Effect of osmotic stress on Myrtus communis germination

Biologia ◽  
2012 ◽  
Vol 67 (1) ◽  
Author(s):  
Hamrouni Lamia ◽  
Souayah Naoufel ◽  
Khouja Larbi ◽  
Rejeb Néjib
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Germination Percentage ◽  
Myrtus Communis ◽  
Intraspecific Variations ◽  
Germination Ability ◽  
Germination Speed ◽  
Petri Dishes ◽  
Genetic Pool ◽  
Polyethylene Glycol 6000

AbstractThe effects of water potential on germination were studied in four provenances of Myrtus from different bioclimatic zones in Tunisia. Water potential between 0 and −10 bars were obtained using polyethylene glycol 6000 (PEG-6000) solutions. Seeds were planted in Petri dishes full of sterile perlite, and kept for one month under 1500 lux light with 16h photoperiod and at 25°C. A low water potential induced a reduction in germination percentage and germination speed. Significant variations between the provenances were found even under control conditions, especially at the speed germination level. The provenances from humid regions were the most susceptible to water stress, and their germination percentage showed significant reductions at −8 bars. These differences in germination ability of provenances might be attributed to intraspecific variations resulting from the effects of natural selection and genetic pool background.

scholarly journals Effects of Water Stress on Germination of Yarrow Populations (Achillea Spp.) From Different Bioclimatic Zones in Iran

2014 ◽  
Vol 68 (1) ◽  
pp. 39-54 ◽  
Author(s):  
Parvin Salehi Shanjani ◽  
Masoumeh Izadpanah ◽  
Mohamad Reza Mohamadpour
Keyword(s):  
Polyethylene Glycol ◽  
Natural Selection ◽  
Water Stress ◽  
Water Potential ◽  
Wild Populations ◽  
Genetic Potential ◽  
Intraspecific Variations ◽  
Germination Ability ◽  
Genetic Pool ◽  
Polyethylene Glycol 6000

ABSTRACT The effects of water potential on germination were studied in 18 wild populations of three yarrow species (Achillea tenuifolia, A. vermicularis and A. filipendulina) from different bioclimatic zones in Iran. Water potential between 0 and -0.6 MPa were obtained using polyethylene glycol 6000 (PEG-6000) solutions. The study of water stress on germination showed that, regardless of the species and the populations, the lowering of the water potential reduced the capacity for germination and early seeding growth. These results indicated a strong genetic potential for drought tolerance during germination within each species. These differences in germination ability of wild populations of each species might be attributed to intraspecific variations resulting from the effects of natural selection and genetic pool background.

Effects of water potential on germination of lettuce, sunflower, and citrus seeds

1969 ◽  
Vol 47 (11) ◽  
pp. 1761-1764 ◽  
Author(s):  
Merrill R. Kaufmann
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Water Potential ◽  
Cellulose Acetate Membrane ◽  
Lettuce Seeds ◽  
Water Potentials ◽  
Citrus Seeds ◽  
Germinating Seeds ◽  
Polyethylene Glycol 6000 ◽  
Selection Of

The effect of nearly constant water potential on germination of citrus, sunflower, and lettuce seeds was studied. Water potential equilibration was achieved by placing soil above a cellulose acetate membrane which was in contact with a solution of polyethylene glycol-6000. Selection of solute potentials in the solution resulted in controlled water potentials in the soil over a range of 0 to −14.9 bars for citrus and 0 to −8.0 bars for sunflower and lettuce. The water stress experienced by germinating seeds in this system is largely the result of a matric effect rather than a solute effect.Citrus seeds germinated at water potentials as low as −4.7 bars and lettuce at −4.1 bars, but sunflower germinated at −8.0 bars. Sunflower germinated as rapidly at −4.1 bars as lettuce at −2.3 bars, both reaching 50% germination at about 8 days. Citrus germinated much more slowly, requiring 26 days at 0 bars.

scholarly journals Pastoral plants for rehabilitation of degraded soil in Tunisia: the case for use of Calicotome villosa and Genista spachiana (Fabaceae)

