scholarly journals Seed Priming Effect on Symbiotic Germination and Seedling Development of Orchis palustris Jacq.

HortScience ◽  
2004 ◽  
Vol 39 (7) ◽  
pp. 1700-1701 ◽  
Author(s):  
Ahmet Esitken ◽  
Sezai Ercisli ◽  
Cafer Eken ◽  
David Tay
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Priming Effect ◽  
Mycorrhizal Fungus ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seedling Development ◽  
Percentage Germination ◽  
Oatmeal Agar ◽  
Symbiotic Germination

Seeds of Orchis palustris Jacq. were primed for 1- to 5-day in polyethylene glycol (PEG-6000) solutions at -0.5, -1.0 or -1.5 MPa. The seeds were symbiotically germinated with BNR 8-3 mycorrhizal fungus on oatmeal agar at 22 °C. In general, priming hastened rapid germination. At -1.5 MPa water potential, the first to germinate was eight days compared to 18 days for the control. Percentage germination increased as priming water potential decreased, and the percentage germination was 55%, 58%, and 65%, at -0.5, -1.0, and -1.5 MPa, respectively, versus 43% for the nonprimed control. Priming duration from 1 to 5 days had little effect on germination performance. The best germination percentage (68%) was obtained from 1 day at -1.5 MPa treatment.

scholarly journals 837 PB 355 A COMPARISON OF BROCCOLI SEED PRIMING TREATMENTS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 553b-553
Author(s):  
Lewis W. Jett ◽  
Gregory E. Welbaum
Keyword(s):  
Polyethylene Glycol ◽  
Calcium Silicate ◽  
Priming Effect ◽  
Field Experiments ◽  
Solid Phase ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Germination Percentage ◽  
The Mean ◽  
Mean Time

Various osmotic solutions and solid carriers have been used to prime (controlled hydration followed by drying) seeds. Broccoli (Brassica oleracea L., var. italica, cvs. Brigadier and Earlidawn) seeds were primed in solutions of polyethylene glycol, potassium nitrate (KNO3), and mannitol or calcium silicate (Micro-ccl E) and vermiculite to determine which treatment provided the greatest enhancement of germination. Germination percentage and rate as well as head yield were determined in a series of laboratory, glasshouse, and field experiments over a three year period. Water potentials (Y) ranged from -0.8 to -2.6 MPa at 20°C for both osmotic solutions and carriers. The duration of the priming treatments were 3, 7, or 10 days. All priming treatments, except KNO3 and mannitol, consistently reduced the mean time to germination in the laboratory, reduced the mean time to field emergence, increased final stands, but did not affect the mean time to harvest. However, solid phase was more effective than osmotic priming at each Ψ tested. The best solid priming mixture was 1.0:0.8:1.8 (seed:carrier:water) for 7 days at 20°C using Micro-cel E. This treatment was measured to have a Y = -1.2 MPa, and other types of priming at the same Y were less effective. The Y threshold below which no priming effect was obtained was Ψ= -2.4 MPa. Priming response was closely associated with, but not entirely dependent on, the Ψ of the treatment.

scholarly journals Osmotic Seed Priming of Rudbeckia fulgida Improves Germination and Expands Germination Range

HortScience ◽  
1994 ◽  
Vol 29 (8) ◽  
pp. 868-870 ◽  
Author(s):  
Amy M. Fay ◽  
Mark A. Bennett ◽  
Steven M. Still
Keyword(s):  
Polyethylene Glycol ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
The Mean ◽  
Mean Time

Low-vigor seeds of black-eyed Susan (Rudbeckia fulgida Ait.) primed in aerated -1.3 MPa KNO3 for 7 days at 30C in darkness had double the total germination percentage at 30C and one-half the mean time of germination as nonprimed seeds. Priming the seeds in polyethylene glycol rather than KNO3 generally resulted in lower total germination percentage and longer mean time of germination. Osmotic priming increased total germination percentage and germination rate of seeds germinated at 21.9 to 32.2C, but the priming benefit on total germination percentage was greater at ≤27.6C. Total germination percentage of primed and nonprimed seeds was highest at 28.8 to 32.2C.

scholarly journals Germination and Emergence of Parsley in Response to Osmotic or Matric Seed Priming and Treatment with Gibberellin

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 907-909 ◽  
Author(s):  
Wallace G. Pill ◽  
Elizabeth A. Kilian
Keyword(s):  
Polyethylene Glycol ◽  
Gibberellic Acid ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Petroselinum Crispum ◽  
Hypocotyl Length ◽  
Shoot Dry Weight ◽  
Emergence Percentage

