scholarly journals Sunflower diseases control by biological fungicides

2022 ◽  
Vol 42 ◽  
pp. 02014
Author(s):  
Nadezhda Bushneva ◽  
Alexander Bushnev

The demand for organic food has grown rapidly over the last 25 years in many countries of the world. Organic farming standards require the application of biological products of disease control. The article presents the material on studying the effectiveness of fungicides based on microbiological antagonists against sunflower diseases. We carried out the study in 2020-2021 at V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK) (Krasnodar). We established that the fungicides Geostim Fit type E, WS based on microbiological antagonists did not reduce the germination of sunflower seeds and actively suppressed seed infection. Their effectiveness against Alternaria spp. reached 50-74 %, against Rhizopus spp. – 74–100 %, against bacteria – 76–80 %. Double treatment of sunflower plants with BFTIM, WS (different strains) reduced the frequency of bacterial blight to 37 %, of dry rot – to 60 %, of Alternaria blight – to 39 %, of Phoma rot – to 41 %, and of Fusarium blight – to 75 %. The application scheme of Geostim Fit E (G), WS; BFTIM (E), WS; BFTIM (Zh), WS showed the best economic effectiveness with a seed yield of 3.56 t/ha, net income of 122 910 rubles, and profitability of 311 %.

2021 ◽  
Author(s):  
L.A. Datsenko ◽  

We carried out analysis of the literature data on the knowledge level of sunflower dry rot in the laboratory of biomethod of the crop management department of V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK). As a result of the analysis, we studied the following issues: the disease pathogen; symptoms of manifestation; the disease prevalence in the world and in Russia; its harmfulness; biology; species composition and taxonomic position of fungi of the genus Rhizopus on sunflower; methods of artificial infection of sunflower with dry rot.


Author(s):  
S.V. Zelentsov ◽  
◽  
E.V. Moshnenko ◽  
T.P. Shuvaeva ◽  
I.V. Gaytotina ◽  
...  

The main receptacle of essential oil in true lavender is the peltate glandular trichomes of the calyxes in the whorls of the inflorescences. Their average size is 175 ± 25 µm, in some cases – up to 250 µm. For the extraction of lavender oil, not only the calyxes are used, but the whole inflorescences including the flowering shoots. The surface of the peduncles of lavender inflorescences is also covered with peltate glandular trichomes. However, their contribution to the total volume of essential oil in the inflorescence has almost never been determined. The aim of this research was to study the distribution density of glandular trichomes within the inflorescence and to determine the proportion of the contribution of flowering trichomes to the formation of essential oil in the inflorescence. The research was carried out in 2021 on the basis of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in two ecological and geographical points of the Krasnodar region. The object of the study was the true lavender varieties Voznesenskaya 34, Rannyaya, Yuzhanka and Voznesenskaya Aroma. It was found that the size of glandular trichomes on peduncles of true lavender is 90 ± 15 µm. Their number on peduncles, depending on the variety, varies from 2141 to 3003 pcs. The density of distribution of glandular trichomes on the surface of peduncles is equal to 8.60–14.93 pcs/mm3 . The total volume of essential oil in all glandular trichomes of peduncles is 0.41–0.57 cmm . The total volume of essential oil in the inflorescences varied from 2.28 to 5.15 cmm . The share of essential oil in the glandular trichomes of the peduncles in relation to the entire inflorescence ranged from 9.33 to 19.56%. It is concluded that peltate glandular trichomes on flower-bearing axes make a significant contribution to the essential oil content of lavender inflorescences. For the selection of true lavender to increase the essential oil content and the yield of essential oil, an additional selection trait is proposed – the amount of glandular trichomes on the surface of peduncles, which can be regulated by changing their distribution density on the surface of peduncles, or increasing the length of inflorescences.


Author(s):  
S.V. Zelentsov ◽  
◽  
G.M. Saenko ◽  
E.V. Moshnenko ◽  
◽  
...  

The ways of penetration of pathogenic bacteria from the infected vegetative parts of plants into soybean seeds remain practically unexplored. It is widely believed that soybean seeds are infected through the vascular system from already infected areas of the vegetative parts. The aim of the present research was to study the possibility of penetration of pathogens of bacterial blight into soybean seeds through the conductive tissues of plants. The studies were carried out in 2019–2021 in V.S. Pustovoit All-Russian Research Institute of Oil Crops on plants and seeds of soybean variety Vilana. It was found that the size of stomatal slots in soybean leaves is 8–12 µm. This ensures free penetration of bacteria with a diameter of 1.3–1.7 µm into the leaf mesophyll. The pore sizes of the sieve plates of the phloem range from 0.4–0.7 to 0.8–1.6 µm, depending on the age of the plants. The largest pores of the phloem sieve plates are comparable to the diameters of pathogenic bacteria. However, a large number of transverse sieve plates located in the vessels of the phloem every 0.05–0.1 mm will filter and partially retain bacteria in each sieve tube along the path of cell sap in the phloem. Therefore, the pathogenic bacteria passing through the entire phloem from leaves infected with bacteriosis up to pods is physically unlikely. In pods, the vascular system ends in the area of attachment of the placenta to the seed hilum. In the hilum, there are no conductive tissues, and the further flow of water and nutrients into the seed is carried out diffusely through the plasmodesma of cell walls. It was found that the anatomical structure of the soybean phloem prevents the free movement of pathogenic bacteria along the conductive system directly into the inner tissues of the seeds. Therefore, the hypothesis of infection of soybean seeds with pathogens of bacterial blight through the conducting system of the plant should be considered untenable


Author(s):  
S.V. Zelentsov ◽  
◽  
G.М. Saenko ◽  
E.V. Moshnenko ◽  
E.N. Budnikov ◽  
...  

