scholarly journals Induksi Kalus Tanaman Puspa (Schima wallichii (DC.) Korth) dengan Penambahan Beberapa Konsentrasi Benzyl Amino Purin (BAP) dan 2,4-Diklorofenoksiasetat (2,4-D)

2019 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Husri Meli ◽  
Zozy Aneloi Noli ◽  
Suwirmen Suwirmen
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Design Method ◽  
Randomized Design ◽  
Schima Wallichii ◽  
Completely Randomized Design ◽  
Department Faculty ◽  
Benzyl Amino Purine

The research about Callus Induction of Puspa (Schima wallichii (DC.) Korth) with several concentrations addition of Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxyacetid acid  (2,4-D) had been done from October until November 2016 at Plant Physiology dan Tissue Culture Laboratory, Biology Department, Faculty of Mathematics and Natural Science, Andalas University. The aim of this researh is to get the combination of 2,4-D and BAP to induce the best callus of Schima wallichii. This research used a Completely Randomized Design Method with 10 treatments and 3 replications. The result showed that combination of 2 ppm 2,4-D + 0,75 ppm BAP was the best concentration to induce callus of Schima wallichii.

scholarly journals Induksi Kalus Kentang Asal Desa Dombu (Solanum tuberosum L.) Dengan Zpt Indole-3-Acetic Acid (IAA)

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Lyly Zulraufianti ◽  
Asri Pirade Paserang
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Solanum Tuberosum ◽  
Natural Science ◽  
Callus Induction ◽  
Solanum Tuberosum L ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Department Faculty ◽  
Indole 3 Acetic Acid

The research of callus induction in potato (S. tuberosum  L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that  the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.

scholarly journals Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D)

2019 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Azharia Khalida ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Natural Science ◽  
Callus Induction ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Greenish Yellow ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.

scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

scholarly journals Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

2020 ◽  
Vol 22 (1) ◽  
pp. 39
Author(s):  
Amalina Wahyuni ◽  
Benni Satria ◽  
Aprizal Zainal
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Economic Value ◽  
Market Demand ◽  
Compact Structure ◽  
Randomized Design ◽  
Aquilaria Malaccensis ◽  
Completely Randomized Design ◽  
Range Test

<p>Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact.  Yellow callus and compact structure could be utilized for organogenesis.</p>

scholarly journals Induksi kalus Artemisia vulgaris L. dengan Pemberian Beberapa Konsentrasi 2,4-Dichlorophenoxyacetic Acid(2,4-D)

2015 ◽  
Vol 4 (4) ◽  
pp. 216
Author(s):  
Nazhira - Fadhilah ◽  
Zozy Aneloi Noli ◽  
Suwirmen - Suwirmen
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Effective Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Artemisia Vulgaris ◽  
Fresh Weight ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Physiology Laboratory

The research about callus induction Artemisia vulgaris L. by giving several concentration 2,4-Dichlorophenoxyacetic acid (2,4-D), has been done from May to August 2015 in Plant Physiology Laboratory and Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Andalas. The aim of this studywas found the effective concentration of 2,4-D to induce callus of A. vulgaris. The research used Completely Randomized Design (CRD) with 7 treatments and 4 replications. The treatments were : without 2,4-D (control); 0.25 mg/L 2,4-D; 0.50 mg/L 2,4-D; 0.75 mg/L 2,4-D; 1.00 mg/L 2,4-D; 1.25 mg/L 2,4-D; 1.5 mg/L 2,4-D. The result showed that 0.25-1,5 mg/L 2,4-D were able induction callus of A. vulgaris, withcompactuntilthefriabletexture, color ofthe resultingcallusisyellowish green, brownish-green, yellow-brown, whiteyellowishandgreenishwhite. 2,4-D 1.5mg/Lwas the best concentrationto increase fresh weight of callus.

scholarly journals Pengaruh Konsentrasi Giberelin (GA3) terhadap Pertumbuhan Kailan (Brassica oleracea L. Var alboglabra) pada Berbagai Media Tanam dengan Hidroponik Wick System

2018 ◽  
Vol 6 (2) ◽  
pp. 63
Author(s):  
Asih Maharani ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Brassica Oleracea ◽  
Natural Science ◽  
Growth And Yield ◽  
Randomized Design ◽  
Chinese Kale ◽  
Accelerate Growth ◽  
Completely Randomized Design ◽  
Physiology Laboratory ◽  
Brassica Oleracea L

Effect of giberelin (GA3) concentration on growth and yield of Chinese kale (Brassica oleracea L. Var alboglabra) in various medium using hydroponic wick system . This research conducted from April until July 2016 in Wire House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University. The purposes of this research were to obtain GA3 concentration and planting medium that accelerate growth and yield of Chinese kale and determine the interaction between GA3 and planting medium to the gowth and yield of Chinese kale planted using hydroponics wick system. This research used Completely Randomized Design (CRD) in factorial with three replications, as the first factor were concentration of GA3 (0 ppm, 20 ppm, 40 ppm and 60 ppm) and the second factor were medium (zeolite, sand, husk and sawdust). The best result in accelerated the growth of Chinese kale was the treatment at the concentration of 60 ppm GA3 and zeolite was suitable medium to Chinese kale growth. This research found the interaction between GA3 and medium on the length of the roots Chinese kale used hydroponic wick system.

scholarly journals Pengaruh Naphthalene Asam Asetat (NAA) pada Pertumbuhan Akar Pisang Raja Kinalun Secara In Vitro

