scholarly journals Peculiarities of Carbohydrate Exchange in Patients with Generalized Periodontitis of Young Age Persons

2018 ◽  
Vol 25 (2) ◽  
Author(s):  
Halyna Kimak ◽  
Halyna Melnychuk ◽  
Hanna Ersteniuk
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Carbohydrate Metabolism ◽  
Young Patients ◽  
Young Age ◽  
Lactate Dehydrogenase Activity ◽  
Significant Difference ◽  
Oral Liquid ◽  
Lactate Levels ◽  
Healthy Persons

There were studied 92 somatically healthy persons of young age (18-25 years old), with generalized periodontitis (GP) of initial-I degree of development, among them: 30 patients with chronic generalized periodontitis (CGP), who were included into group І; and 32 patients with exacerbation of the chronic generalized periodontitis (ECGP) – into group II; and 30 healthy patients. The carbohydrate metabolism indexes were studied, namely: the content of glucose, pyruvate (pyruvic acid) and lactate (lactic acid) and lactate dehydrogenase activity (LDG) in the oral liquid.We have determined that in the presence of CGP of the initial-I degree of development, and especially in its exacerbation, there is a significant increase of indicators of carbohydrate metabolism. In young patients with CGP indicators of glucose, pyruvate, lactate and LDG activity in the oral liquid increased by 2.0, 1.34, 1.58 and 1.37 (p<0.001, p<0.01) times respectively, and in case of ECGP they grew even more: by 2.71, 1.98, 1.76 and 2.07 (p<0.001) times, respectively.Among all the indicators, that characterize the carbohydrate metabolism, in addition to the level of pyruvate, in case of different course of GP a significant difference was revealed: in patients with ECGP compared with the data in CGP, glucose and lactate levels and LDG activity in the oral liquid were significantly higher - at 35.75% (p<0.05), 34.29% (p<0.01) and 36.59% (p<0.001) respectively.Detected violations of carbohydrate metabolism indeces in the oral liquid indicate the involvement of these processes in the pathogenesis of GP and the necessity of their correction. 

scholarly journals Custodiol-MP for ex vivo lung perfusion – A comparison in a porcine model of donation after circulatory determination of death

2021 ◽  
pp. 039139882199066
Author(s):  
Katharina Kalka ◽  
Zoe Keldenich ◽  
Henning Carstens ◽  
Björn Walter ◽  
Ursula Rauen ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Functional Outcomes ◽  
Porcine Model ◽  
Ex Vivo ◽  
Lung Perfusion ◽  
Determination Of Death ◽  
Ex Vivo Lung Perfusion ◽  
Lactate Dehydrogenase Activity ◽  
Lactate Levels

Introduction: Ex vivo lung perfusion (EVLP) is an established technique to evaluate and eventually recondition lungs prior to transplantation. Custodiol-MP (C-MP) solution is a new solution, designed for clinical machine perfusion, that has been used for kidneys. The aim of this study was to compare the effects of EVLP with Custodiol-MP on lung functional outcomes to the gold standard of EVLP with Steen Solution™. Material and Methods: In a porcine EVLP model of DCDD (Donation after Circulatory Determination of Death), lungs were perfused with Steen Solution™ (SS, n = 7) or Custodiol-MP solution supplemented with 55 g/l albumin (C-MP, n = 8). Lungs were stored cold for 4 h in low potassium dextran solution and subsequently perfused ex vivo for 4 h. During EVLP pulmonary gas exchange, activities of lactate dehydrogenase (LDH) and alkaline phosphatase (AP) as well as levels of lactate in the perfusate were recorded hourly. Results: Oxygenation capacity differed significantly between groups (averaged over 4 h: SS 274 ± 178 mmHg; C-MP 284 ± 151 mmHg p = 0.025). Lactate dehydrogenase activities and lactate concentrations were significantly lower in Custodiol-MP perfused lungs. In a porcine model of DCDD with 4 h of EVLP the use of modified Custodiol-MP as perfusion solution was feasible. The use of C-MP showed at least comparable lung functional outcomes to the use of Steen SolutionTM. Furthermore C-MP perfusion resulted in significantly lower lactate dehydrogenase activity and lactate levels in the perfusate and higher oxygenation capacity.

scholarly journals Changes in blood counts, serum lactate dehydrogenase activity and haptoglobin level in malaria infected subjects in Nnewi, Anambra state, Nigeria

