defense mechanisms when exposed to toxic substances. The effect of different dosages of ethanol injections and the duration of its effect on hematological parameters requires additional consideration. It is necessary to investigate its effect on other indicators, such as the pH and buffer capacity of the hemolymph, the concentration of hemocyanin, glucose, lactates and calcium.

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RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i1.15399 ◽

2016 ◽

Vol 9 (1) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

V. S. K. Nishihira ◽

N. J. Mezzomo ◽

M. D. Baldissera ◽

R. A. Vaucher ◽

C. G. Pinto ◽

...

Keyword(s):

Inclusion Complex ◽

Oral Administration ◽

Biochemical Parameters ◽

Wistar Rats ◽

Metabolic Diseases ◽

Hematological Parameters ◽

Density Lipoprotein ◽

Blood Parameters ◽

Control Group ◽

Scanning Calorimetry

Objective: The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.Methods: The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.Results: The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P>0.05) similar to the control group.Conclusion: The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.

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Assessment of Gut Microbiota and Selected Blood Parameters in Weaned Piglets Following Supplementation with a Probiotic and Vitamin C

Annals of Animal Science ◽

10.2478/aoas-2019-0052 ◽

2020 ◽

Vol 20 (1) ◽

pp. 179-189

Author(s):

Leszek Tymczyna ◽

Beata Trawińska ◽

Marta Kowaleczko ◽

Anna Chmielowiec-Korzeniowska ◽

Jerzy Lechowski

Keyword(s):

Vitamin C ◽

Gut Microbiota ◽

Total Cholesterol ◽

Hdl Cholesterol ◽

Blood Parameters ◽

Control Group ◽

Weaned Piglets ◽

E Coli ◽

Experimental Group ◽

Control Group Group

AbstractThe aim of the study was to assess the gut microbiota and selected haematological and biochemical blood parameters of weaned piglets following dietary supplementation with a probiotic and vitamin C. the piglets were divided into a control group (group C) and an experimental group (group E), with 30 piglets in each group. All animals received the same feed ad libitum. The animals in the control group (group C) received feed with no added probiotic and vitamin C. the piglets in the experimental group (group E) were given a supplement containing a probiotic (Bacillus cereus 1×109 CFU/kg) in the amount of 1.5 g/piglet/day and vitamin c in the amount of 300 mg/piglet/day. The supplement was administered for 28 days. The total numbers of bacteria of the family Enterobacteriaceae and of the genus Lactobacillus were determined in faeces. The erythrocyte count, haemoglobin level, haematocrit, leukocyte count, and percentages of neutrophils and lymphocytes were determined in the blood. The biochemical analysis concerned the concentration of triacylglycerols, total cholesterol, and LDL and HDL cholesterol. In group E a significant decrease (P<0.01) in the total number of Enterobacteriaceae bacteria was observed in the faeces of the piglets, accompanied by an increase in the number of lactobacilli relative to group C. E. coli was found to predominate over other microorganisms. Salmonella choleraesuis bacteria were present in the faeces of both groups before administration of the supplement, but were not found after its use. The supplement with probiotic and vitamin C caused a significant increase in the erythrocyte, haemoglobin and haematocrit levels in the blood of the piglets and a significant decrease in the concentration of triacylglycerols, total cholesterol and LDL cholesterol.

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Impact complexes nanokarboksylativ and drug Kvatronan-Se on hematological parameters of blood of experimental animals

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7427 ◽

2017 ◽

Vol 19 (74) ◽

pp. 123-126

Author(s):

N. B. Seba ◽

M. A. Khomenko

Keyword(s):

