scholarly journals The Effect of Trifluralin on Post-in vitro Morphogenesis of Five Genotypes of Head Cabbage (Brassica oleraceae var. capitata)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Oushadee A.J. Abeyawardana ◽  
Iva Viehmannová ◽  
Martin Koudela
Keyword(s):  
Germination Rate ◽  
Root Induction ◽  
Shoot Induction ◽  
In Vitro Morphogenesis ◽  
Protocol Development ◽  
Treatment Conditions ◽  
Brassica Oleraceae ◽  
Head Cabbage ◽  
H 30

Abstract Six genotypes of head cabbage (‘Flexima’, ‘Mutsuma’, ‘Septima’, ‘Zeus’, DC6, ‘Ancoma’) have been studied to find an effective method of obtaining polyploids using trifluralin. The effect of various trifluralin concentrations (0.5, 1.0, 1.5, and 2.0 mg·L−1) and treatment conditions (24 °C/24 h, 24 °C/48 h, 30 °C/24 h, and 30 °C/48 h) on organogenesis of organogenic callus, hypocotyl, and seeds was tested. Ploidy level was detected using a flow cytometer. As a result, low survival of hypocotyls and calli was obtained. The shoots induced from calli and hypocotyls failed to develop quality plantlets and most calli and hypocotyls remained recalcitrant for further differentiation. Treated seeds seemed to be promising objects basing on a considerable percentage of plant regeneration. With the increased concentration of trifluralin and increasing press of treatment conditions, the seed germination rate was significantly reduced. Shoot induction from germinated seeds varied among genotypes and treatments. The root induction was independent of treatments but also reduced considerably compared to the control. Under tested conditions, none of the polyploids resulted. Further studies are required to be successful in protocol development.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.) 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 186-194 ◽  
Author(s):  
Jana Šedivá ◽  
Pavla Zahumenická ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Growth Characteristics ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Medium ◽  
In Vitro Production ◽  
Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

scholarly journals In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)

2014 ◽  
Vol 23 (2) ◽  
pp. 211-219
Author(s):  
Md. Abul Kalam Azad ◽  
Hasnatul Arefin ◽  
Md. Amzad Hossain
Keyword(s):  
Date Palm ◽  
Callus Formation ◽  
Shoot Proliferation ◽  
Phoenix Dactylifera ◽  
Shoot Induction ◽  
In Vitro Morphogenesis ◽  
Shoot Initiation ◽  
Phoenix Dactylifera L ◽  
Young Leaves

After inoculation of young leaves of date palm offshoot required about six months to complete the morphogenesis process. Fourteen weeks were required for embryogenic callus formation under continuous dark condition and nine weeks for shoot initiation (under 16/8 h light/dark). The highest number of explants (80%) produced callus in modified MS containing 5 mg/l 2,4-D + 2 mg/l 2ip. Sixty per cent of explants produced callus in the modified medium containing 5 mg/l 2,4-D + 5 mg/l NAA. while only 50 per cent of the explants formed callus in the same medium when supplemented with only 5 mg/l 2,4-D. The induced calli were transferred to modified MS for shoot proliferation. A combination of two cytokines showed better performance than single ones in shoot induction. The highest percentage (70) of shoot developed in modified MS containing 2 mg/l BAP + 1 mg/l Kn. Forty per cent shoot induction was found in the same medium supplemented with 2 mg/l of BAP. Thirty per cent shoot formed in MS containing 1 mg/l of Kn. The shoots were subcultured at three- four week intervals throughout culture duration. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17522 Plant Tissue Cult. & Biotech. 23(2): 211-219, 2013  (December)

Investigations on the regeneration ability of squash cultivars

2010 ◽  
Vol 58 (2) ◽  
pp. 159-166 ◽  
Author(s):  
E. Kiss-Bába ◽  
S. Pánczél ◽  
K. Simonyi ◽  
G. Bisztray
Keyword(s):  
Genetic Manipulation ◽  
Regeneration Ability ◽  
Root Induction ◽  
Vegetable Crops ◽  
Shoot Induction ◽  
Regeneration System ◽  
Leafy Shoots ◽  
Suitable Combination ◽  
Transformation Methods