Biologija ◽  
2017 ◽  
Vol 63 (1) ◽  
Author(s):  
Mechergui Kaouther ◽  
Jaouadi Wahbi ◽  
Khouja Mohamed Larbi
Keyword(s):  
Salt Stress ◽  
Polyethylene Glycol ◽  
Water Stress ◽  
Germination Percentage ◽  
Hydrophilic Polymer ◽  
Degraded Soil ◽  
Germination Characteristics ◽  
Germination Speed ◽  
Degraded Ecosystems ◽  
Mean Time

Calicotome villosa and Genista spachiana are classified as vulnerable in Tunisia and they are of great interest for the  rehabilitation of degraded ecosystems, food for goats and camelids, and for the use of its essential oils in phytotherapy. The aim of this study was to examine the  germination characteristics of both species through analysis of the effects of the salt stress and water stress on germination. We monitored and evaluated different germination characteristics (germination percentage, mean time to germination and germination speed) in the presence of the salt and water stress. Calicotome villosa and Genista spachiana can withstand salinity of up to 15 g/l of salt (31–37% germination at 15 g/l) and also tolerate large doses of PEG6000 (30–40% germination at –1.6 MPa: Polyethylene glycol (PEG) is a hydrophilic polymer). The tolerance of the two species to salinity and water stress allows them to be a source of food for goats and camelids during drought.

Effect of different priming treatments and priming durations on melon germination behavior under suboptimal conditions

2018 ◽  
Vol 3 (1) ◽  
pp. 386-392 ◽  
Author(s):  
J. L. Castañares ◽  
C. A. Bouzo
Keyword(s):  
Polyethylene Glycol ◽  
Low Temperature ◽  
Temperature Stress ◽  
Osmotic Potential ◽  
Germination Percentage ◽  
Control Treatment ◽  
Saline Stress ◽  
Mean Germination Time ◽  
Osmotic Agents ◽  
Polyethylene Glycol 6000

AbstractThe objective of this work was to compare the effect of different priming osmotic agents and durations on melon germination in: 1) low temperature stress; 2) saline stress; and 3) low temperature and saline stress. The osmotic agents were polyethylene glycol 6000, KNO3+K3PO4, CaCl2and NaCl, with -1.5 MPa osmotic potential. Priming durations were 3 and 6 days (d). Germination percentage (GP) and mean germination time (MGT) were measured. At 12°C the best GP was 14% with CaCl2/3-d, without germination in control. At 25°C the best GP was 100% with CaCl2/3-d. MGT was reduced one day. At -0.7 MPa GP was 100% with CaCl2/3-d and NaCl 3d. At -1.0 MPa the best GP were 46 and 50% for 3d with NaCl and CaCl2respectively without germination in control treatment. At 12°C and -1.0 MPa the best GP were CaCl2/3-d and CaCl2/3-d (14 and 10% respectively). It is concluded that at 12°C, the increase in GP would not justify the use of priming. At 25°C priming increased GP and reduced MGT. At -0.7 MPa priming increased germination, while at -1.0 MPa the increase is not agronomical considerable. At 12°C and -1.0 MPa the increase of germination is not agronomical important.

scholarly journals Seed Germination and Initial Growth of Pepper Seedlings is Influenced by Environment Temperature and Priming Method

2020 ◽  
Vol 8 (4) ◽  
pp. 540
Author(s):  
Glenda Sallaku ◽  
Gjok Vuksani ◽  
Astrit Balliu
Keyword(s):  
Polyethylene Glycol ◽  
Germination Percentage ◽  
Early Growth ◽  
Distilled Water ◽  
Initial Growth ◽  
Environment Temperature ◽  
Germination Speed ◽  
Mean Germination Time ◽  
Peg 8000 ◽  
Germination Parameters

The effects of different environment temperatures, priming treatments and priming durations on germination parameters and early growth of pepper seedlings were investigated. Seeds were hydro primed in distilled water, or osmo primed in two different water potentials (-0.15 and -0.5 MPa) of polyethylene glycol (PEG) 8000. Both, hydro primed and osmo primed seeds were subject of two different imbibitions periods, 48 and 72 hrs. In addition to primed seeds, an equal sample of non primed seeds was included in the experiment as a control variant. Following priming, the seeds were subjected to germination tests at two different environment temperatures, respectively 18 0C and 24 0C. In general, there was no significant effect of hydro priming on germination parameters or early growth of pepper seedlings. On the contrary, significantly higher germination percentage and germination speed and significantly shorter mean germination time than the non primed seeds were obtained due to seed osmo priming by PEG. Similarly, an enhanced seedling growth, especially under suboptimum temperature conditions was evidenced in PEG primed seedlings. Apart from germination speed, the increase of PEG concentration from -0.15 to -0.5 MPa had no effect to germination parameters of pepper seeds. The extent of the imbibitions period over 48 hrs did also have no benefits on germination parameters.