2020 ◽  
Vol 8 (7) ◽  
pp. 2509
Author(s):  
Nancy C. Ibeh ◽  
Enyioma A. Udeh ◽  
Clara I. Ajuba ◽  
Patrick O. Manafa
Keyword(s):  
Risk Factors ◽  
Lactate Dehydrogenase ◽  
Parasite Density ◽  
Kinetic Method ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Lactate Dehydrogenase Activity ◽  
Significant Difference ◽  
Haptoglobin Level ◽  
Associated Risk Factors

Background: Malaria is a mosquito-borne public health problem which alters the blood counts, haptoglobin level and serum lactate dehydrogenase (sLDH) activity of the infected individuals. Some of the alterations are associated risk factors in malaria pathology. This study aims at elucidating changes in blood counts, sLDH activity and haptoglobin level in malaria infected subjects seen in a Tertiary Health Institution in Nnewi, as search for associated risk factors in malaria pathology.Methods: This cross sectional study enrolled 270 age matched subjects between 18-65 years. The test group (200) who tested positive to P. falciparium was placed into two groups based on their parasite counts with cut-off of  ≥1000 parasites x 109/L. Group one (100) had counts above the cut-off and group two (100) below. The control (70) was aparasitemic. The demographic data were noted and 4mls of blood drawn. 2mls in K3EDTA was for FBC testing using Mythic 22 hematology analyzer, and remaining dispensed into plain tubes was for sLDH assay by kinetic method and haptoglobin by ELISA technique.Results: The HCT, Hb, RBC and Platelet count of test were progressively significantly lowered (p=0.001) compared to control, with an intra-significant difference among the 3 groups (p<0.05), also the parameters were found to have an inverse significant relationship (p=0.001) to the parasite counts. This trend was also seen with haptoglobin while reverse was the case with LDH activity which rather increased significantly (p = 0.000) at opposite direction as parasite density increases.Conclusions: This study show that the degree of intravascular haemolysis is directly influenced by the parasite density, this portends that high endemicity and perennial parasiteamia in the study area could cause chronic anaemia and thrombocytopenia in the population studied.

scholarly journals EVALUATION OF INFLUENCE OF THE UNITED COMPLEX AND SURGICAL TREATMENT ON SOME INDEXES OF CARBOHYDRATE EXCHANGE FOR PATIENTS ON GENERALIZED PERIODONTITIS І-ІІ DEGREE OF DEVELOPMENT

2021 ◽  
pp. 15-18
Author(s):  
H.B. Kimak ◽  
L.V. Tarnavska
Keyword(s):  
Lactic Acid ◽  
Surgical Treatment ◽  
Carbohydrate Metabolism ◽  
Inflammatory Process ◽  
Oral Fluid ◽  
Pathogenic Factor ◽  
Liquid Content ◽  
St John’S Wort ◽  
St John's Wort ◽  
Oral Liquid

The dynamics of the inflammatory process in the periodontium, its generalization and chronization are determined not only by the composition of the microflora, but also by the state of the protective reactions of the organism (the pro-atherogenic spectrum of lipids and the disturbance of the carbohydrate metabolism) and the reactivity of the immune system in response to the pathogenic factor. Purpose of the study. The study of changes in the parameters of carbohydrate metabolism in the oral fluid of patients with generalized periodontitis (GP), before and during the different terms after the complex and surgical treatment, was determined the relevance of the direction which was chosen by us. Materials and methods. Somatically healthy people were examined and treated: 30 patients with chronic generalized periodontitis – group І, 32 patients – with exacerbation of chronic generalized periodontitis – group II and 30 persons with a healthy parodontium. Indicators of carbohydrate metabolism were determined in the oral liquid: content of glucose, pyruvate (pyruvic acid) and lactate (lactic acid) and lactate dehydrogenase (LDG) activity before, immediately and after 6 and 12 months after the treatment. A significant increase of all studied by us carbohydrate metabolism parameters (p<0.001) was found, which confirms its imbalance. In order to regulate the revealed violations, initial periodontal therapy was prescribed; oral dishes with the solution of St. John’s wort, gum applications and application of the gel were developed by us on the basis of medicinal herbs (extract of Echinacea purpureum, tincture of Eleutherococcus and St. John’s wort) and sorbent, and inside – the herbal syrup “Immuno-tone” and surgical treatment. Conclusions. The regulation of the parameters of carbohydrate metabolism in the oral liquid of patients of groups I and II with the achievement of these standards, especially after 6 and 12 months after the treatment, showed the effectiveness of the developed by us therapeutic complex and surgical treatment allowed us to recommend it for the widespread introduction into practice.

