scholarly journals Controlling Nanoemulsion Surface Chemistry with Poly(2-Oxazoline) Amphiphiles

2018 ◽  
Author(s):  
Daniel A. Estabrook ◽  
Amanda F. Ennis ◽  
Rachael Day ◽  
Ellen Sletten
Keyword(s):  
Block Copolymers ◽  
Surface Chemistry ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Surface Functionalization ◽  
Covalent Modification ◽  
Dynamic Materials ◽  
Structure Activity ◽  
Modified Surface ◽  
Surface Chemistries

Emulsions are dynamic materials that have been extensively employed within pharmaceutical, food and cosmetics industries. However, their use beyond conventional applications has been hindered by difficulties in surface functionalization, and an inability to selectively control physicochemical properties. Here, we employ custom poly(2-oxazoline) block copolymers to overcome these limitations. We demonstrate that poly(2-oxazoline) copolymers can effectively stabilize nanoscale droplets of hydrocarbon and perfluorocarbon in water. The living polymerization allows for the incorporation of chemical handles into the surfactants such that covalent modification of the emulsion surfaces can be performed. Through post-emulsion modification, we are able to access nanoemulsions with modified surface chemistries, yet consistent sizes. By decoupling size and surface charge, we explore structure-activity relationships involving the cellular uptake of nanoemulsions.

scholarly journals Controlling Nanoemulsion Surface Chemistry with Poly(2-Oxazoline) Amphiphiles

2018 ◽  
Author(s):  
Daniel A. Estabrook ◽  
Amanda F. Ennis ◽  
Rachael Day ◽  
Ellen Sletten
Keyword(s):  
Block Copolymers ◽  
Surface Chemistry ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Surface Functionalization ◽  
Covalent Modification ◽  
Dynamic Materials ◽  
Structure Activity ◽  
Modified Surface ◽  
Surface Chemistries

Emulsions are dynamic materials that have been extensively employed within pharmaceutical, food and cosmetics industries. However, their use beyond conventional applications has been hindered by difficulties in surface functionalization, and an inability to selectively control physicochemical properties. Here, we employ custom poly(2-oxazoline) block copolymers to overcome these limitations. We demonstrate that poly(2-oxazoline) copolymers can effectively stabilize nanoscale droplets of hydrocarbon and perfluorocarbon in water. The living polymerization allows for the incorporation of chemical handles into the surfactants such that covalent modification of the emulsion surfaces can be performed. Through post-emulsion modification, we are able to access nanoemulsions with modified surface chemistries, yet consistent sizes. By decoupling size and surface charge, we explore structure-activity relationships involving the cellular uptake of nanoemulsions.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

scholarly journals Nanocarriers based on block copolymers of l-proline and lactide: the effect of core crosslinking versus its pH-sensitivity on their cellular uptake

2021 ◽  
pp. 110572
Author(s):  
Marek Brzeziński ◽  
Bartłomiej Kost Investigatio ◽  
Weronika Gonciarz ◽  
Agnieszka Krupa ◽  
Marta Socka ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Cellular Uptake ◽  
Ph Sensitivity

scholarly journals Films and Materials Derived from Aminomalononitrile

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 82
Author(s):  
Helmut Thissen ◽  
Richard A. Evans ◽  
Vincent Ball
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
State Of The Art ◽  
Chemical Reactivity ◽  
The State ◽  
Deposition Kinetics ◽  
Conformal Coating ◽  
Early Atmosphere ◽  
Living Organisms ◽  
Hypothetical Structure

In recent years major advances in surface chemistry and surface functionalization have been performed through the development, most often inspired by living organisms, of versatile methodologies. Among those, the contact of substrates with aminomalononitrile (AMN) containing solutions at pH = 8.5 allows a conformal coating to be deposited on the surface of all known classes of material. Since AMN is a molecule probably formed in the early atmosphere of our planet and since HCN-based compounds have been detected on many comets and Titan (Saturn’s largest moon) it is likely that such molecules will open a large avenue in surface functionalization mostly for bio-applications. This mini review describes the state of the art of AMN-based coatings from their deposition kinetics, composition, chemical reactivity, hypothetical structure to their first applications as biomaterials. Finally, the AMN-based versatile coatings are compared to other kinds of versatile coating based on catecholamines and polyphenols.

Influence of Nanoparticle Shape, Size, and Surface Functionalization on Cellular Uptake

2013 ◽  
Vol 13 (10) ◽  
pp. 6485-6498 ◽  
Author(s):  
Ningning Ma ◽  
Chao Ma ◽  
Chuanyan Li ◽  
Ting Wang ◽  
Yongjun Tang ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Surface Functionalization ◽  
Nanoparticle Shape

Peptide-nucleic acid nanostructures for transfection

2012 ◽  
Vol 3 (3) ◽  
pp. 283-293 ◽  
Author(s):  
Burkhard Bechinger
Keyword(s):  
Nucleic Acid ◽  
Physicochemical Properties ◽  
Cellular Uptake ◽  
Peptide Nucleic Acid ◽  
Endosomal Escape ◽  
Medical Applications ◽  
Specific Cell ◽  
Charge Composition ◽  
Cell Surfaces ◽  
Intracellular Targeting

AbstractTo use nucleic acids in biomedical research and medical applications, these highly hydrophilic macromolecules have to be transported through the organism, targeted to specific cell surfaces, and have to cross cellular barriers. To this end, nanosized transfection complexes have been designed and several of them have been successfully tested. Here, the different steps of the transfection process and the particular optimization protocols are reviewed, including the physicochemical properties of such vectors (size, charge, composition), protection in serum, cellular uptake, endosomal escape, and intracellular targeting. The transfection process has been subdivided into separate steps and here special emphasis is given to peptides that have been designed to optimize these steps individually. Finally, complex devices encompassing a multitude of beneficial functionalities for transfection have been developed.

Role of Surface Functionalization on Cellular Uptake of AuNPs Characterized by Computational Microscopy

2020 ◽  
Vol 124 (10) ◽  
pp. 1898-1908 ◽  
Author(s):  
Thodsaphon Lunnoo ◽  
Jirawat Assawakhajornsak ◽  
Sukhum Ruangchai ◽  
Theerapong Puangmali
Keyword(s):  
Cellular Uptake ◽  
Surface Functionalization
Sign in / Sign up

Export Citation Format

Share Document