Pumpkin, squash and zucchini are important vegetable crops in tropical and temperate regions. The development of genetic transformation methods offers the potential of introducing valuable traits into these crops. An efficient in vitro plant regeneration system is a critical point for genetic manipulation. The regeneration ability of three Cucurbita varieties was tested on Murashige and Skoog medium supplemented with different growth regulators. Cotyledons of all the varieties were cultured to investigate the effect of 2,4-D (0, 1, 2, 3, 4 mg l −1 ) with or without KIN (0, 0.5, 5 mg l −1 ) and of BA (0, 1, 1.2 mg l −1 ) combined with IAA (0, 0.9, 1, 1.2 mg l −1 ), on the efficiency of shoot induction. Abscisic acid (0.26 mg l −1 ABA) was also added to one medium. To find the most suitable combination for shoot induction, cotyledon segments of the three varieties were also cultivated on media with different concentrations of BA (0–1.2 mg l −1 ) and IAA (0–0.9 mg l −1 ). Shoot induction was achieved via organogenesis in the tested varieties. Leafy shoots were transferred to root induction media. Regenerated plantlets with roots were transferred to sterile soil. This is the first report on in vitro regeneration from cotyledon explants of the pumpkin cultivar Nagydobosi and the pattypan squash cultivar Óvári fehér.

scholarly journals In Vitro Propagation and Acclimatization of Dragon Tree (Dracaena draco)

Horticulturae ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 64 ◽  
Author(s):  
Alexis Galus ◽  
Ali Chenari Bouket ◽  
Lassaad Belbahri
Keyword(s):  
Root Induction ◽  
Shoot Induction ◽  
Endangered Tree ◽  
Successful Recovery ◽  
Bud Induction ◽  
Dormant Bud ◽  
One Year ◽  
Dracaena Draco ◽  
Effects Of Media

In this study, an efficient in vitro procedure was developed for bud induction, rooting of developing shoots and greenhouse acclimatization of young plantlets of dragon tree (Dracaena draco). Effects of media (S1 (1 mg/L KIN and 1 mg/L NAA), S2 (3 mg/L KIN and 1 mg/L IAA), S3 (1 mg/L BAP and 2 mg/L IBA) and S4 (1 mg/L BAP and 1 mg/L NAA)) on shoot induction and media (R1 (0 mg/L IBA), R2 (0.5 mg/L IBA), R3 (1 mg/L IBA), and R4 (2 mg/L IBA)) on root induction were examined in order to find optimal plant hormone concentrations for efficient Dracaena draco dormant bud development and subsequent rooting. The best shoot induction and rooting media were S1 and S2, and R3 and R4, respectively. Dormant buds from one-year-old Dracaena draco plants submitted to this in vitro procedure allowed successful recovery of up to 8 individuals per explant used. In vitro grown plants were successfully acclimated in the greenhouse. The potential of this in vitro procedure for multiplication of this endangered tree is discussed in this report.

scholarly journals Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

2012 ◽  
Vol 40 (1) ◽  
pp. 107 ◽  
Author(s):  
Cuiqin YANG ◽  
Yaoguo QIN ◽  
Xin SUN ◽  
Shu YUAN ◽  
Honghui LIN
Keyword(s):  
Shoot Regeneration ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Leaf Base ◽  
Stem Segments

An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate plantlets, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric plantlets after an additional 10 days. The hyperhydric plantlets reversed to normal plantlets when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins.

scholarly journals In vitro regeneration in pointed gourd

2008 ◽  
Vol 32 (3) ◽  
pp. 461-471 ◽  
Author(s):  
MA Malek ◽  
MA Bari Miah ◽  
M AL-Amin ◽  
D Khanam ◽  
M Khatun
Keyword(s):  
Shoot Regeneration ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Best Response ◽  
Pointed Gourd ◽  
Regenerated Plantlets ◽  
Better Than

