scholarly journals PLASTY OF DURA MATER DEFECTS BY COLLAGEN IMPLANTS USING NON-SUTURE CONTACT DIRECT BONDING METHOD

2017 ◽  
Vol 176 (2) ◽  
pp. 70-76
Author(s):  
D. E. Alekseev ◽  
D. V. Svistov ◽  
D. E. Matsko ◽  
E. D. Alekseev
Keyword(s):  
Dura Mater ◽  
Collagen Matrix ◽  
Laboratory Animals ◽  
Control Group ◽  
Meaningful Difference ◽  
Collagen Matrices ◽  
Fourth Group ◽  
Bonding Method ◽  
Dural Defects

OBJECTIVE. The study investigated and compared the efficacy of plasty of dura mater using different collagen materials in the experiment. MATERIALS AND METHODS. The experimental study in vivo was carried out on 60 laboratory animals. Rabbits were divided into 4 groups after performing the craniotomy and dura mater opening. It depended on the material used for plasty of dural defects such as Duraform, Lyostypt and Russian prototype of collagen matrix for dural grafting. The fourth group was the control group. The presence and severity of meningo-cerebral adhesion, histological and immunological researches of duraplasty areas were performed after the experiment. RESULTS. The severity of meningo-cerebral adhesion was significantly higher in control group, but there wasn’t noted any meaningful difference in the groups with different collagen matrices. These groups had similar histological and immunohistochemical features. CONCLUSIONS. An application of collagen matrix improved the results of duraplasty and prevented formation of meningo-cerebral adhesions in the experiment. This data predict the high clinical efficacy of the method.

scholarly journals In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices.

1983 ◽  
Vol 97 (5) ◽  
pp. 1648-1652 ◽  
Author(s):  
R Montesano ◽  
L Orci ◽  
P Vassalli
Keyword(s):  
Endothelial Cells ◽  
Three Dimensional ◽  
Collagen Matrix ◽  
Collagen Gels ◽  
Collagen Matrices ◽  
Narrow Lumen ◽  
Capillary Endothelial Cells ◽  
Vitro Model

We have studied the behavior of cloned capillary endothelial cells grown inside a three dimensional collagen matrix. Cell monolayers established on the surface of collagen gels were covered with a second layer of collagen. This induced the monolayers of endothelial cells to reorganize into a network of branching and anastomosing capillary-like tubes. As seen by electron microscopy, the tubes were formed by at least two cells (in transverse sections) delimiting a narrow lumen. In addition, distinct basal lamina material was present between the abluminal face of the endothelial cells and the collagen matrix. These results showed that capillary endothelial cells have the capacity to form vessel-like structures with well-oriented cell polarity in vitro. They also suggest that an appropriate topological relationship of endothelial cells with collagen matrices, similar to that occurring in vivo, has an inducive role on the expression of this potential. This culture system provides a simple in vitro model for studying the factors involved in the formation of new blood vessels (angiogenesis).

scholarly journals Prospects for the use of a drug based on a complex of fatty acid esters in models of laboratory animals in vivo

2021 ◽  
Vol 37 ◽  
pp. 00175
Author(s):  
K.Yu. Smirnova ◽  
A.V. Bannikova ◽  
S.V. Kozlov ◽  
O.V. Romanova
Keyword(s):  
Fatty Acid ◽  
Guinea Pigs ◽  
Fatal Outcome ◽  
Fatty Acid Esters ◽  
Laboratory Animals ◽  
Control Group ◽  
Burn Wound ◽  
Complicated Course ◽  
Acid Esters

