A Dual Sensor Probe for the Sequential Detection of Copper(II) and Lactic Acid

<div> We report self assembling/aggregation properties of acyl-thiourea derivative, N-((6-methoxy-pyridin-2-yl)carbamothioyl)benzamide<a>(NG1) </a><a>[i1]</a> and the disaggregation induced emission which leads to its application as fluorescence and colorimetric probe for the sensitive detection of Cu2+. The microscopy analysis of NG1via SEM, and AFM reveal that it self-assembles to give fiber-like morphologies.Interestingly,NG1 also assembles to fluorescent fibers which show tunable emission properties. Addition of Cu2+to these fibers causedisruption/disaggregation of fibers and a golden yellow fluorescence is produced due to disaggregation induced emission enhancement (DIEE). The application of NG1 as selective sensor for copper was further assessed by UV visible and fluorescence spectroscopy. Limit of Detection(LOD) of Cu2+ with colorimetry was 2.5ppm while LOD of Cu2+ with fluorescence was as low as 0.1ppm. This yellow fluorescence is quenched after the addition of lactic acid and hence NG1 could potentially be used for the sequential detection of both Cu and lactate.Further, structural modification of the probe NG1 suggest crucial role of both pyridine and acyl-thiourea moiety in the binding of Cu2+. The experimental results of interaction of NG1 with Cu2+ and lactate were also validated theoretically by quantum chemical calculations based on density functional theory (DFT). Finally, we explore the ability of NG1for the sequential detection of Cu2+ and lactate in cells, which suggestsNG1 can be used effectively for the cellular imaging applications and to selectively sense Cu2+. To, the best of our knowledge,</div> this is the first report wherein a dual sensor for Cu2+and lactate ion is synthesized and it may in allpossibilities pave the way for the diagnosis of Cu2+associated disorders like Wilson’s disease and in the detection of elevated lactate levels which are associated with the wide range of pathologies likemitochondrial diseases, cerebral ischemia and cancer. <div> </div>

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A novel sensor for sequential and cellular detection of copper and lactic acid

10.26434/chemrxiv.8003507 ◽

2020 ◽

Author(s):

Nidhi Gour ◽

Sumit Kharbanda ◽

Deepak Kumar Pandey ◽

Dheeraj K Singh ◽

Dhiraj Bhatia ◽

...

Keyword(s):

Lactic Acid ◽

Density Functional ◽

Limit Of Detection ◽

Sequential Detection ◽

Yellow Fluorescence ◽

Self Assembling ◽

Golden Yellow ◽

Wide Range ◽

Dual Sensor ◽

Lactate Levels

<div> We report self assembling/aggregation properties of acyl-thiourea derivative, N-((6-methoxy-pyridin-2-yl)carbamothioyl)benzamide<a>(NG1) </a><a>[i1]</a> and the disaggregation induced emission which leads to its application as fluorescence and colorimetric probe for the sensitive detection of Cu2+. The microscopy analysis of NG1via SEM, and AFM reveal that it self-assembles to give fiber-like morphologies.Interestingly,NG1 also assembles to fluorescent fibers which show tunable emission properties. Addition of Cu2+to these fibers causedisruption/disaggregation of fibers and a golden yellow fluorescence is produced due to disaggregation induced emission enhancement (DIEE). The application of NG1 as selective sensor for copper was further assessed by UV visible and fluorescence spectroscopy. Limit of Detection(LOD) of Cu2+ with colorimetry was 2.5ppm while LOD of Cu2+ with fluorescence was as low as 0.1ppm. This yellow fluorescence is quenched after the addition of lactic acid and hence NG1 could potentially be used for the sequential detection of both Cu and lactate.Further, structural modification of the probe NG1 suggest crucial role of both pyridine and acyl-thiourea moiety in the binding of Cu2+. The experimental results of interaction of NG1 with Cu2+ and lactate were also validated theoretically by quantum chemical calculations based on density functional theory (DFT). Finally, we explore the ability of NG1for the sequential detection of Cu2+ and lactate in cells, which suggestsNG1 can be used effectively for the cellular imaging applications and to selectively sense Cu2+. To, the best of our knowledge,</div> this is the first report wherein a dual sensor for Cu2+and lactate ion is synthesized and it may in allpossibilities pave the way for the diagnosis of Cu2+associated disorders like Wilson’s disease and in the detection of elevated lactate levels which are associated with the wide range of pathologies likemitochondrial diseases, cerebral ischemia and cancer. <div> </div>

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A new disaggregation induced emission enhancement (DIEE)based probe for cellular detection of copper and lactate

10.26434/chemrxiv.8003507.v3 ◽

2019 ◽

Author(s):

Nidhi Gour ◽

chandra kanth P ◽

Sumit Kharbanda ◽

Deepak Kumar Pandey ◽

Manoj kumar pandey ◽

...

