THE EFFECT OF STEEPING ROBUSTA COFFEE BEANS ON MONOCYTES: EXPRESSION OF IL-1β AND TNF-α AGAINST Streptococcus mutans

Adhesion, IL–1β, TNF–α are components that affect in inflammation. So, the effect of steeping green and black Robusta coffee beans to adhesion of Streptococcus mutans on this components. This study used monocytes isolated from healthy human peripheral blood using Ficoll-Hypaque centrifugation method. Monocytes were divided into eight groups, i. e. (i) Control group (untreated monocytes), (ii) Streptococcus mutans group (monocytes + S. mutans), (iii) Black Coffee 2.5 % group (monocytes + black coffee beans 2.5 % + S. mutans), (iv) Black Coffee 5 % group (monocytes + black coffee beans 5 % + S. mutans), (v) black Coffee 10 % group (monocytes + black coffee beans 10 % + S. mutans), (vi) Green Coffee 2.5 % group (monocytes + green coffee beans 2.5 % + S. mutans), (vii) Green Coffee 5 % group (monocytes + green coffee beans 5 % + S. mutans), (viii) Green coffee 10 % group (monocytes + green coffee beans 10 % + S. mutans). S. mutans adhesion on monocytes was analyzed using histochemistry method, while immunocytochemical staining was used for analyzing IL–1β and TNF–α. Cells counting was done per 100 monocytes under a light microscope with 400 × magnification. Data were analyzed using ANOVA followed by LSD test. Results showed that steeping green and black Robusta coffee beans increased the adhesion of S. mutans on monocytes, but it decreased of IL–1β, TNF–α expression (P < 0.05). In conclusion, steeping of green and black robusta coffee beans reduced inflammation against S. mutans.

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The Effect of Orlistat, Green Coffee Bean Extract, and Its Combinations on Lipid Profi le and Adiponectin Levels

Jurnal NERS ◽

10.20473/jn.v9i1.2957 ◽

2017 ◽

Vol 9 (1) ◽

pp. 26 ◽

Cited By ~ 1

Author(s):

Joko Setyono ◽

Dwi Adi Nugroho ◽

Mustofa Mustofa ◽

Saryono Saryono

Keyword(s):

Chlorogenic Acid ◽

Water Extract ◽

Ethanol Extract ◽

Control Group ◽

Coffee Bean ◽

Green Coffee ◽

Green Coffee Beans ◽

Coffee Beans ◽

Significant Difference ◽

Obese Rats

Introduction: Obesity prevalence is estimated increases, reached 19.1% of the population aged 15 years and over. This study aimed to determine the differences of the anti- obesity effect of orlistat, an extract of green coffee beans (Coffea canephora robusta), and its combination to the adiponectin levels and lipid profi le. Method: This research was true experimental post -test only with control group design with completely randomized design (CRD). Experimental animals (Rattus novergicus) were divided into 6 group, group 1 ( negative control ), group 2 ( positive control ), group 3 was group of obese rats fed orlistat dose of 15.9 mg/kg, group 4 was the group of obese rats were fed ethanol extract of green coffee beans dose of 400 mg/kg, group 5 was the group of obese rats were given water extract of green coffee beans dose of 400 mg/kg, and group 6 was group of obese rats were fed a combination of orlistat dose of 15.9 mg/kgand ethanol extract of green coffee beans at a dose of 400 mg/kg. Lipid profi les and adiponectin levels were measured with a spectrophotometer at 500nm absorbance. The data were analyzed by one-way ANOVA, and then post hoc Least Significant Difference (LSD) with α = 0.05. Result: Ethanol extract of green coffee is more effi cient in lowering LDL cholesterol, increasing HDL cholesterol, and lowering the total cholesterol levels on HFD diet-induced mice, but there was no difference in lowering triglycerides . The combination of ethanol extract of green coffee with orlistat showedthe increasing of adiponectin levels were highest than the other treatment groups. Discussion: The ethanol extract of green coffee readily diffuses through the digestive tract epithelium. Green coffee contains chlorogenic acid active compounds that can increase the body’s metabolism, increase fatty acid oxidation, reduce levels of triglycerides in the liver, and working to inhibit lipase and amylase pancreaticenzymes. In addition to chlorogenic acid, polyphenol content in coffee is also potentially reduce visceral fat accumulation. Preparations extract by ethanol allows the absorption process is done effi ciently and quickly.Keywords: obesity, orlistat, greencoffee, lipid profi le, adiponectin

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Study on the Postprandial Glucose Responses to a Chlorogenic Acid-Rich Extract of Decaffeinated Green Coffee Beans in Rats and Healthy Human Subjects

Food Science and Technology Research ◽

10.3136/fstr.18.849 ◽

2012 ◽

Vol 18 (6) ◽

pp. 849-860 ◽

Cited By ~ 22

Author(s):

Kazuya IWAI ◽

Yusaku NARITA ◽

Taiji FUKUNAGA ◽

Osamu NAKAGIRI ◽

Tomoyasu KAMIYA ◽

...