One of the significant reasons for the decrease in the sowing quality of seeds in leguminous crops all over the world is bacterial blight of seeds in the form of necrotic decaying spots on the outer or inner side of the cotyledons. A hypothesis was put forward about the presence of a common primary non-bacterial cause of the development of necrosis on cotyledons, regardless of the species, variety and growing zone. The studies were carried out in 2016– 2020 in V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar, on seeds of soybeans, common bean, chickpea, white and narrow-leaved lupines. On immature seeds of healthy soybean plants in the phases of full filling and the beginning of physiological maturation, bacterial blight of seeds are never observed. Secondary (rain) moistening of mature seeds leads to the development of cotyledon necrosis and a decrease in their germination in soybeans, common beans, chickpeas, white lupine and narrow-leaved lupine. The physiological reason for the formation of cotyledon necrosis during the secondary moistening of mature seeds is the death of the tissues of the cotyledons, moistened before the stage of nucleic acid synthesis, and unable to return to the dormant stage upon repeated drying. Symptoms of the development of cotyledon necrosis after secondary moistening of mature seeds, and bacterial blight of seeds, are practically identical in all leguminous crops. The common primary cause of the formation of necrotic spots on the surface of the cotyledons, identified as bacterial blight of seeds, regardless of the species of legumes, variety and ecological-geographical zone of cultivation, is the secondary (rain) moisture of seeds that have not yet been harvested in the field of mature plants. Isolation of bacterial pathogens of various species and families in necrotic areas of the cotyledons can be explained by secondary infection of already dead tissues. Therefore, the species composition of bacterial microflora in each case will be determined by its presence in the environment.


2021 ◽  
Vol 32 ◽  
pp. 02011
Author(s):  
Oksana Serdyuk ◽  
Victoria Trubina ◽  
Lyudmila Gorlova

The purpose of the research was to determine the effect of herbicides on winter false flax and to identify the preparation that does not effect negatively on crop plants. The research was carried out in 20182020 at V.S. Pustovoit All-Russian Research Institute of Oil Crops. The experiment scheme included herbicides with active ingredients (a.i.), which effectively decreased the number of weeds on the plots. The effectiveness of the preparations was more than 70% for different types of weeds. However, the variants with the application of preparations with a.i. clopyralid 300 g/l, S-Metolachlor 960 g/l, ethametsulfuronmethyl 750 g/kg significantly decreased the plant density (by 18-32 pcs/m2) and seed yield (by 0.33-0.52 t/ha) of false flax in comparison with the control. The oil content of seeds was significantly decreased in the variants with the herbicides with a.i. S-Metolachlor 960 g/l with the application rate of 1.6 l/ha and ethametsulfuron-methyl 750 g/kg (by 1.2-1.5 %). In other variants, the oil content of false flax seeds differed from the control insignificantly (by 0.3-0.4 %). It has been established that the preparation with a.i. quinmerac 83 g/l + metazachlor 333 g/l with the application rate of 2.0 or 2.5 l/ha should be applied to decrease the number of weeds in the sowings of winter false flax in the central zone of the Krasnodar region. This preparation, without having a toxic effect, increases the yield by 0.15-0.17 t/ha and does not decrease the plant density and oil content of false flax seeds.


Author(s):  
A.D. Bochkovoy ◽  
◽  
V.I. Khatnyansky ◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
...  

The researches were conducted on fields of OOO NPO “Triumph”, the Matveevo-Kurgansky district of Rostov region and V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar in 2017–2020. The purpose of the research was to develop methodology of selection of self-fertile sunflower genotypes in foundation and breeder seed production. Our work resulted in development of improved population Dobrynya plus. Middle self-fertility of this population at self-pollination was 13.7% vs. 5.2% of the initial variety (increased by 2.6 times), a ratio of the most valuable genotypes with amount of fully formed seeds more than 150 seeds per a plant increased from 13.5 to 26.4% (by 1.9 times). At the open flowering and free insect pollination, population Dobrynya plus does not differ by its economically valuable traits from the initial variety. But at the close flowering, without insects pollination, yield of the population Dobrynya plus was 0.93 t per ha vs. 0.52 t per ha of the initial variety (increase by 1.8 times). At selection of elite seeds from sunflower varietal populations for seed growing, the most perspective are the biotypes with high level of self-fertility which traits are held stable in the different environments. Improvement of sunflower variety by 11 self-fertility does not lead to decreasing of its yield qualities by the main economically valuable traits. The obtained experimental data prove a prospectivity of researches directed on improvement of sunflower variety populations by self-fertility at production of foundation and breeder seeds and possibility to reach successful results.