`Moss Curled' seeds of parsley (Petroselinum crispum L.) were primed osmotically in polyethylene glycol or matrically in fine, exfoliated vermiculite at –0.5 MPa for 4 or 7 days at 20 or 30 °C with 0 or 1 mm GA3. All priming treatments stimulated and hastened germination. Matric priming resulted in greater germination (89%) than osmotic priming (83%) when seeds were primed for 7 days at 30 °C, but priming agent had no effect on germination percentage following priming at 20 °C or for 4 days. In seeds primed for 4 days at 20 or 30 °C, matric priming hastened germination more than did osmotic priming. Germination was generally less synchronous with matric than with osmotic priming. Increasing priming time from 4 to 7 days increased the rate of germination, but increased germination synchrony only when seeds were primed at 20 °C. Inclusion of 1 mm GA3 during priming had little or no effect on germination. All matric priming treatments (other than 4-day priming) were repeated to assess seedling emergence in a greenhouse (25°C day/22 °C night). Priming increased the percentage, rate and synchrony of emergence, and increased hypocotyl length at 3 weeks after planting. Priming at 30 °C with 1 mm GA3 resulted in the greatest emergence percentage, hypocotyl length, and shoot dry weight. We conclude that matric priming is a satisfactory alternative to osmotic priming of parsley seeds. Chemical name used: gibberellic acid (GA3).

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals 836 PB 352 EFFECTS OF PRIMING ON MUSKMELON SEED STORABILITY

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 553a-553
Author(s):  
Melkizedek O. Oluoch ◽  
Gregory E. Welbaum
Keyword(s):  
Polyethylene Glycol ◽  
Moisture Content ◽  
Water Potential ◽  
Cucumis Melo ◽  
Seed Storage ◽  
Germination Percentage ◽  
Saturated Soils ◽  
Field Soils ◽  
Controlled Deterioration ◽  
Seed Storability

Priming (controlled hydration followed by drying) has been shown to decrease seed storage life in some species The germinablity of primed (0.3 M KNO3, 6 d, 25°C) and unprimed muskmelon (Cucumis melo L., cv. PMR 45) seeds were compared after storage for 9 yrs at less than 20°C and 6% moisture content (MC) (dwt basis). Germination performance was compared at 30°C in water and polyethylene glycol solutions of -0.2, -0.4, -0.6, -0.8, and -1.0 MPa water potential or in water at 15, 20, and 25°C. Seeds were also germinated in field soils at 17, 19, and 21% (dwt. basis) MC in a greenhouse, Some seeds were subjected to controlled deterioration at 20% MC and 45°C for 72 hrs prior to testing. The germination percentage and rate of stored, primed seeds at 30°C and all water potentials was less than stored, unprimed seeds. At 30°C, stored, unprimed seeds germinated more rapidly and to higher percentages at -0.2 MPa than in water, while germination percentages and rates of stored, primed seeds were essentially the same. At 15, 24, and 25°C, stored, primed seeds outperformed unprimed seeds in all germination tests. In saturated soils at 21% MC, there was no germination of either stored, primed or unprimed seed. At 17% soil MC, stored, primed seeds germinated 73% compared to only 56% for unprimed seeds. The enhancement due to priming was retained after 9 yrs of storage at germination temperatures <30°C. At higher temperatures, the germination of unprimed seeds was superior to primed.

scholarly journals Effect of osmopriming on the emergence of Maize (Zea mays L.) seedling

2015 ◽  
Vol 39 (3) ◽  
pp. 427-435 ◽  
Author(s):  
KU Ahammad ◽  
MM Rahman ◽  
M Ahmed
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Seedling Emergence ◽  
Research Work ◽  
Seed Priming ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Saturated Sand ◽  
Germination Time ◽  
Mean Germination Time

The research work was carried out at the Seed Laboratory of Agronomy Department of Bangladesh Agricultural University, Mymensingh, Bangladesh during the period from March to November in 2008 to find out the effect of different osmopriming techniques on seedling emergence of maize. Seventeen osmopriming techniques viz., T1 = 1% Na2SO4, T2 = 3% Na2SO4, T3 = 5% Na2SO4, T4 = 1% K2HPO4, T5 = 3% K2HPO4, T6 = 5% K2HPO4, T7 = 1% ZnSO4, T8 = 3% ZnSO4, T9 = 5% ZnSO4, T10 = 1% Ca(H2PO4)2, T11 = 3% Ca(H2PO4)2, T12 = 5% Ca(H2PO4)2, T13 = 1% H2O2, T14 = 3% H2O2, T15 = 5% H2O2, T16 = Hydropriming, T17 = Non-priming (control) were used as experimental variables. Different osmopriming methods on seedling emergence performance of maize was evaluated at two moisture levels viz., 30 and 60% moisture of saturated sand in the experiment. Germination percentage, germination index and mean germination time were influenced significantly by osmopriming methods. Seed priming with 3% ZnSO4 showed the highest seedling emergence which was followed by 1% H2O2 and 3% Ca (H2PO4)2. DOI: http://dx.doi.org/10.3329/bjar.v39i3.21986 Bangladesh J. Agril. Res. 39(3): 427-435, September 2014

scholarly journals Effects of melatonin on germination and amino acid content in different wheat varieties seeds under polyethylene glycol stress

