scholarly journals 836 PB 352 EFFECTS OF PRIMING ON MUSKMELON SEED STORABILITY

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 553a-553
Author(s):  
Melkizedek O. Oluoch ◽  
Gregory E. Welbaum
Keyword(s):  
Polyethylene Glycol ◽  
Moisture Content ◽  
Water Potential ◽  
Cucumis Melo ◽  
Seed Storage ◽  
Germination Percentage ◽  
Saturated Soils ◽  
Field Soils ◽  
Controlled Deterioration ◽  
Seed Storability

Priming (controlled hydration followed by drying) has been shown to decrease seed storage life in some species The germinablity of primed (0.3 M KNO3, 6 d, 25°C) and unprimed muskmelon (Cucumis melo L., cv. PMR 45) seeds were compared after storage for 9 yrs at less than 20°C and 6% moisture content (MC) (dwt basis). Germination performance was compared at 30°C in water and polyethylene glycol solutions of -0.2, -0.4, -0.6, -0.8, and -1.0 MPa water potential or in water at 15, 20, and 25°C. Seeds were also germinated in field soils at 17, 19, and 21% (dwt. basis) MC in a greenhouse, Some seeds were subjected to controlled deterioration at 20% MC and 45°C for 72 hrs prior to testing. The germination percentage and rate of stored, primed seeds at 30°C and all water potentials was less than stored, unprimed seeds. At 30°C, stored, unprimed seeds germinated more rapidly and to higher percentages at -0.2 MPa than in water, while germination percentages and rates of stored, primed seeds were essentially the same. At 15, 24, and 25°C, stored, primed seeds outperformed unprimed seeds in all germination tests. In saturated soils at 21% MC, there was no germination of either stored, primed or unprimed seed. At 17% soil MC, stored, primed seeds germinated 73% compared to only 56% for unprimed seeds. The enhancement due to priming was retained after 9 yrs of storage at germination temperatures <30°C. At higher temperatures, the germination of unprimed seeds was superior to primed.

Reimposition of conditional dormancy during air-dry storage of prechilled Sitka spruce seeds

1998 ◽  
Vol 8 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Steve K. Jones ◽  
Peter G. Gosling ◽  
Richard H. Ellis
Keyword(s):  
Moisture Content ◽  
Water Potential ◽  
Seed Storage ◽  
Sitka Spruce ◽  
Picea Sitchensis ◽  
Seed Moisture Content ◽  
Moisture Contents ◽  
Dry Storage ◽  
Low Water Potential ◽  
Negative Exponential

AbstractPrechilling seeds of Sitka spruce (Picea sitchensis[Bong.] Carr.) at 4°C with 30% moisture content for 12–14 weeks (84–98 d) removed conditional dormancy (i.e. they were then able to germinate at 10°C). The non-dormant status was preserved after redrying to 6% moisture content. However, conditional dormancy was gradually reimposed during subsequent air-dry storage at 4°C and 6% seed moisture content in all five seed lots tested. Further investigations with one seed lot showed that reimposition was reversed by a second prechill treatment, but was reimposed again during subsequent air-dry storage. The trend of dormancy reimposition within seed lots over time was quantified by negative exponential relations between ability to germinate at 10°C and duration of air-dry storage. The progress of dormancy reimposition was influenced by seed storage moisture content and was most rapid at 4–10%. At higher moisture contents (15 and 20%) the rate of the reimposition of conditional dormancy was much reduced, while at moisture contents of 25 and 30% further loss in dormancy occurred. Thus it is clear that dormancy reimposition occurred during storage at low water potential rather than solely during desiccation from high to low water potential.

