scholarly journals Anatomical features of conductive tissues of soybeans that prevent the penetration of pathogenic agents of bacterial blight into seeds

2021 ◽  
Vol 188 (4) ◽  
pp. 25-34
Author(s):  
S.V. Zelentsov ◽  
◽  
G.M. Saenko ◽  
E.V. Moshnenko ◽  
◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Pathogenic Bacteria ◽  
Vascular System ◽  
Sieve Tube ◽  
Soybean Seeds ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Sieve Plates ◽  
Soybean Leaves ◽  
Pathogenic Agents

The ways of penetration of pathogenic bacteria from the infected vegetative parts of plants into soybean seeds remain practically unexplored. It is widely believed that soybean seeds are infected through the vascular system from already infected areas of the vegetative parts. The aim of the present research was to study the possibility of penetration of pathogens of bacterial blight into soybean seeds through the conductive tissues of plants. The studies were carried out in 2019–2021 in V.S. Pustovoit All-Russian Research Institute of Oil Crops on plants and seeds of soybean variety Vilana. It was found that the size of stomatal slots in soybean leaves is 8–12 µm. This ensures free penetration of bacteria with a diameter of 1.3–1.7 µm into the leaf mesophyll. The pore sizes of the sieve plates of the phloem range from 0.4–0.7 to 0.8–1.6 µm, depending on the age of the plants. The largest pores of the phloem sieve plates are comparable to the diameters of pathogenic bacteria. However, a large number of transverse sieve plates located in the vessels of the phloem every 0.05–0.1 mm will filter and partially retain bacteria in each sieve tube along the path of cell sap in the phloem. Therefore, the pathogenic bacteria passing through the entire phloem from leaves infected with bacteriosis up to pods is physically unlikely. In pods, the vascular system ends in the area of attachment of the placenta to the seed hilum. In the hilum, there are no conductive tissues, and the further flow of water and nutrients into the seed is carried out diffusely through the plasmodesma of cell walls. It was found that the anatomical structure of the soybean phloem prevents the free movement of pathogenic bacteria along the conductive system directly into the inner tissues of the seeds. Therefore, the hypothesis of infection of soybean seeds with pathogens of bacterial blight through the conducting system of the plant should be considered untenable

scholarly journals The primary cause for the development of bacterial blight of seeds in soybean and other leguminous crops

2021 ◽  
Vol 185 (1) ◽  
pp. 73-89
Author(s):  
S.V. Zelentsov ◽  
◽  
G.М. Saenko ◽  
E.V. Moshnenko ◽  
E.N. Budnikov ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Secondary Infection ◽  
Acid Synthesis ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Immature Seeds ◽  
Necrotic Spots ◽  
Soybean Plants ◽  
Leguminous Crops ◽  
Mature Seeds

One of the significant reasons for the decrease in the sowing quality of seeds in leguminous crops all over the world is bacterial blight of seeds in the form of necrotic decaying spots on the outer or inner side of the cotyledons. A hypothesis was put forward about the presence of a common primary non-bacterial cause of the development of necrosis on cotyledons, regardless of the species, variety and growing zone. The studies were carried out in 2016– 2020 in V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar, on seeds of soybeans, common bean, chickpea, white and narrow-leaved lupines. On immature seeds of healthy soybean plants in the phases of full filling and the beginning of physiological maturation, bacterial blight of seeds are never observed. Secondary (rain) moistening of mature seeds leads to the development of cotyledon necrosis and a decrease in their germination in soybeans, common beans, chickpeas, white lupine and narrow-leaved lupine. The physiological reason for the formation of cotyledon necrosis during the secondary moistening of mature seeds is the death of the tissues of the cotyledons, moistened before the stage of nucleic acid synthesis, and unable to return to the dormant stage upon repeated drying. Symptoms of the development of cotyledon necrosis after secondary moistening of mature seeds, and bacterial blight of seeds, are practically identical in all leguminous crops. The common primary cause of the formation of necrotic spots on the surface of the cotyledons, identified as bacterial blight of seeds, regardless of the species of legumes, variety and ecological-geographical zone of cultivation, is the secondary (rain) moisture of seeds that have not yet been harvested in the field of mature plants. Isolation of bacterial pathogens of various species and families in necrotic areas of the cotyledons can be explained by secondary infection of already dead tissues. Therefore, the species composition of bacterial microflora in each case will be determined by its presence in the environment.

