scholarly journals Cytotoxicity of Kelakai (Stenochlaena palustris) Extract to MCF-7 Breast Cancer Cell

2020 ◽  
Vol 7 (3) ◽  
pp. 5-9
Author(s):  
Harlyanti Muthma'innah Mashar ◽  
Itma Annah
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Cells ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Positive Control ◽  
Cytotoxicity Assay ◽  
Ic50 Value ◽  
Mtt Reduction ◽  
Mcf 7

Breast cancer is reported to rank fifth among all types of cancer with a case of death of 6.6%. In the Central Kalimantan region, early examinations of cancers including breast cancer were carried out and 247 tumors in the breast were identified (1.76%). Kelakai (Stenochlaena palustris) as one of the typical plants of Kalimantan which contain natural chemical constituents has been reported to be effective as an anti-inflammatory and antioxidant, so that with this potential can be developed to overcome diseases associated with it, one of them is breast cancer. This study aimed to examine the cytotoxicity of MCF-7 breast cancer cells using ethanol extract. The cytotoxicity assay of kelakai extract against MCF-7 cells conducted in vitro by the MTT reduction method. The variation of concentration used is 1000; 500; 250; 125; 62.5; 31.5; and 15.625 µg/ml, doxorubicin as a positive control was performed in a concentration of 1 μg / ml. The results of the cytotoxicity assay showed that the kelakai extract had a toxic effect on MCF-7 cells with an IC50 value of 493.57 µg / ml.

scholarly journals Cytotoxic Activity of BornUSU I towards T47D Breast Cancer Cells

2019 ◽  
Vol 7 (22) ◽  
pp. 3816-3818
Author(s):  
Nadiya Anandita Nasution ◽  
Urip Harahap ◽  
Ginda Haro ◽  
Hari Purnomo ◽  
Denny Satria
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Incubation Period ◽  
Breast Cancer Cells ◽  
In Vitro Study ◽  
Positive Control ◽  
Breast Cancer Treatment ◽  
Mtt Method ◽  
Ic50 Value

AIM: The aim of this study was to determine cytotoxic activity of BornUSU I or Boronhafagama I (1,5-bis(4-hydroxyphenyl)-3-oxa-1,5-diaza-2,4-diboropentane-2,4-diol) as a boron derivate compounds which are boron neutron captured theraphy (BNCT) candidates. METHODS: The T47D cells were treated by BornUSU I, and Tamoxifen as a positive control. The in vitro study was using MTT method with the incubation period for 24h and 48h. All data were determined using viability of cells equation for showing each IC50 value. RESULTS: The IC50 value of BornUSU I and Tamoxifen were 72.61 ± 0.82 µM and 10.62 ± 0.06 µM for 24 h incubation period, and for the 48 h incubation period were 44.63 ± 0.23 µM and 7.79 ± 0.05 µM. The 48 h incubation period results showed the lowest IC50 value. CONCLUSION: The results reveal that BornUSU I provide effective as anticancer, especially for breast cancer treatment.

scholarly journals Decreas of BCL-2 Expression by Ethanol Extract of Ocinum basilicum L. Leaves in Breast Cancer Cells

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Devi Nisa Hidayati ◽  
Fatimatuz Zahroh ◽  
Lina Wahyuni ◽  
Ibrahim Arifin
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Protein Expression ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Protein Expressions ◽  
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Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pathways that cause cell death can involve one of the proteins, which is BCL-2. This study aims to determine the decrease of BCL-2 protein expressions in breast cancer cells (T47D and MCF-7) tat are treated with the ethanol extract of Ocimum basilicum L. Ocimum basilicum L. was extracted using the maceration method with 70% ethanol solvent. The concentration of ethanol extract of Ocimum basilicum L. used to see the expression of BCL-2 protein in T47D and MCF-7 cells was 199 µg/ml and 388 µg / mL. The observation of BCL-2 protein expression is using immunocytochemical methods of T47D and MCF-7 cancer cells. The results showed that the ethanol extract of Ocimum basilicum L could reduce BCL-2 protein expression in breast cancer cells (T47D and MCF-7) at concentrations of 199 µg/ml and 388 µg/ml, respectively.

