STUDY ON THE ANTIOXIDANT, ANTIBACTERIAL, AND CYTOTOXIC ACTIVITIES IN VITRO OF ROOT EXTRACTS FROM CODONOPSIS JAVANICA IN KON TUM PROVINCE, VIETNAM

Dangshen Codonopsis javanica performed high medicinal properties in herbal remedies; however, there is currently not much specific analysis for phytochemicals and bioactivities of this plant. In this study, the ethanol extract of C. javanica roots obtained from Kon Tum Province, Vietnam contained several substances as saponin, phenolic acid, terpenoid and alkaloid. The antibacterial effect of the extract showed against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus with the IC50 value of 150, 100, 150 and 90 μg/ml, respectively. The antioxidant capacity of the extract also displayed IC50 value of 46.8 ± 6.8 μg/ml. Moreover, the anticancer ability of the extract exhibited on two human cancer HepG2 cells (IC50 = 83.6 ± 2.7 μg/ml), and MCF-7 cells (IC50 = 95.3 ± 2.3 μg/ml). Hence, this study for the first time provide the basic data for further research of bioactivities of natural compounds Dangshen C. javanica.

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In Vitro Cytotoxic Effects of Secondary Metabolites Present in Sarcopoterium Spinosum L.

Applied Sciences ◽

10.3390/app11115300 ◽

2021 ◽

Vol 11 (11) ◽

pp. 5300

Author(s):

Jozef Hudec ◽

Jan Mojzis ◽

Marta Habanova ◽

Jorge A. Saraiva ◽

Pavel Hradil ◽

...

Keyword(s):

Mammary Gland ◽

Ethyl Acetate ◽

Cytotoxic Activity ◽

Cell Lines ◽

Benzalkonium Chloride ◽

Ethanol Extract ◽

Cytotoxic Activities ◽

Sarcopoterium Spinosum ◽

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Sarcopoterium spinosum (L.) is a medicinal plant traditionally used for the treatment of various diseases including cancer in the Near- and Middle East. The fractions and constituents of the ethanol extract of S. spinosum were screened for in vitro cytotoxic activities on Jurkat (acute T-lymphoblastic leukemia), HeLa (cervical adenocarcinoma), MCF-7 (mammary gland adenocarcinoma), Caco-2 (human colorectal adenocarcinoma), and MDA-MB-231 (mammary gland adenocarcinoma) cell lines using the MTT (3-(dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The ethanol extract was subsequently re-extracted with ethyl acetate and in its sub-fraction obtained by column chromatography three compounds (stachydrine, benzalkonium chloride and rutine) were the first time identified by nuclear magnetic resonance (NMR) analyses. The most active subfraction showed cytotoxic activity against HeLa, MCF-7, and Caco-2 cell lines. The three compounds mentioned, as standards of high-performance liquid chromatography (HPLC) quality, were studied individually and in combination. Cytotoxic activity observed might be due to the presence of benzalkonium chloride and rutin. Benzalkonium chloride showed the strongest growth suppression effect against HeLa cells (IC50 8.10−7 M) and MCF-7 cells (IC50 5.10−6 M). The mixture of stachydrine and benzalkonium chloride allowed a synergistic cytotoxic effect against all tested cancer and normal cells to be obtained. Anti-cancer activity of the plant extract of S. spinosum remains under-investigated, so this research describes how the three major compounds identified in the ethyl acetate extract can exert a significant dose dependent in vitro cytotoxicity.

