Diversity and PAH growth abilities of bacterial strains isolated from a contaminated soil in Slovakia

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Andrea Puškárová ◽  
Mária Bučková ◽  
Katarína Chovanová ◽  
Jana Harichová ◽  
Edita Karelová ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Hydrocarbonoclastic Bacteria ◽  
Promising Strategy ◽  
Rrna Sequencing ◽  
Polycyclic Aromatic ◽  
Polluted Sites ◽  
Arthrobacter Oxydans ◽  
Environmental Burdens

AbstractDifferent abandoned industrial areas contaminated by polycyclic aromatic hydrocarbons (PAHs) are present in Slovakia. These environmental burdens are very dangerous to the health of human and environment. The bioremediation, based on the use of hydrocarbons degrading microorganisms, is a promising strategy to sanitize these polluted sites. The aim of this investigation was to assess the bacterial diversity of a PAHs-contaminated soil and to select the potential hydrocarbonoclastic bacteria which can be used for different bioremediation approaches. The bacterial strains were isolated on minimal medium agar supplemented with a mixture of PAHs. Seventy-three isolated strains were grouped by ribosomal interspacer analysis in 15 different clusters and representatives of each cluster were identified by 16S rRNA sequencing. The PAHs degradation abilities of all bacterial isolates were estimated by the 2,6-dichlorophenol indophenol assay and by their growth on minimal broth amended with a mixture of PAHs. Different kinds of strains, members of the genus Pseudomonas, Enterobacter, Bacillus, Arthrobacter, Acinetobacter and Sphingomonas, were isolated from the contaminated soil. Four isolates (Pseudomonas putida, Arthrobacter oxydans, Sphingomonas sp. and S. paucimobilis) showed promising PAHs-degrading abilities and therefore their possible employing in bioremediation strategies.

scholarly journals Bioaugmentation Treatment of a PAH-Polluted Soil in a Slurry Bioreactor

2020 ◽  
Vol 10 (8) ◽  
pp. 2837
Author(s):  
Rubén Forján ◽  
Iván Lores ◽  
Carlos Sierra ◽  
Diego Baragaño ◽  
José Luis R. Gallego ◽  
...  
Keyword(s):  
Microbial Populations ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Fingerprinting ◽  
Bacterial Strains ◽  
Fine Soil ◽  
Ring Compounds ◽  
Polycyclic Aromatic ◽  
Bioslurry Reactor ◽  
Polluted Sites ◽  
Real Scale

A bioslurry reactor was designed and used to treat loamy clay soil polluted with polycyclic aromatic hydrocarbons (PAHs). To this end, biostimulation alone, or combined with bioaugmentation with two bacterial strains (Rhodocccus erythropolis and Pseudomonas stuzeri) previously isolated from the polluted site, was applied. The PAH concentrations decreased notably after 15 days in all of the treatments. The concentrations of the two- and three-ring compounds fell by >80%, and, remarkably, the four- to six-ring PAHs also showed a marked decrease (>70%). These results thus indicate the capacity of bioslurry treatments to improve, notably, the degradation yields obtained in a previous real-scale remediation carried out using biopiles. In this sense, the remarkable results for recalcitrant PAHs can be attributed to the increase pollutants’ bioavailability achieves in the slurry bioreactors. Regarding bioaugmentation, although treatment with R. erythropolis led to a somewhat greater reduction of lighter PAHs at 15 days, the most time-effective treatment was achieved using P. stutzeri, which led to an 84% depletion of total PAHs in only three days. The effects of microbial degradation of other organic compounds were also monitored by means of combined qualitative and quantitative gas chromatography mass spectrometry (GC–MS) tools, as was the evolution of microbial populations, which was analyzed by culture and molecular fingerprinting experiments. On the basis of our findings, bioslurry technology emerges as a rapid and operative option for the remediation of polluted sites, especially for fine soil fractions with a high load of recalcitrant pollutants.

scholarly journals Screening and optimization of arsenic degrading bacteria and their potential role in heavy metal bioremediation

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Mehwish Iqtedar ◽  
Farah Aftab ◽  
Rabab Asim ◽  
Roheena Abdullah ◽  
Afshan Kaleem ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Arsenic Concentration ◽  
Cost Effective ◽  
Maximum Growth ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
High Concentration ◽  
Arsenic Tolerance ◽  
Degrading Bacteria ◽  
Soil And Water

