Influence Of Iron Sources In The Nutrient Medium On In Vitro Shoot Multiplication And Rooting Of Magnolia And Cherry Plum

AbstractIn this study, the effects of compounds providing Fe in chelated (NaFeEDTA and Fe(III)AC) and non-chelated (FeSO4·7H2O) forms as components of culture media, onin vitroshoot multiplication and rooting ofMagnolia soulangeana‘Alexandrina’,Magnolia grandifloraandPrunus cerasifera‘Nigra’ were comparatively evaluated. Each of the tested chemicals was used as a single Fe source in the basal salt medium. In the stages of shoot multiplication and rooting plant response was scored by biometrical indices (number of shoots, leaves and roots, shoot and root length, percent of rooted plants and root hairs). The occurrence of physiological disorders was estimated by visual observations. In presence of FeSO4, symptoms of chlorosis, hyperhy-dricity, early senescence and specific morphology of roots, suggesting Fe deficiency, were observed. These deteriorations were entirely prevented at the application of Fe chelates of which, in this experimental systems, Fe(III)AC was tested for the first time. The addition of Fe(III)AC positively affected the plant quality to extent comparable to that of NaFeEDTA. The obtained data suggest that both applied Fe chelates are more appropriate than non-chelated Fe form and can be alternatively used in the optimization of nutrient media for micropropagation ofMagnoliaandPrunus cerasiferagenotypes.

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Response of Cisplatin Resistant Skov-3 Cells to [Pt(O,O′-Acac)(γ-Acac)(DMS)] Treatment Revealed by a Metabolomic 1H-NMR Study

Molecules ◽

10.3390/molecules23092301 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2301 ◽

Cited By ~ 12

Author(s):

Federica De Castro ◽

Michele Benedetti ◽

Giovanna Antonaci ◽

Laura Del Coco ◽

Sandra De Pascali ◽

...

Keyword(s):

Ovarian Carcinoma ◽

1H Nmr ◽

Mechanism Of Action ◽

Culture Media ◽

Epithelial Ovarian Carcinoma ◽

Multivariate Statistical ◽

Nmr Study ◽

First Time

The novel [Pt(O,O′-acac)(γ-acac)(DMS)], Ptac2S, Pt(II) complex has recently gained increasing attention as a potential anticancer agent for its pharmacological activity shown in different tumor cell lines, studied both in vitro and in vivo. The mechanism of action of Ptac2S, operating on non-genomic targets, is known to be very different from that of cis-[PtCl2(NH3)2], cisplatin, targeting nucleic acids. In this work, we evaluated the cytotoxicity of Ptac2S on the cisplatin resistant Epithelial Ovarian Carcinoma (EOC), SKOV-3 cells, by the MTT assay. A 1H-NMR metabolomic approach coupled with multivariate statistical analysis was used for the first time for Ptac2S to figure out the biological mechanisms of action of the complex. The metabolic variations of intracellular metabolites and the composition of the corresponding extracellular culture media were compared to those of cisplatin (cells were treated at the IC50 doses of both drugs). The reported comparative metabolomic analysis revealed a very different metabolic profile between Ptac2S and cisplatin treated samples, thus confirming the different mechanism of action of Ptac2S also in the Epithelial Ovarian Carcinoma (EOC), SKOV-3 cells line. In particular, higher levels of pyruvate were observed in Ptac2S treated, with respect to cisplatin treated, cells (in both aqueous and culture media). In addition, a very different lipid expression resulted after the exposure to the two drugs (Ptac2S and cisplatin). These results suggest a possible explanation for the Ptac2S ability to circumvent cisplatin resistance in SKOV-3 cells.

