scholarly journals The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in Various Planting Medium

2019 ◽  
Vol 7 (2) ◽  
pp. 91
Author(s):  
Suci Rahmadani Putri ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli

The research about The Growth of Shoot Cutting of Ant-nest Plant (Myrmecodia pendens Merr. & L.M. Perry) that Planted in various planting medium was held from May until August 2018 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was to find out the effects of various planting medium to the growth of shoot cutting Ant-nest Plant. This research used Completely Randomized Design (CRD) with five treatments (sand, husk charcoal, media moss, coconut fiber and fern’s root) and six replications. The results showed that shoot cutting that planted in sand, husk charcoal and fern had a highest life percentage (100%). Shoot cutting that planted in coconut fiber showed a highest height accretion. Shoot cutting that planted in media moss showed the highest root amount, longest root length and containing clorophyl level.

2018 ◽  
Vol 6 (2) ◽  
pp. 63
Author(s):  
Asih Maharani ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli

Effect of giberelin (GA3) concentration on growth and yield of Chinese kale (Brassica oleracea L. Var alboglabra) in various medium using hydroponic wick system . This research conducted from April until July 2016 in Wire House and Plant Physiology Laboratory, Department of Biology, Faculty of  Mathematics and Natural Science, Andalas University. The purposes of this research were to obtain GA3 concentration and planting medium that accelerate growth and yield of Chinese kale and determine the interaction between GA3 and planting medium to the gowth and yield of Chinese kale planted using hydroponics wick system. This research used Completely Randomized Design (CRD) in factorial with three replications, as the first factor were concentration of GA3 (0 ppm, 20 ppm, 40 ppm and 60 ppm) and the second factor were medium (zeolite, sand, husk and sawdust). The best result in accelerated the growth of Chinese kale was the treatment at the concentration of 60 ppm GA3 and zeolite was suitable medium to Chinese kale growth. This research found the interaction between GA3 and medium on the length of the roots Chinese kale used hydroponic wick system.


2019 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Husri Meli ◽  
Zozy Aneloi Noli ◽  
Suwirmen Suwirmen

The research about Callus Induction of Puspa (Schima wallichii (DC.) Korth) with several concentrations addition of Benzyl Amino Purine (BAP) and 2,4-Dichlorophenoxyacetid acid  (2,4-D) had been done from October until November 2016 at Plant Physiology dan Tissue Culture Laboratory, Biology Department, Faculty of Mathematics and Natural Science, Andalas University. The aim of this researh is to get the combination of 2,4-D and BAP to induce the best callus of Schima wallichii. This research used a Completely Randomized Design Method with 10 treatments and 3 replications. The result showed that combination of 2 ppm 2,4-D + 0,75 ppm BAP was the best concentration to induce callus of Schima wallichii.


2015 ◽  
Vol 4 (4) ◽  
pp. 216
Author(s):  
Nazhira - Fadhilah ◽  
Zozy Aneloi Noli ◽  
Suwirmen - Suwirmen

The research about callus induction Artemisia vulgaris L. by giving several concentration 2,4-Dichlorophenoxyacetic acid (2,4-D), has been done from May to August 2015 in Plant Physiology Laboratory and Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Science, University of Andalas. The aim of this studywas found the effective concentration of 2,4-D to induce callus of A. vulgaris. The research used Completely Randomized Design (CRD) with 7 treatments and 4 replications. The treatments were : without 2,4-D (control); 0.25 mg/L 2,4-D; 0.50 mg/L 2,4-D; 0.75 mg/L 2,4-D; 1.00 mg/L 2,4-D; 1.25 mg/L 2,4-D; 1.5 mg/L 2,4-D. The result showed that 0.25-1,5 mg/L 2,4-D were able induction callus of A. vulgaris, withcompactuntilthefriabletexture, color ofthe resultingcallusisyellowish green, brownish-green, yellow-brown, whiteyellowishandgreenishwhite. 2,4-D 1.5mg/Lwas the best concentrationto increase fresh weight of callus.


2019 ◽  
Vol 7 (2) ◽  
pp. 109
Author(s):  
Azharia Khalida ◽  
Suwirmen Suwirmen ◽  
Zozy Aneloi Noli

The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.