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β-Dihydroagarofuran-type sesquiterpenoids from the stems of Celastrus orbiculatus with their cytotoxic activities

Letters in Organic Chemistry ◽

10.2174/1570178619666211220101500 ◽

2021 ◽

Vol 19 ◽

Author(s):

Yong Hua Lin ◽

Bao Yan Zhang ◽

Zhi Chao Chen ◽

Jian Feng Wei

Keyword(s):

Cytotoxic Activity ◽

Human Cancer ◽

Cytotoxic Activities ◽

Celastrus Orbiculatus ◽

Physico Chemical ◽

Ic50 Value ◽

Ic50 Values ◽

Hct 116 ◽

Hct 116 Cells

Abstract: A new β-dihydroagarofuran-type sesquiterpenoid named 1α,2α,5α,11-tetraacetoxy-8α-(trans-p-coumaroyl)-β-dihydroagarofuran (1), together with five known compounds (2-6) were isolated from the CHCl3-soluble extract of the stems of Celastrus orbiculatus. The structure of new compound was elucidated with spectroscopic physico-chemical analyses. All isolates were evaluated for in vitro cytotoxic activity against four human cancer lines including HepG2, MCF-7, A549 and HCT-116 cells. Among them, compounds 1 and 6 showed potent cytotoxic activities on HepG2 cells with IC50 values of 8.78 ± 2.31 and 10.28 ± 1.15 μM, respectively. In addition, compound 6 exhibited significant cytotoxic activity on HCT-116 cells with IC50 value of 6.37 ± 2.52 μM

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Two New Cytotoxic Steroidal Alkaloids from Sarcococca Hookeriana

Molecules ◽

10.3390/molecules24010011 ◽

2018 ◽

Vol 24 (1) ◽

pp. 11 ◽

Cited By ~ 1

Author(s):

Shaojie Huo ◽

Jichun Wu ◽

Xicheng He ◽

Lutai Pan ◽

Jiang Du

Keyword(s):

Cell Lines ◽

Human Cancer ◽

Cytotoxic Activities ◽

Steroidal Alkaloids ◽

X Ray ◽

Human Cancer Cell Lines ◽

X Ray Crystallography ◽

Ic50 Values ◽

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Two new steroidal alkaloids, named hookerianine A (1) and hookerianine B (2) were isolated from the stems and roots of Sarcococca hookeriana Baill., along with two known compounds, sarcorucinine G (3) and epipachysamine D (4). On the basis of spectroscopic methods and by comparison with literature data, their structures were determined. As well as X-ray crystallography was performed to confirm compound 4. To identify novel antitumor inhibitors, all compounds were performed a CCK-8 assay against five human cancer cell lines SW480, SMMC-7721, PC3, MCF-7 and K562 in vitro. Compound 2 exhibited moderate cytotoxic activities to all cell lines with IC50 values in the range of 5.97–19.44 μM. Compound 3 was the most effective one against SW480 and K562 cell lines with IC50 values of 5.77 and 6.29 μM, respectively.

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A New Xanthone from the Pericarp of Garcinia mangostana

Natural Product Communications ◽

10.1177/1934578x1300801219 ◽

2013 ◽

Vol 8 (12) ◽

pp. 1934578X1300801 ◽

Cited By ~ 2

Author(s):

Manqin Fu ◽

Samuel X. Qiu ◽

Yujuan Xu ◽

Jijun Wu ◽

Yulong Chen ◽

...

Keyword(s):

Colorimetric Assay ◽

Human Cancer ◽

Ethanol Extract ◽

2D Nmr ◽

Hep G2 ◽

Garcinia Mangostana ◽

Human Cancer Cell Lines ◽

Mtt Colorimetric Assay ◽

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A new prenylxanthone, garcimangostanol (1), was isolated from the EtOAc-soluble partition of the ethanol extract of the pericarp of Garcinia mangostana L., along with three known compounds, namely 8-deoxygartanin (2), 1-isomangostin (3), and garcinone C (4). The structure of compound 1 was elucidated on the basis of its 1D, 2D NMR and MS data. Compounds 1–4 exhibited either significant or moderate cytotoxicity against MCF-7, A549, Hep-G2 and CNE human cancer cell lines in vitro with IC50 values from 4.0 ± 0.3 to 23.6 ± 1.5 μM by MTT colorimetric assay.

