Genetic Diversity Among Ten Maize Genotypes Using Simple Sequence Repeat DNA Markers

Genetic diversity among ten maize genotypes (seven inbred lines and three testers) was investigated using ten simple sequence repeats (SSRs). Primers (bnlg128, bnlg1839, Umc1117, bnlg1144, and bnlg1152) generated the highest number of bands (4 bands) for inbred lines while the primer bnlg128 showing the highest number of bands (3 bands) for testers. The primer bnlg128 shows the highest effective number of alleles (ne) for inbred lines and testers. Among the studied primer bnlg1839 in inbred lines and primer bnlg128 in testers showed the maximum polymorphism information content (PIC) and the greatest diversity. Using UPGMA cluster analysis, the seven inbred lines were grouped under three clusters, while grouped the testers under two clusters. Most of the inbred lines which were derived from the same source population were grouped in the same cluster based on the SSRs DNA markers, indicating high genetic differentiation among their source populations. Results showed that the SSRs were informative in detecting genetic differences among the maize inbred lines and testers, as exhibited by the high average of Shannon’s information index (I), Nei’s expected heterozygosity (Nei’s), and PIC. The results suggest that the studied genotypes are diverse and may be utilized for further breeding programs.

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ISSR Marker Based Genetic Diversity in Morinda Spp. For Its Enhanced Collection, Conservation and Utilization

10.21203/rs.3.rs-666059/v1 ◽

2021 ◽

Author(s):

Lalit Arya ◽

Ramya Kossery Narayanan ◽

Anjali Kak ◽

Chitra Devi Pandey ◽

Manjusha Verma ◽

...

Keyword(s):

Genetic Diversity ◽

Gene Diversity ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Morinda Citrifolia ◽

Species Differentiation ◽

Information Index ◽

Upgma Cluster Analysis ◽

Simple Sequence

Abstract Morinda (Rubiaceae) is considerably recognized for its multiple uses viz. food, medicine, dyes, firewood, tools, oil, bio-sorbent etc. The molecular characterization of such an important plant would be very useful for its multifarious enhanced utilization. In the present study, 31 Morinda genotypes belonging to two different species Morinda citrifolia and Morinda tomentosa collected from different regions of India were investigated using Inter Simple Sequence Repeat (ISSR) markers. Fifteen ISSR primers generated 176 bands with an average of 11.7 bands per primer, of which (90.34%) were polymorphic. The percentage of polymorphic bands, mean Nei’s gene diversity, mean Shannon’s information index in Morinda tomentosa and Morinda citrifolia was [(69.89%, 30.68%); (0.21 ± 0.19, 0.12 ± 0.20); (0.32 ± 0.27 0.17 ± 0.28)] respectively, revealing higher polymorphism and genetic diversity in Morinda tomentosa compared to Morinda citrifolia. Structure, and UPGMA cluster analysis placed the genotypes into well-defined separate clusters belonging to two species Morinda tomentosa and Morinda citrifolia revealing the utility of ISSR markers in species differentiation. Distinct ecotypes within a particular species could also be inferred emphasizing the collection and conservation of Morinda genotypes from different regions, in order to capture the overall diversity of respective species. Further higher diversity of M. tomentosa must be advanced for its utilization in nutraceutical, nutritional and other nonfood purposes.

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Breeding potential and genetic diversity of "Híbrido do Timor" coffee evaluated by molecular markers

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332010000400003 ◽

2010 ◽

Vol 10 (4) ◽

pp. 298-304 ◽

Cited By ~ 12

Author(s):

Tesfahun Alemu Setotaw ◽

Eveline Teixeira Caixeta ◽

Guilherme Ferreira Pena ◽

Eunize Maciel Zambolim ◽

Antonio Alves Pereira ◽

...

Keyword(s):

Genetic Diversity ◽

Cluster Analysis ◽

Molecular Markers ◽

Genetic Structure ◽

Gene Diversity ◽

High Genetic Diversity ◽

Breeding Programs ◽

Information Index ◽

Upgma Cluster Analysis ◽

Potential Use

AFLP, RAPD and SSR molecular markers were used to study the genetic diversity and genetic structure of the Híbrido de Timor germplasm. The principal coordinate analysis, UPGMA cluster analysis based on genetic dissimilarity of Jaccard, Bayesian model-based cluster analysis, percentage of polymorphic loci, Shannon's information index and Nei gene diversity were employed to assess the genetic diversity. The analyses demonstrated a high genetic diversity among Híbrido de Timor accessions. UPGMA and Bayesian cluster analyses grouped the accessions into three clusters. The genetic structure of Híbrido de Timor is reported. The management of Híbrido de Timor germplasm variability and its potential use in breeding programs are discussed.

