scholarly journals The association between gene polymorphism of certain cytokines (IL-6, IL-10) and hepatitis C virus infection locally

2019 ◽  
Vol 10 (3) ◽  
pp. 2047-2053
Author(s):  
Najwan Abdul Karim Habib Naji Alzubaidi ◽  
Syoof Khoman Alwan
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Gene Polymorphism ◽  
Interleukin 10 ◽  
Vital Role ◽  
Polymerase Chain Reaction Test ◽  
Control Group ◽  
Blood Samples ◽  
Chain Reaction Test ◽  
And Control

Thirty blood samples were collected from people infected with HCV and collected 30 blood samples from healthy individuals as a control group. The samples were collected from the virus department at Diwaniyah Teaching Hospital, Women's Hospital and Educational Children and the Blood Bank in Diwaniyah Governorate, during the period from July 2018 until February 2019. Antibodies (Anti-HCV ) to HCV were detected in the infected serum using ELISA technique, and the diagnosis was confirmed Using the polymerase chain reaction test. Select gene polymorphism of interleukin -6 at site 174, which represents 3 genotypes GG, GC, CC in patients with HCV and control group. GG was significantly increased in patients with hepatitis C virus (63.3%) compared to the control group (30 %). The genotypes GC, CC were significantly decreased in patients with hepatitis C virus (16.7% and 20%) compared with the control group (43.3% and 26.7%), respectively. It also identified gene polymorphism of interleukin -10 at site 592, which represented 3 genotypes AA, AC, CC in patients with hepatitis C virus and control group. Genotype AC was significantly increased in patients with hepatitis C virus (53%) compared with the control group (30%). The genotypes CC, AA were significantly decreased in patients with hepatitis C virus (26.7% and 20%) compared with the control group (26.7% and 43.3%) respectively. Conclude from our current study that genotypes or alleles of IL-6 and IL-10 may play an essential role in increasing the risk of HCV in humans or play a vital role for prevention against.

scholarly journals Comparison of biomarkers between genotypes 1a and 3a in hepatitis C virus patients with control group

2019 ◽  
Vol 6 (4) ◽  
pp. 3121-3130
Author(s):  
Sajjad Yazdansetad ◽  
Hadi Razavi Nikoo ◽  
Seyed Mahmoud Azimi ◽  
Alireza Mohebbi ◽  
Massumeh Niazi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Biochemical Markers ◽  
Liver Enzymes ◽  
Control Group ◽  
Genotype 1A ◽  
Significant Difference ◽  
Hcv Genotyping ◽  
And Control ◽  
Genotype 3A

Introduction: Three percent of people worldwide are infected with Hepatitis C virus (HCV). A few studies have been performed to evaluate the biochemical markers of the disease. In the current study, biochemical markers were evaluated in HCV patients and the control group. Methods: Two sex- and age-matched healthy individuals (n = 100) and HCV positive patients (n = 100) were included (mean age of 20-75, 26.0% females and 74.0% males). Biochemical markers, including liver enzymes (ALT, AST and ALP), lipid profiles (cholesterol, LDL, and HDL) and triglyceride (TG) were investigated in both groups. HCV genotyping was also performed by Polymerase Chain Reaction (PCR) and OHNO methods. Results: The biochemical markers between HCV patients and controls were compared (cholesterol, ALP, AST, ALT, LDL: p = 0.0001, HDL: p = 0.002, TG: p = 0.003), and statistically significant difference was found between two groups. The biochemical markers between HCV patients and the control group in terms of age was compared and no differences was observed (p = 0.741), however, there was a significant difference in sex between HCV patients and control group (26.0% females, 74.0% males in control group, and x% females and y% males in HCV patients) (p = 0.032). The results of HCV genotyping showed that 39 patients were genotype 1a, and 43 patients were genotype 3a, and 1 patient was genotype 2a. Evaluation of biochemical markers in patients with genotype 1a and 3a showed that there were significant differences in cholesterol (p = 0.001), LDL (p = 0.001) and HDL (p= 0.003) levels, but there were no significant differences in liver enzymes and TG levels in both genotypes. Conclusion: In the present study, we found significant difference in biochemical markers between HCV patients and controls. In HCV patients, the biochemical markers were dependent on HCV genotypes, and their levels in genotype 1a were higher than genotype 3a. In conclusion, biochemical markers are one of the most important factors for the identification of treatment.