An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in pointed gourd. Highest percent of shoot regeneration was 93.86 when nodal explants were cultured on MS+2.0 mg/1 BAP. The maximum number of shoots (4.00) per explant was observed in MS + 2.0 mg/1 BAP + 0.3 mg/1 NAA from nodal segment. Among the two explants, nodal segment was found better for shoot regeneration. Female genotypes responded better than the male genotypes for shoot induction and proliferation. Lower nodal segment performed the best shoot regeneration. The best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/1 NAA. The regenerated plantlets were successfully established in prepared earthen soil pot.DOI: http://dx.doi.org/10.3329/bjar.v32i3.548Bangladesh J. Agril. Res. 32(3) : 461-471, September 2007

scholarly journals In vitro regeneration for mass propagation in commercial scale of medicinal plant vitex nigundo L.

1970 ◽  
Vol 18 ◽  
pp. 140-145 ◽  
Author(s):  
Md Abu Hena Mostofa Jamal ◽  
ANM Rubaiyath-Bin Rahman ◽  
Dipak Kumar Paul ◽  
Md Rezuanul Islam
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Commercial Scale

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferation and root induction were recorded after three weeks of inoculation and used for calculation. For each treatment 15 explants were used and all the treatments were repeated thrice. Established plantlets were transplanted in earthen pots under natural conditions and the survival rate was recorded. Results: The most effective surface sterilization treatment has been found 0.1 % HgCl2 for 7 minutes. Highest number of shoot was observed in MS medium supplemented with 3.0 mg/ BAP. It was rooted well in full MS containing 2.0 mg/l IAA. The survival rate was 85 % and propagated plantlets were successfully acclimatized in soil. Conclusion: It was observed that shoot tips are more responsive for micropropagation of Vitex nigundo L . Thus the fruitful utilization of rapid clonal propagation, germplasm conservation and distribution of Vitex nigundo, important medicinal plant of Bangladesh, is possible. Keywords: Vitex nigundo; Medicinal plant; Shoot induction; Micropropagation; Regeneration. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8790 JBS 2010; 18(0): 140-145

scholarly journals Efficient Adventitious Morphogenesis from In vitro Cultures of the Medicinal Plant Cymbopogon schoenanthus

2018 ◽  
Vol 28 (2) ◽  
pp. 147-160 ◽  
Author(s):  
Asmaa Abdelsalam ◽  
Kamal Chowdhury ◽  
Ahmed El Bakry
Keyword(s):  
Methyl Jasmonate ◽  
Root Formation ◽  
De Novo ◽  
Folk Medicine ◽  
Root Induction ◽  
Shoot Induction ◽  
High Concentration ◽  
Constant Concentration ◽  
Cymbopogon Schoenanthus

Cymbopogon schoenanthus subsp. proximus is a medicinal grass, the source of sesquiterpene proximadiol and is well known in folk medicine as renal antispasmodic. The effect of growth regulators, different sugar types, methyl jasmonate and vitamins on de novo organogenesis from seed cultures was studied. Among different concentrations of NAA and BA, 4 mg/l NAA in combination with 0.5 mg/l BA produced 100% callus induction. Both 1.0 and 4.0 mg/l NAA in combination with 0.5 mg/l BA yielded the highest number of shoots. High concentration of sucrose 6% was efficient in root induction from organogenic shoots. Sugar types at a constant concentration of 3% had a significant effect on raising shoot numbers. Different concentrations of methyl jasmonate, biotin and Ca-pantothenate showed a decline in de novo shoot induction, but root formation frequency and numbers were significantly improved with all concentrations used. Plant Tissue Cult. & Biotech. 28(2): 147-160, 2018 (December)

Penggunaan NAA (Napthalene Acetic Acid) dan Kinetin (6- furfurylaminopurine) pada Induksi Tunas Kubis Bunga Putih (Brassica oleraceae L. var. Botrytis) secara in-vitro (Use of NAA (Naphtalene acetic acid) and Kinetin (6-furfurylaminopurine) For In-Vitro Shoot Induction of White Cabbage (Brassica oleraceae L. var. Botrytis)