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A New Optical Biflavonoid, (2″R)-2″,3″-Dihydrorobustaflavone 7,4′-Dimethyl Ether, and Other Constituents from Selaginella trichoclada Alsto

Natural Product Communications ◽

10.1177/1934578x19887885 ◽

2019 ◽

Vol 14 (12) ◽

pp. 1934578X1988788

Author(s):

Peng Yang ◽

Mei-Long Lu ◽

Ke Li ◽

Qun Zhou

Keyword(s):

Dimethyl Ether ◽

Spectroscopic Data ◽

High Performance ◽

Human Cancer ◽

Ethanol Extract ◽

Racemic Compound ◽

Chiral Phase ◽

Human Cancer Cell Lines ◽

First Time ◽

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A new optical biflavonoid, (2” R)-2″,3″-dihydrorobustaflavone 7,4′-dimethyl ether (1), and 6 known compounds (2-7) were isolated for the first time from the 70% ethanol extract of Selaginella trichoclada Alsto. The structures of the compounds were confirmed by extensive spectroscopic data analyses. Racemic compound 1 was separated by chiral-phase high-performance liquid chromatography, and the absolute configurations of (±)-1 were defined by circular dichroism spectroscopic data. Compound 1 exhibited moderate cytotoxicity against MCF-7, A549, and HepG2 human cancer cell lines.

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Ochroborbone, A New Cytotoxic Indole Alkaloid from Ochrosia borbonica

Natural Product Communications ◽

10.1177/1934578x1701200407 ◽

2017 ◽

Vol 12 (4) ◽

pp. 1934578X1701200

Author(s):

Yan-Ping Liu ◽

A-Hong Chen ◽

Ruo-Heng Li ◽

Hui-Wen Yang ◽

Hai-Nan Bao ◽

...

Keyword(s):

Human Cancer ◽

Indole Alkaloid ◽

Spectroscopic Methods ◽

Inhibitory Effects ◽

Human Cancer Cell Lines ◽

Stems And Leaves ◽

Monoterpenoid Indole Alkaloid ◽

First Time ◽

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A new monoterpenoid indole alkaloid, ochroborbone (1), along with five known alkaloids (2–6), were isolated from the stems and leaves of Ochrosia borbonica. Among them, ochroborbone (1) is a rare C17-nor monoterpenoid indole alkaloid, and the known compounds (2-6) were isolated from Ochrosia for the first time. These structures were established on the basis of extensive spectroscopic methods. All isolated compounds were evaluated for their cytotoxicities against five human cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7 and SW480 in vitro. Compounds 1 and 2 exhibited inhibitory effects with IC50 values comparable with those of cisplatin.

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Cytotoxicity of Kelakai (Stenochlaena palustris) Extract to MCF-7 Breast Cancer Cell

Jurnal Fitofarmaka Indonesia ◽

10.33096/jffi.v7i3.590 ◽

2020 ◽

Vol 7 (3) ◽

pp. 5-9

Author(s):

Harlyanti Muthma'innah Mashar ◽

Itma Annah

Keyword(s):

Breast Cancer ◽

Breast Cancer Cells ◽

Chemical Constituents ◽

Ethanol Extract ◽

Positive Control ◽

Cytotoxicity Assay ◽

Ic50 Value ◽

Mtt Reduction ◽

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Breast cancer is reported to rank fifth among all types of cancer with a case of death of 6.6%. In the Central Kalimantan region, early examinations of cancers including breast cancer were carried out and 247 tumors in the breast were identified (1.76%). Kelakai (Stenochlaena palustris) as one of the typical plants of Kalimantan which contain natural chemical constituents has been reported to be effective as an anti-inflammatory and antioxidant, so that with this potential can be developed to overcome diseases associated with it, one of them is breast cancer. This study aimed to examine the cytotoxicity of MCF-7 breast cancer cells using ethanol extract. The cytotoxicity assay of kelakai extract against MCF-7 cells conducted in vitro by the MTT reduction method. The variation of concentration used is 1000; 500; 250; 125; 62.5; 31.5; and 15.625 µg/ml, doxorubicin as a positive control was performed in a concentration of 1 μg / ml. The results of the cytotoxicity assay showed that the kelakai extract had a toxic effect on MCF-7 cells with an IC50 value of 493.57 µg / ml.

