scholarly journals Cancer selective cytotoxicity of Sida acuta extracts on Artemia salina and human breast adenocarcinoma cells

2021 ◽  
pp. 4-9
Author(s):  
Ramasamy Elankanni ◽  
Devanga Ragupathi Naveen Kumar ◽  
Rangasamy Ashok Kumar
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Therapeutic Potential ◽  
Artemia Salina ◽  
Breast Adenocarcinoma ◽  
Apoptotic Cells ◽  
Human Breast ◽  
Sida Acuta ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Cancer is one of the major causes of morbidity and mortality globally. The interests in the use of plants or plant-derived compounds are increasing recently due to their promising results in chemoprevention. The present study investigates the anti-cancer potentials of Sida acuta, a traditionally well-known medicinal plant. Accordingly, the methanol and aqueous extracts of S. acuta (SAM and SAA) were tested against Artemia salina nauplii for toxicity and on MDA-MB-231 and MCF-7 human breast adenocarcinoma cell lines for cytotoxic and apoptotic properties. Both the extracts, SAM and SAA exhibited higher toxicity towards Artemia salina. Interestingly, the extracts exhibited minimal cytotoxicity in normal cells (VERO) than in human breast cancer cells (MDA-MB-231 and MCF-7). The highly active SAA successfully induced apoptosis in MDA MB 231 and MCF-7 cells showing 17.81% and 4.27% of late apoptotic cells and 27.14% and 37.32% of early apoptotic cells, respectively. Most of the drugs being developed from plant sources had landed successfully in clinical trials. In conclusion, the observations clearly suggest that SAA may have possible therapeutic potential against human breast cancer-derived diseases specifically against ER-positive breast cancer.

scholarly journals Annihilating efficacy of Euphorbia hirta L. extracts on Artemia salina Nauplii and human breast adenocarcinoma cells (MCF-7 & MDA-MB-231)

2021 ◽  
pp. 91-94
Author(s):  
Ramasamy Elankanni ◽  
Devanga Ragupathi Naveen Kumar ◽  
Rangasamy Ashok Kumar
Keyword(s):  
Breast Cancer ◽  
Therapeutic Potential ◽  
Artemia Salina ◽  
Breast Adenocarcinoma ◽  
Breast Cancers ◽  
Human Breast ◽  
Anticancer Activities ◽  
Euphorbia Hirta ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Cancer is one of the major causes of death both in developed and developing countries. Recently the secondary metabolites produced by plants are being investigated due to their promising anticancer activities. Accordingly in the present study the anti-cancer potentials of Euphorbia hirta L., a well-known medicinal plant was explored for its anticancer activity. The methanol and aqueous extracts of Euphorbia hirta L. (EHA and EHM) were tested against Artemia salina nauplii for toxicity and MDA-MB-231 and MCF-7 human breast adenocarcinoma cell lines for its cytotoxic potentials. Both the extracts EHA and EHM exhibited maximum toxicity towards Artemia salina among which the methanol extract was able to kill all the nauplii in its highest concentration. Excitingly, Euphorbia hirta L. extracts exhibited minimal cytotoxicity on normal cells (VERO) than in human breast cancer cells (MDA-MB-231 and MCF-7). In conclusion, the results suggest that EHM extract of the selected plant may have promising therapeutic potential against human breast cancers and may lead to the development of new clinical drug specifically against ER-positive breast cancer.

scholarly journals Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2634
Author(s):  
Farzana Nazir ◽  
Mudassir Iqbal
Keyword(s):  
Breast Cancer ◽  
Mouse Skin ◽  
Breast Adenocarcinoma ◽  
Cellulose Derivatives ◽  
Skin Melanoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Cytotoxicity Studies ◽  
Melanoma B16f10 ◽  
Mcf 7