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Phenotypic and molecular characterization of a set of tropical maize inbred lines from a public breeding program in Brazil

BMC Genomics ◽

10.1186/s12864-021-08127-7 ◽

2022 ◽

Vol 23 (1) ◽

Author(s):

Sirlene Viana de Faria ◽

Leandro Tonello Zuffo ◽

Wemerson Mendonça Rezende ◽

Diego Gonçalves Caixeta ◽

Hélcio Duarte Pereira ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Inbred Lines ◽

Breeding Program ◽

Tropical Maize ◽

Maize Breeding ◽

Breeding Programs ◽

The Public ◽

Maize Inbred Lines

Abstract Background The characterization of genetic diversity and population differentiation for maize inbred lines from breeding programs is of great value in assisting breeders in maintaining and potentially increasing the rate of genetic gain. In our study, we characterized a set of 187 tropical maize inbred lines from the public breeding program of the Universidade Federal de Viçosa (UFV) in Brazil based on 18 agronomic traits and 3,083 single nucleotide polymorphisms (SNP) markers to evaluate whether this set of inbred lines represents a panel of tropical maize inbred lines for association mapping analysis and investigate the population structure and patterns of relationships among the inbred lines from UFV for better exploitation in our maize breeding program. Results Our results showed that there was large phenotypic and genotypic variation in the set of tropical maize inbred lines from the UFV maize breeding program. We also found high genetic diversity (GD = 0.34) and low pairwise kinship coefficients among the maize inbred lines (only approximately 4.00 % of the pairwise relative kinship was above 0.50) in the set of inbred lines. The LD decay distance over all ten chromosomes in the entire set of maize lines with r2 = 0.1 was 276,237 kb. Concerning the population structure, our results from the model-based STRUCTURE and principal component analysis methods distinguished the inbred lines into three subpopulations, with high consistency maintained between both results. Additionally, the clustering analysis based on phenotypic and molecular data grouped the inbred lines into 14 and 22 genetic divergence clusters, respectively. Conclusions Our results indicate that the set of tropical maize inbred lines from UFV maize breeding programs can comprise a panel of tropical maize inbred lines suitable for a genome-wide association study to dissect the variation of complex quantitative traits in maize, mainly in tropical environments. In addition, our results will be very useful for assisting us in the assignment of heterotic groups and the selection of the best parental combinations for new breeding crosses, mapping populations, mapping synthetic populations, guiding crosses that target highly heterotic and yielding hybrids, and predicting untested hybrids in the public breeding program UFV.

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Genetic Diversity among Elite Sorghum Inbred Lines Assessed with DNA Markers and Pedigree Information

Crop Science ◽

10.2135/cropsci1996.0011183x003600050049x ◽

1996 ◽

Vol 36 (5) ◽

pp. 1385-1392 ◽

Cited By ~ 24

Author(s):

D. Ahnert ◽

M. Lee ◽

D. F. Austin ◽

C. Livini ◽

W. L. Woodman ◽

...

Keyword(s):

Genetic Diversity ◽

Dna Markers ◽

Inbred Lines ◽

Pedigree Information

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Molecular assessment of maize inbred lines (Zea mays L.) Using microsatellite markers

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v43i4.39151 ◽

2018 ◽

Vol 43 (4) ◽

pp. 533-542

Author(s):

Maniruzzaman ◽

MG Azam ◽

S Islam ◽

MG Hossain ◽

MM Rohman

Keyword(s):

Genetic Diversity ◽

Plant Breeding ◽

Genetic Relatedness ◽

Agricultural Research ◽

Inbred Lines ◽

The Other ◽

Diversity Analysis ◽

Genetic Diversity Analysis ◽

Maize Inbred Lines ◽

Maize Genotypes

Genetic diversity analysis and germplasm characterization are essential steps in plant breeding and molecular markers are proved tool to accomplish. The present study was undertaken at the Molecular Breeding Lab of Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI) to determine the genetic relatedness and molecular characterization of 15 maize inbred lines of BARI. In present study, genetic diversity analysis was performed by using 10 SSR primers to evaluate the polymorphisms, among them six primers showed distinct polymorphism between the maize inbred lines. The maize genotypes E81, E144, E08, E167, E102, E142 and E121 were found more diverged (0.9003) compared to other inbred lines. On the other hand, the lowest genetic distance values (0.1501) were found between the genotype E140 and genotype E80 followed by genotype E126 and genotype E140; genotype E140 and genotype E65; genotype E65 and genotype E80 values were identical (0.4502). The genotypes viz. E81, E144, E08, E167, E102, E142 and E121 were found far away from centroid of the cluster and rest of the genotypes were placed around the centroid. The Principal Coordinate Analysis (PCO) helped to visualize four major clusters and showed that seven maize inbred lines (E81, E58, E08, E167, E102, E142 and E121) were far away from the other genotypes. In conclusion, SSR markers enabled discrimination among accessions and provided valuable information for future use in improvement of these genomic resources.Bangladesh J. Agril. Res. 43(4): 533-542, December 2018