scholarly journals Functional Replacement of the Escherichia colid-(−)-Lactate Dehydrogenase Gene (ldhA) with the l-(+)-Lactate Dehydrogenase Gene (ldhL) from Pediococcus acidilactici

2003 ◽  
Vol 69 (4) ◽  
pp. 2237-2244 ◽  
Author(s):  
Shengde Zhou ◽  
K. T. Shanmugam ◽  
L. O. Ingram
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Optical Purity ◽  
Pediococcus Acidilactici ◽  
Coding Region ◽  
Mineral Salts ◽  
Lactate Dehydrogenase Activity ◽  
Fermentation Products ◽  
Dehydrogenase Gene

ABSTRACT The microbial production of l-(+)-lactic acid is rapidly expanding to allow increased production of polylactic acid (PLA), a renewable, biodegradable plastic. The physical properties of PLA can be tailored for specific applications by controlling the ratio of l-(+) and d-(−) isomers. For most uses of PLA, the l-(+) isomer is more abundant. As an approach to reduce costs associated with biocatalysis (complex nutrients, antibiotics, aeration, product purification, and waste disposal), a recombinant derivative of Escherichia coli W3110 was developed that contains five chromosomal deletions (focA-pflB frdBC adhE ackA ldhA). This strain was constructed from a d-(−)-lactic acid-producing strain, SZ63 (focA-pflB frdBC adhE ackA), by replacing part of the chromosomal ldhA coding region with Pediococcus acidilactici ldhL encoding an l-lactate dehydrogenase. Although the initial strain (SZ79) grew and fermented poorly, a mutant (SZ85) was readily isolated by selecting for improved growth. SZ85 exhibited a 30-fold increase in l-lactate dehydrogenase activity in comparison to SZ79, functionally replacing the native d-lactate dehydrogenase activity. Sequencing revealed mutations in the upstream, coding, and terminator regions of ldhL in SZ85, which are presumed to be responsible for increased l-lactate dehydrogenase activity. SZ85 produced l-lactic acid in M9 mineral salts medium containing glucose or xylose with a yield of 93 to 95%, a purity of 98% (based on total fermentation products), and an optical purity greater than 99%. Unlike other recombinant biocatalysts for l-lactic acid, SZ85 remained prototrophic and is devoid of plasmids and antibiotic resistance genes.

Cocaine and exercise: alteration in carbohydrate metabolism in adrenodemedullated rats

1996 ◽  
Vol 80 (1) ◽  
pp. 124-132 ◽  
Author(s):  
E. O. Ojuka ◽  
J. D. Bell ◽  
G. W. Fellingham ◽  
R. K. Conlee
Keyword(s):  
Lactic Acid ◽  
Carbohydrate Metabolism ◽  
Adrenal Medulla ◽  
Muscle Glycogen ◽  
Combined Treatment ◽  
The Other ◽  
Plasma Lactate ◽  
Catecholamine Response ◽  
Lactate Levels

The combined treatment of cocaine-exercise (CE) causes an exaggerated catecholamine response, a rapid depletion of muscle glycogen, and accumulation of lactic acid. To assess the contribution of the adrenal medulla in the catecholamine response and to determine the role of epinephrine (Epi) on carbohydrate metabolism, cocaine (20 mg/kg ip) or saline was injected into sham-operated (Sham) or adrenodemedullated (AdM) rats, which then ran for 5 min at 56 m/min, 0% grade. In Sham rats, CE caused plasma Epi values (means +/- SE) to rise to 27.7 +/- 6.9 nM compared with 13.3 +/- 1.5 nM in saline-exercise (SE) and 0.8 +/- 0.2 nM in both AdM-CE and AdM-SE animals (P < 0.05). With minimal Epi in AdM, CE still caused glycogen to fall to lower levels (25.4 +/- 3.0 mumol/g vs. 40.5 +/- 2.4 mumol/g) and lactate to rise to higher levels (17 +/- 3 vs. 9 +/- 1 mumol/kg) in white vastus muscle than in SE group (P < 0.05). CE had no significant effect on soleus and red vastus glycogenolysis but it did cause lactate accumulation in red vastus. As a result, plasma lactate levels were also higher after CE compared with SE in AdM (17.9 +/- 2.0 vs. 8.5 +/- 0.5 mM, P < 0.05). We conclude that during CE 1) Epi is not essential to the alteration in carbohydrate metabolism; 2) the latter may be related to the other catecholamines; 3) the adrenal medulla is the only source of Epi; and 4) the adrenal medulla is not the source of the increased levels of norepinephrine or dopamine.