Cellulose based materials are emerging in the commercial fields and high-end applications, especially in biomedicines. Aminated cellulose derivatives have been extensively used for various applications but limited data are available regarding its cytotoxicity studies for biomedical application. The aim of this study is to synthesize different 6-deoxy-amino-cellulose derivatives from Microcrystalline cellulose (MCC) via tosylation and explore their cytotoxic potential against normal fibroblasts, melanoma and breast cancer. 6-deoxy-6-hydrazide Cellulose (Cell Hyd) 6-deoxy-6-diethylamide Cellulose (Cell DEA) and 6-deoxy-6-diethyltriamine Cellulose (Cell DETA) were prepared and characterized by various technologies like Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR), nuclear magnetic resonance spectroscopy (NMR), X-ray diffractogram (XRD), Scanning Electron microscopy (SEM), Elemental Analysis and Zeta potential measurements. Cytotoxicity was evaluated against normal fibroblasts (NIH3T3), mouse skin melanoma (B16F10), human epithelial adenocarcinoma (MDA-MB-231) and human breast adenocarcinoma (MCF-7) cell lines. IC50 values obtained from cytotoxicity assay and live/dead assay images analysis showed MCC was non cytotoxic while Cell Hyd, Cell DEA and Cell DETA exhibited noncytotoxic activity up to 200 μg/mL to normal fibroblast cells NIH3T3, suggesting its safe use in medical fields. The mouse skin melanoma (B16F10) are the most sensitive cells to the cytotoxic effects of Cell Hyd, Cell DEA and Cell DETA, followed by human breast adenocarcinoma (MCF-7). Based on our study, it is suggested that aminated cellulose derivatives could be promising candidates for tissue engineering applications and in cancer inhibiting studies in future.

Anti-cancer Effects of Epigenetics Drugs Scriptaid and Zebularine in Human Breast Adenocarcinoma Cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Zhi Hung Yap ◽  
Wei Yang Kong ◽  
Abdur Rahmaan Azeez ◽  
Chee-Mun Fang ◽  
Siew Ching Ngai
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Migration ◽  
Histochemical Staining ◽  
Breast Adenocarcinoma ◽  
Nuclear Chromatin ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Anti Cancer ◽  
Mcf 7

Background: High relapse and metastasis progression in breast cancer patients have prompted the need to explore alternative treatments. Epigenetic therapy has emerged as an attractive therapeutic strategy due to the reversibility of epigenome structures. Objective: This study investigated the anti-cancer effects of epigenetic drugs scriptaid and zebularine in human breast adenocarcinoma MDA-MB-231 and MCF-7 cells. Methods: First, the half maximal inhibitory concentration (IC50) of scriptaid, zebularine and the combination of both drugs on human breast adenocarcinoma MDA-MB-231 cells was determined. Next, MDA-MB-231 and MCF-7 cells were treated with scriptaid, zebularine and the combination of both. After treatments, the anti-cancer effects were evaluated via cell migration assay, cell cycle analysis and apoptotic studies, which included histochemical staining and reverse-transcriptase polymerase chain reaction (RT-PCR) of the apoptotic genes. Results: Both epigenetic drugs inhibited cell viability in a dose-dependent manner with 2 nM scriptaid, 8 µM zebularine and combination of 2 nM scriptaid and 2 µM zebularine. Both MDA-MB-231 and MCF-7 cells exhibited a reduction in cell migration after the treatments. In particular, MDA-MB-231 cells exhibited a significant reduction in cell migration (p < 0.05) after the treatments of zebularine and the combination of scriptaid and zebularine. Besides, cell cycle analysis demonstrated that scriptaid and the combination of both drugs could induce cell cycle arrest at the G0/G1 phase in both MDA-MB-231 and MCF-7 cells. Furthermore, histochemical staining allowed the observation of apoptotic features, such as nuclear chromatin condensation, cell shrinkage, membrane blebbing, nuclear chromatin fragmentation and cytoplasmic extension, in both MDA-MB-231 and MCF-7 cells after the treatments. Further apoptotic studies revealed that the upregulation of pro-apoptotic Bax, downregulation of anti-apoptotic Bcl-2 and elevation of Bax/Bcl-2 ratio were found in MDA-MB-231 cells treated with zebularine and MCF-7 cells treated with all drug regimens. Conclusion: Collectively, these findings suggest that scriptaid and zebularine are potential anti-cancer drugs, either single or in combination, for the therapy of breast cancer. Further investigations of the gene regulatory pathways directed by scriptaid and zebularine are definitely warranted in the future.