Short-term aerated hydration for the improvement of seed quality in Brassica oleracea L

1992 ◽  
Vol 2 (1) ◽  
pp. 41-49 ◽  
Author(s):  
J. M. Thornton ◽  
A. A. Powell
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Germination Rate ◽  
Germination Percentage ◽  
Short Term ◽  
Brussels Sprouts ◽  
High Germination ◽  
Controlled Deterioration ◽  
Improved Performance ◽  
Aged Seeds

AbstractSeeds of the Brussels sprouts cultivar Asmer Aries and the cauliflower cultivar Hipop were subjected to ageing at 20% moisture content and 45°C for 24 or 30 h, respectively; all seeds retained high germination after ageing. Aerated hydration of unaged and aged seeds of both cultivars for 4–8 h at a range of temperatures (10–30°C), followed by drying, resulted in improved performance, except that germination percentage and rate of cauliflower were lower at 10°C. Thus, all treated seeds showed greater germination rate and seedling root length than the control, which may have resulted from the advancement of the process of germination. The deleterious effect of aerated hydration at 10°C on cauliflower could be explained by damage due to rapid imbibition; seeds that had imbibed slowly to close to full imbibition (41% moisture content) before aerated hydration showed no decrease in germination. The improvement of aged seeds after aerated hydration was also revealed by higher germination after the controlled-deterioration test, which indicated less deterioration in treated seeds. Furthermore, the optimum improvements for all seeds were observed at 25°C and were greater when the water was aerated than non-aerated. These observations indicate the activation of metabolic repair processes during aerated hydration, leading to a reversal of the deterioration sustained during ageing.

scholarly journals Model Dinamik Vigor Daya Simpan Benih Kedelai pada Penyimpanan Terbuka

2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Initial Moisture Content ◽  
Seed Viability ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Input Model ◽  
Seed Storability

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>

CHROMOSOME DAMAGE INDUCED BY ARTIFICIAL SEED AGING IN BARLEY. I. GERMINABILITY AND FREQUENCY OF ABERRANT ANAPHASES AT FIRST MITOSIS

1981 ◽  
Vol 23 (2) ◽  
pp. 267-280 ◽  
Author(s):  
Minoru Murata ◽  
Eric E. Roos ◽  
Takumi Tsuchiya
Keyword(s):  
Moisture Content ◽  
Seed Storage ◽  
Germination Percentage ◽  
Mitotic Division ◽  
Root Tips ◽  
Hordeum Vulgare L ◽  
Seed Moisture Content ◽  
Seed Aging ◽  
Seed Moisture ◽  
Higher Temperature

In order to study the genetic changes which occur during seed storage, barley (Hordeum vulgare L. 'Himalaya') seeds were subjected to artificial aging using six combinations of temperature (21 °C, 32 °C, and 38 °C) and seed moisture content (12% and 18%). With increasing time in storage, germination of the seeds was delayed and reduced. Abnormal seedlings without roots also occurred with increased storage. Higher temperature and seed moisture content induced rapid loss of germinability. At the first mitotic division in the root tips, the frequency of aberrant anaphases and of roots with aberrations increased with increased storage time. The frequencies of aberrant anaphases and of roots with aberrations were also increased by higher temperature and seed moisture content. Frequencies of aberrant anaphases and of roots with aberrations were negatively correlated with germination percentage. This indicated that the frequency of chromosomal aberrations induced by seed aging might be estimated from the germination percentages.

scholarly journals Drying and physiological quality of palheteira seeds (Clitoria fairchildiana R.A. Howard)

2020 ◽  
Vol 9 (9) ◽  
pp. e858998157
Author(s):  
Érica Coutinho David ◽  
Bressa Karolina Dias Cardoso ◽  
Josiene Amanda dos Santos Viana ◽  
Eniel David Cruz
Keyword(s):  
Moisture Content ◽  
Seed Quality ◽  
Seed Storage ◽  
Germination Percentage ◽  
Hot Water ◽  
Degraded Land ◽  
Seed Moisture Content ◽  
Seed Moisture ◽  
Physiological Quality

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.

scholarly journals Seed Priming Effect on Symbiotic Germination and Seedling Development of Orchis palustris Jacq.