scholarly journals Sunflower diseases control by biological fungicides

2022 ◽  
Vol 42 ◽  
pp. 02014
Author(s):  
Nadezhda Bushneva ◽  
Alexander Bushnev
Keyword(s):  
Bacterial Blight ◽  
Organic Food ◽  
Seed Infection ◽  
Net Income ◽  
Sunflower Seeds ◽  
Dry Rot ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Different Strains ◽  
Russian Research

The demand for organic food has grown rapidly over the last 25 years in many countries of the world. Organic farming standards require the application of biological products of disease control. The article presents the material on studying the effectiveness of fungicides based on microbiological antagonists against sunflower diseases. We carried out the study in 2020-2021 at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) (Krasnodar). We established that the fungicides Geostim Fit type E, WS based on microbiological antagonists did not reduce the germination of sunflower seeds and actively suppressed seed infection. Their effectiveness against Alternaria spp. reached 50-74 %, against Rhizopus spp. – 74–100 %, against bacteria – 76–80 %. Double treatment of sunflower plants with BFTIM, WS (different strains) reduced the frequency of bacterial blight to 37 %, of dry rot – to 60 %, of Alternaria blight – to 39 %, of Phoma rot – to 41 %, and of Fusarium blight – to 75 %. The application scheme of Geostim Fit E (G), WS; BFTIM (E), WS; BFTIM (Zh), WS showed the best economic effectiveness with a seed yield of 3.56 t/ha, net income of 122 910 rubles, and profitability of 311 %.

scholarly journals Sieve tube structural variation in Austrobaileya scandens and its significance for lianescence

2021 ◽  
Author(s):  
Juan M Losada ◽  
Zhe He ◽  
Noel Michele Holbrook
Keyword(s):  
Structural Variation ◽  
Vascular System ◽  
Light And Electron Microscopy ◽  
Sieve Tube ◽  
Phloem Transport ◽  
Sieve Plate ◽  
Geometrical Properties ◽  
Mechanical Constraints ◽  
Vascular Elements ◽  
Sieve Plates

Lianas are characterized by large leaf areas and slender stems, a combination of features that require an efficient vascular system. The only extant member of the Austrobaileyaceae, is an endemic twining liana of the tropical Australian forests with well-known xylem hydraulic traits. However, the vascular phloem continuum through aerial organs remains understudied. We analyzed the structure of phloem conduits across leaf veins and stems of A. scandens, combining topological data obtained through light and electron microscopy, with current models of phloem transport. Leaves displayed a low xylem to phloem ratio compared with leaves of other angiosperms, with vascular elements invariant in diameter along the midrib, but tapered across vein hierarchies. Sieve plate pore radii were extremely small: 0.08μm in minor veins, increasing to 0.12μm in the petiole and only to 0.20μm at the base of the stem, tens of meters away. Searcher branches contained tube shaped phloem conduits with a pectin-rich wall, whereas twining stems displayed sieve elements with tangential connections that displayed a greater fraction of the tubes populated with an astonishing number of sieve plates. Hydraulic segmentation of the leaves in Austrobaileyaceae correlate with vesseless leaves that benefit photoassimilate export through volumetric scaling of the sieve tube elements. Yet, compared with canopy dominant trees, the geometrical properties of the sieve tube in twining stems, restrict considerably energy distribution in the sub-canopy layers, potentially favoring the allocation of assimilates toward the elongating branches. Thus, the conductive xylem of twining stems contrasts with a poorly conductive phloem that meets the mechanical constraints of lianescence.

scholarly journals Sieve tube structural variation in Austrobaileya scandens and its significance for lianescence