Keyword(s):

Density Functional ◽

Limit Of Detection ◽

Sequential Detection ◽

Yellow Fluorescence ◽

Self Assembling ◽

Emission Enhancement ◽

Golden Yellow ◽

Wide Range ◽

Dual Sensor ◽

Lactate Levels

<div> We report self assembling/aggregation properties of acyl-thiourea derivative, N-((6-methoxy-pyridin-2-yl)carbamothioyl)benzamide<a>(NG1) </a><a>[i1]</a> and the disaggregation induced emission which leads to its application as fluorescence and colorimetric probe for the sensitive detection of Cu2+. The microscopy analysis of NG1via SEM, and AFM reveal that it self-assembles to give fiber-like morphologies.Interestingly,NG1 also assembles to fluorescent fibers which show tunable emission properties. Addition of Cu2+to these fibers causedisruption/disaggregation of fibers and a golden yellow fluorescence is produced due to disaggregation induced emission enhancement (DIEE). The application of NG1 as selective sensor for copper was further assessed by UV visible and fluorescence spectroscopy. Limit of Detection(LOD) of Cu2+ with colorimetry was 2.5ppm while LOD of Cu2+ with fluorescence was as low as 0.1ppm. This yellow fluorescence is quenched after the addition of lactic acid and hence NG1 could potentially be used for the sequential detection of both Cu and lactate.Further, structural modification of the probe NG1 suggest crucial role of both pyridine and acyl-thiourea moiety in the binding of Cu2+. The experimental results of interaction of NG1 with Cu2+ and lactate were also validated theoretically by quantum chemical calculations based on density functional theory (DFT). Finally, we explore the ability of NG1for the sequential detection of Cu2+ and lactate in cells, which suggestsNG1 can be used effectively for the cellular imaging applications and to selectively sense Cu2+. To, the best of our knowledge,</div> this is the first report wherein a dual sensor for Cu2+and lactate ion is synthesized and it may in allpossibilities pave the way for the diagnosis of Cu2+associated disorders like Wilson’s disease and in the detection of elevated lactate levels which are associated with the wide range of pathologies likemitochondrial diseases, cerebral ischemia and cancer. <div> </div>

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A new disaggregation induced emission enhancement (DIEE)based probe for cellular detection of copper and lactate

10.26434/chemrxiv.8003507.v2 ◽

2019 ◽

Author(s):

Nidhi Gour ◽

Vivek Shinh Kshtriya ◽

Dheeraj K Singh ◽

chandra kanth P ◽

Dhiraj Bhatia

Keyword(s):

Density Functional ◽

Limit Of Detection ◽

Sequential Detection ◽

Yellow Fluorescence ◽

Self Assembling ◽

Emission Enhancement ◽

Golden Yellow ◽

Wide Range ◽

Dual Sensor ◽

Lactate Levels

<div> We report self assembling/aggregation properties of acyl-thiourea derivative, N-((6-methoxy-pyridin-2-yl)carbamothioyl)benzamide<a>(NG1) </a><a>[i1]</a> and the disaggregation induced emission which leads to its application as fluorescence and colorimetric probe for the sensitive detection of Cu2+. The microscopy analysis of NG1via SEM, and AFM reveal that it self-assembles to give fiber-like morphologies.Interestingly,NG1 also assembles to fluorescent fibers which show tunable emission properties. Addition of Cu2+to these fibers causedisruption/disaggregation of fibers and a golden yellow fluorescence is produced due to disaggregation induced emission enhancement (DIEE). The application of NG1 as selective sensor for copper was further assessed by UV visible and fluorescence spectroscopy. Limit of Detection(LOD) of Cu2+ with colorimetry was 2.5ppm while LOD of Cu2+ with fluorescence was as low as 0.1ppm. This yellow fluorescence is quenched after the addition of lactic acid and hence NG1 could potentially be used for the sequential detection of both Cu and lactate.Further, structural modification of the probe NG1 suggest crucial role of both pyridine and acyl-thiourea moiety in the binding of Cu2+. The experimental results of interaction of NG1 with Cu2+ and lactate were also validated theoretically by quantum chemical calculations based on density functional theory (DFT). Finally, we explore the ability of NG1for the sequential detection of Cu2+ and lactate in cells, which suggestsNG1 can be used effectively for the cellular imaging applications and to selectively sense Cu2+. To, the best of our knowledge,</div> this is the first report wherein a dual sensor for Cu2+and lactate ion is synthesized and it may in allpossibilities pave the way for the diagnosis of Cu2+associated disorders like Wilson’s disease and in the detection of elevated lactate levels which are associated with the wide range of pathologies likemitochondrial diseases, cerebral ischemia and cancer. <div> </div>

