Microbiological, electronic microscopy evaluation of the action of Decasan® and Horosten® on bacteria

2017 ◽  
Vol 23 (1-2) ◽  
pp. 24-30
Author(s):  
V.V . Bobyr ◽  
◽  
O.A. Nazarchuk ◽  
D.V. Paliy ◽  
O.V. Yatsula ◽  
...  
Keyword(s):  
Electronic Microscopy

New Structure of CTAB Templated Silica Films as revealed by GISAXS coupled to HRTEM

2000 ◽  
Vol 628 ◽  
Author(s):  
Sophie Besson ◽  
Catherine Jacquiod ◽  
Thierry Gacoin ◽  
André Naudon ◽  
Christian Ricolleau ◽  
...  
Keyword(s):  
Cetyltrimethylammonium Bromide ◽  
Grazing Incidence ◽  
Hexagonal Structure ◽  
X Ray Diffraction ◽  
Electronic Microscopy ◽  
Silica Films ◽  
X Ray ◽  
X Ray Scattering ◽  
Hexagonal Arrangement ◽  
Spherical Micelles

ABSTRACTA microstructural study on surfactant templated silica films is performed by coupling traditional X-Ray Diffraction (XRD) and Transmission Electronic Microscopy (TEM) to Grazing Incidence Small Angle X-Ray Scattering (GISAXS). By this method it is shown that spin-coating of silicate solutions with cationic surfactant cetyltrimethylammonium bromide (CTAB) as a templating agent provides 3D hexagonal structure (space group P63/mmc) that is no longer compatible with the often described hexagonal arrangement of tubular micelles but rather with an hexagonal arrangement of spherical micelles. The extent of the hexagonal ordering and the texture can be optimized in films by varying the composition of the solution.

Evidence of Browning of White Adipocytes in Poorly Survived Fat Grafts in Patients

2021 ◽  
Author(s):  
Tong Liu ◽  
Su Fu ◽  
Qian Wang ◽  
Hao Cheng ◽  
Dali Mu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Uncoupling Protein ◽  
Fold Increase ◽  
Fat Grafting ◽  
Electronic Microscopy ◽  
Uncoupling Protein 1 ◽  
Fat Transfer ◽  
Flap Transfer ◽  
Fat Grafts ◽  
White Adipocytes

Abstract Background Browning adipocytes induced by burn and cancer were assumed less viable and more prone to necrosis for their hypermetabolic properties. Recent studies have shown browning of white adipose after fat engraftment in mice. Objectives We tend to evaluate whether fat transfer could induce browning biogenesis in fat grafts in humans and if it is associated with graft necrosis. Methods Necrotic adipose grafts were excised from 11 patients diagnosed with fat necrosis after fat grafting or flap transfer. Non-necrotic fat grafts were from 5 patients undergoing revisionary surgeries after flap transfer. Histology and electronic microscopy, protein and gene expression of browning related marker analyses were performed. Results Fat grafts with necrosis demonstrated a higher gene expression level of uncoupling protein-1 (>5-fold increase, **p<0.01), a master beige adipocyte marker, than non-necrotic fat grafts. Electronic microscopy and histology showed that browning adipocytes were presented in necrotic adipose in patients. Conclusions Fat transfer induced browning adipocytes in patients and was evident in patients with post grafting necrosis.

Browning of White Adipocytes in Fat Grafts Associated with Higher Level of Necrosis and Type 2 Macrophages Recruitment

2021 ◽  
Author(s):  
Tong Liu ◽  
Su Fu ◽  
Qian Wang ◽  
Hao Cheng ◽  
Dali Mu ◽  
...  
Keyword(s):  
Fold Increase ◽  
Chow Diet ◽  
Sham Operation ◽  
Type I ◽  
Electronic Microscopy ◽  
Fat Transfer ◽  
Fat Grafts ◽  
Female Nude ◽  
Normal Chow

