scholarly journals The Direct Organogenesis In Local Clones Of Patchouli Plant( Pogostemon cablin Benth)InVitro

2020 ◽  
Vol 3 (1) ◽  
pp. 16-19
Author(s):  
Reni Mayerni
Keyword(s):  
Tissue Culture ◽  
Direct Organogenesis ◽  
Research Material ◽  
Pogostemon Cablin ◽  
West Sumatra

Background and Objective: The purpose of this research is to get to be able to identify local patchouli plants that are able to produce the best plantlets in many concentrations of BAP. Materials and Methods: The research conducted in Laboratory of Tissue Culture Faculty of Agriculture, Andalas University from May until November 2019. The research material used in this study was the accession of patchouli plants in West Sumatra, namely: Situak and method of research is completely randomized design.Results: Concentrations of 0.5 ppm kinetin + 0.5 ppm BAP, 0.5 ppm kinetin + 1 ppm BAP and 1 ppm kinetin + 1 ppm BAP can stimulate organogenesis (bud) in vitro

DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

scholarly journals Coconut Tissue Culture: The Indian Initiatives, Experiences and Achievements

CORD ◽  
2017 ◽  
Vol 33 (2) ◽  
pp. 11
Author(s):  
Anitha Karun
Keyword(s):  
Tissue Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Zygotic Embryo ◽  
Shoot Meristem ◽  
Direct Organogenesis ◽  
Zygotic Embryos ◽  
Immature Inflorescence ◽  
High Yielding Varieties

Coconut is one of the principal crops of India cultivated in over 35 districts mainly in the southern states. The productivity of the crop is declining in many of the traditionally cultivated regions owing to ageing plantations as well as biotic and abiotic stresses. These plantations are to be replanted with high yielding varieties/hybrids for which adequate quantity of quality planting material is not available. Even though tissue culture research was initiated in many laboratories in the country, the work was eventually phased out in most of the laboratories for want of a repeatable protocol.  At ICAR-CPCRI, coconut tissue culture programs have been continuing for the past three decades. The attempts made include experimentation with different explants viz., immature inflorescence, plumular tissues, mature palm shoot meristem, ovary and anthers and different culture media supplemented with varying levels and types of hormones. Some of the successful protocols developed at the Institute include coconut zygotic embryo culture for collection and exchange of germplasm, cryopreservation and retrieval of zygotic embryos and pollen and plantlet regeneration from plumular tissues. Even though ICAR-CPCRI has succeeded in obtaining plantlets via direct organogenesis from inflorescence explants, the absence of friable calli formation from explants, the low rate of somatic embryo formation, large number of cultures turning to abnormal shoot development, non conversion of somatic embryos into plantlets, and formation of abnormal somatic embryos remain the major bottlenecks. Gene expression studies are being currently undertaken to decipher the molecular basis of in vitro recalcitrance.

scholarly journals (79) Direct Bulblet Regeneration from Mature Embryo: A Rapid, Efficient and Genotype-independent In Vitro Morphogenesis Pathway for Preservation of Endangered Wild Populations of Fritillaria imperialis and Fritillaria persica

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1066C-1066
Author(s):  
Manijeh Mohammadi-Dehcheshmeh ◽  
Ahmad Khalighi ◽  
Esmaeil Ebrahimie ◽  
Manoochehr Sardari ◽  
Rohangiz Naderi
Keyword(s):  
Tissue Culture ◽  
Mature Embryo ◽  
Direct Organogenesis ◽  
Wild Populations ◽  
In Vitro Morphogenesis ◽  
Culture Techniques ◽  
Mature Embryos ◽  
Best Response ◽  
Heterozygous Plant

Wild populations of Fritillaria sp. have dramatically decreased in Iran because of pest overflow and continual grazing. Previous studies have shown that Fritillaria cannot rapidly and efficiently propagate by traditional methods. In vitro tissue culture techniques have shown high potential for micropropagation of endangered plants. The use of bulb-scale pieces for tissue culture can result in the destruction of the endangered parent plant. Fritillaria is a heterozygous plant in which the genetic content of each embryo is different from others, even on the same plant. In this study, mature embryos of F. imperialis and F. persica were used as explant for the first time. Embryos were extracted from seeds and cultured on B5 medium supplemented with various combinations of BAP (0, 0.1, 1 mg/L), NAA (0, 0.4, 4 mg/L), and IAA (0, 0.4, 4 mg/L). Embryo explant showed low genotype dependency between different heterogenous and heterozygote populations of both F. imperialis and F. persica. The best response of bulblet regeneration in both F. imperialis and F. persica was obtained from 1 mg/L BAP + 0.4 mg/L NAA+4 mg/L IAA and direct organogenesis pathway, with 15 bulblets per explant for F. imperialia and 20 for F. persica. Because of the large number of embryos in a plant and their different genetic contents, established in vitro propagation by using embryo explant in this study can provide broad genetic resources and variations. As explained above, in vitro protocols can play a major role in rescuing F. imperialis and F. persica from extinction.

