Effect of Nitrate Content of Forage on the Production of Volatile Fatty Acids by Sheep Rumen Microbes in in vitro

Rumen microbes contain a high proportion (20 to 50%) of their fatty acids (FA) as odd and branched chain fatty acids (OBCFA; C15:0, iso C15:0, anteiso C15:0, C17:0; iso C17:0; anteiso C17:0 and C17:1) and different bacterial classes have distinctive OBCFA ‘fingerprints’. As OBCFA make up around 5% of FA in milk, it has been suggested that there is scope for these compounds to be used in on-farm diagnostic milk-based tests in relation to the rumen fermentation pattern. Correlations of milk OBCFA with rumen fermentation pattern were recently shown (Vlaeminck et al., 2002). In the current in vitro study, the potential of rumen OBCFA to predict the production of volatile fatty acids (VFA) was evaluated.

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Proteksi Protein Ampas Tahu dengan Crude Palm Oil (CPO) terhadap Degradasi Mikroba Rumen

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v7i2.11459 ◽

2020 ◽

Vol 7 (2) ◽

pp. 147

Author(s):

Nadia Ainunisa ◽

Mohamad Busaeri Rapsanjani ◽

Ana Rohana Tarmidi ◽

Iman Hernaman

Keyword(s):

Fatty Acids ◽

Organic Matter ◽

Palm Oil ◽

Microbial Degradation ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Crude Palm Oil ◽

Rumen Microbes ◽

Vitro Protein

ABSTRAKProtein ampas tahu memiliki kualitas yang baik, namun mudah didegradasi oleh mikroba rumen, sehingga membutuhkan perlindungan. Penelitian bertujuan untuk melindungi protein ampas tahu dari degradasi mikroba rumen. Penelitian dilaksanakan secara eksperimental dengan menggunakan rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan. Data yang terkumpul dilakukan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Ampas tahu kering dicampur dengan menggunakan mikser secara merata dengan crude palm oil (CPO) sebanyak 0%, 10%, 20%, dan 30 % atau setara dengan 0% (P0), 4% (P1), 8% (P2), 12% (P3) dalam ransum. Kemudian ampas tahu tersebut digunakan sebagai konsentrat tunggal dalam ransum yang dicampur dengan rumput pada perbandingan 40:60, setelah itu dievaluasi secara in vitro. Hasil menunjukkan bahwa proteksi ampas tahu dengan CPO menghasilkan penurunan konsentrasi N-NH3 (P<0,05) dengan nilai yang terendah adalah 2,6 mM pada P3. Sementara itu proteksi CPO juga menurunkan nilai asam lemak terbang pada P3 dan semakin tinggi penggunaan CPO menghasilkan kecernaan bahan kering dan kecernaan bahan organik yang rendah (P<0,05). Kesimpulan, penggunaan CPO mampu memproteksi protein ampas tahu, namun dapat menurunkan asam lemak terbang dan kecernaan.Kata kunci: ampas tahu, crude palm oil (CPO), in vitro, protein, ruminansia ABSTRACT Tofu cake protein has good quality but is easily degraded by rumen microbes, so it needs protection. The research aims to protect the tofu cake protein from rumen microbial degradation. The study was conducted experimentally using a randomized complete design with 4 treatments and 5 replications. The data collected was analyzed for variance analysis and followed by Duncan's test. Dried tofu waste is mixed by using mixer evenly with crude palm oil (CPO) as much as 0%, 10%, 20%, and 30% or equivalent to 0% (P0), 4% (P1), 8% (P2), 12% (P3) in the ration. Then that tofu cake was used as a single concentrate in the ration mixed with grass at a ratio of 40:60, after which it was evaluated in vitro. The results showed that the protection of tofu cake with CPO resulted in a decrease in N-NH3 concentration (P<0.05) with the lowest value being 2.6 mM at P3. Meanwhile, CPO protection also reduced the value of volatile fatty acids at P3 and the higher use of CPO resulted in low dry matter and organic matter digestibility (P<0.05). In conclusion, the use of CPO is able to protect the protein of tofu cake, but it can reduce volatile fatty acids and digestibility.Keywords: crude palm oil (CPO), in vitro, protein, ruminant, tofu cake

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Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽

10.3390/ani11010108 ◽

2021 ◽

Vol 11 (1) ◽

pp. 108

Author(s):

Yichong Wang ◽

Sijiong Yu ◽

Yang Li ◽

Shuang Zhang ◽

Xiaolong Qi ◽

...

Keyword(s):

Fatty Acids ◽

Acetic Acid ◽

Propionic Acid ◽

Acid Content ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

Gas Production ◽

Quadratic Response ◽

In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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Effects of Increasing Doses of Condensed Tannins Extract from Cistus ladanifer L. on In Vitro Ruminal Fermentation and Biohydrogenation

Animals ◽

10.3390/ani11030761 ◽

2021 ◽

Vol 11 (3) ◽

pp. 761

Author(s):

Olinda Guerreiro ◽

Susana P. Alves ◽

Mónica Costa ◽

Maria F. Duarte ◽

Eliana Jerónimo ◽

...

