scholarly journals Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions

2011 ◽  
pp. 344-348
Author(s):  
José Henry Osorio ◽  
Morteza Pourfarzam
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Saline Solution ◽  
Mass Spectrometry Analysis ◽  
Blood Samples ◽  
Spectrometry Analysis ◽  
Tandem Mass Spectrometry Analysis ◽  
Human Blood Samples ◽  
Saline Solutions ◽  
Long Chain

Objective: To evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution. Materials and methods: Human blood samples were centrifuged and the blood cells were washed with different saline solutions. The final pellet was resuspended in PBS for card preparation and tandem mass spectrometry analysis. Results: It was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. Significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05). Conclusion: Because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites.

scholarly journals Abundant circular RNA expression in terminal myeloid differentiation does not correlate with frequent circRNA translation, or with circRNA-mediated translation regulation

2020 ◽  
Author(s):  
Benoit P. Nicolet ◽  
Sjoert B.G. Jansen ◽  
Esther Heideveld ◽  
Willem H. Ouwehand ◽  
Emile van den Akker ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematopoietic Cells ◽  
Blood Stream ◽  
Mass Spectrometry Analysis ◽  
Translation Regulation ◽  
Circular Rnas ◽  
Hematopoietic Stem ◽  
Erythroid Precursor ◽  
Spectrometry Analysis

ABSTRACTEach day, about 1012 erythrocytes and platelets are released into the blood stream. This substantial output from hematopoietic stem cells is tightly regulated by transcription factors and epigenetic modifications. Whether and how non-coding RNAs such as circular RNAs (circRNAs) contribute to the differentiation and/or identity of hematopoietic cells is to date not well understood. We recently reported a circRNA expression map of hematopoietic cells, revealing that erythrocytes and platelets contain the highest levels and numbers of circRNAs. Here, we provide the first detailed and comprehensive analysis of circRNA expression during red blood cell and megakaryocyte differentiation. CircRNA expression significantly increased during erythroid precursor differentiation into red blood cells and in differentiating megakaryocytes, in particular upon enucleation. To determine if circRNAs can modulate hematopoietic differentiation, we compared the expression levels of circRNAs and mRNAs with that of ribosomal foot printing read. This multi-omics approach revealed that only 20 out of 748 (2.6%) circRNAs associated with translation regulation of their mRNA counterparts. Furthermore, irrespective of the thousands of identified putative open reading frames in circRNAs, deep ribosome-footprinting sequencing and mass spectrometry analysis provided little evidence for translation of endogenously expressed circRNAs in erythroblasts, megakaryocytes and platelets. In conclusion, circRNAs in platelets and red blood cells are highly abundant and alter their expression profile during differentiation, yet their contribution to regulate cellular processes remains enigmatic.

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