Industrialization has added extremely toxic metalloid arsenic into the environment which at high concentration severely threatens the biota. Naturally, some microbes possess the ability to bio-accumulate metals and also to transform arsenite (As III) a toxic form to a non-toxic arsenate As V. The present study aimed to isolate arsenic resistant bacterias from the arsenic contaminated soil and water. Among eleven bacterial isolates, three FAs 1, 4 and 9 exhibited tolerance against sodium arsenite at 100mM concentration by achieving growth of 7.48×109,1.57×109 and 2.23×109 C.F.U./ml, respectively. Optimization at different conditions such as temperature, pH and arsenic concentration revealed high arsenic tolerance from isolate FAs 4 (5.33×108) at 37°C and FAs 1 (4.43×108 C.F.U./ml) at pH 7. Arsenic resistance at optimum conditions for the bacterial strains FAs 1, FAs 4 and FAs 9 showed maximum growth at 80mM concentration of arsenite. These bacterial isolates did not show redox ability to oxidize arsenite As III to arsenate As V. However bacterial isolates FAs 1, FAs 4 and FAs 9 were able to accumulate arsenic 39.16, 148 and 125 µg/L on the 4th, 3rd and 5th day of incubation, respectively. The isolates FAs 1, FAs 4 and FAs 9 were identified as Gram negative non endospore forming rods. In future, these novel isolates possess a great potential in biotechnology field, as bioremediation of arsenic contaminated soil and water can be done by employing arsenic accumulating bacteria which is an eco-friendly and cost effective method.

THE EFFECT OF BACTERIAL STRAINS ON THE ENZYMATIC ACTIVITY OF SOILS IN IMPACT ZONE OF ATAMAN LAKE

2020 ◽  
Author(s):  
V.V. Zinchenko ◽  
◽  
E.S Fedorenko ◽  
A.V Gorovtsov ◽  
T.M Minkina ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Dehydrogenase Activity ◽  
Contaminated Soil ◽  
Model Experiment ◽  
Impact Zone ◽  
Bacterial Strains ◽  
The Impact

As a result of the model experiment, an increase in the enzymatic activity of meadow chernozem of the impact zone of Ataman Lake with the introduction of a strains mixture of metal-resistant microorganisms into the soil was established. The experiment has shown that the application of bacterial strains increases the dehydrogenase activity of contaminated soil by 51.8% compared to the variant without remediation

STUDIES ON SOME BACTERIAL DISEASES IN OREOCHROMIS NILOTICUS WITH SPECIAL REFERENCES TO TREATMENT

2018 ◽  
pp. 39-47
Author(s):  
Viola Zaki ◽  
Ahmed EL-gamal ◽  
Yasmin Reyad
Keyword(s):  
Oreochromis Niloticus ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Histopathological Examination ◽  
Klebsiella Oxytoca ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Tissue Samples ◽  
Hydropic Degeneration ◽  
Total Prevalence

he present research carried out to study the common bacterial infections in Oreochromis niloticus (Nile tilapia) in Manzala area at Dakahlia governorate and possible antimicrobial agents used for treatment. A total number of 400 fish were randomly collected from Manzala private farms at Dakahlia governorate and subjected to the clinical, bacteriological and histopathological examination. The highest prevalence of bacterial isolates during the whole period of examination of naturally infected O.niloticus was recorded for A.hydrophila (22.66%), followed by V.alginolyticus (19.01%), V.parahemolyticus (13.80%), Streptococcus spp. (12.24%), A.caviae (11.72%), V.cholera (10.16%), A.salmonicida (7.55%), while the lowest prevalence was recorded for Klebsiella oxytoca (2.86%). The seasonal highest total prevalence of bacterial isolates from examined naturally infected O. niloticus was recorded in spring (30.21%), followed by autumn (28.39%), then summer (22.40%) and the lowest prevalence was recorded in winter (19.01%). Histopathological findings of the tissue samples which collected from different organs of naturally infected O.niloticus revealed that spleen show marked hemosiderosis and sever hemorrhage, gills showsever congestion of lamellar capillaries with marked aneurysm, necrosis and hemorrhage of lamellar epithelium and liver show sever hydropic degeneration and necrosis of hepatocytes, Ciprofloxacin was the most effective antibiotic against all isolated bacterial strains

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

Bacterial Strains Isolated from Heavy Metals Contaminated Soil and Wastewater with Potential to Oxidize Arsenite

2021 ◽  
Vol 8 (1) ◽  
pp. 333-347
Author(s):  
Shahid Sher ◽  
Abdul Ghani ◽  
Sikandar Sultan ◽  
Abdul Rehman
Keyword(s):  
Heavy Metals ◽  
Contaminated Soil ◽  
Bacterial Strains

scholarly journals Ecological Response in the Integrated Process of Biostimulation and Bioaugmentation of Diesel-Contaminated Soil

2021 ◽  
Vol 11 (14) ◽  
pp. 6305
Author(s):  
Xiaosen Li ◽  
Yakui Chen ◽  
Xianyuan Du ◽  
Jin Zheng ◽  
Diannan Lu ◽  
...  
Keyword(s):  
Nitrogen Cycle ◽  
Contaminated Soil ◽  
Quantitative Polymerase Chain Reaction ◽  
Chemical Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Functional Genes ◽  
Alkane Degradation ◽  
Degrading Bacteria ◽  
Polycyclic Aromatic ◽  
Amine Compounds