scholarly journals Analysis and Identification of Active Compounds from Gami-Soyosan Toxic to MCF-7 Human Breast Adenocarcinoma Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 272 ◽  
Author(s):  
Mi-Yeon Jung ◽  
Chang-Seob Seo ◽  
Seon-Eun Baek ◽  
Jaemin Lee ◽  
Myoung-Sook Shin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gallic Acid ◽  
Parp Cleavage ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Decursinol Angelate ◽  
Adenocarcinoma Cells ◽  
Er Positive ◽  
Mcf 7

Gami-soyosan is a medicinal herbal formulation prescribed for the treatment of menopausal symptoms, including hot flashes and osteoporosis. Gami-soyosan is also used to treat similar symptoms experienced by patients with breast cancer. The incidence of breast cancer in women receiving hormone replacement therapy is a big burden. However, little is known about the components and their mechanism of action that exhibit these beneficial effects of Gami-soyosan. The aim of this study was to simultaneously analyze compounds of Gami-soyosan, and determine their cytotoxic effects on estrogen receptor (ER)-positive MCF-7 human breast adenocarcinoma cells. We established a simultaneous analysis method of 18 compounds contained in Gami-soyosan and found that, among the various compounds in Gami-soyosan, gallic acid (1), decursin (17), and decursinol angelate (18) suppressed the viability of MCF-7 cells. Gallic acid (1), decursin (17), and decursinol angelate (18) induced apoptotic cell death and significantly increased poly (ADP-ribose) polymerase (PARP) cleavage and the Bcl-2-associated X protein/ B-cell lymphoma 2 (Bax/Bcl-2) ratio. Decursin (17) increased the expression of cleaved caspases-8, -9, -7, and -3. Decursinol angelate (18) increased the expression of cleaved caspase-8 and -7. These three components altered the different apoptosis signal pathways. Collectively, gallic acid (1), decursin (17), and decursinol angelate (18) may be used to inhibit cell proliferation synergistically in patients with ER-positive breast cancer.

scholarly journals Calophyllum brasiliense Extracts Induced Apoptosis in Human Breast Adenocarcinoma Cells

2021 ◽  
pp. 50-64
Author(s):  
Michelle S. F. Correia ◽  
Anuska M. Alvares-Saraiva ◽  
Elizabeth C. P. Hurtado ◽  
Mateus L. B. Paciencia ◽  
Fabiana T. C. Konno ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Breast Cancer Cell ◽  
Breast Adenocarcinoma ◽  
Apoptosis Induction ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Calophyllum Brasiliense ◽  
Apoptosis And Necrosis ◽  
Mcf 7

