257 Standardized ileal digestible amino acids and digestible energy contents in high-protein distiller's dried grains with solubles fed to growing pigs

2017 ◽  
Vol 95 (suppl_2) ◽  
pp. 124-124
Author(s):  
Y. Rho ◽  
C. Zhu ◽  
E. Kiarie ◽  
C. F. M. de Lange
2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Michelina Crosbie ◽  
Cuilan Zhu ◽  
Anna K Shoveller ◽  
Lee-Anne Huber

Abstract Two experiments were conducted to determine standardized ileal digestibility (SID) of amino acids (AA; Exp. 1) and net energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) samples [full fat (FF; 42.5% crude protein (CP), as-fed) and defatted (DF; 40.8% CP; as-fed)] for growing pigs. Two cornstarch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used, and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy (DE) by difference method. In each experiment, pigs were fed 2.8 × estimated maintenance energy requirement. In Exp. 1, eight ileal-cannulated barrows (25.1 ± 0.41 kg initial body weight) were used in a replicated 2 × 2 Latin square design (n = 8). In each period, pigs were adapted to diets for 5 d followed by 2 d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, eight barrows [23.4 ± 0.54 kg initial body weight (BW)] were used in a partially replicated Latin square design (n = 8). In each period, pigs were adapted to diets for 7 d, followed by 5 d of total urine collection and fecal grab sampling. The SID of CP (80.6 ± 1.1%) and Lys (88.0 ± 1.4%) were not different between FF and DF BSFLM. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P = 0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible energy (4,927 vs. 3,941 ± 75 kcal/kg), metabolizable energy (4,569 vs. 3,396 ± 102 kcal/kg), and predicted net energy (3,477 vs. 2,640 ± 30 kcal/kg, using equations from Noblet; 3,479 vs. 2,287 ± 28 kcal/kg, using equations from Blok, respectively) were greater for the FF versus the DF BSFLM (P < 0.05). The apparent total tract digestibility of neutral detergent fiber and acid detergent fiber were greater for the FF versus the DF BSFLM (P ≤ 0.05). Both FF and DF BSFLM had high SID for most AA; however, FF BSFLM was a better source of net energy for growing pigs. Therefore, both FF and DF BSFLM could be used as protein alternatives in growing pig diets.


1990 ◽  
Vol 50 (1) ◽  
pp. 155-164 ◽  
Author(s):  
T. C. Wang ◽  
M. F. Fuller

ABSTRACTSix diets based on maize and soya-bean meal were prepared. The basal diet had a crude protein (CP) concentration of 171 g/kg. The same ingredients, together with maize starch, were used to give a diet with CP 131 g/kg. This diet was supplemented with all (positive control) or with three of the four amino acids lysine, threonine, tryptophan and methionine. Each diet was given at two rates of intake. From the results, the optimum ratio between these four amino acids at different planes of intake was calculated. Amino acid digestibility in the basal diet was measured at three intakes, using pigs fitted with T cannulas at the terminal ileum.The results showed that the optimum ratios between the four amino acids (expressed in terms of digestible amino acids) were not affected by the rate of food intake. The optimum ratio between the four amino acids (i.e. when they are equally limiting) was lysine 1·00, methionine + cystine 0-61, threonine 0·64 and tryptophan 0·20. Digestibility was not affected by the level of intake.


1994 ◽  
Vol 72 (2) ◽  
pp. 221-241 ◽  
Author(s):  
R. J. Van Barneveld ◽  
E. S. Batterham ◽  
B. W. Norton

