ISOLASI SENYAWA TURUNAN NAPTOKUINON DARI KULIT BATANG FALOAK (Sterculia quadrifida R.BR) DAN UJI AKTIVITAS ANTIKANKER PADA SEL KANKER PAYUDARA JENIS T47D

ABSTRAK: Faloak (Sterculia quadrifida R.Br) digunakan secara empiris oleh penduduk Nusa Tenggara Timur untuk mengobati hepatitis, tifus, maag, dan pemulih stamina. Informasi senyawa aktif yang terkandung didalam kulit faloak secara spesifik belum dipublikasi. Penelitian ini bertujuan untuk mengetahui senyawa aktif yang terdapat didalam kulit faloak sebagai antikanker. Ekstraksi menggunakan metode maserasi, isolasi menggunakan metode isolasi bertingkat, elusidasi menggunakan penggabungan informasi dari spektra IR, 1D-NMR, 2D-NMR dan LC-MS, dan uji aktivitas antikanker pada sel kanker payudara T47D menggunakan metode MTT. Hasil isolasi diperoleh isolat turunan senyawa naptokuinon yaitu 2,3-dihydro-6-hydroxy-2-methylenenaphtho[1,2-b]furan-4,5-dione yang aktif sebagai antikanker dengan nilai IC50 pada sel kanker payudara sebesar 9,88 µg/mL dan dengan nilai selektivitas indeks sebesar 30,23. ABSTRACT: Faloak (Sterculia quadrifida R.Br) is used empirically by residents of East Nusa Tenggara to treat hepatitis, typhoid, ulcers, and stamina restorers. The information of the active compounds contained in the faloak skin is not specifically published. This study aims to determine the active compounds contained in the bark of faloak as anticancer. The extraction was conducted with maceration method followed by a multilevel isolation method. The elucidation was carried out using information of IR spectra, 1D-NMR, 2D-NMR and LC-MS. The anticancer activity test on T47D breast cancer cells was also conducted using MTT method. Based on the results obtained, the active compound is naphthoquinone derivative compound which is 2,3-dihydro-6-hydroxy-2-methylenenaphtho [1,2-b] furan-4,5-dione that has anticancer activity on breast cancer cell (T47D) with IC50 value of 9.88 µg/mL and index selectivity value of 30.23.

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Anticancer Activity against Breast Cancer Cells T47D and Identification of Its Compound from Extracts and Fractions of Leaves Bamboo Grass (Lophaterum gracile B.)

ALCHEMY ◽

10.18860/al.v4i1.3138 ◽

2015 ◽

Vol 4 (1) ◽

Cited By ~ 1

Author(s):

Alfi Istiqomah ◽

Roihatul Muti'ah ◽

Elok Kamilah Hayati

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Active Compound ◽

Breast Cancer Cells ◽

Hexane Fraction ◽

Active Compounds ◽

Ethanol Extraction ◽

Mtt Method ◽

Weed Plants

Bamboo grass (Lophatherum gracile B.) is one of weed plants which frequently used as a medicine because it provides active compounds, for instance steroids and tannins. This research was conducted to determine the anticancer activity of extracts and fractions of leaves bamboo grass in inhibiting the growth of breast cancer cells T47D, and to determine the class of the active compounds contained in extracts and fractions which have the lowest IC<sub>50</sub> value from phytochemical test and identification with UV-Vis instruments and FTIR. The extraction of active compound leaves of bamboo grass is done by maceration method using ethanol 80%, hydrolysis and partition with n-hexane and chloroform. Four of the extracts is phytochemical tested and anticancer activity tested towards breast cancer cells T47D with MTT method. The best results of the test activities are performed by the separation of classes of active compounds KLTP and are identified using UV-Vis spectrophotometer and FTIR. The value of IC<sub>50</sub> from 80% ethanol extraction is 321,389, from hydrolysis extraction is 481.984, from the fraction of chloroform is 177.852, and from n-hexane fraction is 300.681 µg / mL. The lowest IC50 which have the best cytotoxic is the fraction of chloroform and n-hexane. The results of phytochemical test fractions of chloroform and n-hexane fraction indicates the tannin and steroids. The identification result by using UV-Vis and FTIR shows that the fraction of chloroform and n-hexane fraction is containing tannin, which isproven by maximum wavelength at 331.6 nm and 331.6 nm allegations of -OH, CH, C=C. Moreover, the existence of steroid compounds in fraction chloroform and n-hexane is shown by the absorption at 279.1 nm and 278.0 nm of wavelength with allegations of -OH, CH, C=C, C-O.