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In vitro conservation strategy for endemic and endangered Himalayan liverwort Stephensoniella brevipedunculata Kashyap (Marchantiophyta)

International Journal of Plant and Environment ◽

10.18811/ijpen.v4i02.10 ◽

2018 ◽

Vol 4 (02) ◽

pp. 81-87

Author(s):

A. K. Asthana ◽

S. D. Tewari ◽

Vishwa Jyotsna Singh ◽

Isha Pathak ◽

Vinay Sahu

Keyword(s):

Culture Media ◽

In Vitro Conservation ◽

Conservation Strategy ◽

Healthy Population ◽

Salt Mixture ◽

Young Thalli ◽

Half Strength ◽

Axenic Cultures ◽

First Time

During the present study an effort has been made to propagate (in-vitro) the endangered and endemic Himalayan liverwort Stephensoniella brevipedunculata Kashyap using different culture media under controlled Laboratory conditions. Axenic cultures of the taxon have been established using tubers as explants. Seven combinations of media with Full Strength Knop’s macronutrients; Half-strength Knop’s macronutrients; Half-strength Knop’s macronutrients + Vitamins; Halfstrength Knop’s macronutrients + 0.2 mg L-1 IBA + 0.1 mg L-1 BAP; Half-strength Knop’s macronutrients + 0.1 mg L-1 Kinetin + 0.1 mg L-1 2,4D; Half-strength Knop’s macronutrients + 0.1 mg L-1 IBA + 0.2 mg L-1 BAP and Hoagland no. 2 basal salt mixture were used for culture. The best growth was observed in the Hoagland no. 2 basal salt mixture medium, in which dichotomously branched young thalli were successfully formed. Subsequently healthy population of culture grown plants has been raised on soil in pots for the first time.

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Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v20i2.6917 ◽

1970 ◽

Vol 20 (2) ◽

pp. 225-231 ◽

Cited By ~ 4

Author(s):

Meenakshi Banerjee ◽

Priyanka Modi

Keyword(s):

Plant Tissue ◽

In Vitro Propagation ◽

Liquid Culture ◽

Culture Media ◽

Shoot Multiplication ◽

Axillary Node ◽

Bacopa Monnieri ◽

Shoot Initiation ◽

Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots. Rooted plantlets were successfully transferred to the field, after acclimation in the net house. Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation D.O.I. 10.3329/ptcb.v20i2.6917 Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

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Optimization of cultivated strawberry micropropagation using а biogenic silica and green-tea-flavonoids-based mechanocomposite

PROCEEDINGS OF V INTERNATIONAL SCIENTIFIC CONFERENCE “CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE” ◽

10.33952/2542-0720-2020-5-9-10-86 ◽

2020 ◽

Author(s):

E.V. Ambros ◽

◽

E.A. Karpova ◽

O.V. Kotsupiy ◽

Yu.G. Zaytseva ◽

...

Keyword(s):

Green Tea ◽

Production Systems ◽

Physiological State ◽

Rice Husks ◽

Basal Salt Medium ◽

Axillary Shoots ◽

Planting Material ◽

Beneficial Action ◽

First Time

For the first time, organogenesis and physiological characteristics of Fragaria ananassa microclones (cvs. ‘Alpha’ and ‘Solnechnaya polyanka’) under the influence of mechanocomposite (MC) based on rice husks amorphous silica and flavonoids of green tea during the multiplication stage in in vitro conditions were studied. The addition of the MC (0.0, 2.5, 5.0 and 10.0 mg·L-1) to the Gamborg- Eveleg’s basal salt medium supplemented with 0.75 mg·L-1 6-benzylaminopurine has shown beneficial action on processes of organogenesis followed by enzymatic, photosynthetic, and hormonal activities of in vitro cultured strawberry plantlets. In both cultivars, the high frequency of proliferation (100 %) and maximum number of axillary shoots increased by 1.8–2.0 times on medium supplemented with 5.0 mg·L-1 MC. The concentrations of 2.5 and 5.0 mg·L-1 MC were optimal for obtaining plantlets with high physiological state in in vitro conditions. The results may be used for the development of production systems for a healthy planting material using biotechnological approaches and recommended for commercial strawberry micropropagation.