Keyword(s):

Chlorogenic Acid ◽

Human Subjects ◽

Postprandial Glucose ◽

Green Coffee ◽

Healthy Human ◽

Green Coffee Beans ◽

Coffee Beans ◽

Healthy Human Subjects

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Factors affecting the formation of acrylamide in coffee

Czech Journal of Food Sciences ◽

10.17221/10604-cjfs ◽

2004 ◽

Vol 22 (SI - Chem. Reactions in Foods V) ◽

pp. S22-S24 ◽

Cited By ~ 10

Author(s):

K. Bagdonaite ◽

M. Murkovic

Keyword(s):

Liquid Chromatography ◽

Great Influence ◽

Uv Detection ◽

Green Coffee ◽

Factors Affecting ◽

Robusta Coffee ◽

Green Coffee Beans ◽

Coffee Beans ◽

Significant Difference ◽

Roasting Time

Four types of green coffee beans (Robusta and Arabica) were roasted in a laboratory roaster and in an oven. The samples were analysed for acrylamide using liquid chromatography with UV detection. Significant difference in acrylamide content was observed in different coffee types. Robusta coffee beans, roasted to different degrees of browning contained more acrylamide than Arabica varieties. Roasting time and temperature had a great influence on the acrylamide formation in coffee beans. Coffee beans roasted for longer time had less acrylamide. Additionally, coffee beans roasted at higher temperatures contained less acrylamide compared to those roasted at lower temperatures.

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Evaluation of Renoprotective Effects of Our Locally Grown Green Coffee Beans against Cisplatin-Induced Nephrotoxicity in Swiss Albino Mice

International Journal of Nephrology ◽

10.1155/2021/2805068 ◽

2021 ◽

Vol 2021 ◽

pp. 1-8

Author(s):

Bati Leta ◽

Chala Kenenisa ◽

Tesaka Wondimnew ◽

Tariku Sime

Keyword(s):

Body Weight ◽

Serum Creatinine ◽

Control Group ◽

Green Coffee ◽

Kidney Weight ◽

Green Coffee Beans ◽

Coffee Beans ◽

Serum Blood Urea Nitrogen ◽

Locally Grown ◽

Dose Dependent

Introduction. Nephrotoxicity is the most common and severe side effect of cisplatin. Cisplatin causes nephrotoxicity through free radical production and debilitating cellular antioxidant capacity. Coffee is a commonly consumed drink and its ingredients have antioxidant roles that could bring benefits to patients affected by nephrotoxicity. Thus, the present study aimed to investigate the renoprotective effects of our locally grown green coffee beans against cisplatin-induced nephrotoxicity in Swiss albino mice. Methods. The posttest only control group design was employed on a total of thirty male Swiss albino mice. The mice were divided into five groups: group I (normal control group) received distilled water; group II (negative control group) received distilled water; and groups III–V (treatment groups) received 100, 200, and 300 mg/kg BW/day of green coffee bean extract for 14 days, respectively. Nephrotoxicity was induced in groups II–V by a single intraperitoneal injection of cisplatin (7.5 mg/kg). All mice were sacrificed after 14 days and blood was drawn to evaluate kidney function tests (serum creatinine and serum blood urea nitrogen). Besides, body weight, relative kidney weight, and kidney histopathology were investigated. Result. Our results showed that treatment of cisplatin alone (group II mice) significantly increased serum creatinine, serum blood urea nitrogen, relative kidney weight, and pathological damage to the kidney with a decrease in final body weight. However, low-dose green coffee beans (group III), medium-dose green coffee beans (group IV), and high-dose green coffee beans (group V) mice showed a significant dose-dependent decrease in serum creatinine, serum blood urea nitrogen, and relative kidney weight. Furthermore, the dose-dependent treatment with green coffee bean extract prevented the decrease in body weight gain and pathological damage to the kidney in mice. Conclusion. Our locally grown green coffee beans brought a dose-dependent ameliorative effect and a promising preventive approach against cisplatin-induced kidney damage in mice.

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Seduhan Bubuk Kopi Robusta Spray Dryed Meningkatkan Viabilitas Monosit yang Dipapar Eksotoksin Streptococcus mutans (In Vitro)

STOMATOGNATIC - Jurnal Kedokteran Gigi ◽

10.19184/stoma.v16i1.19956 ◽

2019 ◽

Vol 16 (1) ◽

pp. 16

Author(s):

Fiftiani Syarah ◽

I Dewa Ayu Susilawati ◽

Nadie Fatimatuzzahro

Keyword(s):

Free Radicals ◽

Streptococcus Mutans ◽

Cell Damage ◽

Human Peripheral Blood ◽

Gradient Centrifugation ◽

Control Group ◽

Control Group Design ◽

Robusta Coffee ◽

Coffee Brew

Monocytes response against bacterial exotoxins can cause cell damage, due to the production of antimicrobial compounds such as oxidants and free radicals. Spray dryed robusta coffee brewed contained antioxidants that was alleged to be able counteract oxidants and free radicals leading to prevent cell damage. This research aimed to prove the effect of spray dryed robusta coffee brew (SDRCB) to increase monocytes viability after being exposed by exotoxins of Streptococcus mutans (in vitro). This in vitro experimental study used the post test only control group design. The object of study was human peripheral blood monocytes, isolated by means of gradient centrifugation method. Monocyte viability was analyzed by trypan blue assay. The parameter was the percentage viable monocytes. Data were analyzed using Anova and LSD. Monocytes incubated with SDRCB showed a significant (p<05) higher viability (96.95%) than monocytes without SDRCB (94,27%). Spray dryed robusta coffee brew proved to be able increase viability of monocytes after S.mutans exposure (in vitro).

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