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Relationship between the Stereocomplex Crystallization Behavior and Mechanical Properties of PLLA/PDLA Blends

Polymers ◽

10.3390/polym13111851 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1851

Author(s):

Hye-Seon Park ◽

Chang-Kook Hong

Keyword(s):

Mechanical Properties ◽

Lactic Acid ◽

Crystallization Behavior ◽

Mechanical Analysis ◽

X Ray Diffraction ◽

Nucleation Agent ◽

Scanning Calorimetry ◽

Nucleation Sites ◽

Wide Range ◽

Degree Of Crystallization

Poly (l-lactic acid) (PLLA) is a promising biomedical polymer material with a wide range of applications. The diverse enantiomeric forms of PLLA provide great opportunities for thermal and mechanical enhancement through stereocomplex formation. The addition of poly (d-lactic acid) (PDLA) as a nucleation agent and the formation of stereocomplex crystallization (SC) have been proven to be an effective method to improve the crystallization and mechanical properties of the PLLA. In this study, PLLA was blended with different amounts of PDLA through a melt blending process and their properties were calculated. The effect of the PDLA on the crystallization behavior, thermal, and mechanical properties of PLLA were investigated systematically by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), polarized optical microscopy (POM), dynamic mechanical analysis (DMA), and tensile test. Based on our findings, SC formed easily when PDLA content was increased, and acts as nucleation sites. Both SC and homo crystals (HC) were observed in the PLLA/PDLA blends. As the content of PDLA increased, the degree of crystallization increased, and the mechanical strength also increased.

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Dual sensor measurement shows that temperature outperforms pH as an early sign of aerobic deterioration in maize silage

Scientific Reports ◽

10.1038/s41598-021-88082-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Guilin Shan ◽

Wolfgang Buescher ◽

Christian Maack ◽

André Lipski ◽

Ismail-Hakki Acir ◽

...

Keyword(s):

Lactic Acid ◽

Aerobic Metabolism ◽

Maize Silage ◽

Aerobic Stability ◽

Dual Sensor ◽

Ruminant Diets ◽

Incubation Temperatures ◽

Biological Additive ◽

Real Time Information ◽

First Time

AbstractHigh quality silage containing abundant lactic acid is a critical component of ruminant diets in many parts of the world. Silage deterioration, a result of aerobic metabolism (including utilization of lactic acid) during storage and feed-out, reduces the nutritional quality of the silage, and its acceptance by animals. In this study, we introduce a novel non-disruptive dual-sensor method that provides near real-time information on silage aerobic stability, and demonstrates for the first time that in situ silage temperature (Tsi) and pH are both associated with preservation of lactic acid. Aerobic deterioration was evaluated using two sources of maize silage, one treated with a biological additive, at incubation temperatures of 23 and 33 °C. Results showed a time delay between the rise of Tsi and that of pH following aerobic exposure at both incubation temperatures. A 11 to 25% loss of lactic acid occurred when Tsi reached 2 °C above ambient. In contrast, by the time the silage pH had exceeded its initial value by 0.5 units, over 60% of the lactic acid had been metabolized. Although pH is often used as a primary indicator of aerobic deterioration of maize silage, it is clear that Tsi was a more sensitive early indicator. However, the extent of the pH increase was an effective indicator of advanced spoilage and loss of lactic acid due to aerobic metabolism for maize silage.