In this work we carried out studies of the first obtained micellar composition of fatty acid esters with allantoin on a water basis to determine its local irritant effect and therapeutic efficacy in the treatment of burns. It was found that there was a sequential change in the phases of the burn wound process in all groups. During the observation period, wound suppuration was not observed; there were 4 cases of a complicated course of the wound process and a fatal outcome in the control group. When conducting studies of local irritant effects, the assessment was carried out in points, in the experimental group it was on average 2 points as for the animals of the control group. The conducted studies allow us to conclude that the drug, when applied to the skin according to the interstate standard GOST ISO 10993-10—2011, has a weak degree of response to irritation in guinea pigs. The application of a permissive dose of the drug to sensitized guinea pigs does not cause a response from the skin, which indicates the absence of allergenic properties in this composition.

scholarly journals EXPERIMENTAL STUDY OF THE NEUROTOXIC EFFECTS OF PHOTODYNAMIC THERAPY ON THE SPINAL CORD

2019 ◽  
Vol 18 (3) ◽  
pp. 176-180
Author(s):  
HERTON RODRIGO TAVARES COSTA ◽  
ELAINE APARECIDA DEL BEL BELLUZ GUIMARÃES ◽  
ANTÔNIO CLAUDIO TEDESCO ◽  
FERNANDO LUCAS PRIMO ◽  
CÉLIA APARECIDA DA SILVA ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Dura Mater ◽  
Aluminum Chloride ◽  
Control Group ◽  
Functional Evaluation ◽  
Neurotoxic Effects ◽  
Pathological Evaluation ◽  
Male Wistar Rats

ABSTRACT Objective To evaluate the effects of photodynamic therapy (PDT) on the dura mater using the photosensitizers aluminum chloride phthalocyanine and methylene blue in in vivo assays. Methods Fifty-six male Wistar rats were divided into two groups; one submitted to PDT and the other submitted to the photosensitizers without their photoactivation (control). The photosensitizers were applied to the dura mater after laminectomy at the T10 level. The methods used for assessment were the Basso, Beattie and Bresnahan (BBB) functional evaluation scale and study of the dura mater by light microscopy. Results No changes in motor activity were observed in the animals submitted to PDT compared to control. Histological and pathological evaluation did not show any differences between the group exposed to activated photosensitizers and the control group with regard to the inflammatory process and tissue necrosis. Conclusion The joint use of PDT with the photosensitizing pharmaceuticals aluminum chloride phthalocyanine and methylene blue did not induce any clinical neurotoxic effects or histological changes in the dura mater of the animals studied. Level de evidence V; Expert Opnion.

Fibrin-Reinforced Collagen Matrix Enhances Tissue Regeneration and Angiogenesis

2005 ◽  
Vol 288-289 ◽  
pp. 257-260
Author(s):  
Dong Lim Seol ◽  
Won Hee Jang ◽  
Sung Jae Lee ◽  
Young Il Yang
Keyword(s):  
Tissue Regeneration ◽  
Nude Mice ◽  
Functional Integration ◽  
Collagen Matrix ◽  
Dermal Fibroblasts ◽  
Surrounding Tissue ◽  
Human Dermal Fibroblasts ◽  
Collagen Matrices

The goal of this study was to investigate effects of fibrin reinforcement of collagen sponges on fibroblasts-mediated contraction and in vivo tissue regeneration, especially angiogenesis. Human dermal fibroblasts (HDFs)-populated collagen sponges reinforced with or without fibrin were cultivated via the free-floating method in vitro. They were then evaluated using xenogeneic implantation into nude mice. The HDFs-populated collagen sponges reinforced with fibrin exhibited significantly decreased HDFs-mediated contraction in vitro (p<0.05). Microvascular and cellular densities of the collagen sponges were significantly higher with fibrin than without (p<0.01). Cell ingrowths, neovascularization, and deposition of ECM matrix were more evenly distributed in the fibrin-reinforced collagen matrices. The results demonstrated that fibrin reinforcement of porous collagen sponges can reduce cell-mediated contraction in vitro while enhancing functional integration with surrounding tissue in vivo.

scholarly journals Evaluation of in Vivo Response of Three Biphasic Scaffolds for Osteochondral Tissue Regeneration in a Sheep Model