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Cytotoxic Activity of BornUSU I towards T47D Breast Cancer Cells

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.511 ◽

2019 ◽

Vol 7 (22) ◽

pp. 3816-3818

Author(s):

Nadiya Anandita Nasution ◽

Urip Harahap ◽

Ginda Haro ◽

Hari Purnomo ◽

Denny Satria

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Incubation Period ◽

Breast Cancer Cells ◽

In Vitro Study ◽

Positive Control ◽

Breast Cancer Treatment ◽

Mtt Method ◽

Ic50 Value

AIM: The aim of this study was to determine cytotoxic activity of BornUSU I or Boronhafagama I (1,5-bis(4-hydroxyphenyl)-3-oxa-1,5-diaza-2,4-diboropentane-2,4-diol) as a boron derivate compounds which are boron neutron captured theraphy (BNCT) candidates. METHODS: The T47D cells were treated by BornUSU I, and Tamoxifen as a positive control. The in vitro study was using MTT method with the incubation period for 24h and 48h. All data were determined using viability of cells equation for showing each IC50 value. RESULTS: The IC50 value of BornUSU I and Tamoxifen were 72.61 ± 0.82 µM and 10.62 ± 0.06 µM for 24 h incubation period, and for the 48 h incubation period were 44.63 ± 0.23 µM and 7.79 ± 0.05 µM. The 48 h incubation period results showed the lowest IC50 value. CONCLUSION: The results reveal that BornUSU I provide effective as anticancer, especially for breast cancer treatment.

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ANTICANCER ACTIVITY OF ANNONA MURICATA EXTRACT ON TRIPLE NEGATIVE BREAST CANCER CELLS AND ISOLATION/CHARCTERIZATION OF ACTIVE COMPOUNDS

The FASEB Journal ◽

10.1096/fasebj.2018.32.1_supplement.565.8 ◽

2018 ◽

Vol 32 (S1) ◽

Author(s):

Heba Alshaeri ◽

Moudi Alasmari ◽

Alejandro Pino‐Figueroa

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Triple Negative Breast Cancer ◽

Breast Cancer Cells ◽

Triple Negative ◽

Annona Muricata ◽

Active Compounds

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Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

Current Biochemistry ◽

10.29244/cb.5.3.11-20 ◽

2019 ◽

Vol 5 (3) ◽

pp. 11-20

Author(s):

Stefani Dhale Rale ◽

Hasim Hasim ◽

Syamsul Falah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Inhibition Activity ◽

Active Compounds ◽

Activity Inhibition ◽

Activity Test ◽

Ic50 Value ◽

Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

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Anticancer activity in 2-methoxy-4-((4-methoxy-phenilimino)-methyl)-phenol compound on T47D breast cancer cells

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/456/1/012014 ◽

2020 ◽

Vol 456 ◽

pp. 012014

Author(s):

L Sukria ◽

E K Hayati ◽

A Hanapi ◽

R Adawiyah ◽

R Ningsih

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Phenol Compound

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Anticancer activity of uncaria gambir roxb on T47D breast cancer cells

Journal of Physics Conference Series ◽

10.1088/1742-6596/1317/1/012106 ◽

2019 ◽

Vol 1317 ◽

pp. 012106

Author(s):

Siti Syarifah ◽

Tri Widyawati ◽

Dwi Rita Anggraini ◽

Arlinda Sari Wahyuni ◽

Mutiara Indah Sari

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Breast Cancer Cells

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Mechanistic Study on Thymoquinone Conjugated ZnO Nanoparticles Mediated Cytotoxicity and Anticancer Activity in Triple Negative Breast Cancer Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520621666210412104731 ◽

2021 ◽

Vol 21 ◽

Author(s):

Sampath K. Banupriya ◽

Krishnamoorthy Kavithaa ◽

Arumugam Poornima ◽

Sundaravadivelu Sumathi

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Triple Negative Breast Cancer ◽