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Genetic diversity of oil-tea camellia germplasms revealed by ISSR analysis

International Journal of Biomathematics ◽

10.1142/s1793524515500709 ◽

2015 ◽

Vol 08 (05) ◽

pp. 1550070 ◽

Cited By ~ 2

Author(s):

Lan-Ying Zhou ◽

Xiang-Nan Wang ◽

Li-Ping Wang ◽

Yong-Zhong Chen ◽

Xiao-Cheng Jiang

Keyword(s):

Genetic Diversity ◽

Reaction System ◽

Inter Simple Sequence Repeat ◽

Genetic Distances ◽

Similarity Coefficient ◽

Hunan Province ◽

Information Index ◽

Group I ◽

Issr Pcr ◽

Simple Sequence

Genetic diversity of 51 oil-tea camellia germplasms was analyzed using the optimized inter-simple sequence repeat (ISSR)–PCR reaction system with 22 primers screened from a set of 100 ISSR primers. The results showed that 493 discernible loci with distinct electrophoretic bands were obtained, of which, 478 loci (96.78%) were polymorphic. This indicated that oil-tea germplasms possess abundant genetic diversities. By clustering analysis performed using softwares of NTSYS 2.10 and Winboot, 51 oil-tea germplasms were divided into two groups: Group I had 48 lines of Camellia oleifera Abel, while Group II had three C. oleifera Abel related species and their similarity coefficient was 0.62. Group I was further divided into Group I-1 and Group I-2, and their similarity coefficient (Gs) was 0.634. All members of Group I-1 originated from Hunan Province, while Group I-2 included the rest of Hunan lines and those originated from other regions of China. Analyzed by software POPGENE 1.32, the Shannon's information index (I*) of genetic polymorphism was 0.3852, the genetic diversity among different region populations (Ht) was 0.2537, the genetic diversity within populations (Hs) was 0.15545, the differentiation coefficient of genetic diversity among populations (Gst) was 0.3967, and the gene flow among populations (Nm*) was 0.8262. The Nei's genetic distances between the Hunan population and the populations originated from other regions of China implied that geographic isolation strongly influenced genetic differentiation among populations. Meanwhile, seedling rootstock grafting and high grafting for tree crown produced genetic variations among clonal offsprings.

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Characterisation of genetic diversity and DNA fingerprinting of Australian chickpea (Cicer arietinum L.) cultivars using MFLP markers

Australian Journal of Agricultural Research ◽

10.1071/ar07401 ◽

2008 ◽

Vol 59 (8) ◽

pp. 707 ◽

Cited By ~ 6

Author(s):

R. Lin ◽

H. Yang ◽

T. N. Khan ◽

K. H. M. Siddique ◽

G. Yan

Keyword(s):

Genetic Diversity ◽

Cicer Arietinum ◽

Dna Markers ◽

Fragment Length Polymorphism ◽

Grain Legume ◽

Dna Fingerprints ◽

Breeding Programs ◽

Cicer Arietinum L ◽

The World ◽

Chickpea Cultivars

Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite-anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identified and all were unequivocally identified using the DNA fingerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. The MFLP approach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identified will be useful for chickpea breeding programs in selecting parent materials.

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Genetic Diversity Analysis of Sugarcane Parents in Chinese Breeding Programmes Using gSSR Markers

The Scientific World JOURNAL ◽

10.1155/2013/613062 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

Qian You ◽

Liping Xu ◽

Yifeng Zheng ◽

Youxiong Que

Keyword(s):

Genetic Diversity ◽

Molecular Diversity ◽

Genetic Relationships ◽

Breeding Programs ◽

Sugarcane Varieties ◽

Breeding Station ◽

The World ◽

Breeding Programmes ◽

Genetic Structures ◽

Simple Sequence

Sugarcane is the most important sugar and bioenergy crop in the world. The selection and combination of parents for crossing rely on an understanding of their genetic structures and molecular diversity. In the present study, 115 sugarcane genotypes used for parental crossing were genotyped based on five genomic simple sequence repeat marker (gSSR) loci and 88 polymorphic alleles of loci (100%) as detected by capillary electrophoresis. The values of genetic diversity parameters across the populations indicate that the genetic variation intrapopulation (90.5%) was much larger than that of interpopulation (9.5%). Cluster analysis revealed that there were three groups termed as groups I, II, and III within the 115 genotypes. The genotypes released by each breeding programme showed closer genetic relationships, except the YC series released by Hainan sugarcane breeding station. Using principle component analysis (PCA), the first and second principal components accounted for a cumulative 76% of the total variances, in which 43% were for common parents and 33% were for new parents, respectively. The knowledge obtained in this study should be useful to future breeding programs for increasing genetic diversity of sugarcane varieties and cultivars to meet the demand of sugarcane cultivation for sugar and bioenergy use.

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