2021 ◽  
Author(s):  
Juan Losada ◽  
Zhe He ◽  
Noel Holbrook
Keyword(s):  
Resource Allocation ◽  
Hydraulic Resistance ◽  
Structural Variation ◽  
Vascular System ◽  
Sieve Tube ◽  
Sink Strength ◽  
Sieve Plate ◽  
Vascular Elements ◽  
Microscopy Analysis ◽  
Sieve Plates

Lianas combine large leaf areas with slender stems, features that require an efficient vascular system. The only extant member of the Austrobaileyaceae is an endemic twining liana of the tropical Australian forests with well-known xylem hydraulics, but the vascular phloem continuum aboveground remains understudied. Microscopy analysis across leaf veins and stems of A. scandens revealed a low foliar xylem to phloem ratio, with isodiametric vascular elements along the midrib, but tapered across vein orders. Small sieve plate pore radii increased from 0.08 µm in minor veins to 0.12 µm in the petiole, but only to 0.20 µm at the stem base, tens of meters away. In searcher branches, phloem conduits contained a pectin-rich wall and simple plates, whereas in twinning stems, conduits connected through highly-angled-densely populated sieve plates. Twisted and elongated stems of A. scandens display a high hydraulic resistance of phloem conduits, which decreases from leaves to stems, efficiently delivering photoassimilate from sources under Münch predictions. Sink strength of a continuously growing canopy might be stronger than in self-supporting understory plants, favoring resource allocation to aerial organs in angiosperms that colonized the vertical niche.

scholarly journals Genetic Characterization of Russian Rapeseed Collection and Association Mapping of Novel Loci Affecting Glucosinolate Content

Genes ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 926
Author(s):  
Rim Gubaev ◽  
Lyudmila Gorlova ◽  
Stepan Boldyrev ◽  
Svetlana Goryunova ◽  
Denis Goryunov ◽  
...  
Keyword(s):  
Association Mapping ◽  
Glucosinolate Content ◽  
Oilseed Crop ◽  
Genetic Determinants ◽  
Oil Crops ◽  
Genome Wide ◽  
Russian Research Institute ◽  
Independent Direction ◽  
Agronomical Traits

Rapeseed is the second most common oilseed crop worldwide. While the start of rapeseed breeding in Russia dates back to the middle of the 20th century, its widespread cultivation began only recently. In contrast to the world’s rapeseed genetic variation, the genetic composition of Russian rapeseed lines remained unexplored. We have addressed this question by performing genome-wide genotyping of 90 advanced rapeseed accessions provided by the All-Russian Research Institute of Oil Crops (VNIIMK). Genome-wide genetic analysis demonstrated a clear difference between Russian rapeseed varieties and the rapeseed varieties from the rest of the world, including the European ones, indicating that rapeseed breeding in Russia proceeded in its own independent direction. Hence, genetic determinants of agronomical traits might also be different in Russian rapeseed lines. To assess it, we collected the glucosinolate content data for the same 90 genotyped accessions obtained during three years and performed an association mapping of this trait. We indeed found that the loci significantly associated with glucosinolate content variation in the Russian rapeseed collection differ from those previously reported for the non-Russian rapeseed lines.

Vascular system of the floret of Alopecurus carolinianus (Gramineae)

1987 ◽  
Vol 65 (12) ◽  
pp. 2592-2600 ◽  
Author(s):  
Thompson Demetrio Pizzolato
Keyword(s):  
Vascular System ◽  
Sieve Tube ◽  
Sieve Element ◽  
Sieve Elements

The interconnecting vascular system of the floret of Alopecurus carolinianus Walter begins as a single, collateral bundle, which enters the rachilla and becomes reorganized into a diarch pattern while ascending between the glumes. During a pronounced posterior enlargement, the rachilla bundle becomes connected with the median and four lateral bundles of the lemma. Above the trace to the lemma median, elements of a xylem discontinuity surrounded by those of a sieve-element plexus form in the rachilla bundle. Higher, a trace consisting of elements of the xylem discontinuity and the plexus enters the anterior and the posterior stamen. Two bundles, the lowest portion of the pistil vasculature, rise eccentrically from the xylem discontinuity and sieve-element plexus at the level of the stamen traces. The bundles condense into one which rotates counterclockwise and connects with the anterior sieve tube of the pistil. The xylem discontinuity of the bundle now in the pistil begins to diminish, and the sieve elements fan out to the sides and posterior of the xylem discontinuity. From the sieve elements one or two posterolaterals emerge toward the styles. The bundle of diffuse sieve elements in a semicircle behind the diminishing xylem discontinuity is now the placental bundle of the pistil. After its xylem discontinuity and then its sieve elements fade out, the placental bundle merges with the ovule at the chalaza.