scholarly journals In vitro screening of gamma irradiated rice variety MR263 for drought tolerance using polyethylene glycol

Food Research ◽  
2020 ◽  
Vol 4 (S5) ◽  
pp. 11-20
Author(s):  
I. Tatar ◽  
A. Shamsiah ◽  
H.A. Rahim
Keyword(s):  
Polyethylene Glycol ◽  
Rice Variety ◽  
Agricultural Practices ◽  
Dry Weight ◽  
Weather Conditions ◽  
Germination Percentage ◽  
Gamma Irradiated ◽  
Polyethylene Glycol 6000 ◽  
Land Space

Fluctuating weather conditions and climate change are affecting agricultural production worldwide including rice yield. Combined with the increasing population and decreasing land space for agricultural practices, biotechnological solutions are needed to improve the livelihood of farmers and the country’s food security. Rice seeds of cv. MR263 were irradiated with gamma rays at doses 300, 350, and 400 Gy to evaluate their response to ionizing radiation on their growth. The irradiated seeds were germinated on MS media, and the seedlings were grown in media with polyethylene glycol 6000. It was found that most of the germination percentage decreased gradually with increasing radiation dosage. Shoot lengths reduced with radiation dose, per treatment. The root length was highest at dose 350 Gy (9.5 cm) compared to the other irradiated treatments. Fresh and dry weights generally decreased with increasing dosage and water stress levels except for dose 350 Gy. The highest fresh (354 mg) and dry weight (56.7 mg) were observed in seedlings irradiated with a dose of 350 Gy treated with 0% PEG and 10% PEG respectively. The content of proline and carbohydrates increased as the radiation doses and PEG concentrations increased where the highest proline (11.8 µM/g) was observed in 350 Gy + 20% PEG. While the highest carbohydrate content (9.3 mg/g) was observed in 400 Gy+ 20% PEG. It was concluded that seedlings radiated with 350 Gy showed promising results indicating tolerance to drought stress, due to their improved morphological and biochemical traits.

scholarly journals 836 PB 352 EFFECTS OF PRIMING ON MUSKMELON SEED STORABILITY

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 553a-553
Author(s):  
Melkizedek O. Oluoch ◽  
Gregory E. Welbaum
Keyword(s):  
Polyethylene Glycol ◽  
Moisture Content ◽  
Water Potential ◽  
Cucumis Melo ◽  
Seed Storage ◽  
Germination Percentage ◽  
Saturated Soils ◽  
Field Soils ◽  
Controlled Deterioration ◽  
Seed Storability

Priming (controlled hydration followed by drying) has been shown to decrease seed storage life in some species The germinablity of primed (0.3 M KNO3, 6 d, 25°C) and unprimed muskmelon (Cucumis melo L., cv. PMR 45) seeds were compared after storage for 9 yrs at less than 20°C and 6% moisture content (MC) (dwt basis). Germination performance was compared at 30°C in water and polyethylene glycol solutions of -0.2, -0.4, -0.6, -0.8, and -1.0 MPa water potential or in water at 15, 20, and 25°C. Seeds were also germinated in field soils at 17, 19, and 21% (dwt. basis) MC in a greenhouse, Some seeds were subjected to controlled deterioration at 20% MC and 45°C for 72 hrs prior to testing. The germination percentage and rate of stored, primed seeds at 30°C and all water potentials was less than stored, unprimed seeds. At 30°C, stored, unprimed seeds germinated more rapidly and to higher percentages at -0.2 MPa than in water, while germination percentages and rates of stored, primed seeds were essentially the same. At 15, 24, and 25°C, stored, primed seeds outperformed unprimed seeds in all germination tests. In saturated soils at 21% MC, there was no germination of either stored, primed or unprimed seed. At 17% soil MC, stored, primed seeds germinated 73% compared to only 56% for unprimed seeds. The enhancement due to priming was retained after 9 yrs of storage at germination temperatures <30°C. At higher temperatures, the germination of unprimed seeds was superior to primed.

scholarly journals Seed Priming Effect on Symbiotic Germination and Seedling Development of Orchis palustris Jacq.