scholarly journals MULTIPLIKASI TANAMAN NILAM (Pogostemon cablin Benth) var. TAPAK TUAN SECARA IN VITRO

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
SITI ASIYAH ◽  
CHRISTIANI TUMILISAR ◽  
TUTI LESTARI
Keyword(s):  
Tissue Culture ◽  
Leaf Explants ◽  
Stem Explants ◽  
Plant Propagation ◽  
In Vitro Techniques ◽  
Pogostemon Cablin ◽  
Shoot Number ◽  
Independent Variable ◽  
Range Test

ABSTRACTTissue culture is a technique widely used for propagation and improvement of crop quality. One way of plant propagation can be done with in vitro techniques. The purpose of the study was to determine the combinations and concentrations of NAA and BAP are appropriate for plant propagation patchouli. Dependent variable is the time of initiation of callus and shoots, callus texture, color callus, shoot number, and the number of roots patchouli. Independent variable is the concentration of growth regulators NAA and BAP. The method in this study is the experimental method. Data were analyzed by two-way Anova test followed by Duncan’s Multiple Range Test (DMRT). The results showed the stem explants concentrations of NAA and BAP best to induce callus is without NAA and BAP (control), 0.1 ppm NAA without BAP, NAA + BAP 0.5 ppm 0, while the leaf explants is there a 0.1 ppm NAA without BAP, NAA 0.1 ppm BAP + 0.5 ppm, 0.5 ppm BAP without NAA and NAA + BAP 0:01 ppm 1 ppm. Highest number of shoots obtained at a concentration of 0 ppm NAA concentration and NAA and 0.01 for leaf explants at a concentration of 0:01 ppm NAA + BAP 1 ppm and 0 ppm NAA + BAP 0.5 ppm for stem explants. Highest number of roots was found in 0:01 ppm for NAA concentration and leaf explants at a concentration of 0.1 ppm NAA + BAP and NAA 00:01 0 ppm ppm + 0.5 ppm BAP stem explants. Results of statistical analysis showed that there were significant differences of NAA and BAP concentrations on plant propagation patchouli (Pogostemon cablin Benth). Keywords: tissue culture, NAA, BAP, plant nilam (Pogostemon cablin Benth)

scholarly journals Mass Production of Bananas and Plantains (Musa spp.) Plantlets through in vitro Tissue Culture Partway: A Review

2021 ◽  
Vol 2 (4) ◽  
pp. 1-8
Author(s):  
Eustache T. A. E. Agbadje ◽  
Arnaud Agbidinoukoun ◽  
Martine Zandjanakou-Tachin ◽  
Gilles T. H. Cacaï ◽  
Corneille Ahanhanzo
Keyword(s):  
Tissue Culture ◽  
De Novo ◽  
Control Measure ◽  
Shoot Proliferation ◽  
Control Measures ◽  
Direct Organogenesis ◽  
Musa Spp ◽  
In Vitro Tissue Culture ◽  
Planting Material

Bananas and plantains are among the most important food crops in Central and West Africa. Their plantation is lead to many problems. In the recent decades, biotechnology tools using in vitro culture technics are used for the mass and free disease plantlets production in order to increase the bananas production and the yield. The main way of in vitro tissue culture at this end is the direct organogenesis i.e., the ability of plant tissues to form various organs de novo by shoots or roots induction to differentiate from a cell or cell clusters. This review aims to summarize the main results obtained in the organogenesis of bananas and plantains (Musa spp.) under in vitro conditions and to identify the challenges during the process. The research articles used in this review show that micropropagation is a reliable alternative to conventional production system of bananas and plantains planting material. However, the use of the in vitro micropropagation for bananas and plantains entails choosing the optimal explant type and size according to objectives. Benzylaminopurine remains the preferred cytokinin for in vitro banana and plantain shoot proliferation, while the use of thidiazuron appears to be more and more common. Whichever cytokinin used, the optimal cytokinin concentration for shoot proliferation is genotype dependent. This review also focuses on the causes and control measures of the two major banana and plantain micropropagation constraints: lethal tissues browning/darkening and microbial contaminations. It showed that applying the suitable and available control measure, according to the evolution of culture, is necessary. All this available information on the in vitro conditions makes banana and plantain cultivars in vitro organogenesis possible.