Seasonal Changes in Callose Levels and Fluorescein Translocation in the Phloem of Vitis Vinifera L.

IAWA Journal ◽  
1991 ◽  
Vol 12 (3) ◽  
pp. 223-234 ◽  
Author(s):  
Roni Aloni ◽  
Carol A. Peterson
Keyword(s):  
Vitis Vinifera ◽  
Sieve Tube ◽  
Growing Season ◽  
Early Spring ◽  
Vitis Vinifera L ◽  
First Year ◽  
Secondary Phloem ◽  
Radial Gradient ◽  
Sieve Tubes ◽  
Sieve Plates

The secondary phloem of Vitis vinifera L. is characterised by a radial gradient of sieve tube diameters. Sieve tubes maturing early in the growing season have the largest diameters; those maturing late in the season have the smallest. In early spring, masses of winter dormancy callose are gradually digested in a polar radial pattern, proceeding outwards from the cambium. The fluorescent dye, fluorescein, was used to detect translocation in sieve tubes. During spring, dye translocation was first observed in the wider sieve tubes produced near the end of the previous year and wh ich had reduced amounts of callose. But translocation was not observed in the very narrow sieve tubes formed at the end of the year although they were the first to be callose free. The reactivated sieve tubes functioned for about one month. New sieve tubes differentiated three weeks after dormancy callose breakdown and started to function about one week later, so that the transition of translocation activity from the sieve tubes of the previous year to those of the current year is relatively rapid. The sieve tubes formed toward the end of the growing season (but not the narrowest ones formed at the very end of the season) function during parts of two successive seasons, while the sieve tubes forrned early in the season usually function during the first year only. Callose amounts increase gradually during summer in both the old and new sieve tubes and become relatively heavy in the old ones. At this developmental stage, translocation occurs through young sieve plates with relatively high callose deposits.

scholarly journals The results of preliminary trial experimental hybrids of winter rapeseed bred at VNIIMK by economically valuable traits

2021 ◽  
Vol 188 (4) ◽  
pp. 35-40
Author(s):  
E.A. Strelnikov ◽  
◽  
E.B. Bochkaryova ◽  
L.A. Gorlova ◽  
V.V. Serdyuk ◽  
...  
Keyword(s):  
Central Zone ◽  
Glucosinolate Content ◽  
Brassica Napus L ◽  
Hybrid Combination ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Economically Valuable Traits ◽  
Winter Rapeseed ◽  
Standard Cultivar ◽  
First Time

The purpose of the research was a preliminary estimation of ten experimental hybrids of winter rapeseed (Brassica napus L.) bred in the V.S. Pustovoit All-Russian Research Institute of Oil Crops by the primary economically valuable traits. The research was conducted in the central zone of the Krasnodar region in 2019–2020. The results of the research are presented for the first time. On average for two years, nine of ten the best experimental hybrids increased by seeds yield by 0.32–0.76 t per ha both the standard cultivar Loris and the foreign hybrid Mercure. Middle seed yield of the studied hybrids varied significantly, variance range was from 4.99 t per ha (VN-3 × ОРK-10) to 5.62 t per ha (VN-4 × ОРK-20). Maximal values of seed and oil yields per a unit area were fixed for a hybrid combination VN-4 × ОРK-20. Except that, the hybrid is characterized with high weight of 1000 seeds (4.5 g). A hybrid combination VN-13 × ОРK-10 demonstrated high average by years seeds yield (5.33 t per ha) and oil yield (2.25 t per ha).Oil content in seeds and weight of 1000 seeds of this combination were at the level of the standard cultivar and the foreign hybrid. This hybrid shows the lowest glucosinolate content in seeds – 16.7 µmol/g, that is less that the foreign hybrid Mercure has by 2.3 µmol/g. The preliminary conclusion based on our researches is: the selected hybrids after confirmation of the competitive trial results can be of a great interest for production.