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In vitro Regeneration and Callogenesis of Libidibia ferrea

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i430495 ◽

2020 ◽

pp. 14-24

Author(s):

Daniel da Silva ◽

Angela Maria Imakawa ◽

Kamylla Rosas Vieira Guedes ◽

Flávio Mauro Souza Bruno ◽

Paulo de Tarso Barbosa Sampaio

Keyword(s):

Culture Media ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Light Sources ◽

Multiplication Rate ◽

Medicinal Species ◽

Efficient System ◽

Blue Led ◽

Friable Callus

Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

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Efisiensi Mikropropagasi Pisang Kepok Amorang melalui Modifikasi Formula Media dan Temperatur

Jurnal AgroBiogen ◽

10.21082/jbio.v6n2.2010.p91-100 ◽

2016 ◽

Vol 6 (2) ◽

pp. 91

Author(s):

Yati Supriati

Keyword(s):

In Vitro Culture ◽

Incubation Temperature ◽

Culture Media ◽

Shoot Multiplication ◽

Root Induction ◽

Incubation Condition ◽

Growth Environment ◽

Media Formulation ◽

The Media

Micropropagation Efficiency of Banana cv Kepok Amorang through Modifications of Culture Media and Incubation Temperature. Yati Supriati. The budless banana cv Kepok Amorang is potentially commercialized due to its sweet taste and does not have flower bud, hence reduced the potential of being infected by the blood disease pathogen. Enhancement of banana industry needs continuous supplies of large number banana seedlings. In vitro culture enable the production of seedlings in a large scale, uniform, quick. The research aims: (1) to formulate an efficient medium for in vitro multiplication of cv Kepok Amorang shoot, (2) to identify efficient growth environment for in vitro culture of cv Kepok Amorang, and (3) to formulate an efficient culture medium for roots inductions of cv Kepok Amorang. The plant material used was in vitro culture of Kepok cv Amorang, 2 cm in height without leaf and root. The media formulation for shoot multiplication were full strength, half strength, one fourth strength MS media, supplemented with either 1, 3, or 5 ppm IBA. On optimization step, the media tested were MS, Knop, Knop and Heller, Hyponex N, Growmore N, and Rosasol N containing of 1 ppm BA. The explants were incubated in culture room with 8, 12, and 16 hours photoperiod with temperatures 30oC (non air conditioned) and 25oC (air conditioned). The root induction trial was done using MS, Knop, Knop and Heller, Hyponex N, Growmore N, and Rosasol N media containing of 1 ppm and 3 ppm IBA. The results showed that the best medium formula for shoot multiplication was ¼ MS + 1 ppm IBA. The best incubation condition was 16 hours photoperiods at 30oC. The best media for root induction was Hyponex 2 g/l + 1 ppm IBA. This culture method reduced cost by Rp 261.7 per plantlet through efficiency of media formulation and electricity use.

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Micropropagation of Isoplexis chalcantha Svent, O´Shanahan from Mature Plants

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v18i2.3395 ◽

1970 ◽

Vol 18 (2) ◽

pp. 131-137 ◽

Cited By ~ 1

Author(s):

S. Mederos-Molina

Keyword(s):

Culture Medium ◽

Culture Media ◽

Nodal Segments ◽

Axillary Shoots ◽

In Vitro Shoots ◽

Half Strength ◽

Modified Ms Medium ◽

High Degree ◽

First Time

Culture medium requirements for micropropagation of Isoplexis chalcantha was achieved for the first time after high degree of contamination and phenolic exudates were detected and solved. Cultures were established from axillary shoots using juvenile branches collected from this medicinal plant. Most satisfying results were obtained using a solidified and a modified MS medium (NO3- : NH4+ ratios) enriched with ascorbic acid or soluble PVP plus GA3, BAP and NAA. Explants (nodal segments) were used for in vitro shoots multiplication and best results were achieved with modified MS plus BAP and auxins. Vigorous shoots rooted without symptoms in the half-strength modified MS enriched with low concentration of IBA. Key words: Isoplexis chalcantha, axillary shoots, contamination, phenolic exudates, culture media, NO3- : NH4+ ratios D.O.I. 10.3329/ptcb.v18i2.3395 Plant Tissue Cult. & Biotech. 18(2): 131-137, 2008 (December)

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Establishment of an efficient micropropagation system in enhancing rooting efficiency via stem cuttings of apple rootstock M9T337

Horticultural Science ◽

10.17221/106/2020-hortsci ◽

2021 ◽

Author(s):

Jiangli Shi ◽

Zhidan Dong ◽

Chunhui Song ◽

Beiyang Xie ◽

Xianbo Zheng ◽

...