HortScience ◽  
2004 ◽  
Vol 39 (7) ◽  
pp. 1700-1701 ◽  
Author(s):  
Ahmet Esitken ◽  
Sezai Ercisli ◽  
Cafer Eken ◽  
David Tay
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Priming Effect ◽  
Mycorrhizal Fungus ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seedling Development ◽  
Percentage Germination ◽  
Oatmeal Agar ◽  
Symbiotic Germination

Seeds of Orchis palustris Jacq. were primed for 1- to 5-day in polyethylene glycol (PEG-6000) solutions at -0.5, -1.0 or -1.5 MPa. The seeds were symbiotically germinated with BNR 8-3 mycorrhizal fungus on oatmeal agar at 22 °C. In general, priming hastened rapid germination. At -1.5 MPa water potential, the first to germinate was eight days compared to 18 days for the control. Percentage germination increased as priming water potential decreased, and the percentage germination was 55%, 58%, and 65%, at -0.5, -1.0, and -1.5 MPa, respectively, versus 43% for the nonprimed control. Priming duration from 1 to 5 days had little effect on germination performance. The best germination percentage (68%) was obtained from 1 day at -1.5 MPa treatment.

scholarly journals Physalis peruviana seed storage

2016 ◽  
Vol 20 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Cíntia L. M. de Souza ◽  
Manuela O. de Souza ◽  
Ronaldo S. Oliveira ◽  
Claudineia R. Pelacani
Keyword(s):  
Moisture Content ◽  
Chloride Solution ◽  
Storage Period ◽  
Seed Storage ◽  
Germination Percentage ◽  
Physalis Peruviana ◽  
High Germination ◽  
Different Temperatures ◽  
Solanaceae Family ◽  
Kinetics Of

ABSTRACT Physalis peruviana belongs to Solanaceae family and has a high nutritional and nutraceutical potential. The production is intended for fruit consumption and the propagation is mainly by seeds. This study aimed to evaluate the influence of priming on the kinetics of germination of P. peruviana seeds stored at different temperatures. The seeds were stored at 5 and 25 °C in a chamber saturated with zinc chloride solution and in liquid nitrogen (-196 °C). Every 4 months, the seeds were removed from storage for evaluation of germination and moisture content in the laboratory and emergence and development of seedlings in greenhouse. During the last evaluation at 16 months, the seeds under the same conditions were subjected to salt stress. The moisture content varied during the storage period, but was always higher for seeds kept at -196 ºC. These seeds kept high germination percentage in water until 16 months, regardless of the tested temperature; however, in salt solution the germination percentage was significantly reduced.

scholarly journals Effects of ultra-dry storage on seed germination and seedling growth of Handeliondendron bodinieri

Silva Fennica ◽  
2021 ◽  
Vol 55 (3) ◽  
Author(s):  
Chuan Xie ◽  
Tianfeng Liu ◽  
Song Guo ◽  
Jian Peng ◽  
Zailiu Li
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Moisture Content ◽  
Seedling Growth ◽  
Germination Rate ◽  
Soluble Sugar ◽  
Seed Storage ◽  
Seed Vigor ◽  
Protein Accumulation ◽  
Dry Storage

(H. Lév.) Rehder is a rare, endangered, and therefore, protected tree species native to China. However, there are serious limitations to the effective protection of the species, including a low seed germination-rate and difficult storage due to a high seed oil-content. Here, we evaluated the feasibility of ultra-dry seed storage and its effects on seedling growth. We used the silica gel method to prepare ultra-dry seeds with different moisture contents to find an optimal moisture content range (2.54%–4.77%). Ultra-dry treatment improved storability of . Furthermore, seeds with a moisture content of 4.77% stored at room temperature, and seeds with a moisture content of 3.97% stored at 4 °C yielded the best results. Priming with an appropriate concentration of polyethylene glycol had a certain repairing effect on ultra-dry stored seeds and improved seed vigor, with a two-day priming treatment with 20% polyethylene glycol having the best effect. Finally, compared with sand storage at 4 °C, ultra-dry storage promoted seedling growth and root development; furthermore, it alleviated storage damage to seeds, promoted soluble sugar and soluble protein accumulation, and increased seedling nitrogen, phosphorus, and potassium uptake. Therefore, ultra-dry storage can be effectively used to preserve seeds. Specifically, low-temperature storage of ultra-dry seeds with a moisture content of 3.97% enhanced seed vigor, and seedling growth and development.Handeliodendron bodinieriH. bodinieri seedsH. bodinieriH. bodinieriH. bodinieri