Abstract Background Induced browning adipocytes were assumed less viable and more prone to necrosis for their hypermetabolic property. Our previous study showed that browning of adipocytes was more evident in fat grafts with necrosis in humans. Objectives We aimed to estimate whether fat-transfer-induced browning biogenesis was associated with necrosis and its potential inflammation mechanisms in murine models. Methods Human subcutaneous adipose from thigh or abdomen of 5 patients via liposuction were injected in 100µl or 500µl (n=20 per group) into the dorsal flank of 6-8-week female nude mice fed with normal chow diet, and harvested after 2, 4, 8 and 12 weeks. Control groups did not receive any grafting procedures (sham operation), where lipoaspirates were analyzed immediately after harvest. Histology and electronic microscopy, immunological analyses of browning markers, necrosis marker, and type I/II macrophages markers in mice were performed. Results Histology and electronic microscopy showed browning adipocytes in in fat grafts with higher level of necrosis (0.435±0.017pg/ml for cleaved caspase-3, **p<0.01), IL-6(749.0±134.1pg/ml,***p<0.001) and infiltration of type 2 macrophage profiles in mice(2-fold increase, *p<0.05). Conclusions Browning of adipocytes induced by fat transfer in mice is in parallel with post-grafting necrotic levels, associated with elevated IL-6 and activated M2 macrophages profiles which promote browning development.

Microstructure and Properties of a Non-Quenched Prehardened Steel at Different Cooling Rate

2012 ◽  
Vol 524-527 ◽  
pp. 1976-1979
Author(s):  
Yi Luo ◽  
Jin Ming Peng
Keyword(s):  
Mechanical Properties ◽  
Fine Structure ◽  
Optical Microscopy ◽  
Cooling Rate ◽  
Retained Austenite ◽  
Transformation Products ◽  
Electronic Microscopy ◽  
Transmission Electronic Microscopy ◽  
Microstructure And Properties ◽  
Cooling Conditions

Mechanical properties of non-quenched prehardened (NQP) steel air cooled and sand cooled after forged were tested and their microstructure was investigated by optical microscopy and transmission electronic microscopy(TEM). The results show that mechanical properties of the NQP steel are similar at both cooling conditions, and their microstructure is bainite, whose fine structure is main bainite ferrite laths, retained austenite films, retained austenite islands and their transformation products. Bainite ferrite laths of the NQP steel air cooled are narrower than that sand cooled, while more retained austenite islands exist in latter.

Hydrothermal Treatment of Coprecipitated YSZ Powders

2010 ◽  
Vol 660-661 ◽  
pp. 983-988 ◽  
Author(s):  
Alexander Rodrigo Arakaki ◽  
Walter Kenji Yoshito ◽  
Valter Ussui ◽  
Dolores Ribeiro Ricci Lazar
Keyword(s):  
Surface Area ◽  
Hydrothermal Treatment ◽  
Gas Adsorption ◽  
X Ray Diffraction ◽  
Electronic Microscopy ◽  
Immersion Method ◽  
Cubic Symmetry ◽  
X Ray ◽  
Granulometric Analysis ◽  
Symmetry Surface

Zirconia stabilized with 8.5 mol% yttria (YSZ) were synthesized by coprecipitation and resulting gels were hydrothermallly treated at 200°C and 220 PSI for 4, 8 and 16 hours. Products were oven dried at 70°C for 24 hours, isostatically pressed as pellets and sintered at 1500 °C for 1 hour. Powders were characterized for surface area with N2 gas adsorption, X-ray diffraction, laser diffraction granulometric analysis and scanning and transmission electronic microscopy. Density of ceramics was measured by an immersion method based on the Archimedes principle. Results showed that powders dried at 70°C are amorphous and after treatment has tetragonal/cubic symmetry. Surface area of powders presented a significant reduction after hydrothermal treatment. Ceramics prepared from hydrothermally treated powders have higher green density but sintered pellets are less dense when compared to that made with powders calcined at 800°C for 1 hour due to the agglomerate state of powders. Solvothermal treatment is a promising procedure to enhance density.

Influence of Cooling Speed on the Microstructure of Particles Reinforced 7055Al Composites Subject to Regulatory Cryogenic Treatment

2014 ◽  
Vol 941-944 ◽  
pp. 314-317
Author(s):  
Guirong Li ◽  
Hong Ming Wang ◽  
Yu Hua Cui ◽  
Yue Ming Li ◽  
Cong Xiang Peng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Dislocation Density ◽  
Microstructural Evolution ◽  
Cryogenic Treatment ◽  
Preferred Orientation ◽  
Electronic Microscopy ◽  
Transmission Electronic Microscopy ◽  
Cooling Speed