Aims: Apoptosis, or programmed cell death, is linked to several mechanisms of cell growth control. The present work aimed at evaluating the induction of apoptosis in MCF-7 human breast adenocarcinoma cell by Calophyllum brasiliense. Study design: The tests were performed in triplicates in the apoptosis assays and sextuplicates in the cytotoxic assays, to each group, and the data expressed the mean +/- standard deviations. The cytotoxicity IC50s were obtained based on nonlinear regression curve fit. Two-way ANOVA and Tukey’s tests were applied in the apoptosis analyses. Place and duration of study: The work was done at the Center for Research in Biodiversity (Cell Culture Laboratory, and Phytochemistry Laboratory), and Research Center (Molecular Biology Laboratory), Paulista University, between Jan 2019 and Dec 2019. Methodology: Two aqueous extracts, obtained from the stem (STE) and from the leaves (LFE) of Calophyllum brasiliense by a 24-h maceration, were submitted to a cytotoxic assay against MCF-7 breast cancer cell lines at the concentrations of 0.01 µg/ml, 0.1 µg/ml, 1.0 µg/ml, 10 µg/ml and 100 µg/ml. They were also subjected to the evaluation of apoptosis and necrosis cell death induction at concentrations of 50, 100 and 200 μg/ml, after 6 h, 12 h and 24 h. Curcumin was used as a reference drug for both cytotoxic (50 mM, 5.0 mM, 0.5 mM, 0.05 mM and 0.005 mM ) and apoptosis/necrosis (12.5 μM, 25 μM and 50 μM / 6 h, 12 h and 24 h) assays. Apoptosis and necrosis were accessed by the use of annexin V and 7-AAD, in a two-channel flow cytometer. Results: In terms of the cytotoxic activity, STE (IC50 7.86 µg/ml) was more toxic than LFE (IC50 74.35 µg/ml), and curcumin IC50 was 0.00159 µg/ml. STE induced 21.19 % and LFE, 20.63 %, in comparison to 13.4% of apoptosis induction by curcumin. The results of apoptosis induction in the cancer cells were achieved at 24 h, extract concentrations at 100 µg/ml. Conclusion: Both the extracts, STE and LFE, were cytotoxic against MCF-7 breast cancer cell line, and induced more apoptosis in MCF-7 cells than curcumin, suggesting that they are high potential sources of natural product-inducing apoptosis agents to be used against adenocarcinoma breast cells.

scholarly journals Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells

2020 ◽  
Vol 23 (7) ◽  
pp. 234-237
Author(s):  
Dudi Tohir ◽  
Fitriah Sari
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Human Breast Cancer ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Active Fraction ◽  
Human Breast ◽  
Swietenia Macrophylla ◽  
Brine Shrimp Lethality Assay ◽  
Mcf 7

Ethyl acetate fraction from Swietenia macrophylla was reported to have toxicity against the larvae of Artemia salina shrimp larvae. However, there areno reports aboutS. macrophylla, which can inhibit human breast cancer cells MCF-7. Therefore, this study aims to evaluate S. macrophyllaextract's cytotoxicity using human breast cancer MCF-7 cells assay, followed by confirmation of its toxicity using brine shrimp lethality assay. The most active fraction obtained from the ethyl acetate extract of S. macrophylla showed 76.49% inhibition at 50 µg/mL (IC50=34.11 µg/mL). At the same time, the most active fraction may contain a mixture of limonoid compounds after LCMS analysis. The most active fraction obtained from ethyl acetate extract of S. macrophylla showed 76.49%

DNA Adduct Formation by the Anticancer Drug Ellipticine and Its Hydroxy Derivatives in Human Breast Adenocarcinoma MCF-7 Cells

2004 ◽  
Vol 69 (3) ◽  
pp. 603-615 ◽  
Author(s):  
Lucie Bořek-Dohalská ◽  
Eva Frei ◽  
Marie Stiborová
Keyword(s):  
Breast Cancer ◽  
Cytochrome P450 ◽  
Cancer Cells ◽  
Dna Adducts ◽  
Breast Adenocarcinoma ◽  
Antitumor Action ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