Construction and Potential Application of Controlled Autolytic Systems for Lactobacillus casei in Cheese Manufacture

2013 ◽  
Vol 76 (7) ◽  
pp. 1187-1193 ◽  
Author(s):  
YI XU ◽  
JIAN KONG
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Lactic Acid Bacteria ◽  
Enterococcus Faecalis ◽  
Lactobacillus Casei ◽  
Biological Process ◽  
Lactate Dehydrogenase Activity ◽  
Cheese Ripening ◽  
Rapid Release ◽  
Intracellular Enzymes

The rapid release of intracellular enzymes into the curd by the autolysis of lactic acid bacteria starters is universally recognized as a critical biological process to accelerate cheese ripening. Lactobacillus casei is typically the dominant nonstarter lactic acid bacterium in the ripening cheese. In this study, two controlled autolytic systems were established in L. casei BL23, based on the exploitation of the autolysins sourced from Lactococcus lactis (AcmA) and Enterococcus faecalis (AtlA). The lysis abilities of the systems were demonstrated both in broth and a model cheese, in which a fivefold increase in lactate dehydrogenase activity was detected in the curd with sufficient viable starter cells being maintained, indicating that they could lead to the timely release of intracellular enzymes.

scholarly journals EFFECT OF OLIGOSACCHARIDES ON ENZYMES OF CARBOHYDRATE METABOLISM AND ANTIOXIDANT PROTECTION IN IN VITRO TREATED ERYTHROCYTES UNDER CONDITIONS OF HYPERGLYCEMIA

2021 ◽  
Vol 27 (4) ◽  
pp. 4143-4150
Author(s):  
Ivica Dimov ◽  
◽  
Mariya Choneva ◽  
Ilia lliev ◽  
Anelia Bivolarska ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Glutathione Reductase ◽  
Carbohydrate Metabolism ◽  
Control Group ◽  
Antioxidant Protection ◽  
Antioxidant Power ◽  
Significant Difference ◽  
Ferric Reducing Antioxidant Power ◽  
Enzymes Of Carbohydrate Metabolism

Introduction: The purpose of this experiment is to examine the effect of different oligosaccharides with proven prebiotic effects on enzymes of carbohydrate metabolism and the antioxidant protection of erythrocytes in vitro under conditions of hyperglycemia. Materials and methods: This experiment included 10 healthy men (27±3 years of age). The isolated erythrocytes were treated with 1% and 5% solutions of the following oligosaccharides: lactulose, inulin, galactooligosaccharide and fructooligosaccharide in the presence of 5mM, 50mM and 100mM glucose. After incubation, for 2 hours at 37 °C, the erythrocytes were lysed, and the supernatant was used for analyses of lactate dehydrogenase, hexokinase and glutathione reductase. FRAP (Ferric reducing antioxidant power) method was used for determining the total antioxidant activity of erythrocytes. Results: Lactate dehydrogenase was decreased in the presence of 5% lactulose in groups with 50mM and 100 mM Glc. An increase in the activity of glutathione reductase under severe hyperglycemia (100mM glucose) was observed after treatment with: 1% lactulose, 1% inulin, 1% galactooligosaccharide, 1% and 5% fructooligosaccharides (p<0.005). A significant difference in the enzymatic activity of hexokinase was found in all groups (p<0.05) and of glutathione reductase only in the control group as well as in the groups treated with 1% lactulose, 1% galactooligosaccharide, 1% and 5% fructooligosaccharides Conclusions: Galactooligosaccharides 1% and fructooligosaccharides 1% and 5% cause a statistically significant increase of the enzymatic activities of hexokinase and glutathione reductase in in vitro hyperglycemia induced by 100 mM glucose, as well as an increase in FRAP.