2018 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Rahmi Rini Dwi Putri ◽  
Suwirmen Suwirmen ◽  
Nasril Nasir
Keyword(s):  
Plant Physiology ◽  
Tissue Culture ◽  
Root Growth ◽  
Natural Sciences ◽  
Effective Concentration ◽  
Randomized Design ◽  
Effective Doses ◽  
Completely Randomized Design

The influence of Naphthalene Acetate Acid (NAA) on the in vitro root growth of banana Raja Kinalun was carried out from April to September 2015 in The Laboratories of Plant Physiology and Tissue Culture, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang. The aim of  this study was to find the effective concentration of NAA for initiation of root. This study used a Completely Randomized Design (CRD) with four treatments and three replications. The treatments were: without NAA (control); NAA 1 ppm, NAA 2 ppm and NAA 3 ppm. The result showed that the effective doses were NAA 1 and 2 ppm for each number of roots growth.

scholarly journals The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in Various Planting Medium

2019 ◽  
Vol 7 (2) ◽  
pp. 91
Author(s):  
Suci Rahmadani Putri ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli
Keyword(s):  
Plant Physiology ◽  
Natural Science ◽  
Root Length ◽  
Coconut Fiber ◽  
Randomized Design ◽  
Ant Nest ◽  
Completely Randomized Design ◽  
Physiology Laboratory

The research about The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in various planting medium was held from May until August 2018 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was to find out the effects of various planting medium to the growth of shoot cutting Ant-nest Plant. This research used Completely Randomized Design (CRD) with five treatments (sand, husk charcoal, media moss, coconut fiber and fern’s root) and six replications. The results showed that shoot cutting that planted in sand, husk charcoal and fern had a highest life percentage (100%). Shoot cutting that planted in coconut fiber showed a highest height accretion. Shoot cutting that planted in media moss showed the highest root amount, longest root length and containing clorophyl level.

scholarly journals KONSENTRASI IAA (Indole Acetic Acid) DAN BAP (Benzyl Amino Purine) PADA KULTUR JARINGAN ULIN (Eusideroxylon zwageri)

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Herni Wahyuni ◽  
Reine Suci Wulandari ◽  
Muflihati Muflihati
Keyword(s):  
Tissue Culture ◽  
Indole Acetic Acid ◽  
Economic Value ◽  
Culture Technique ◽  
Optimum Concentration ◽  
Randomized Design ◽  
In The Wild ◽  
Completely Randomized Design ◽  
Benzyl Amino Purine ◽  
Eusideroxylon Zwageri

Ulin (Eusideroxylon zwageri) is a species of wood with high economic value that is difficult to find in the wild. Regeneration of E. zwageri is naturally very slow. Efforts to increase the procurement of E. zwageri seedlings can be done with a tissue culture technique. Through tissue culture, the seeds obtained will be uniform, have better quality and faster. This study aims to determine the effect and optimum concentration of IAA and BAP growth regulators on E. zwageri explants growth. The method used is experimental using a completely randomized design. Treatment factors are IAA concentration factor with 5 levels of treatment (0 mg/L, 0.5 mg/L, 1 mg/L, 1.5 mg/L, and 2 mg/L) and BAP concentration of 5 treatment levels (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, and 4 mg/L). Each treatment was repeated 3 times. The results showed that the treatment of IAA concentration, BAP concentration and the interaction of IAA and BAP concentration affected the swelling of explants and growth of E. zwageri callus. The best concentration for callus explants was IAA of 2 mg/L and BAP of 0 mg/L, with a time of 12-13 weeks after planting.Keywords: BAP, E. zwageri, IAA, Tissue Culture

scholarly journals Pengaruh Jenis dan Konsentrasi Bahan Pemlastis terhadap Bioplastik Glukomanan

2021 ◽  
Vol 9 (1) ◽  
pp. 75
Author(s):  
Aditya Nandika A.J ◽  
Bambang Admadi Harsojuwono ◽  
I Wayan Arnata
Keyword(s):  
Tensile Strength ◽  
Polyethylene Glycol ◽  
Design Method ◽  
Elongation At Break ◽  
Method Factor ◽  
Randomized Design ◽  
Multiple Comparison Test ◽  
Completely Randomized Design ◽  
Time Variables ◽  
Degradation Time

This research aims to determine the effect of plasticizer types and concentrations on the characteristics of glucomannan bioplastics, and to determine the types and concentrations of plasticizers that can produce glucomannan bioplastics with the best characteristics. This experimental design used a completely randomized design method. Factor I is a type of plasticizer consisting of glycerol, sorbitol, propanol-2, and polyethylene glycol. The second factor is the concentration of plasticizers which consists of 4 levels, namely 0.5%: 1.5%: 2.5%: 3.5%. The experiment resulted in 16 treatment combinations and grouped into 2 groups to obtain 32 experimental units. The data were analyzed for their diversity and continued with the Duncan multiple comparison test. The results showed that the type and concentration of plasticizers had a very significant effect on tensile strength, elongation at break, modulus young, and swelling. The interaction has a very significant effect on tensile strength and expansion and has a significant effect on the elasticity of glucomannan bioplastics. Meanwhile, the type and concentration of plasticizers had no significant effect on the length of biodegradation. The best glucomannan bioplastic was obtained in the treatment of glycerol plasticizers with a concentration of 1.5 % with a tensile strength value of 6.17 MPa, elongation at break of 21.50 %, elasticity 28.72 MPa development 25.84 %, and degradation time of 8 days. Bioplastics produced in this study have meet the SNI 7188.7:2016 standards in the elongation test at break and standards ASTM 5336 in the degradation time variables. The resulting bioplastic has not met SNI on the tensile strength, modulus young, and swelling variables. Keywords : bioplastic, glucomannan, glycerol, polyethylene glycol, propanol-2, sorbitol

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