Al3Ti and Al3Zr particles reinforced 7055Al composites were processed by cryogenic treatment with different cooling speed at 1°C/min, 3°C/min and 5°C/min. Transmission Electronic Microscopy (TEM) was mainly used to analyze the microstructural evolution of the treated samples. The results show that with the increase of cooling speed the precipitate amount and dislocation density have been increased. The precipitates orientation exhibits some preferred orientation. The mechanical properties test demonstrates that for the samples treated at 5°C/min the tensile strength and elongation has arrived at the utmost.

scholarly journals Tribological Performance of CoCrMo Alloys with Boron Additions in As-Cast and Heat-Treated Conditions

Metals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 355
Author(s):  
Marco A. L. Hernandez-Rodriguez ◽  
Diego E. Lozano ◽  
Gabriela M. Martinez-Cazares ◽  
Yaneth Bedolla-Gil
Keyword(s):  
Boron Content ◽  
Tribological Performance ◽  
Secondary Phases ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Hardness Tests ◽  
Heat Treated ◽  
Cocrmo Alloys ◽  
Cast Condition ◽  
Ball On Disc

The present study evaluates the effect of boron additions on the tribological performance of CoCrMo alloys. The alloys were prepared with boron ranging from 0.06 to 1 wt%. The materials were characterized using metallographic techniques, scanning electronic microscopy, and roughness and hardness tests. Tribological evaluation was made by means of ball-on-disc tests for sliding distances of 4, 8 and 12 km. The samples were in the as-cast condition and after a heat treatment at 1200 °C for 1 h, finished by water quenching. The results showed that wear resistance was influenced by the microstructure and the number of secondary phases. The volume loss decreased as the boron content increased. Due to hard phases, abrasion wear was observed. Delamination fatigue was also detected after long sliding distances. Both wear mechanisms diminished in higher boron content alloys.

Ag-TiO2 Nanocomposites: visible or solar light driven plasmonic photocatalysis?

2021 ◽  
Vol 02 ◽  
Author(s):  
Larissa Bach-Toledo ◽  
Patricio G. Peralta-Zamora ◽  
Liziê Daniela Tentler Prola
Keyword(s):  
Photocatalytic Activity ◽  
Solar Radiation ◽  
Noble Metals ◽  
Halogen Lamp ◽  
Tio2 Surface ◽  
Electronic Microscopy ◽  
Visible Radiation ◽  
Artificial Uv ◽  
Positive Effect

Background: The demand for photocatalytic processes assisted by solar radiation has stimulated the upgrading of established systems, as the semiconductor modification with noble metals. Objective: the synthesis, characterization, and photocatalytic activity evaluation of the Ag-TiO2, against sulfamethoxazole molecule, and investigate the significance of the plasmonic phenomenon in Visible (450 - 1000nm) and UV-Vis (315-800 nm) radiation. Methods: Different nanocomposites Ag/TiO2 ratios were synthesized by the deposition of Ag nanoparticles on the TiO2 surface by in-situ photoreduction, and then calcinated at 400°C for 2 hr. The chemical-physical properties of the materials were examined by UV-Vis Diffuse Reflectance (UV-Vis DRS) Scanning Electronic Microscopy (SEM), Transmission Electronic Microscopy (TEM), X-Ray Energy Dispersive Spectroscopy (EDS). The experiments were conducted in a cooled photochemical reactor irradiated by halogen lamp (250W). The degradation of Sulfamethoxazole was monitored by HPLC-DAD. Results: Although the prepared photocatalysts show an intense plasmonic band centered at 500 nm, no photocatalytic activity was observed in the process assisted by artificial visible radiation ( ≥ 450 nm). In processes assisted by artificial UV-Vis radiation, the photolysis rate of the model compound (sulfamethoxazole) was higher than the photocatalytic rate, and in the absence of UV radiation, all the reactions were inhibited. The positive effect of the presence of silver nanoparticles onto the TiO2 surface was only evidenced in studies involving solar radiation. Conclusion: The results suggest the need for a balance between UV and Vis radiation to activate the nanocomposite and perform the sulfamethoxazole degradation.

Enigmatic mycoplasma colonies: the analisis by scanning and transmission electronic microscopy

2021 ◽  
Vol 39 (3) ◽  
pp. 10
Author(s):  
I.V. Rakovskaya ◽  
S.G. Andreevskaya ◽  
O.I. Barkhatova ◽  
G.A. Levina ◽  
L.G. Gorina ◽  
...  
Keyword(s):  
Electronic Microscopy ◽  
Transmission Electronic Microscopy
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