Keyword(s):

Fatty Acids ◽

Condensed Tannins ◽

Volatile Fatty Acids ◽

Secondary Compounds ◽

Cistus Ladanifer ◽

Ruminal Fermentation ◽

Experimental Conditions ◽

Ct Dose ◽

Linear Decrease

Cistus ladanifer (rockrose) is a perennial shrub quite abundant in the Mediterranean region, and it is a rich source in secondary compounds such as condensed tannins (CTs). Condensed tannins from C. ladanifer were able to change the ruminal biohydrogenation (BH), increasing the t11–18:1 and c9,t11–18:2 production. However, the adequate conditions of the C. ladanifer CTs used to optimize the production of t11–18:1 and c9,t11–18:2 is not yet known. Thus, we tested the effect of increasing the doses of C. ladanifer CT extract (0, 25, 50, 75 and 100 g/kg dry matter (DM)) on in vitro rumen BH. Five in vitro batch incubations replicates were conducted using an oil supplemented high-concentrate substrate, incubated for 24 h with 6 mL of buffered ruminal fluid. Volatile fatty acids (VFAs) and long chain fatty acids (FA) were analyzed at 0 h and 24 h, and BH of c9–18:1, c9, c12–18:2 and c9, c12, c15–18:3, and BH products yield were computed. Increasing doses of C. ladanifer CTs led to a moderate linear decrease (p < 0.001) of the VFA production (a reduction of 27% with the highest dose compared to control). The disappearance of c9–18:1 and c9,c12–18:2 as well as the production of t11–18:1 and c9, t11:18:2 was not affected by increasing doses of C. ladanifer CTs, and only the disappearance of c9, c12, c15–18:3 suffered a mild linear decrease (a reduction of 24% with the highest dose compared to control). Nevertheless, increasing the C. ladanifer CT dose led to a strong depression of microbial odd and branched fatty acids and of dimethyl acetals production (less than 65% with the highest dose compared to control), which indicates that microbial growth was more inhibited than fermentative and biohydrogenation activities, in a possible adaptative response of microbial population to stress induced to CTs and polyunsaturated fatty acids. The ability of C. ladanifer to modulate the ruminal BH was not verified in the current in vitro experimental conditions, emphasizing the inconsistent BH response to CTs and highlighting the need to continue seeking the optimal conditions for using CTs to improve the fatty acid profile of ruminant fat.

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Absorption and metabolism of volatile fatty acids by rumen and omasum

Ciência e Agrotecnologia ◽

10.1590/s1413-70542012000100012 ◽

2012 ◽

Vol 36 (1) ◽

pp. 93-99 ◽

Cited By ~ 6

Author(s):

João Luiz Pratti Daniel ◽

João Chrysostomo de Resende Júnior

Keyword(s):

Fatty Acids ◽

Mitotic Index ◽

Volatile Fatty Acids ◽

Diffusion Chamber ◽

Serosal Side ◽

Buffer Solution ◽

Mucosal Side ◽

Over Time ◽

Fractional Absorption

Volatile fatty acids (VFA) absorption and metabolic capacity of rumen and omasum were compared, in vitro. Fragments of rumen wall and omasum laminae were taken from eight adult crossbred bovines. An isolated fragment of the mucosa was fitted in a tissue diffusion chamber. Valeric acid and CrEDTA were added to ruminal fluid and placed on the mucosal side and buffer solution was placed on the serosal side. Fractional absorption rates were measured by exponential VFA:Cr ratio decay over time. Metabolism rate was determined as the difference between VFA absorbed and VFA which appeared on the serosal side over time. Mitotic index was higher in omasum (0.52%) than in rumen epithelium (0.28%). VFA fractional absorption rate was higher in omasum (4.6%/h.cm²) than in rumen (0.4%/h.cm²). Acetate, propionate, butyrate, and valerate showed similar fractional absorption rates in both fragments. Percentage of metabolized acetate and propionate was lower than butyrate and valerate in both stomach compartments. In the rumen, individual VFA metabolism rates were similar (mean of 7.7 , but in the omasum, valerate (90.0 was more metabolized than butyrate (59.6 propionate (69.8 and acetate (51.7 . Correlation between VFA metabolism and mitotic index was positive in the rumen and in the omasum. In conclusion, VFA metabolism and absorption potential per surface of the omasum is higher than that of the rumen. Variations on rumen and omasum absorption capacities occur in the same way, and there are indications that factors capable of stimulating rumen wall proliferation are similarly capable of stimulating omasum walls.

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Variability of in vitro ruminal fermentation and methanogenic potential in the pasture legume biserrula (Biserrula pelecinus L.)