The study applied microbial molecular biological techniques to show that 2.5% to 3.0% (w/w) of diesel in the soil reduced the types and number of bacteria in the soil and destroyed the microbial communities responsible for the nitrogen cycle. In the meantime, the alkane degradation gene alkB and polycyclic aromatic hydrocarbons (PAHs) degradation gene nah evolved in the contaminated soil. We evaluated four different remediation procedures, in which the biostimulation-bioaugmentation joint process reached the highest degradation rate of diesel, 59.6 ± 0.25% in 27 days. Miseq sequencing and quantitative polymerase chain reaction (qPCR) showed that compared with uncontaminated soil, repaired soil provides abundant functional genes related to soil nitrogen cycle, and the most significant lifting effect on diesel degrading bacteria γ-proteobacteria. Quantitative analysis of degrading functional genes shows that degrading bacteria can be colonized in the soil. Gas chromatography-mass spectrometry (GC-MS) results show that the components remaining in the soil after diesel degradation are alcohol, lipids and a small amount of fatty amine compounds, which have very low toxicity to plants. In an on-site remediation experiment, the diesel content decreased from 2.7% ± 0.3 to 1.12% ± 0.1 after one month of treatment. The soil physical and chemical properties returned to normal levels, confirming the practicability of the biosimulation-bioaugmentation jointed remediation process.

scholarly journals Remediation of Anthracene-Contaminated Soil with Sophorolipids-SDBS-Na2SiO3 and Treatment of Eluting Wastewater

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2188
Author(s):  
Wei Li ◽  
Xiaofeng Wang ◽  
Lixiang Shi ◽  
Xianyuan Du ◽  
Zhansheng Wang
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Organic Pollutants ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soil ◽  
Ring Opening ◽  
Single Factor ◽  
Elution Time ◽  
Factor B ◽  
Ring Opening Reaction ◽  
Polycyclic Aromatic

The soil pollution of polycyclic aromatic hydrocarbons (PAHs) is serious in China, which not only affects the living and growing environment of plants and animals but also has a great impact on people’s health. The use of hydrophobic organic compounds to make use of surfactant ectopic elution processing is more convenient and cheaper as a repair scheme and can effectively wash out the polycyclic aromatic hydrocarbons in the soil. Therefore, we mixed sophorolipids:sodium dodecylbenzene sulfonate (SDBS):Na2SiO3 according to the mass ratio of 1:15:150. We explored the influencing factors of high and low concentrations of PAH-contaminated soil using a single factor test and four factors at a two-level factorial design. Then, the elution wastewater was treated by ultrasonic oxidation technology and the alkali-activated sodium persulfate technology. The results showed that: (1) In the single factor test, when the elution time is 8 h, the concentration of the compounded surfactant is 1200 mg/L, the particle size is 60 mesh, the concentration of NaCl is 100 mmol/L, and the concentration of KCl is 50 mmol/L, and the effect of the PAH-contaminated soil eluted by the composite surfactant is the best. Externally added NaCl and KCl salt ions have a more obvious promotion effect on the polycyclic aromatic hydrocarbon-contaminated soil; (2) in the interaction experiment, single factor B (elution time) and D (NaCl concentration) have a significant main effect. There is also a certain interaction between factor A (concentration agent concentration) and factor D, factor B, and factor C (KCl concentration); (3) the treatment of anthracene in the eluate by ultrasonic completely mineralizes the organic pollutants by the thermal and chemical effects produced by the ultrasonic cavitation phenomenon, so that the organic pollutants in the eluate are oxidized and degraded into simple environmentally friendly small molecular substances. When the optimal ultrasonic time is 60 min and the ratio of oxidant to activator is 1:2, the removal rate of contaminants in the eluent can reach 63.7%. At the same time, the turbidity of the eluent is significantly lower than that of the liquid after centrifugal separation, indicating that oxidants can not only remove the pollutants in elution water but also remove the residual soil particulate matter; and (4) by comparing the infrared spectrum of the eluted waste liquid before and after oxidation, it can be seen that during the oxidation process, the inner part of eluent waste liquid underwent a ring-opening reaction, and the ring-opening reaction also occurred in the part of the cyclic ester group of the surfactant, which changed from a ring to non-ring.

Microbial Properties and Degradation of Polycyclic Aromatic Hydrocarbons in the Contaminated Farmland Soil with Bioremediation

2014 ◽  
Vol 665 ◽  
pp. 534-537
Author(s):  
Hong Wang ◽  
Hai Bo Li ◽  
Xin Wang ◽  
Ji Fu Ma
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Contaminated Soil ◽  
Significant Positive Correlation ◽  
Removal Rate ◽  
Microbial Properties ◽  
Total Pahs ◽  
Farmland Soil ◽  
Polycyclic Aromatic ◽  
Microbial Agent

Degradation of polycyclic aromatic hydrocarbons (PAHs) and microbial quantity were investigated in aged PAHs-contaminated soil in a pot experiment with regrass and alfalfa. After 60 days germination the concentration of total PAHs in soil decreased by 37.57% and 38.41% with the treatment of ryegrass-microbe agent and alfalfa-microbe agent. The processes of ryegrass and alfalfa were 18.72% and 19.34%. The root system promoted the quantity of microbe and the microbial agent was benefit for the PAHs degrading microbe. And there was significant positive correlation between the number of PAHs degrading microorganisms and the removal rate of PAHs in the soil.

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