scholarly journals EVALUATION OF INTERLEUKIN-10 LEVELS IN THE PLASMA OF PATIENTS WITH VARIOUS STAGES OF TUBERCULOSIS

2015 ◽  
Vol 88 (2) ◽  
pp. 164-167 ◽  
Author(s):  
Pacifique Ndishimye ◽  
Fouad Seghrouchni ◽  
Bianka Domokos ◽  
Olga Soritau ◽  
Abderrahim Sadak ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Interleukin 10 ◽  
Vital Role ◽  
Control Group ◽  
Clear Understanding ◽  
X Rays ◽  
Abnormal Chest ◽  
Anti Inflammatory ◽  
Tuberculosis Therapy ◽  
And Control

Mycobacterium tuberculosis (Mtb) infection remains one of the world’s major causes of illness and mortality. A clear understanding of the host defense against Mtb is imperatively needed for the control of this epidemic. When tuberculosis (TB) infection occurs, a variety of pro and anti-inflammatory cytokines play a vital role in the pathogenesis of this disease. Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokines reported to suppress the protective immune response against tuberculosis.The aim of the present study was to evaluate the association of plasma IL-10 levels with various disease stages of TB and the possible effects of treatment on these levels. A group of 30 patients with active pulmonary TB and a control group of 21 healthy individuals were enrolled in this study. The levels of IL-10 were measured before, during, and after treatment using commercially available enzyme-linked immune-sorbent assay (ELISA). Data were analyzed using GraphPad Prism version 5.0.The results showed that the levels of IL-10 had significant differences between the TB and control groups (p<0.05). The patients with abnormal chest X-Ray findings had higher IL-10 levels when compared to patients with normal X-Rays (p=0.03). A subgroup of 18 patients were followed during the treatment and the mean plasma concentration of IL-10 in patients before therapy was higher than in patients at 3 months of therapy and in patients after 6 months of therapy (p=0.01). However, the IL-10 level remained significantly higher in patients at the end of treatment compared with controls.These findings could be used in follow-up as clinical biomarker of the success of tuberculosis therapy.

scholarly journals Salmonella Bacterin Vaccination Decreases Shedding and Colonization of Salmonella Typhimurium in Pigs

2021 ◽  
Vol 9 (6) ◽  
pp. 1163
Author(s):  
Eduarda Alexandra Gonçalves de Oliveira Moura ◽  
Daniela Gomes da Silva ◽  
Caio Henrique Turco ◽  
Thainara Vitoria Carnevalli Sanches ◽  
Gabriel Yuri Storino ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Blood Count ◽  
Control Strategies ◽  
Control Group ◽  
Blood Samples ◽  
Commercial Vaccine ◽  
Serum Igg ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Since the occurrence of swine salmonellosis has increased over time and control strategies other than biosecurity are highly recommended, the present study aimed to evaluate the efficacy of vaccination with Salmonella Choleraesuis and Salmonella Typhimurium bacterins in pigs. Two experimental groups were formed: G1, animals immunized with two doses of a commercial vaccine (n = 20); G2, control group (n = 20). After vaccination, all pigs were orally challenged (D0) with 108 CFU of Salmonella Typhimurium and evaluated for 40 days. Every 10 days after D0, five piglets from each experimental group were euthanized and submitted to the necroscopic examination, when organ samples were collected. Blood samples and rectal swabs were collected before the first dose of the vaccine (D−42), before the second dose (D−21), before the challenge (D0), and thereafter, every three days until D39. Blood count, serum IgG measurement by ELISA, and the excretion of Salmonella Typhimurium in feces were evaluated. While the results from blood count and serum IgG concentration did not differ, the detection and excretion of Salmonella between G1 and G2 differed (p < 0.05). Therefore, it was observed that this vaccine partially protected the animals against experimental infection with Salmonella Typhimurium, reducing the excretion of bacteria in feces.

Role of the specific T‐cell response for clearance and control of hepatitis C virus

1999 ◽  
Vol 6 (s1) ◽  
pp. 36-40 ◽  
Author(s):  
G. R. Pape ◽  
T. J. Gerlach ◽  
H. M. Diepolder ◽  
N. Grüner ◽  
M.‐C. Jung ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
T Cell ◽  
Cell Response ◽  
T Cell Response ◽  
And Control

IL‐28β gene polymorphism determines virological response to PEGylated interferon therapy in hepatitis C virus genotype 4 Egyptian patients

2018 ◽  
Vol 120 (5) ◽  
pp. 8154-8159 ◽  
Author(s):  
Nashwa El‐Khazragy ◽  
Naglaa El Sayed ◽  
Ahmed M. Salem ◽  
Nahla S. Hassan ◽  
Amal Tohamy Abdelmoeaz ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Gene Polymorphism ◽  
Virological Response ◽  
Interferon Therapy ◽  
Pegylated Interferon ◽  
Virus Genotype ◽  
Genotype 4 ◽  
Egyptian Patients
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