2018 ◽  
Vol 8 (2) ◽  
pp. 52
Author(s):  
Desmin Tabuni ◽  
Jeany Polii-Mandang ◽  
Wenny Tilaar
Keyword(s):  
Acetic Acid ◽  
Experimental Design ◽  
Factorial Design ◽  
Optimum Concentration ◽  
Shoot Induction ◽  
White Cabbage ◽  
Shoot Number ◽  
Agricultural Faculty ◽  
Brassica Oleraceae

Penggunaan NAA (Napthalene Acetic Acid) dan Kinetin (6-furfurylaminopurine) pada Induksi Tunas Kubis Bunga Putih (Brassicaoleraceae L. var. Botrytis) secara in-vitro(Use of NAA (Naphtalene acetic acid) and Kinetin (6-furfurylaminopurine)For In-Vitro Shoot Induction of White Cabbage (Brassica oleraceae L. var.Botrytis)Desmin Tabuni1*), Jeany Sh. Polii-Mandang1), Wenny Tilaar1)1) Pascasarjana Program Studi Agronomi Unstrat Manado, 95115*Email korespondensi: [email protected] 7 Agustus 2018, diterima untuk dipublikasikan 31 Agustus 2018AbstrakTujuan penelitian ini ialah untuk menentukan konsentrasi zat pengaturtumbuh (ZPT) NAA dan kinetin yang terbaik untuk induksi tunas kubis bungaputih (Brassica oleraceae L. var. Botrytis) secara in vitro. Penelitian inidilaksanakan di Laboratorium Bioteknologi Fakultas Pertanian Universitas SamRatulangi Manado, pada bulan Maret – Oktober 2018. Rancangan penelitianyaitu Percobaan Faktorial dalam Rancangan Acak Lengkap (RAL) yang terdiridari 16 kombinasi perlakuan dan diulang sebanyak 5 kali. Hasil analisis ragammemperlihatkan bahwa perlakuan kinetin 0 ppm menghasilkan tinggi tanamanterbesar, yaitu 5,31cm pada umur 4 minggu setelah kultur (MSK). Perlakuan NAA0 ppm menghasilkan jumlah daun terbanyak pada umur 2 MSK (2,2) danterbanyak pada umur 3 MSK (3,10). Perlakuan kinetin 0 ppm menghasilkanjumlah daun terbanyak pada umur 4 MSK (3,75). Perlakuan kinetin 3 ppmmenghasilkan jumlah tunas terbanyak pada umur 4 MSK (4,95). Jumlah tunasterbanyak pada perlakuan kombinasi NAA 0,3 ppm dan kinetin 2 ppm adalah 1,8pada umur 2 MSK.Kata Kunci : kubis, induksi tunas, NAA dan Kinetin, in vitroAbstractThe aim of this study was to determine the optimum concentration of NAAand kinetin for in vitro shoot induction of white cabbage (Brassica oleraceae L.var. Botrytis). This research was carried out in the Biotechnology Laboratory,Agricultural Faculty, Sam Ratulangi University, Manado in March - October 2018.Experimental design in this study was a Factorial Design in CompletelyRandomized Design that consisted of 16 treatment combinations with 5replication. The results of ANOVA showed that 0 ppm kinetin resulted in thelargest plant height, i.e 5.31cm at 4 weeks after culture. The highest leaf numberswere observed at 0 ppm NAA, i.e 2.2 at 2 weeks after culture and 3.10 at 3weeks after culture. The highest leaf numbers was also observed at 0 ppmkinetin, i.e 3.75 at 4 weeks after culture. The treatment of 3 ppm kinetin resultedin the highest shoot number (4.95) at 4 weeks after culture. The highest shootnumber at combination of 0.3 ppm NAA and 2 ppm kinetin was 1.8 at 2 4 weeksafter culture.Keywords: Cabbage, Shoot Induction, NAA and Kinetin, in vitro

Sign in / Sign up

Export Citation Format

Share Document