2019 ◽  
Vol 6 (2) ◽  
pp. 268
Author(s):  
Tressa Pratywi Gupitasari ◽  
Zozy Aneloi Noli ◽  
Suwirmen Suwirmen

The research about roots induction and growth of shoot cutting of Jirak (Eurya acuminata DC.) by adding some growth regulator solutions in various planting medium, was conducted from May until August 2017 in Greenhouse and Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University. The aims of the research to find out effect of  providing growth regulator solution with different types, used of planting medium and interaction of both factors the best to roots induction and growth Jirak (E. acuminata DC.) by cutting. The research used Complete Randomized Design (CRD) factorial with two factors and three replications. Factors A were  growth regulator solution (a0: control, a1: IBA 100 ppm, a2: NAA 100 ppm, a3: IAA 100 ppm) and factors B were various planting medium (b0: garden soil, b1: sand, b2: charcoal husk). The research result were adding some growth regulator solutions and various planting medium significanly affected roots induction and growth of jirak and there is interaction on the high of plant. The treatment of 100 ppm IAA and treatment using sand medium obtain the highest result in increasing roots induction and growth of shoot cutting of Jirak.


2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 


Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.


2017 ◽  
Vol 18 (02) ◽  
pp. 62-68
Author(s):  
Retno Prihatini

The research has been conducted to find out the best concentration of coconut water in the induction of roots of cuttings axillary buds Andrographis paniculata Nees. This research was conducted in Plant Physilogy of Division,   Department of Biology, Faculty of Math and Natural Science, AndalasUniversity, for 12 weeks.  The research used Completely Randomized Design. The material used was axillary buds  A.  paniculata  with nodal number 3. treated for 30 minutes in coconut water 10, 15 and 20% and aquadest as a controle each with 6 replications. Observations were done on live percentage, root length, root  numerous and  cuttings  height  after 12 weeks planting. The results showed that the  concentration 10 to 15% of coconut water was able with significant  to induce the  root growth of cutting axillary buds A. Paniculat.  The concentration 15% of coconut water was the best in inducing  cutting height growth, each with an average root length is 8.2 cm and the average number of roots that formed is 16, 0 and the average cutting height is  22.7 cm. There was an increase of average of  cuttings height growth by 86% which was supported by the average increase of root length and root number of 105.0% and 280.9% on the occasion of cuttings of axillary buds in coconut water concentration 15%.


2020 ◽  
Vol 12 (3) ◽  
pp. 365-371
Author(s):  
Endalkachew Baye ◽  
Temesgen Matewos ◽  
Derbew Belew

In vitro rooting of micropropagated shoots were carried out with the aim of evaluating the root induction responses of two tomatoes (Lycopersicon esculentum MILL) varieties (Gelilema and Chali) using Indole -3- butyric acid (IBA). Seven levels of IBA (0.0, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 mg L-1) were used in a completely randomized design (CRD) in factorial combinations (seven level of IBA*two varieties) with three replications.  After the plantlets were kept in the rooting media for three weeks, data on rooting percentage, number of roots/shoots and root length in cm were collected. The analysis of variance showed that the interaction of IBA*Var was highly significantly different for rooting percentage, a number of roots/shoot and root length at p<0.01. The highest rooting percentage (100.00±0.00), number of roots/shoot (14.20±0.35) and root length (10.7±0.29) were received from Chali on free Murashige and Skoog medium (MS). At the same time, the lowest percentage of rooting (11.11±0.00), number of roots/shoot (0.887±0.19) and root length (1.00±0.00 cm) were obtained from Gelilema on MS+1.5mg/l IBA. For acclimatization, the in vitro rooted shoots were transplanted into plastic pots containing a mixture of oven sterilized soil and sand at a ratio of 2:1. After three weeks, a survival rate of 67.7% for Chali and 58.1% for Gelilema was obtained. From the above result, it can be concluded that free MS medium was the best for in vitro rooting of the two tomato varieties. The optimized protocol will be useful for rapid in vitro multiplication of the two tomato varieties.


2021 ◽  
Vol 17 (2) ◽  
pp. 09-15
Author(s):  
Neisiany Rebelo Pimentel ◽  
Daniela Pauletto ◽  
Àdria Fernandes da Silva ◽  
Thiago Gomes da Silva Oliveira ◽  
Orlanilson da Silva Brito

Moringa oleiferaLam., known as moringa, is an arboreal plant belonging to the Moringaceae family, native to India,and has a high capacity to adapt to climatic conditions and arid soils. In this study we evaluated the initial development of seedlings of M. oleiferaLam. under different substrates conditions. The experiment was conducted in a completely randomized design, consisting of eight treatments resulting from combinations of the base substrate (85% soil, 3% carbonized rice straw and 12% coconut fiber). At 60 days after sowing, plant height, stem diameter, dry mass of the aerial part and root system, total dry mass were evaluated, in addition to the Dickson quality index (IQD). The best seedling growth occurred using the combination of 20% poultry litter and 10% bovine manure. Seedlings produced with substrates formulated with 20% poultry litter and 10% bovine manure showed gains in the evaluated variables, which could be a viable alternative for the production of moringa seedlings.


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