Three experiments were conducted to examine the effect of heating field peas (Pisum sativum cultivar Dundale) on (1) proximate analysis and total amino acid composition, (2) ileal and faecal digestibilities of amino acids, and (3) digestible energy content. Alternative techniques for assessing ileal and faecal digestibilities and digestible energy respectively, were also investigated. Forced-air dehydrators were used to heat field peas at temperatures of 110°, 135°, 150° or 165°. In the first experiment the apparent ileal and faecal digestibilities of amino acids and the faecal digestibility of energy in the raw and heated field peas were determined using pigs fitted with ‘T’ -shaped cannulas. In the second, apparent ileal digestibility of amino acids and the faecal digestibility of energy were determined using the direct ileal and rectal sampling technique. This involved a single collection of digesta and faeces from the digestive tract of the pig while it was anaesthetized. The faecal digestibilities of amino acids and energy were determined using total faeces collection in the third experiment. In all experiments the respective field-pea treatments comprised 400 g/kg sugar-based diets and were the only source of amino acids. Heat significantly decreased the lysine (14.6–8.7 g/kg; P < 0.001), cystine (3.2–2.6 g/kg; P < 0.01) and arginine (16.7–14.5 g/kg; P < 0.05) contents of the heated peas. The ‘reactive’ lysine content of the field peas, as measured using the Silcock technique, was decreased by 0.11 and 0.30 with the application of heat at 150° and 165° respectively. Heat treatments did not alter the ileal digestibility of most amino acids. Only aspartic acid (0.72–0.58), glutamic acid (0.80–0.65) and the basic amino acids, lysine (0.79–0.56) and arginine (0.85–0.75), showed a significant linear decrease (P < 0.05) in ileal digestibility over the heat treatments, determined using the ileal cannulation procedure. Heating significantly (P < 0.05) decreased faecal digestibility for all amino acids. Faecal digestibility was consistently greater than ileal digestibility for the raw field peas; however, this difference decreased with heat application until faecal digestibility was equal or less than ileal digestibility at the 165° treatment. Heat linearly depressed digestible energy, diet dry-matter digestibility and diet energy digestibility. Losses in lysine, cystine and arginine are likely to be due to early and advanced Maillard reactions. Considerable binding of the remaining lysine also occurred as indicated by a decline in Silcock-reactive lysine. The results indicate that the direct ileal sampling technique is a viable alternative to the cannulation procedure for amino acids, but further method development is required to decrease the variability associated with measurements. The estimation of faecal digestibility using indigestible markers and the partial faeces collection technique was as efficient as total faeces collection. In general, ileal digestibility of amino acids showed little response to heating, however, any changes that were observed were greatest for lysine. In contrast, faecal digestibility of all amino acids was greatly reduced with increasing heat application. This response appeared to be largely due to the effect of heating on microbial degradation and synthesis of amino acids in the hind-gut, rather than a reflection of the changes within the protein induced by heating. This variable response makes faecal digestibility an unreliable estimator of amino acid ileal digestibility.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 148-148
Author(s):  
Jessica P Acosta Medellin ◽  
Su A Lee ◽  
Hans H Stein

Abstract The objective of this experiment was to test if standardized ileal digestibility (SID) of AA in a new source of high-protein distillers dried grains with solubles (HP-DDGS) is greater than in conventional distillers dried grains with solubles (DDGS-CV). Nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicate 3 × 3 Latin Square design with 3 diets and 3 periods. Two diets included HP-DDGS or DDGS-CV as the sole source of CP and AA. A nitrogen-free diet was used to determine basal endogenous losses of CP and AA. The statistical model included ingredient as fixed effect and square, period, and animal as random effects. Results indicated that that HP-DDGS contained more CP and AA compared with DDGS-CV (Table 1). The SID of all AA in HP-DDGS were greater (P &lt; 0.001) compared with DDGS-CV with the exception that the SID of Pro was not different between the 2 sources of DDGS. The greater concentrations of AA and values for SID of AA in HP-DDGS indicate that this new DDGS source has a very high quality and may be a great source of digestible AA in diets fed to pigs.


2002 ◽  
Vol 82 (3) ◽  
pp. 385-391 ◽  
Author(s):  
Y. -L. Yin ◽  
Nar K. Gurung ◽  
E. A. Jeaurond ◽  
P. H. Sharpe ◽  
C. F. M. de Lange

Fecal digestible energy (DE) and ileal digestible crude protein (CP) and amino acid (AA) contents in sorghum, pear millet, high-oil corn, high-oil-high-protein corn, regular corn I and II were determined in growing pigs fitted with ileal “T ”cannulas. The cereal grain samples were grown in Canada and supplemented with vitamins and minerals to produce six experimental diets. Six pigs, with an average initial body weight of 17 kg, were fed the diets during six subsequent experimental periods according to a Latin square design. Pigs were fed twice daily at 2.6 times maintenance energy requirements. After a 7-d adjustment period, feces were collected for 3 d, followed by a 48-h collection of ileal digesta. Apparent and standardized ileal AA digestibilities were calculated. The high-oil corn and high-oil-high-protein corn had the highest (P < 0.05) DE content [4029, 4048 kcal kg-1 dry matter (DM), respectively]. The sorghum had a higher (P < 0.05) DE content (3941 kcal kg-1 DM) than pear millet (3603 kcal kg-1 DM) and regular corn I (3710 kcal kg-1 DM basis) and similar DE content to regular corn II (3945 kcal kg-1 DM). Contents of standardized ileal digestible CP and AA varied between cereal grains were generally highest (P < 0.05) for PM (CP: 101.6 g kg-1 DM; lysine, methionine plus cysteine, threonine: 3.4, 4.8 and 4.3 g kg-1 DM, respectively), HOPC (CP: 100.9 g kg-1 DM; lysine, methionine plus cysteine, threonine: 3.1, 5.4 and 4.3 g kg-1 DM, respectively) and similar for the other cereal grains (CP: 94.1–98.4 g kg-1 DM; lysine, methionine plus cysteine, threonine: 2.6–3.0, 3.4–4.3 and 3.3–4.0 g kg-1 DM, respectively). Differences in feeding values of these cereal grains should be considered when evaluating their potential inclusion in pig diets. Key words: Grains, pigs, energy, amino acids, digestibility


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