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P0031AURINE METABOLOMICS: AN EMERGING TOOL FOR DISSECTING BIOLOGICAL ABERRATIONS UNDERLYING RENAL DYSFUNCTION IN BARDET-BIEDL SYNDROME

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfaa142.p0031a ◽

2020 ◽

Vol 35 (Supplement_3) ◽

Author(s):

Miriam Zacchia ◽

Emanuela Marchese ◽

Marianna Caterino ◽

Margherita Ruoppolo ◽

Giovambattista Capasso

Keyword(s):

Lactic Acid ◽

Kidney Disease ◽

Renal Disease ◽

Renal Dysfunction ◽

Metabolic Pathways ◽

Healthy Subjects ◽

Hexadecenoic Acid ◽

Kidney Dysfunction ◽

Bardet Biedl Syndrome ◽

Wide Range

Abstract Background and Aims Bardet Biedl Syndrome (BBS) is a rare genetic disorder characterized by a wide range of organ dysfunction, including kidney disease. The severity of renal dysfunction is highly variable in this setting, ranging from tubular defects to the end stage renal disease, with poor genotype-phenotype correlation. Proteomics and metabolomics are powerful tools able to contribute to the better understanding of molecular basis of disease conditions. Our previous studies demonstrated that the urinary proteomic pattern of BBS patients differed from that of healthy subjects, with a set of deregulated proteins including cell adhesion and extracellular matrix organization proteins (1). The present study aims to characterize urine metabolomic profile of BBS patients, in order to identify both 1) potential disease biomarkers and 2) aberrant metabolic pathways underlying renal disease Method To this end, in the pilot study urine samples have been collected from 14 adult BBS patients and have been compared with healthy volunteers, using an untargeted strategy. In the confirmation study, 24 BBS patients with wide range of kidney dysfunction have been enrolled, and additional control groups, besides healthy subjects, were included: 1) age-gender-matched chronic kidney disease patients by other causes and 2) obese individuals. Results Several metabolites were de-regulated in BBS patients compared with normal subjects (lactic acid, glycolic acid,3-Hydroxypropionic acid, pyruvic acid, 3-hydroxyisobutyric acid, 2-ethyl-3-hydroxy-propionic acid, succinic acid, fumaric acid, erythropentonic acid, 2-hydroxyglutaric acid, 4-hydroxyphenyllactic acid, 3,4-pyridinedicarboxylic acid, retinoic acid, 4-hydroxyphenylacetic acid, palmitic acid, 9-Hexadecenoic acid, oleic acid and 9-Octadecenoic acid). The clusterization performed by MetaboAnalyst tool, revealed a possible deregulation of different metabolic pathways, including glycolysis, TCA cycle, pyruvate metabolism, lipids biosynthesis and glutamate metabolism (p-value <0.01) (figure 1); some of these pathways were described as de-regulated in other ciliopathies (2). In the confirmation study (on-going studies) some metabolites, including lactic acid and intermediates of Krebs cycle, correlated with kidney dysfunction only in the BBS group. Conclusion These findings suggest that urine metabolomic fingerprint of BBS patients is different from that of healthy subjects and indicate a possible deregulation of several metabolic pathways; some urinary molecules correlated with kidney dysfunction only in BBS patients, suggesting the specificity of these results.

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The response of the kidney of the freshwater rainbow trout to true metabolic acidosis

Journal of Experimental Biology ◽

10.1242/jeb.84.1.227 ◽

1980 ◽

Vol 84 (1) ◽

pp. 227-244 ◽

Cited By ~ 2

Author(s):

K. A. Kobayashi ◽

C. M. Wood

Keyword(s):

Lactic Acid ◽

Metabolic Acidosis ◽

Blood Lactate ◽

Renal Excretion ◽

Total Acid ◽

Time Courses ◽

Lactate Levels ◽

Urinary Acid ◽

Proton Load ◽

Lactate Excretion

Infusion of lactic acid into the bloodstream of trout produced a short-lived depression of blood pH and a long-lasting elevation of blood lactate. The lactate injected was distributed in a volume of 198 ml/kg. Renal excretion of lactate anion and total acid increased by approximately equal amounts during the period of high blood lactate levels, but total renal loss over 72 h accounted for only 2% of the lactate load and 6% of the proton load. Comparable differences in the time courses of blood lactate and pH changes occurred when lactacidosis was induced endogenously by normocapnic hypoxia. The immediate response of the kidney was similar to that with lactic acid infusion, but there was a long-lasting (12–72 + h) elevation of urinary acid efflux that was not associated with lactate excretion. Following hypoxia, renal excretion over 72 h accounted for 1% of the estimated lactate load and 12–25% of the proton load. A renal lactate threshold of 4–10 muequiv/ml prevents significant urinary lactate excretion. The response of the trout kidney to true metabolic acidosis is similar to that of the mammalian kidney.