scholarly journals A Study of Prognostic Factors in Young Patients With Non-HPV Oral Cancer in Central Europe

2021 ◽  
Vol 27 ◽  
Author(s):  
Katalin Csurgay ◽  
Attila Zalatnai ◽  
Márta Benczik ◽  
Benedek Krisztián Csomó ◽  
Ferenc Horváth ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Oral Cancer ◽  
Squamous Cell ◽  
Young Patients ◽  
Squamous Cell Carcinomas ◽  
Young Age ◽  
Etiological Factors ◽  
Significant Difference ◽  
Patient Groups ◽  
Immunohistochemical Profile

The etiological factors of squamous cell carcinomas of the head and neck have been well known for a long time. It is also well known that the incidence of oral cancer diagnosed in younger patients is on the rise. Due to the young age of these patients, the increase in the number of these cases and the fact that many of them neither smoke nor drink alcohol it has been suggested that other factors might be at play in the carcinogenesis of oral cancer. Thus, along the classic etiological factors of smoking and alcohol abuse certain molecular marker anomalies and the human papilloma virus (HPV) have emerged as potential factors. The aim of the present study is to verify the potential prognostic factors and to map the differences in biomarker expression between the young and the old patient groups. In the present study the immunohistochemical profile of samples obtained from oral squamous cell carcinomas was studied and compared with various clinico-pathological parameters. In 88 samples the expressions of p16, p53, Ki67, EGFR were studied with a tissue microarray technique under standard reaction conditions as well as the detection and typing of HPV infection with the Full Spectrum HPV DNA method. The biomarker expression profile of young patients with oral squamous cell carcinoma was compared to that of older patients (above 50). A significant difference was found between the immunohistochemical profile of the young and old patient groups in p16, Ki67 expression. The overall survival and progression free survival were influenced by p16 expression in young age.

The impact of young age on outcome in colon cancer

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 4075-4075
Author(s):  
C. Truong ◽  
S. Kempton ◽  
S. Lum ◽  
J. W. Morgan ◽  
J. H. Wong ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Young Patients ◽  
Stage Iv ◽  
Young Age ◽  
Rank Test ◽  
Primary Tumors ◽  
Old Patients ◽  
Significant Difference ◽  
The Mean ◽  
The Impact

4075 Background: The early age of diagnosis (<40 years) of colon cancer (CC) is generally considered to be associated with a particularly grave prognosis. Our objective was to determine the prognostic relevance of young age on outcome in CC. Methods: Retrospective cohort study from Region 5 of the California Cancer Registry (R5 CCR). Survival by Kaplan- Meier with significance assessed by log-rank test, T-test and Chi Square where appropriate. Results: Between January 1,1994 and December 31, 2003 10,730 patients with CC were diagnosed in R5 CCR. The patients ranged in age from 18–103 years (mean 71.1 years). Two hundred fourteen patients (2%) were 40 years of age or younger. The mean age of young patients was 34.6 years (range 18–40) and for old patients 71.3 years (range 41–103). The mean number of nodes examined were 18.5 in the young CC patient and 18.4 in the remainder of CC patients. There was no significant difference in the anatomic sub-site of the primary between younger and older CC patients (p=0.43). Young patients presented with more advanced primary tumors (T4 18.7% vs. 11.6%, p=0.03), more frequently with more extensive nodal involvement (N+ (54.4% vs. 40.2%, p<0.0001), and more frequently with distant metastatic disease (M1 25.7% vs. 18.3%, p=0.005). Despite these adverse characteristics, young patients had a significantly better disease specific survival (DSS) compared to their older counterparts (5 year survival 70.1% vs. 62.3%, p=0.02). Young patients had a suggestion of improved DSS compared to older patients in Stage I disease (5 yr DSS 96.0% vs. 90.5%, p=0.34) with more certain evidence of improved DSS in Stage II (5 yr DSS 94.8% vs. 79.9%, p=0.02), Stage III (5 yr DSS 73.4% vs. 57.2%, p=0.01) and most profoundly in Stage IV (5 yr DSS 20.1% vs. 0.08%, p=0.002). Conclusions: Despite adverse characteristics at diagnosis, young CC patients have a better DSS than their older counterparts. This is in contrast to the generally held opinion that young CC patients fare worse. This may reflect the ability of young CC patients to accept and tolerate more intense and aggressive therapies. No significant financial relationships to disclose.

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