Antitumoral and antioxidant effect of essential oils and in vitro antioxidant properties of essential oils and aqueous extracts from Salvia pisidica

Biologia ◽  
2010 ◽  
Vol 65 (6) ◽  
Author(s):  
Aysun Ozkan ◽  
Ayse Erdogan ◽  
Munevver Sokmen ◽  
Saadet Tugrulay ◽  
Orhan Unal
Keyword(s):  
Essential Oils ◽  
Cancer Cells ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Wild Form ◽  
Scavenging Activity ◽  
Aqueous Extracts

AbstractThe aim of the work was to investigate antitumoral effect of essential oils on cancer cells and their possible protective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity. Also, in vitro antioxidant properties of essential oils and aqueous extracts from wild form and cultivated form of Salvia pisidica were compared.We found out that essential oils from wild form (IC50 128, and 85.17 μg /mL) and cultivated form (IC50 115.00 and 74.83 μg/mL) differed in their antitumoral effects on Hep G2 and H1299 cells respectively. As a very important we consider the finding that both essentials oils significantly reduced the cytotoxicity induced by the strong oxidant in cancer cells. We assume that protective effect of essential oils on cancer cells against H2O2 cytotoxicity can be accompanied by their antioxidant action.Antioxidant properties of essential oils and aqueous extract from wild and cultivated S. pisidica were determined by different test systems. In DPPH assay, the weakest radical scavenging activity was exhibited by the aqueous extract from wild form (66.55 ± 1.15 μg/mL). Similarly inhibition of linoleic acid oxidation of aqueous extract from wild and cultivated form (50.93% and 59.66%) was lower than essential oils. In the case of reducing power assay and hydroxyl radical scavenging activity, a similar activity pattern was observed as given in the first two systems. Essential oils from wild and cultivated form had higher reducing power activity (EC50; 100.99 ± 3.0 and 96.87 ± 3.15 μg/mL) and hydroxyl radical scavenging activity (EC50; 15.99 ± 0.95 and 13.01 ± 1.35 μg/mL) than aqueous extracts.

Radical Scavenging Activities of Heracleum aquilegifolium Wight (Apiaceae) Fruit Oils in vitro

2010 ◽  
Vol 65 (11-12) ◽  
pp. 653-659 ◽  
Author(s):  
Subbiah Karuppusamy ◽  
Gurunathan Muthuraja
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Nitric Oxide Radical

The fruits of Heracleum aquilegifolium Wight (Apiaceae) were collected from Western Ghats of the Indian Peninsula. The essential oils were extracted by hydrodistillation. The chemical composition of the essential oils was analysed by gas chromatography and gas chromatography-mass spectrometry (GC-MS). β -Pinene (22.3%), 1,8-cineole (20.3%), and β-phellandrene (12.4%) were the main components of H. aquilegifolium fruit oils. The antioxidant properties of essential oils of H. aquilegifolium were examined by different procedures namely reducing power ability, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide radical scavenging activity, hydrogen peroxide scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, and metal chelating activity. The antioxidant activities were compared with those of synthetic antioxidants and standard drugs such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ascorbic acid, α-tocopherol, curcumin, and quercetin. The study confirmed the possible antioxidant potential of essential oils tested with various in vitro antioxidant methods. The presence of monoterpenes in combination with other components in the oils could be responsible for the activity

scholarly journals Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Vijayakumar Sundaram ◽  
Selvaraju Sadhasivam ◽  
Sivaraj Chandrasekaran ◽  
Raaman Nanjian ◽  
Arjun Pandian
Keyword(s):  
Dermal Fibroblast ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Fibroblast Cell ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Scavenging Activity ◽  
Medicinal Uses

Abstract Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

scholarly journals Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp

2020 ◽  
Vol 10 (17) ◽  
pp. 6007 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wen-Shin Lin ◽  
Jing-Wen Tung ◽  
Ya-Chih Cheng ◽  
Min-Yun Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Jujube Fruit

In this study, the effects of different fruit parts and extraction conditions on the antioxidant properties of jujube (Ziziphus jujuba Mill.) fruit were investigated. Five in vitro antioxidant models and statistical analyses were performed. The results revealed that jujube peel with pulp (peel pulp) exhibited better antioxidant capacity than did seeds. Overall, jujube peel pulp extracted using 50% ethanol at 60 °C exhibited the best antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (0.3 ± 0 mg/mL), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (0.5 ± 0 mg/mL), total phenolic content (38.3 ± 0.4 mg gallic acid equivalent per gram dry weight), total flavonoid content (43.8 ± 0.2 mg quercetin equivalent per gram dry weight), and reducing power (41.9 ± 2.2 mg ascorbic acid equivalent per gram dry weight). The results indicated that jujube peel pulp is a more potential natural antioxidant than seeds.