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Antiproliferative and anti-inflammatory acyl glucosyl flavones from the leaves of Bursera copallifera

Journal of the Mexican Chemical Society ◽

10.29356/jmcs.v62i4.624 ◽

2019 ◽

Vol 62 (4) ◽

Author(s):

María Crystal Columba-Palomares ◽

María Luisa Villarreal ◽

Silvia Marquina ◽

Antonio Romero-Estrada ◽

Verónica Rodríguez-López ◽

...

Keyword(s):

Human Cancer ◽

Folk Medicine ◽

Cancer Cell Line ◽

Traditional Use ◽

Ic50 Value ◽

Anti Inflammatory ◽

Phytochemical Components ◽

Cox 1 ◽

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Bursera copallifera(DC) Bullock (Burseraceae) is a plant species used in folk medicine for the treatment of inflammation (bronchitis, cough). The dichloromethane-methanol extract (DML) of B. copallifera leaves was found to possess important antiproliferative and anti-inflammatory activities. In order to further correlate these activities with the phytochemical components, the bioactivity-guided fractionation of the DML extract allowed the isolation of an active fraction which was constituted of five compounds characterized as as luteolin-3'-O-(3''-O-E-p-coumaroyl)-α-L-rhamnopyranoside (1), luteolin-3'-O-(2''-O-E-p-coumaroyl)-α-L-rhamnopyranoside (2), a-amyrin (3), 3-epilupeol (4) and stigmasterol (5). Among the five isolated constituents, the triterpenes 3 and 4, and the sterol 5 have been already well known by their anti-inflammatory properties. Flavone 2 (Luteolin-3'-O-(2''-O-E-p-coumaroyl)-α-L-rhamnopyranoside)was responsible for the antiproliferative activity, showing in vitro cytotoxic activity against MCF-7 human cancer cell line with IC50 value of 13.9 µM. By the other hand flavone (1) exhibited COX-1 inhibition (IC50 = 93 mM). Flavone 1, together with the triterpenes 3 and 4 were the responsible for inhibiting TPA-induced inflammation by inhibit COX-1 enzyme. Therefore, these results support the traditional use of the infusion made with the leaves of B. copallifera as an anti-inflammatory agent.

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SOME TRITERPENOIDS AND STEROIDS FROM THE LICHEN LOBARIA ORIENTALIS, LOBARIACEAE.

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/54/2c/11852 ◽

2018 ◽

Vol 54 (2C) ◽

pp. 313

Author(s):

Nguyen Thi My Dung

Keyword(s):

Spectroscopic Data ◽

Human Lung ◽

Ethyl Acetate Extract ◽

Cancer Cell Line ◽

Human Lung Cancer ◽

Cytotoxic Activities ◽

Ic50 Value ◽

Liver Hepatocellular Carcinoma ◽

First Time ◽

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Chemical investigation of the ethyl acetate extract of the lichen Lobaria orientalis,Lobariaceae, collected in Vietnam, afforded six known compounds, including four triterpenoids,zeorin (1), 16β-acetoxyhopane-6α,22-diol (2), retigeric acid A (3), retigeric acid B (4), togetherwith two steroids, ergosterol-5α,8α-peroxide (5) and cerevisterol (6). Their chemical structureswere elucidated by spectroscopic data analysis and comparison with those reported in theliterature. The cytotoxic activities of some compounds against liver hepatocellular carcinoma(HepG2), human lung cancer (NCI-H460), human epithelial carcinoma (HeLa) and humanbreast cancer (MCF-7) cell lines were evaluated. Compound 2 exhibited good cytotoxicityagainst MCF-7 cancer cell line with IC50 value of 21.10 μg/mL. This is the first time compounds(1), (2), (6) are reported in the Lobaria genus.

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