scholarly journals The number of glandular trichomes on the peduncles of true lavender inflorescences as an additional breeding trait for essential oil

2021 ◽  
Vol 188 (4) ◽  
pp. 41-52
Author(s):  
S.V. Zelentsov ◽  
◽  
E.V. Moshnenko ◽  
T.P. Shuvaeva ◽  
I.V. Gaytotina ◽  
...  
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Significant Contribution ◽  
Distribution Density ◽  
Glandular Trichomes ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Average Size ◽  
Selection Of ◽  
Russian Research

The main receptacle of essential oil in true lavender is the peltate glandular trichomes of the calyxes in the whorls of the inflorescences. Their average size is 175 ± 25 µm, in some cases – up to 250 µm. For the extraction of lavender oil, not only the calyxes are used, but the whole inflorescences including the flowering shoots. The surface of the peduncles of lavender inflorescences is also covered with peltate glandular trichomes. However, their contribution to the total volume of essential oil in the inflorescence has almost never been determined. The aim of this research was to study the distribution density of glandular trichomes within the inflorescence and to determine the proportion of the contribution of flowering trichomes to the formation of essential oil in the inflorescence. The research was carried out in 2021 on the basis of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in two ecological and geographical points of the Krasnodar region. The object of the study was the true lavender varieties Voznesenskaya 34, Rannyaya, Yuzhanka and Voznesenskaya Aroma. It was found that the size of glandular trichomes on peduncles of true lavender is 90 ± 15 µm. Their number on peduncles, depending on the variety, varies from 2141 to 3003 pcs. The density of distribution of glandular trichomes on the surface of peduncles is equal to 8.60–14.93 pcs/mm3 . The total volume of essential oil in all glandular trichomes of peduncles is 0.41–0.57 cmm . The total volume of essential oil in the inflorescences varied from 2.28 to 5.15 cmm . The share of essential oil in the glandular trichomes of the peduncles in relation to the entire inflorescence ranged from 9.33 to 19.56%. It is concluded that peltate glandular trichomes on flower-bearing axes make a significant contribution to the essential oil content of lavender inflorescences. For the selection of true lavender to increase the essential oil content and the yield of essential oil, an additional selection trait is proposed – the amount of glandular trichomes on the surface of peduncles, which can be regulated by changing their distribution density on the surface of peduncles, or increasing the length of inflorescences.

scholarly journals The impact of morphometric characteristics of plants self-pollinated sunflower lines on its lodging

2020 ◽  
Vol 4 (184) ◽  
pp. 3-11
Author(s):  
B.N. Bochkaryov ◽  
◽  
N.V. Medvedeva ◽  
E.N. Ryzhenko ◽  
◽  
...  
Keyword(s):  
Root System ◽  
Lateral Roots ◽  
Root Systems ◽  
Morphometric Characteristics ◽  
Main Root ◽  
Lodging Resistance ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Top Soil ◽  
The Impact

We carried out the research in 2018-2019 at the experimental station of V.S. Pustovoit All-Russian Research Institute of Oil Crops. The aim of the research is to study the effect of certain morphometric characteristics of the overground part of plants and the architectonics of the root system on the sunflower lodging. We found significant differences in the architectonics of root systems in 17 maternal lines of sunflower: we identified three morphotypes, differing in the number and thickness of lateral roots of the first and subsequent orders, located in the top soil. We identified the sunflower lines that have a root system with a well-developed main root and many lateral roots of various orders (type A), lines with a normally developed main root and a small number of lateral roots (type B), and lines with a poorly developed main root and few lateral roots in top soil (type C). We identified the presence of both low and high lodging in sunflower lines with different types of root systems. At the same time, there is a tendency towards higher lodging in lines with root system types B and C. The line SL12 3660 showed the maximum lodging resistance during two years of observations. It may be of interest for further work as a possible source of a lodging resistance trait.

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