2019 ◽  
Vol 6 (4) ◽  
pp. 90 ◽  
Author(s):  
Alberto M. Crovace ◽  
Alessia Di Giancamillo ◽  
Francesca Gervaso ◽  
Laura Mangiavini ◽  
Davide Zani ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Collagen Matrix ◽  
Control Group ◽  
The Novel ◽  
Sheep Model ◽  
Complete Restoration ◽  
Different Types ◽  
Ct Analysis ◽  
Reparative Process

Osteochondral defects are a common problem in both human medicine and veterinary practice although with important limits concerning the cartilaginous tissue regeneration. Interest in the subchondral bone has grown, as it is now considered a key element in the osteochondral defect healing. The aim of this work was to generate and to evaluate the architecture of three cell-free scaffolds made of collagen, magnesium/hydroxyapatite and collagen hydroxyapatite/wollastonite to be implanted in a sheep animal model. Scaffolds were designed in a bilayer configuration and a novel “Honey” configuration, where columns of hydroxyapatite were inserted within the collagen matrix. The use of different types of scaffolds allowed us to identify the best scaffold in terms of integration and tissue regeneration. The animals included were divided into four groups: three were treated using different types of scaffold while one was left untreated and represented the control group. Evaluations were made at 3 months through CT analysis. The novel “Honey” configuration of the scaffold with hydroxyapatite seems to allow for a better reparative process, although we are still far from obtaining a complete restoration of the defect at this time point of follow-up.

scholarly journals Effect of Bio-Oss® Collagen and Collagen Matrix on Bone Formation

2010 ◽  
Vol 4 (1) ◽  
pp. 71-76 ◽  
Author(s):  
R.W.K Wong ◽  
A.B.M Rabie
Keyword(s):  
Bone Formation ◽  
Collagen Matrix ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Parietal Bone ◽  
Control Group ◽  
Graft Material ◽  
New Bone Formation

Objective: to compare the amount of new bone produced by Bio-Oss® Collagen to that produced by collagen matrix in vivo. Method: eighteen bone defects, 5mm by 10mm were created in the parietal bone of 9 New Zealand White rabbits. 6 defects were grafted with Bio-Oss® Collagen. 6 defects were grafted with collagen matrix alone (positive control) and 6 were left empty (negative control). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Quantitative analysis of new bone formation was made on 100 sections (50 sections for each group) using image analysis. Results: A total of 339% more new bone was present in defects grafted with Bio-Oss® Collagen than those grafted with collagen matrix (positive control). No bone was formed in the negative control group. Conclusion: Bio-Oss® Collagen has the effect of stimulating new bone formation locally compared with collagen matrix in vivo. Bio-Oss® Collagen may be utilized as a bone graft material.

scholarly journals EVALUATION OF THE FUNCTIONAL PROPERTIES OF A BIOLOGICAL CASE FOR IMPLANTABLE CARDIAC DEVICES, IMPREGNATED WITH BIOACTIVE SUBSTANCES IN AN EXPERIMENT ON LARGE LABORATORY ANIMALS

2019 ◽  
Vol 34 (2) ◽  
pp. 118-128
Author(s):  
E. A. Artukhina ◽  
V. A. Vaskovskiy ◽  
A. A. Venediktov ◽  
S. V. Evdokimov ◽  
S. S. Durmanov ◽  
...  
Keyword(s):  
Experimental Study ◽  
Functional Properties ◽  
Collagen Matrix ◽  
Aminocaproic Acid ◽  
Laboratory Animals ◽  
In Vivo Model ◽  
Bioactive Substances ◽  
Cardiac Devices ◽  
Implantable Cardiac Devices