Zno Nanoparticles ◽

Breast Cancer Cells ◽

Triple Negative ◽

Molecular Techniques ◽

Mechanistic Study ◽

Spectroscopic Techniques

Background: In the current era, development of molecular techniques involves nanotechniques and the synthesis of nanoparticles is considered as the preferred field in nanotechnology. Objective: The aim of the present work is to analyze the anticancer activity of the thymoquinone conjugated ZnO nanoparticles and to understand its mechanism of action in triple negative breast cancer cell line MDA-MB-231. Methods: Zinc Oxide (ZnO) nanoparticles have extensive applications and it was synthesized using a chemical precipitation method. Thymoquinone (TQ) is the major bioactive component of the seeds of Nigella sativa. Synthesized nanoparticles were characterized using various spectroscopic techniques. Thymoquinone coated nanoparticles were checked for its efficiency. The cytotoxicity of ZnO, TQ and TQ conjugated ZnO nanoparticles against MDA-MB-231. Colony forming and cell migration assay were performed to measure the proliferative competence of the breast cancer cells on exposure to nanoparticles. The mechanism of apoptosis was probed by assessing MMP, interplay between ER stress and ROS. Results: The results of the characterization techniques confirmed the particles synthesized were ZnO and TQ-ZnO nanoparticles. pH dependent release of the compound was observed. Anti-proliferative effect that impairs the formation of colony was found to be enhanced in cells exposed to combined treatment with the nanoconjugate. Conclusion: Hence, the TQ conjugated ZnO nanoparticles can act as an efficient carrier for drug delivery at the target site in TNBC cells.

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Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells

IET Nanobiotechnology ◽

10.1049/iet-nbt.2016.0222 ◽

2017 ◽

Vol 11 (8) ◽

pp. 965-972 ◽

Cited By ~ 58

Author(s):

Ramasamy Subbaiya ◽

Muthupandian Saravanan ◽

Andavar Raja Priya ◽

Konathala Ravi Shankar ◽

Masilamani Selvam ◽

...

Keyword(s):

Breast Cancer ◽

Silver Nanoparticles ◽

Anticancer Activity ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Biomimetic Synthesis ◽

Human Breast Cancer Cells ◽

Human Breast

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Mummy Induces Apoptosis Through Inhibiting of Epithelial-Mesenchymal Transition (EMT) in Human Breast Cancer Cells

Galen Medical Journal ◽

10.31661/gmj.v9i0.1812 ◽

2020 ◽

Vol 9 ◽

pp. 1812

Author(s):

Solmaz Rahmani Barouji ◽

Arman Shahabi ◽

Mohammadali Torbati ◽

Seyyed Mohammad Bagher Fazljou ◽

Ahmad Yari Khosroushahi

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Anticancer Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Epithelial Mesenchymal Transition ◽

Control Group ◽

Mrna Levels ◽

Mesenchymal Transition ◽

Mcf 7

Background: Mummy (Iranian pure shilajit) is a remedy with possessing anti-inflammatory, antioxidant and anticancer activities. This study aimed to examine mummy effects on epithelial-mesenchymal transition (EMT) and invasiveness of MCF-7 and MDA-MB-231 breast cancer (BC) cell lines with underlying its mechanism. Materials and Methods: The dose-dependent inhibitory effect of the mummy on cell proliferation in vitro was determined using the MTT assay. Flow cytometry and 4’,6-diamidino-2-phenylindole dihydrochloride staining were respectively used for quantitative and qualitative analysis of cellular apoptosis, and gene expression analysis was conducted using real-time PCR. Results: MDA-MB-231 showed more sensitivity than the MCF-7 cell line to the anticancer activity of mummy, while mummy did not exhibit significant cell cytotoxicity against human normal cells (MCF-10A). The gene expression profile demonstrated a significant decrease in TGF-β1, TGF-βR1, TWIST1, NOTCH1, CTNNB1, SRC along with an increase in E-cadherin mRNA levels in mummy treated cells compared to the untreated control group (P≤0.05). Conclusion: Mummy triggers inhibition of EMT and metastasis in breast cancer cells mainly through the downregulation of TGFβ1 activity, and more studies required to find its specific anticancer activity with details. [GMJ.2020;9:e1812]

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