HortScience ◽  
2004 ◽  
Vol 39 (7) ◽  
pp. 1700-1701 ◽  
Author(s):  
Ahmet Esitken ◽  
Sezai Ercisli ◽  
Cafer Eken ◽  
David Tay
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Priming Effect ◽  
Mycorrhizal Fungus ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seedling Development ◽  
Percentage Germination ◽  
Oatmeal Agar ◽  
Symbiotic Germination

Seeds of Orchis palustris Jacq. were primed for 1- to 5-day in polyethylene glycol (PEG-6000) solutions at -0.5, -1.0 or -1.5 MPa. The seeds were symbiotically germinated with BNR 8-3 mycorrhizal fungus on oatmeal agar at 22 °C. In general, priming hastened rapid germination. At -1.5 MPa water potential, the first to germinate was eight days compared to 18 days for the control. Percentage germination increased as priming water potential decreased, and the percentage germination was 55%, 58%, and 65%, at -0.5, -1.0, and -1.5 MPa, respectively, versus 43% for the nonprimed control. Priming duration from 1 to 5 days had little effect on germination performance. The best germination percentage (68%) was obtained from 1 day at -1.5 MPa treatment.

scholarly journals Influence of priming on Eucalyptus spp seeds' tolerance to salt stress

2016 ◽  
Vol 38 (4) ◽  
pp. 329-334 ◽  
Author(s):  
Anderson Cleiton José ◽  
Natália Cristina Nogueira Silva ◽  
José Marcio Rocha Faria ◽  
Wilson Vicente Souza Pereira
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Water Potential ◽  
Salt Solution ◽  
Germination Percentage ◽  
Eucalyptus Urophylla ◽  
Nacl Solution ◽  
Peg 6000 ◽  
Germination Speed ◽  
Osmotic Potentials

Abstract: The objective of this experiment was to evaluate the effect of priming on the germination of Eucalyptus urophylla and of hybrid E. urophylla × E. grandis seeds under salt stress. Two osmotic potentials (-1.0 and -1.5 MPa) were tested, using PEG 6000 for 1 and 3 days. After priming, seeds were germinated under salt stress in a NaCl solution at 0.0 (control), -0.5, -0.75 and -1.0 MPa potentials, at 25 °C. Seed germination and germination speed index decreased as the water potential of the germination medium decreased. However, E. urophylla was more tolerant to salt stress; it showed a higher germination percentage under all tested potentials, when compared to the hybrid. The osmotic conditioning at -1.0 MPa for three days was more effective when E. urophylla x E. grandis was germinated in a salt solution at -1.0 MPa, indicating that this treatment was more effective in inducing tolerance to salt stress.

Secondary Dormancy, Growth-Regulator Effects, and Embryo Growth Potential in Curly Dock (Rumex crispus) Seeds

Weed Science ◽  
1983 ◽  
Vol 31 (2) ◽  
pp. 153-158 ◽  
Author(s):  
Cyrus Samimy ◽  
Anwar A. Khan
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Growth Potential ◽  
Embryonic Cells ◽  
Rumex Crispus ◽  
Dark Incubation ◽  
Secondary Dormancy ◽  
Effective Removal ◽  
Radicle Protrusion ◽  
Polyethylene Glycol 6000

Curly dock (Rumex crispus L.) seeds failed to germinate in the dark, but germinated readily in light at 15, 20, and 25C. A secondary dormancy was induced in these seeds by 6 days of dark or light incubation at 30C (thermodormancy), by 6 days of dark incubation at 15C, or by a prolonged soaking in −15.7 bar polyethylene glycol-6000 (PEG) solution at 15C in light. The secondary dormancy induced at 15C was partially removed by 2-chloroethylphosphonic acid (ethephon) in presence of light; however, when the dormancy was induced at 30C, an addition of gibberellin A4+7 (GA4+7) to ethephon was necessary for effective removal of the dormancy. The ability of growth regulators to remove the secondary dormancy induced by prolonged dark incubation at 15C decreased progressively in light as the duration of dark incubation increased. The water-potential studies indicate that the thermodormancy can be attributed, at least in part, to the inability of the embryonic cells to lower their water potential to a level sufficient to cause radicle protrusion.

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