Author(s):  
E.B. Bochkaryova ◽  
◽  
L.A. Gorlova ◽  
E.A. Strelnikov ◽  
V.V. Serdyuk ◽  
...  

Breeding of winter rapeseed in the V.S. Pustovoit All-Russian Research Institute of Oil Crops was started at the end of 60th of previous century with a selection of perspective winter forms from hybrids of spring brown mustard and winter rapeseed. Heterosis in intraspecific hybrids was studied. The best intraspecific hybrids exceeded the parental forms by 20–36% by seed yield, by 20–27% by green mass yield. A possibility to create intervarietal hybrids based on heterostyly was studied. Conditions for plants acclimation to increase cold resistance of winter rapeseed were studied; methods of selection for cold resistance were developed. In 1982, the development of rapeseed and turnip rape cultivars of ‘00’ type began. To create diversity of breeding germplasm, a great amount of samples from European counties that actively deals with rapeseed breeding were included into the work. In the V.S. Pustovoit All-Russian Research Institute of Oil Crops, the methods of estimation of oil and fodder protein quality were perfected, cultivars of rapeseed and turnip rape of ‘00’ type (e.g. erucic acid free and with low glucosinolate content) were developed. As a result of breeding for oil quality, high oleic cultivars were developed, that not only improve upon the oil nutritive efficiency but open new possibilities of its technical uses due to increasing oxi- and thermostability. The first Russian high oleic winter rapeseed cultivar Olivin was developed in the V.S. Pustovoit AllRussian Research Institute of Oil Crops and introduced in the State Variety Commission in 2019. The linear cultivars of winter rapeseed were developed by inbreeding, the first interlinear hybrids – using a CMS Ogura system. One of them named Debyut was introduced in the State variety trials 2020.


Author(s):  
G.N. Kuznetsova ◽  
◽  
R.S. Polyakova ◽  

In 2020, we submitted the spring rapeseed variety Sibiryak 60 bred at the Siberian Experimental Station (branch of V.S. Pustovoit All-Russian Research Institute of Oil Crops) to the State Commission of the Russian Federation on testing and protection of the selection achievements. We developed the variety by the method of multiple individual-family selection from the variety Avangard (All-Russian Research Institute of Rapeseed, Lipetsk). In 2012, there was identified an elite plant and further breeding work was carried out to improve the main economic traits: early maturity, productivity, oil content, resistance to lodging, seed falling and to the main pathogens. The general characteristics of the variety are high seed productivity and low content of glucosinolates in seeds. According to the results of the competitive variety trial (2018–2020), the spring rapeseed variety Sibiryak 60 exceeded the standard variety Granit in seed yield by 0.29 t/ha and in oil yield by 0.12 t/ha. In the conditions of the Krasnodar region, the variety Sibiryak 60 exceeded the standard variety Granit in seed yield by 0.31 t/ha, in the conditions of the Lipetsk region – by 0.30 t/ha. The new variety is meant to for cultivation for seeds in the Volgo-Vyatka (4), Middle Volga (7), Ural (9), West Siberian (10), and East Siberian (11) regions. The originator of the variety Sibiryak 60 is V.S. Pustovoit All-Russian Research Institute of Oil Crops.


Author(s):  
D.L. Savichenko ◽  
◽  
S.Z. Guchetl ◽  
A.V. Golovatskaya ◽  
◽  
...  

Studying of sunflower lines resistant to broomrape race G, bred in V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), Krasnodar showed the genetic control of resistance is monogenic with incomplete dominance. The purpose of the research was to seek and analyze molecular markers for the genes of resistance to broomrape race G using PCRmethods. In our research we used six developed in VNIIMK lines-donors resistant to broomrape race G: RGP1, RGP2, RGB, RGL1, RGL2, RGA, and susceptible lines VK 101, VK 678, VK 680. We extracted DNA from the leaves of young sunflower plants using STAB-buffer. For PCR-analysis we used 17 pairs of primers of two types: SCAR (sequence characterized amplified region) and SSR (simple sequence repeat). Conditions of amplification are as recommended by authors of markers with experimental selection of a temperature for primers hybridization. Within the research we balanced involved into work SSR- and 12 SCAR-loci using BLAST and a set of HanXRQr1.0. Due to these data we composed a physical map of loci location. After assessment of six resistant and three susceptible parental lines, seven markers demonstrated polymorphism by a length of locus DNA and two ones – by presence/absence of amplified fragment. For markers ORS 683 and ORS 1112 we observed a relation between loci polymorphism and susceptibility of parental lines to broomrape. This was certified with a presence of an allele of 364 n.p. length in ORS 683 and an absence of an allele of 375 n.p. length in ORS 1112. These markers were noted as primary ones for hybridological analysis in F1 и F2 with selected pairs for crossing. Additional markers will be selected individually using obtained data on loci polymorphism. Thus, as a result of the research we created a physical map for the further markers selection, found hybrid combinations and markers from the studied ones for hybridological analysis.


Sign in / Sign up

Export Citation Format

Share Document