2019 ◽  
Author(s):  
Dongxiao Li ◽  
Di Zhang ◽  
Hongguang Wang ◽  
Haoran Li ◽  
Shijia Song ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Germination Percentage ◽  
Germination Index ◽  
Percentage Germination ◽  
Wheat Seed ◽  
Wheat Varieties ◽  
Radicle Length

AbstractIn this research, the effects of melatonin on germination and amino acid content in wheat (Triticum aestivum L.) seeds under polyethylene glycol (PEG-6000) stress were investigated. The results revealed that 10 μM melatonin could aggravate the adverse effects of drought stress on germination percentage, germination index, and germination potential of two wheat varieties (JM22 and HG35). However, 300 μM melatonin could obviously alleviate the negative effect of water stress on germination and increase radicle length, radicle number and plumule length of germinated seeds. Principal component analysis showed that amino acid content apparently changed in germination and the variation amplitude was different due to melatonin concentrations and wheat varieties. Lys content in wheat seed under 300 μM melatonin plus PEG treatment increased compared with that under PEG. Meanwhile, Lys content was significantly and positively correlated with germination percentage, germination index, germination potential, radicle length, and plumule length, respectively. Our results suggested Lys play an important role in the regulation of melatonin on drought resistance of wheat seed during germination. This may be of value for saving water resources in North China Plain.

Effect of Moisture Stress on Germination and Radicle Development ofPinuseldarica Medw. and Pinusponderosa Laws.

1975 ◽  
Vol 5 (1) ◽  
pp. 80-83 ◽  
Author(s):  
Karim Djavanshir ◽  
C. P. P. Reid
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Marked Reduction ◽  
Seedling Survival ◽  
Moisture Stress ◽  
Percentage Germination ◽  
Radicle Growth ◽  
Peg 4000 ◽  
Effect Of Moisture ◽  
Germination Value

Polyethylene glycol (PEG-4000) was used to study the effect of substrate water potential, ranging from 0 to 20 negative bars, on the germination of Pinusponderosa and P. eldarica seeds over a 35 day period. A marked reduction in percentage germination and germination value (GV) occurred at −4 bars for P. ponderosa and −6 bars for P. eldarica and optimum germination occurred at −2 and −4 bars, respectively. The best radicle growth (elongation) occurred at −2 bars for both species, but was severely reduced at potentials −4 bars or less. Although germination occurred at potentials as low as −8 bars for P. ponderosa and −12 bars for P. eldarica, it was estimated that percentage seedling survival would be near zero at these moisture stresses because of a marked reduction in radicle growth.

scholarly journals Improvement of Germination Characteristics and Enhancement of Antioxidant Enzymes Activity of Safflower (Carthamus tinctorius L.) Aged Seeds by Used of Gibberellin

2015 ◽  
Vol 48 (3) ◽  
pp. 33-41
Author(s):  
M. Khajeh ◽  
S.A. Tabatabaei ◽  
O. Ansari ◽  
F. Sharif Zadeh
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Enzymes ◽  
Ascorbate Peroxidase ◽  
Seed Priming ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Characteristics ◽  
Normal Seedling

AbstractSeed priming is one of the methods that can be taken to counteract the adverse effects of abiotic stress, also Seed priming treatments have been used to reduce the damage of aging and invigorate their performance in many crops. Objective of this study was to evaluation the effect of gibberellin on germination characteristics and antioxidant enzymes of safflower seeds after aging. Experimental design was a factorial one with complete randomized design with three replications. The first factor was priming by gibberellin (0 and 50 ppm) and non-primed seeds (control) and the secondary factor was combinations of four levels of aging (0, 1, 3 and 5 days of aging, at 43°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics such as; germination percentage, germination index, normal seedling percentage, seedling length, vigor index, catalase and ascorbate peroxidase were attained from priming by gibberellins, under non aged conditions. Also, our results indicated that seed aging is related to decrease of enzymes activity and may contribute to low seed germination efficiency, also priming increases enzyme activity and increases enzyme activity with priming treatment may contribute to improve germination characteristics. The general decreases in enzyme activity in the seed lowers the respiratory capacity, which in turn lowers both the energy (ATP) and assimilates supply of the germinating seed.

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