Blood Cells ◽

Hematological Parameters ◽

Live Weight ◽

Control Group ◽

Experimental Animals ◽

Group I ◽

Control Research ◽

Research Experiment ◽

The Village ◽

Experimental Group

In this paper present the results of research of influence of the preparation «Kvatronan-Se» and carboxylates of Se, Cu, Mn, Cr, Ge in different combinations on haematological parameters of blood of experimental animals. Study was carried out on the basis of PC Haleks-Agro. It is located in the village Gul'sk of Novograd Volynskiy district of Zhytomyr region. In the farm to bred of Simmental cows. For the experiment was formed four groups. We took into account age, live weight of 650–700 kg and the average milk yield 5000–6000 kg. Animals is located in identical conditions of feeding and maintenance. Research experiment was carried out on animals couples counterparts. Preparations introduced animals three times at 10–12 days after insemination. The cows first experimental group were injected complex nanokarboksylativ Ge, Cu, Mn and Cr, second – drug Kvatronan–Se and animals third experimental group was administered a complex composed of Se, Cu, Mn, Cr. On 13 day after insemination in experimental animals were taken blood from tail vein and examined the contents of hematological parameters. Established that injection nanokarboksylativ enhance the fertility of cows in the first and third group, the figure rose to 20% in the second by 40% compared with the control. Research hematological parameters blood animals shows that after entering content drugs of blood cells had minor changes but remained within the physiological norm. The results show that in concentration monocyte the second group was at 5.53% (P > 0.01) higher compared to the control group and 2.3% compared to the first and third. The content of lymphocytes in this group decreased by 26.3% (P > 0.05), 22% and 16% relative to the control, the first and third groups. Also, it should be noted that the hemoglobin in the experimental groups decreased in group I by 7.9% in the II – 3.9% and III by 7.3% compared with the control group. Also, these groups distinguished downward trend eosinophils 1.8%, 1.5%, 2.05%. Then, as the number of segment-nuclear neutrophils contrast, in the first group increased by 18.2% in the second and third at 16.9% comparatively control. The number of monocytes in the second group doslfidniy the suspicion was higher by 66.3% and 0.3% higher than the physiological norm – it may indicate a high immune response.

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The role of rhaponticum carthamoides in feeding of dairy cows

Siberian Herald of Agricultural Science ◽

10.26898/0370-8799-2019-5-7 ◽

2019 ◽

Vol 49 (5) ◽

pp. 52-60

Author(s):

V. A. Voloshin ◽

D. A. Matolinets ◽

N. A. Morozkov ◽

G. P. Maysak

Keyword(s):

Dairy Cows ◽

Dry Matter ◽

Positive Impact ◽

Sugar Content ◽

The Body ◽

Metabolic Energy ◽

Control Group ◽

Metabolic Processes ◽

Rhaponticum Carthamoides ◽

Experimental Group

The results of studies of the use of rhaponticum carthamoides for feeding dairy cows are presented. The experiment was carried out on Holstein cows of black-and-white breed in Perm Territory. Vitaminherbal fl our from green mass of rhaponticum carthamoides at a dose of 0.4 and 1.0 kg per head per day in the period of 10-16 days before calving and in the fi rst 30 days of lactation was introduced into the feeding rations of two experimental groups of cows. High sugar content in rhaponticum carthamoides (from 4.15 to 11.2% in dry matter and metabolic energy from 10.87 to 12.12 MJ/ kg) was noted. In the studied material from this plant, the content of the 20-hydroxyecdysone was 0.49% of active ingredients in the dry matter of the product at a rate of 0.25%. The introduction of rhaponticum carthamoides in the diet of feeding animals contributed to the increase in the intensity of metabolic processes in the body. A gradual increase in cholesterol levels in all experimental groups was noted. The most signifi cant growth was observed in cows of the second experimental group by 0.82 mmol / l or 29.50% (p <0.01). Compared with the fi rst experimental group, the increase was 0.13 mmol / l or 5.2%, and the control group – 0.36 mmol / l or 14.17%. Stimulation of metabolic processes in the body of cows of the experimental groups had a positive impact on reproduction, which was manifested in the reduction of the service period duration, compared with the control group.

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Influence of the feed additive Biovitel on the morphological and biochemical parameters of the blood of steers when intensive fattening technology

Kormlenie sel'skohozjajstvennyh zhivotnyh i kormoproizvodstvo (Feeding of agricultural animals and feed production) ◽

10.33920/sel-05-2109-06 ◽

2021 ◽

pp. 58-65

Author(s):

I. A. Kaluga ◽

R. R. Fatkullin

Keyword(s):