Effect of osmotic stress on Myrtus communis germination

Biologia ◽  
2012 ◽  
Vol 67 (1) ◽  
Author(s):  
Hamrouni Lamia ◽  
Souayah Naoufel ◽  
Khouja Larbi ◽  
Rejeb Néjib
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Germination Percentage ◽  
Myrtus Communis ◽  
Intraspecific Variations ◽  
Germination Ability ◽  
Germination Speed ◽  
Petri Dishes ◽  
Genetic Pool ◽  
Polyethylene Glycol 6000

AbstractThe effects of water potential on germination were studied in four provenances of Myrtus from different bioclimatic zones in Tunisia. Water potential between 0 and −10 bars were obtained using polyethylene glycol 6000 (PEG-6000) solutions. Seeds were planted in Petri dishes full of sterile perlite, and kept for one month under 1500 lux light with 16h photoperiod and at 25°C. A low water potential induced a reduction in germination percentage and germination speed. Significant variations between the provenances were found even under control conditions, especially at the speed germination level. The provenances from humid regions were the most susceptible to water stress, and their germination percentage showed significant reductions at −8 bars. These differences in germination ability of provenances might be attributed to intraspecific variations resulting from the effects of natural selection and genetic pool background.

scholarly journals Viability and Vigor of Osmotically Primed Muskmelon Seeds After Nine Years of Storage

1996 ◽  
Vol 121 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Melkizedek O. Oluoch ◽  
Gregory E. Welbaum
Keyword(s):  
Cucumis Melo ◽  
Germination Rate ◽  
Seedling Emergence ◽  
Testing Machine ◽  
Seed Storage ◽  
Dry Weight ◽  
Universal Testing ◽  
Controlled Deterioration ◽  
Mean Time ◽  
Reduced Water

The viability and vigor of osmotically primed (0.3 m KNO3, 6 days, 25C) and nonprimed `PMR 45' muskmelon (Cucumis melo L.) seeds were compared after storage for 9 years at <20C and 6% moisture content (MC, dry weight basis). Viability was compared at 20, 25, and 30C at water potentials of 0, -0.2, -0.4, -0.6, and -0.8 MPa and in soil. Additionally, stored primed and nonprimed seeds were either primed, aged (15% MC and 45C) for up to 8 days, or aged for 72 hours and primed. The force required to puncture 5-mm-long, micropylar seed pieces was measured using an Instron universal testing machine. Less force was required to puncture primed seed pieces at 0, 5, 15, 20, and 25 hours of imbibition, demonstrating that osmotic priming weakens the perisperm envelope tissue that the radicle must penetrate for germination to occur. In an earlier report, germination rate and final germination percentages were higher for osmotically primed seeds both in laboratory tests and field emergence studies conducted immediately after priming. After 9 years in storage, nonprimed seeds germinated to higher percentages in water at 30C and reduced water potential at all temperatures, while primed seeds germinated to higher percentages in water at 20 and 25C and exhibited a higher percentage of seedling emergence at a soil MC of 17%. Priming durations of ≤5 days had no effect on the viability, while longer durations decreased the viability of stored primed and nonprimed seeds. Priming generally decreased the log mean time to germination of stored nonprimed seeds but increased values for stored primed seeds. Controlled deterioration increased the log mean time to germination and decreased the viability of primed seeds faster than nonprimed seeds. Priming following controlled deterioration had no effect on nonprimed seeds and reduced the percent viability of primed seeds by 20%. Osmotic priming has a deleterious effect on the seed storage life of muskmelon seeds.

Sign in / Sign up

Export Citation Format

Share Document