Aim. The purpose of the experimental study was to determine the functional properties of the case with antibacterial and hemostatic preparations impregnated on it, to assess the biological safety and the effectiveness of using this medical device in an in vivo model on large laboratory animals (pigs).Material and Methods. The article presents the first results of an experimental study of a domestic extracellular collagen matrix-based biological case “Bio-NEST” for implantable cardiac devices, impregnated with bioactive substances, in an in vivo experiment on large laboratory animals. The experiment was conducted in three chronological stages to analyze and evaluate the safety, efficacy, and histological compatibility of the biological sheath during implantation and reimplantation of cardiac devices using bioactive substances (antibacterial, hemostatic, and their combinations) as well as under the conditions of the established model of infection with pathogenic microflora implant pocket.Results. Results of the study showed that the use of the experimental cover sample was safe for a laboratory animal. Experimental samples were biocompatible and did not cause inflammatory and immune responses. Implantation of cardiac devices in the sample of the case allowed preventing implant migration and development of infectious inflammation of the implant bed when the experimental cover was impregnated with a solution of rifampicin and aminocaproic acid.

scholarly journals STUDY OF HISTOLOGICAL FEATURES OF LIVER REACTION USING NEW SAMPLES OF POLYMERIC HEMOSTATIC MATERIALS IN THE EXPERIMENT IN VIVO

2020 ◽  
Vol 13 (1) ◽  
pp. 34-40
Author(s):  
Vyacheslav Aleksandrovich Lipatov ◽  
Sergej Viktorovich Lazarenko ◽  
Dmitry Andreevich Severinov ◽  
Anastasia Valeryevna Tarasova
Keyword(s):  
Morphological Structure ◽  
Laboratory Animals ◽  
Control Group ◽  
Central Vein ◽  
Local Means ◽  
Injured Area ◽  
Norm Group ◽  
Histological Characteristics ◽  
Median Laparotomy

Abdominal trauma in 26.7-40.8% of cases is accompanied by trauma of parenchymal organs. The condition of the patient with liver injury in 75% of cases is complicated by the development of hemorrhagic shock. In surgery, to achieve local hemostasis, the most promising method is the topical use of application hemostatic agents, while the composition of such funds determines the effectiveness of hemostasis. The aim of the study is to assess the histological characteristics of the liver using new samples of polymeric hemostatic materials in the experiment in vivo. Materials and methods. As research material was used hemostatic sponge based on natrocarbonatite (Na-CMC) (developed jointly with Linteks, Russia. Saint-Petersburg.) In the experimental groups of laboratory animals under general anesthesia produced a median laparotomy and simulated planar surface injury to the liver. Parenchymal bleeding was stopped by applying application hemostatic materials. An autopsy of the injured area of the liver was performed together with an implanted hemostatic agent. The thickness of the capsule, the size of the Central veins of the liver, the number of dual-core hepatocytes, the size of hepatocytes and their nuclei were measured. Results. The indicator of the area of hepatocytes of groups No. 4 and No. 5 are as close as possible to group No. 1 indicators. Similar to the area of hepatocytes, difference of the area of their kernels in groups No. 6, No. 7 are statistically not significant in comparison with group No. 1. In groups No. 6 and No. 5 the area of kernels is authentically increased, but does not reach values of control group. The greatest statistically significant increase in thickness of the capsule in comparison with group No. 1, group No. 2 and No. 3 was observed in group No. 4. The area of the central vein is authentically increased in comparison with control group in groups No. 2, No. 3 and No. 6. The number of two-nuclear hepatocytes in comparison with norm, group No. 4 and group No. 1 is reliable above in groups No. 6 and No. 7. Conclusion. Histologic reorganizations, changes of morphological structure in places of a trauma and implantation of the tested samples are less expressed when using haemo static implants of experimental groups No. 3-№6 (styptic local means on the basis of Na-KMTs with addition of aminocaproic, acetilsalicylic and aminoacetic acids).

scholarly journals In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1598
Author(s):  
Fernando Bento Cunha ◽  
Karina Torres Pomini ◽  
Ana Maria de Guzzi Plepis ◽  
Virgínia da Conceição Amaro Martins ◽  
Eduardo Gomes Machado ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Repair ◽  
Bone Growth ◽  
Critical Size ◽  
Blood Clot ◽  
Collagen Matrix ◽  
Repair Process ◽  
Biological Behavior ◽  
Control Group