Biochemical Parameters ◽

Hematological Parameters ◽

Correlation Coefficients ◽

Live Weight ◽

Farm Animals ◽

Feed Additive ◽

Physiological Status ◽

Animal Blood ◽

Feed Protein ◽

Experimental Group

One of the most important factors for increasing efficiency is the organization of complete ration feeding is to provide farm animals with all the necessary micro- and macroelements. In this regard, rations should be developed on the basis of detailed norms, taking into account the actual chemical composition and nutritional value of the feed used. The purpose of the research was to study the influence of the feed additive Biovitel on the morphological and biochemical parameters of the blood of steers of Hereford breed under intensive fattening technology. On the base of laboratory blood tests it was possible to trace the effect of the feed protein additive Biovitel on the morphological and biochemical parameters of the blood of experimental animals. The correlation of hematological parameters and productivity of animals when using the feed protein additive Biovitel has been evaluated. It has been found that the use of the protein feed additive Biovitel for steers during the fattening period is an effective means to reduce production losses and has a positive effect on the morphological and biochemical parameters of animal blood. The obtained data indicate that the blood parameters were higher in the animals of the experimental group throughout the entire period of research, which contributes to the increase in the physiological status of the animal organism. The revealed correlation coefficients of the live weight of steers with the biochemical parameters of the blood of animals have shown the presence of closer correlation coefficients in the experimental group of steers have been received the feed additive Biovitel.

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The effect of drone transport on the stability of biochemical, coagulation and hematological parameters in healthy individuals

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2021-0513 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Desirée Perlee ◽

Klaas Henk van der Steege ◽

Gijs den Besten

Keyword(s):

Hematological Parameters ◽

Blood Parameters ◽

Control Group ◽

Healthy Individuals ◽

Glucose Phosphate ◽

Relevant Effect ◽

Novel Approach ◽

Variation Database ◽

Allowable Error ◽

The Stability

Abstract Objectives Transport of blood tubes is mainly by car or pneumatic transport. The transportation of blood tubes by drones is a novel approach for rapid transportation of blood tubes over long distances. However, limited data on the stability of biochemical, coagulation and hematological parameters is available after transport of blood tubes by drone. Methods To investigate the effect of drone transport on the stability of blood parameters, four test flights were performed. Blood was drawn from 20 healthy individuals and 39 of the most frequently measured blood parameters were compared between 4 groups; immediate measurement (control), late measurement, transport by car and transport by drone. Total Allowable Error (TAE) of the EFLM Biological Variation Database was used to determine the clinical relevance of significant differences. Results The majority of blood parameters were not affected by drone transport. Eight of the measured parameters showed significant differences between all the groups; glucose, phosphate, potassium, chloride, hemoglobin, platelet count, APTT and Lactate dehydrogenase (LD). A clinically relevant increase for LD after transport and a decrease for glucose values in time and after transport compared with the control group was shown. Conclusions Transportation of blood tubes from healthy individuals by drones has a limited clinically relevant effect. From the 39 investigated blood parameters only LD and glucose showed a clinically relevant effect.

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Influence of feed additive Vitartil on the productive traits and biochemical parameters of steers’ blood

Kormlenie sel'skohozjajstvennyh zhivotnyh i kormoproizvodstvo (Feeding of agricultural animals and feed production) ◽

10.33920/sel-05-2108-03 ◽

2021 ◽

pp. 25-32

Author(s):

S. S. Mirgalimova ◽

A. O. Trufanova ◽

R. R. Fatkullin

Keyword(s):

Physiological State ◽

Relative Number ◽

Blood Parameters ◽

The Body ◽

Biologically Active ◽

Feed Additive ◽

Control Group ◽

Vital Functions ◽

Productive Traits ◽

Experimental Group

The physiological state of animals is to a certain extent characterized by hematological indicators, since blood occupies a special place in the body. By transferring nutrients and biologically active substances, the blood performs the general regulation of vital functions of the body. The use of Vitartil as a feed additive in the diets of animals leads to an increase in glycolytic processes and a decrease in the body’s need for oxygen delivery, which contributes to the growth of the animal body. The purpose of the research was to study the changes in blood parameters taking into account the age of Hereford steers against the background of the use of Vitartil feed additive to the main diet and its effect on the productive traits of animals. In order to carry out the experiment two groups of steers-analogs of Hereford breed have been formed at the age of 6 months per 10 heads in each. The difference between the groups was that the steers of the experimental group have been received the feed additive Vitartil in addition to the main diet. The greater yield of the meat part was in the steers of the experimental group in comparison with the herdmates of the control group. This indicator in the experimental group was 78,9 %, while in the control group it was lower by 1,6 %. The animals of the experimental group were superior to their herdmates in terms of meat weight by 1,4 kg or by 8,8 %. The relative number of bones was lower in the experimental group and amounted to 18,4 %, and in the control group 19,2 %. The meat content index was high in the steer have been received the biologically active additive Vitartil and was 4,3, and in the control group 4,0. It has been found that the use of Vitartil feed additive to the main diet of Hereford steers increases the blood content of hemoglobin and glucose, which has a certain effect on the morphological and chemical composition of meat, as well as increases its energy value.

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