Heterogeneous Method for Grafting of Syringaldehyde onto Chitosan by Laccase Oxidation

2013 ◽  
Vol 477-478 ◽  
pp. 1340-1344
Author(s):  
Cheng Hua Yu ◽  
Ji Cheng Pei ◽  
Fang Dong Zhang
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Amino Groups

Heterogeneous method for grafting of syringaldehyde (SD) onto chitosan by laccase oxidation, yielding a product with improved antioxidant activities. The results showed that about 1.1% amino groups participated in this reaction. The FTIR showed that the absorption of amino groups were decreased, the absorption of benzene ring structures were disappeared and a new band appeared at 1637 cm-1 corresponding to C=N vibrations that were due to the Schiff base reaction between the chitosan and syringaldehyde. Additionally, the chitosan had insignificant reducing power and DPPH free radical scavenging activity, SD-chitosan had reducing power of A700=0.684, DPPH free radical scavenging activity of 53.2%. SD-chitosan presented improved antioxidant properties.

scholarly journals Radical Scavenging Activity of Edible Fungal Sporocarp Extracts from the Niger Delta Region of Nigeria

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Ita B. N. ◽  
Eduok S. I.
Keyword(s):  
Free Radical ◽  
Niger Delta ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Aqueous Extracts ◽  
Metal Chelating ◽  
Niger Delta Region

The radical scavenging activity of acetone, ethanol and aqueous extracts from Agaricus placomyces, Cantharellus cibarius and Pleurotus ostreatus from the Niger Delta region of Nigeria was determined with their metal chelating and reducing power. The extracts exhibited significant free radical scavenging activities in the DPPH assay (IC50 = 2.11 to 4.37 mg/mL) with the aqueous extract from P. ostreatus being the most potent. Also, the aqueous extracts exhibited better metal chelating abilities and reducing power than the ethanol and acetone extracts. In addition, higher content of total phenolics and flavonoids was found in the aqueous extract of A. placomyces (55.63mg GAE/g) and P. ostreatus (15.55 mgQE/g) respectively, whereas ascorbic acid ranged from 0.33 to 1.22 mgAAE/ in all the extracts. Overall, our results indicate that mushrooms particularly Agaricus placomyces, and Pleurotus ostreatus contain high amount of polyphenolic compounds with significant free radical scavenging abilities, notable metal chelating activities and promising reducing power. These properties indicate that the mushrooms are functional foods with the potential to attenuate oxidative stress associated with free radical damage in biological systems.

scholarly journals In VitroAntioxidant and Cytotoxic Activities ofArnebia benthamii(Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Showkat Ahmad Ganie ◽  
Tanveer Ali Dar ◽  
Rabia Hamid ◽  
Ovais Zargar ◽  
Shayaq Ul Abeer ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Rat Liver Microsomes ◽  
Scavenging Activity

Arnebia benthamiiis a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study,in vitroantioxidant and anticancer activity of different extracts ofArnebia benthamiiwere investigated. Antioxidant potential of plant extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. The highest phenolic content (TPC) of 780 mg GAE/g was observed in ethyl acetate, while the lowest TPC of 462 mg GAE/g was achieved in aqueous extract. At concentration of 700 µg/mL, DPPH radical scavenging activity was found to be highest in ethyl acetate extract (87.99%) and lowest in aqueous extract (73%). The reducing power of extracts increased in a concentration dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation (LPO) on rat liver microsomesin vitro. In addition,Arnebia benthamiiextracts exhibited antioxidant effects on Calf thymus DNA damage induced by Fenton reaction. Cytotoxicity of the extracts (10–100 µg/mL) was tested on five human cancer cell lines (lung, prostate, leukemia, colon, and pancreatic cell lines) using the Sulphorhodamine B assay.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY OF PHYLLODIUM PULCHELLUM L. DESV - AN THREATENED MEDICINAL PLANT

2017 ◽  
Vol 10 (10) ◽  
pp. 282
Author(s):  
Gopal Murugan Velmurugan ◽  
Subramaniam Parvathi Anand
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Threatened Medicinal Plant ◽  
In Vitro Antioxidant Activity

  Objectives: In this study, we determined the in vitro antioxidant capacity of Phyllodium pulchellum of aqueous, ethanol, and chloroform leaf extracts.Methods: In this context, the in vitro antioxidant activity was demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis(3- ethylbenzothiazolone-6-sulfonic acid) (ABTS+) radical scavenging assay, the total antioxidant activity of phosphomolybdenum assay and hydroxyl radical scavenging activity in different leaf extracts of P. pulchellum. The antioxidant activity of the extracts was compared to standard ascorbic acid.Results: All the four methods of antioxidant showed good reducing power and reducing capacity with increasing concentration again taking the ethanol leaf extract to the top position. Remarkable of antioxidant activity was observed in ethanol leaf extract on the hydrogen peroxide scavenging activity with the lowest inhibitory concentration 50 values of (155.40 μg/ml) followed by DPPH (432.90 μg/ml) and ABTS+ (524.40 μg/ml).Conclusion: These results suggest that the leaf of P. pulchellum could be a valuable source of new antioxidant properties, from the above results it seen that this plant exhibits pharmaceutical activity. 

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