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Synthesis and Properties of Nitrogen-Doped Carbon Quantum Dots Using Lactic Acid as Carbon Source

Materials ◽

10.3390/ma15020466 ◽

2022 ◽

Vol 15 (2) ◽

pp. 466

Author(s):

Kaixin Chang ◽

Qianjin Zhu ◽

Liyan Qi ◽

Mingwei Guo ◽

Woming Gao ◽

...

Keyword(s):

Quantum Dots ◽

Lactic Acid ◽

Fluorescence Quenching ◽

Functional Groups ◽

Fluorescence Intensity ◽

Carbon Quantum Dots ◽

Nitrogen Doped ◽

Ph Values ◽

Wide Range ◽

Nitrogen Doped Carbon

Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized in a one-step hydrothermal technique utilizing L-lactic acid as that of the source of carbon and ethylenediamine as that of the source of nitrogen, and were characterized using dynamic light scattering, X-ray photoelectron spectroscopy ultraviolet-visible spectrum, Fourier-transformed infrared spectrum, high-resolution transmission electron microscopy, and fluorescence spectrum. The generated N-CQDs have a spherical structure and overall diameters ranging from 1–4 nm, and their surface comprises specific functional groups such as amino, carboxyl, and hydroxyl, resulting in greater water solubility and fluorescence. The quantum yield of N-CQDs (being 46%) is significantly higher than that of the CQDs synthesized from other biomass in literatures. Its fluorescence intensity is dependent on the excitation wavelength, and N-CQDs release blue light at 365 nm under ultraviolet light. The pH values may impact the protonation of N-CQDs surface functional groups and lead to significant fluorescence quenching of N-CQDs. Therefore, the fluorescence intensity of N-CQDs is the highest at pH 7.0, but it decreases with pH as pH values being either more than or less than pH 7.0. The N-CQDs exhibit high sensitivity to Fe3+ ions, for Fe3+ ions would decrease the fluorescence intensity of N-CQDs by 99.6%, and the influence of Fe3+ ions on N-CQDs fluorescence quenching is slightly affected by other metal ions. Moreover, the fluorescence quenching efficiency of Fe3+ ions displays an obvious linear relationship to Fe3+ concentrations in a wide range of concentrations (up to 200 µM) and with a detection limit of 1.89 µM. Therefore, the generated N-CQDs may be utilized as a robust fluorescence sensor for detecting pH and Fe3+ ions.

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Peculiarities of Carbohydrate Exchange in Patients with Generalized Periodontitis of Young Age Persons

Galician Medical Journal ◽

10.21802/gmj.2018.2.3 ◽

2018 ◽

Vol 25 (2) ◽

Author(s):

Halyna Kimak ◽

Halyna Melnychuk ◽

Hanna Ersteniuk

Keyword(s):

Lactic Acid ◽

Lactate Dehydrogenase ◽

Carbohydrate Metabolism ◽

Young Patients ◽

Young Age ◽

Lactate Dehydrogenase Activity ◽

Significant Difference ◽

Oral Liquid ◽

Lactate Levels ◽

Healthy Persons

There were studied 92 somatically healthy persons of young age (18-25 years old), with generalized periodontitis (GP) of initial-I degree of development, among them: 30 patients with chronic generalized periodontitis (CGP), who were included into group І; and 32 patients with exacerbation of the chronic generalized periodontitis (ECGP) – into group II; and 30 healthy patients. The carbohydrate metabolism indexes were studied, namely: the content of glucose, pyruvate (pyruvic acid) and lactate (lactic acid) and lactate dehydrogenase activity (LDG) in the oral liquid.We have determined that in the presence of CGP of the initial-I degree of development, and especially in its exacerbation, there is a significant increase of indicators of carbohydrate metabolism. In young patients with CGP indicators of glucose, pyruvate, lactate and LDG activity in the oral liquid increased by 2.0, 1.34, 1.58 and 1.37 (p<0.001, p<0.01) times respectively, and in case of ECGP they grew even more: by 2.71, 1.98, 1.76 and 2.07 (p<0.001) times, respectively.Among all the indicators, that characterize the carbohydrate metabolism, in addition to the level of pyruvate, in case of different course of GP a significant difference was revealed: in patients with ECGP compared with the data in CGP, glucose and lactate levels and LDG activity in the oral liquid were significantly higher - at 35.75% (p<0.05), 34.29% (p<0.01) and 36.59% (p<0.001) respectively.Detected violations of carbohydrate metabolism indeces in the oral liquid indicate the involvement of these processes in the pathogenesis of GP and the necessity of their correction.

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