Crop and Pasture Science ◽

10.1071/cp13073 ◽

2013 ◽

Vol 64 (4) ◽

pp. 409 ◽

Cited By ~ 7

Author(s):

Bidhyut Kumar Banik ◽

Zoey Durmic ◽

William Erskine ◽

Phillip Nichols ◽

Kioumars Ghamkhar ◽

...

Keyword(s):

Core Collection ◽

Volatile Fatty Acids ◽

Germplasm Collection ◽

Red Clover ◽

Subterranean Clover ◽

Ruminal Fermentation ◽

Rumen Microbes ◽

Pasture Legumes ◽

Pasture Legume

Biserrula (Biserrula pelecinus L.) is an important annual pasture legume for the wheatbelt of southern Australia and has been found to have lower levels of methane output than other pasture legumes when fermented by rumen microbes. Thirty accessions of the biserrula core germplasm collection were grown in the glasshouse to examine intra-specific variability in in vitro rumen fermentation, including methane output. One biserrula cultivar (Casbah) was also grown at two field locations to confirm that low methanogenic potential was present in field-grown samples. All of the biserrula accessions had significantly reduced methane [range 0.5–8.4 mL/g dry matter (DM)] output compared with subterranean clover (28.4 mL/g DM) and red clover (36.1 mL/g DM). There was also significant variation in fermentability profiles (except for volatile fatty acids) among accessions of the core collection. Methanogenic potential exhibited 86% broad-sense heritability within the biserrula core collection. The anti-methanogenic and gas-suppressing effect of biserrula was also confirmed in samples grown in the field. In conclusion, biserrula showed variability in in vitro fermentation traits including reduced methane production compared with controls. This bioactivity of biserrula also persists in the field, indicating scope for further selection of biserrula as an elite methane-mitigating pasture.

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Chemical Additives in Rumen Fermentations: In Vitro Effects of Various Drugs on Rumen Volatile Fatty Acids and Protozoa

Journal of Animal Science ◽

10.2527/jas1970.305812x ◽

1970 ◽

Vol 30 (5) ◽

pp. 812-818 ◽

Cited By ~ 9

Author(s):

J. J. O'Connor ◽

G. S. Myers ◽

D. C. Maplesden ◽

G. W. Vander Noot

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Chemical Additives ◽

In Vitro Effects

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Profiles of Odd- and Branched-Chain Fatty Acids and Their Correlations With Rumen Fermentation Parameters, Microbial Protein Synthesis and Bacterial Populations Based on Pure Carbohydrate Incubation in Vitro

10.21203/rs.3.rs-68439/v1 ◽

2020 ◽

Author(s):

Hangshu Xin ◽

Xin Liu ◽

Xin Jiang ◽

Chunlong Liu ◽

Shuzhi Zhang ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Cellulolytic Bacteria ◽

Bacterial Populations ◽

Branched Chain Fatty Acids ◽

Degrading Bacteria ◽

Branched Chain ◽

Fermentation Parameters ◽

Chain Fatty Acids

Abstract Background: The objectives of this study were to evaluate the profiles of odd- and branched-chain fatty acids (OBCFA; including C15:0, iso-C15:0, anteiso-C15:0, iso-C16:0, C17:0, iso-C17:0 and anteiso-C17:0) during pure carbohydrates incubation in vitro and whether they correlated with ruminal fermentation parameters, microbial crude protein (MCP) synthesis, and bacterial populations. The pure substrates containing five different ratios of fiber and starch (F:S; 0:100, 25:75, 50:50, 75:25 and 100:0) were incubated for 6 h, 12 h, 18 h and 24 h. Results: Except iso-C17:0, OBCFA concentrations were interacted by F:S and incubation time. The highest concentration of total OBCFA was found in the fermented mixture after 24 h of incubation when the F:S = 0:100; while the lowest level was 1.65 mg/g DM produced after 6 h of incubation with F:S = 50:50. The concentrations of total volatile fatty acids (TVFA) and MCP remarkably decreased linearly as the inclusion of fiber in the substrates increased, as expected. The proportions of investigated cellulolytic bacteria in our study were increased linearly (or linearly and quadratically) while those of R. amylophilus and S. bovis were decreased as fiber inclusion increased. The correlation analysis indicated that iso-C16:0 concentration might have potential as a marker of productions of TVFA and MCP with ρ being 0.78 and 0.82 respectively. Compared to starch degrading bacteria, cellulolytic bacteria had more correlations with OBCFA profiles, and the strongest association was found on the population of R. flavefaciens with C15:0 concentration (ρ = 0.70). Conclusions: Our study shows there might be scope for iso-C16:0 to predict rumen productions of VFA and MCP. Notedly, this is the first paper reporting linkage of OBCFA with rumen function based on pure carbohydrate in vitro incubation, which would avoid confounding interference from dietary protein and fat presence. However, more in-depth experiments are needed to substantiate the current findings.

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