scholarly journals In vitro morphogenesis of Physalis ixocarpa Brot ex. Horm

2021 ◽  
Vol 51 ◽  
Author(s):  
Domitzel Zagal Alvarado ◽  
Andressa Priscila Piancó Santos Lima ◽  
José Raniere Ferreira de Santana ◽  
Alone Lima-Brito
Keyword(s):  
Tissue Culture ◽  
Experimental Design ◽  
Cotyledonary Node ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Indirect Organogenesis ◽  
In Vitro Morphogenesis ◽  
Important Species ◽  
Randomized Experimental Design

ABSTRACT Physalis ixocarpa Brot. ex Horm. is considered the most economically important species of the genus. Tissue culture is pointed out as a strategy for its propagation, but researches indicate that in vitro responses are genotype-dependent. This study aimed to evaluate the in vitro morphogenesis of the P. ixocarpa green and purple varieties, in view of the massive propagation of the species. The morphogenic capacity of the explants cotyledonary node, hypocotyl and cotyledon was evaluated in Murashige & Skoog medium supplemented with benzylaminopurine - BAP (0.00, 2.5, 5.0, 7.5 or 10.0 μM) and naphthaleneacetic acid - NAA (0.00 or 2.5 μM), using a completely randomized experimental design, in a 3 x 5 x 2 factorial scheme, with 30 treatments for each variety. The number of shoots per direct and indirect organogenesis and the percentage of explants with callus were analyzed. The in vitro morphogenetic expression of P. ixocarpa is influenced by the type of explant and by the plant regulators BAP and NAA. The cotyledonary node explant is efficient for the production of shoots via direct organogenesis in the two varieties studied.

REVIEW: In vitro plant regeneration studies and their potential applications in Populus spp.: a review

2016 ◽  
Vol 63 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Ayesh Gaur ◽  
Pankaj Kumar ◽  
Ajay Kumar Thakur ◽  
Dinesh Kumar Srivastava
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Large Scale ◽  
Direct Organogenesis ◽  
Attractive Alternative ◽  
Special Role ◽  
Scale Production ◽  
Multiplication Rate ◽  
Indirect Organogenesis

Genus Populus comprises about 25–35 species of deciduous flowering plants in the family Salicaceae which are widely distributed in temperate climates of the Northern Hemisphere. Populus species are important resources in certain branches of industry and have a special role for the scientific study of biological and agricultural systems. The poplar is known for its remarkable significance among the commercially propagated tree species such as teak, eucalyptus, wild cherry, red wood, and radiata pine. In vitro regeneration refers to growing and multiplications of cells, tissues and organs on defined liquid/solid media under aseptic and controlled environments. In vitro clonal propagation of forest trees, due to the high multiplication rate, is an attractive alternative for rapid propagation of elite genotypes of those species that could not easily be propagated through conventional methods. Owing to their widespread uses at the industrial level and for meeting the ever-increasing global demand for biomass production and wood industry, tissue culture techniques can be exploited for rapid cloning and large-scale production of planting material of various poplar species. Recent progress in the field of plant tissue culture determined this area to be one of the most dynamic and promising for experimental biology. Much work has been carried out on in vitro plant regeneration studies in Populus spp. including direct organogenesis, indirect organogenesis and somatic embryogenesis. These reviews provide an insight for in vitro plant regeneration studies in poplar species and their potential in its improvement.

RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Jyothi R ◽  
Srinivasa Murthy K M ◽  
Hossein . ◽  
Veena .
Keyword(s):  
Tissue Culture ◽  
Wide Distribution ◽  
Colocasia Esculenta ◽  
Limiting Factor ◽  
Promising Alternative ◽  
Rapid Multiplication ◽  
Medical Plant ◽  
Total Fat ◽  
Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of  tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

1959 ◽  
Vol XXXII (I) ◽  
pp. 41-53 ◽  
Author(s):  
Stig Kullander ◽  
Bengt Källén
Keyword(s):  
Tissue Culture ◽  
Granulosa Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

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