Autologous bone grafts, used mainly in extensive bone loss, are considered the gold standard treatment in regenerative medicine, but still have limitations mainly in relation to the amount of bone available, donor area, morbidity and creation of additional surgical area. This fact encourages tissue engineering in relation to the need to develop new biomaterials, from sources other than the individual himself. Therefore, the present study aimed to investigate the effects of an elastin and collagen matrix on the bone repair process in critical size defects in rat calvaria. The animals (Wistar rats, n = 30) were submitted to a surgical procedure to create the bone defect and were divided into three groups: Control Group (CG, n = 10), defects filled with blood clot; E24/37 Group (E24/37, n = 10), defects filled with bovine elastin matrix hydrolyzed for 24 h at 37 °C and C24/25 Group (C24/25, n = 10), defects filled with porcine collagen matrix hydrolyzed for 24 h at 25 °C. Macroscopic and radiographic analyses demonstrated the absence of inflammatory signs and infection. Microtomographical 2D and 3D images showed centripetal bone growth and restricted margins of the bone defect. Histologically, the images confirmed the pattern of bone deposition at the margins of the remaining bone and without complete closure by bone tissue. In the morphometric analysis, the groups E24/37 and C24/25 (13.68 ± 1.44; 53.20 ± 4.47, respectively) showed statistically significant differences in relation to the CG (5.86 ± 2.87). It was concluded that the matrices used as scaffolds are biocompatible and increase the formation of new bone in a critical size defect, with greater formation in the polymer derived from the intestinal serous layer of porcine origin (C24/25).

scholarly journals Autoplasma as a Hemostatic Agent for Endoscopic Surgery of Hollow Organs

2020 ◽  
Vol 10 (3) ◽  
pp. 212-216
Author(s):  
T. V. Bochkova ◽  
A. Kh. Gainullin
Keyword(s):  
Argon Plasma Coagulation ◽  
Argon Plasma ◽  
Experimental Models ◽  
Laboratory Animals ◽  
Local Administration ◽  
Control Group ◽  
Plasma Coagulation ◽  
Electrosurgical Unit ◽  
Pathological Focus

Introduction. To maximize the effectiveness of hemostatic technologies, it is necessary to optimize local hemostasis through hybrid and controlled approaches, as well as to improve the conditions for tissue surgical dissection preventing perforation of hollow organs. This study is aimed at assessing the efficacy of stopping bleeding and the safety of resection of digestive hollow organs in experimental models of trauma to abdominal organs in laboratory animals.Materials and methods. Experiments were carried out in vivo on 20 rabbits. All animals were divided into 4 experimental groups (5 animals each): I — the control group, in which no methods for stopping bleeding were used; II — the group, in which infiltration of the wall of a hollow organ with saline solution was used; III — the group, in which physical hemostasis was applied using an electrosurgical unit and an argon plasma coagulation apparatus; IV — the group, animals in which underwent controlled local biological hemostasis using autoplasma. Prior to laparotomy, 2–3 ml of whole blood was taken from the rabbit’s ear for preliminary preparation of autoplasma. The prepared autoplasma was introduced into the area of resection or other operation of the mucous membrane of the rabbit’s digestive tract.Results and discussion. Although no statistical difference in the time of stopping bleeding was observed between the control (I) and saline (II) groups, one more episode of bleeding was noted in group II. Preventive local administration of autoplasma (group IV) was established to have a high hemostatic potential. As expected, electrocoagulation was more effective than saline; however, hemostasis achieved by means of argon plasma coagulation is characterized by rapid formation of a necrotic zone, which may lead to undesirable consequences in the long-term period.Conclusion. Preventive local administration of autoplasma and recombinant human protein has a high hemostatic potential in animals. In comparison, electrocoagulation is less effective due to the rapid filling of the pathological focus with blood. 

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