Keyword(s):

Adventitious Roots ◽

Culture Media ◽

Adventitious Shoots ◽

Shoot Multiplication ◽

Vital Role ◽

Commercial Production ◽

Stem Cuttings ◽

Apple Rootstock ◽

Environmental Adaptability

Rootstocks play a vital role in regulating the environmental adaptability and controlling the growth and development of apple trees. M9T337, an excellent apple rootstock widely used in commercial orchards, could confer dwarf tree architectures, early fruiting and suitability for high-density planting. However, the rooting ability of M9T3337 is low when it is vegetatively propagated, and researchers have not yet established an efficient micropropagation system. The present study systematically evaluated the multiplication in adventitious shoots and the in vitro formation of adventitious roots to determine the effects of the culture media and plant growth regulators of M9T337 and a rapid micropropagation system was developed. For the shoot multiplication, the highest multiplication index of 3.93 was obtained on Murashige and Skoog (MS) media supplemented with 2.0 mg/L 6-BA, 0.1 mg/L NAA and 0.3 mg/L GA3 from 12 combinations of 6-BA and NAA. Stronger and taller adventitious shoots were grown on MS supplemented with 1.8 mg/L 6-BA and 0.5 mg/L NAA. The optimal media with 100% rooting was obtained using ½ MS supplemented with 0.3 mg/L IBA or MS supplemented with 0.6 mg/L IBA for the rooting induction, resulting in mean rooting numbers of 13.00 and 11.33, respectively. Additionally, the effect on rooting of adding 0.3 mg/L IBA or not on the 1/2 MS and MS media was compared; the results suggested that an appropriate IBA concentration was the key to successful rooting. The rooted plantlets were acclimatised in a shaded greenhouse with an 84% survival rate. The established micropropagation system could be used for the rapid propagation of M9T337 for commercial production.

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THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i5.322 ◽

2017 ◽

Vol 48 (5) ◽

Author(s):

Al-Obaidy & Khierallah

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

Stevia Rebaudiana ◽

Shoot Multiplication ◽

Dry Weight ◽

Fresh Weight ◽

Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm). Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly. Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g. Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants.

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Optimization of culture conditions for high frequency in vitro shoot multiplication in sugarcane (Saccharum officinarum L.)

Journal of Applied and Natural Science ◽

10.31018/jans.v8i3.1001 ◽

2016 ◽

Vol 8 (3) ◽

pp. 1565-1569

Author(s):

M. K. Sharma ◽

R. S. Sengar ◽

P. Chand ◽

R. Singh ◽

S. Gupta ◽

...

Keyword(s):

High Frequency ◽

Room Temperature ◽

Culture Media ◽

Shoot Multiplication ◽

Saccharum Officinarum ◽

Culture Conditions ◽

In Vitro Plant Regeneration ◽

Early Maturing ◽

Growth Room

Present study deals with the optimization of various culture conditions for initiating high frequency in vitro shoot multiplication in two early maturing high yielding sugarcane genotypes namely Co98014 & Co89003. On the behalf of the findings of this study, it was concluded that the temperature, photoperiod and culture media pH affected the frequency of in vitro shoot multiplication in both sugarcane genotypes at a significant level. In both genotypes high frequency shoot multiplication was recorded at growth room temperature 25ÂºC, 16h/8h light/dark photoperiod and culture media pH 6.0. Genotype Co89003 exhibited highest shoot regeneration and multiplication under various culture conditions. The present study suggests the necessity of investigation of these culture conditions separately upon individual sugarcane genotypes prior to develop efficient in vitro plant regeneration protocol for commercial purposes.

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