The cytotoxicity of the antineoplastic agent ellipticine and its 9- and 7-hydroxylated metabolites to human breast adenocarcinoma MCF-7 cells and their ability to generate DNA adducts in these cancer cells were investigated. Ellipticine and its 9-hydroxylated metabolite were found to be toxic to MCF-7 cells with IC50 values of 1.25 and 3.25 μmol l-1 for ellipticine and 9-hydroxyellipticine, respectively. In contrast, no toxicity to these cancer cells was detectable for 7-hydroxyellipticine. The nuclease P1 version of the 32P-postlabeling assay yielded a pattern of ellipticine-DNA adducts with two major and one minor adducts in MCF-7 cells, similar to the pattern of adducts detected in DNA reacted with ellipticine and the reconstituted cytochrome P450 enzyme system in vitro and in DNA in vivo. The identity of two major adducts formed in DNA of MCF-7 cells with those formed by cytochrome P450-mediated ellipticine activation in vitro was confirmed by HPLC of the isolated adducts. 9-Hydroxyellipticine was also capable of inducing DNA adducts in MCF-7 cells, but to a lesser extent. In addition, the adducts generated by 9-hydroxyellipticine were different from those generated by ellipticine. Negligible levels of DNA adducts were detectable in DNA of MCF-7 cells exposed to 7-hydroxyellipticine. The results presented here are the first report showing the formation of covalent DNA adducts with ellipticine in human breast cancer cells in culture, and suggest the formation of covalent DNA adducts as a new mode of antitumor action of ellipticine in breast cancer.

scholarly journals The Cytotoxic Effects of Humic Acid on Human Breast Cancer Cells

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1565
Author(s):  
Asli Aykac ◽  
Eda Becer ◽  
Tuğçe Balcı Okcanoğlu ◽  
Meryem Güvenir ◽  
Kaya Süer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Humic Acid ◽  
Cell Line ◽  
Cytotoxic Effect ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Cytotoxic Effects ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Breast cancer is the most common cancer among women and in order to create alternative treatments different types of in vivo and in vitro studies have used various plant-based therapeutic agents. Humic acid (HA) induces apoptosis and has various pharmacological properties including anti-inflammatory and anti-proliferative effects. In our study, we examined the cytotoxic effects of HA at concentrations of 5, 10, 20, 50 and 100 μg/mL in human breast adenocarcinoma MCF-7 cell line for 24 and 48 h. By using MTT method, it has been found out that HA 100 g/mL had cytotoxic effect on human breast adenocarcinoma MCF-7 cell line at both 24 and 48 h; also found out that the effective dose of HA at the same time (24 and 48 h) was 50 μg/mL. The results of our study will shed light on the development of alternative therapeutic approaches in the treatment of cancer by evaluating the cytotoxic effect of HA.

scholarly journals FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 cells): Morphometric Analysis

2018 ◽  
Author(s):  
Firdos Alam Khan ◽  
Sultan Akhtar ◽  
Sarah Ameen Almofty ◽  
Dana Almohazey ◽  
Munthar Alomari
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Breast Adenocarcinoma ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Surgical Interventions ◽  
Mcf 7

Breast cancer treatment mostly revolved around radiation therapy and surgical interventions, these treatments doesn&rsquo;t provide satisfactory relief to the patients and carry unmanageable side-effects. Nanomaterials show promising results in treating cancer cells and have many advantages such as high biocompatibility, bioavailability and effective therapeutic capabilities. Interestingly, fluorescent magnetic nanoparticles have been used in many biological and diagnostic applications, but there is no report of use of fluorescent magnetic submicronic polymer nanoparticles (FMSP-nanoparticles) in the treatment of human breast cancer cells. In the present study, we have tested the effect FMSP-nanoparticles on human breast cancer cells (MCF-7). We have tested different concentrations (1.25&micro;g/1mL, 12.5&micro;g/mL and 50&micro;g/1mL) of FMSP-nanoparticles in MCF-7 cells and evaluated the nanoparticles response morphometrically. Our results revealed that FMSP-nanoparticles produced a concentration dependent effect on the cancer cells, dose of 1.25&micro;g/mL produced no significant effect on the cancer cell morphology and cell death, whereas dosages of 12.5&micro;g/mL and 50&micro;g/mL respectively showed significant nuclear augmentation, disintegration, chromatic condensation followed by dose dependent cell death. Our results demonstrate FMSP-nanoparticles have ability to induce cell death in MCF-7 cells and may be considered as a potential anti-cancer agent for breast cancer treatments.

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