scholarly journals Ursolic Acid Enhances Doxorubicin Cytotoxicity on MCF-7 Cells Mediated by G2/M Arrest

2012 ◽  
Vol 3 (3) ◽  
pp. 410
Author(s):  
Ibrahim Arifin ◽  
Adam Hermawan ◽  
Muthi' Ikawati ◽  
Sari Haryanti ◽  
Anindyajati Anindyajati ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Ursolic Acid ◽  
Breast Cancer Cells ◽  
Chemotherapeutic Agent ◽  
Ic50 Value ◽  
Combinational Treatment ◽  
Mcf 7

Ursolic acid has been widely known to possess biological activity against numerous tumor cell lines. Previous studies revealed its cytotoxicity on several cancer cells in vitro by either inducing apoptosis or cell cycle modulation. This study was conducted to investigate ursolic acid’s cytotoxicity solely and in combination with a chemotherapeutic agent, doxorubicin, on MCF-7 breast cancer cells, followed by observation on its mechanism. Cytotoxicity of single and combinational treatment of ursolic acid and doxorubicin on MCF-7 breast cancer cells were conducted by using MTT assay. Single treatment was then evaluated by determining IC50 value, while combinational treatment was evaluated by analyzing cell viability and evaluating combination index (CI). To explore the mechanism underlying cytotoxic effect on respected cells, further analysis on cell cycle profile of single and combinational treatment was conducted by flow cytometry. Twenty four hours treatment of ursolic acid inhibited MCF-7 cells’ growth with IC50 value of 37 µM, while combinational treatment showed that several concentration combinations of ursolic acid and doxorubicin exhibited synergism of cytotoxic activity on MCF-7 cells, giving optimum CI value of 0.54. Flow cytometric analysis showed that combinational treatment induced G2/M arrest in MCF-7 cells. These results show that ursolic acid is promising to be developed as either single chemopreventive agent, or as doxorubicin’s co-chemotherapeutic agent in breast cancer treatment. Observation on the selectivity as part of safety aspect together with in silico, in vitro, and in vivo study on its molecular mechanism should be conducted.Keywords: ursolic acid, doxorubicin,co-chemotherapeutic agent, breast cancer, cell cycle

scholarly journals IC50 and Cell Viability of Combination of Ethanol Extract Moringa oleifera Leave (EEMo) and Ethanol Extract Carica papaya Leave (EECp) on Breast Cancer Cells

2020 ◽  
Vol 5 (01) ◽  
pp. 6-12
Author(s):  
Nuni Rismayanti Nurkalbi ◽  
Aryadi Arsyad ◽  
Ika Yustisia ◽  
Yulia Y Djabir
Keyword(s):  
Breast Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Moringa Oleifera ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Control Group ◽  
Ic50 Value ◽  
Mcf 7

This study aims to find out IC50 and Cell Viability of the combination of ethanol extract leaves of Moringa oleifera (EEMo) and ethanol extract leaves of Carica papaya (EECp) on the growth of MCF-7 breast cancer cells culture. It was conducted in a true experimental laboratory using post-test only control group design method. The study showed that the effect of extract combination for MCF-7 Cell by using series concentration like 0, 20, 40, 80 and 160 µg/mL for 48, 72, and 96 hours with a cell density of 5x103 after giving WST assay there is a decrease in the number of cell viability. Inhibition concentration of MCF-7 cell culture was also indicated by the IC50 value which was included in the very strong category with details of each extract combination with 1:1 comparison the IC50 value is 12.02 µg/mL. Keywords: extract combination; IC50; cell viability, MCF-7

scholarly journals Evaluation the effect of several anticancer drugs on Vietnamese breast cancer cells

2018 ◽  
Vol 21 (2) ◽  
pp. 44-51
Author(s):  
Oanh Thi-Kieu Nguyen
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Breast Cancer Cells ◽  
Multidrug Resistant ◽  
Positive Control ◽  
Breast Cancer Patients ◽  
Ic50 Value ◽  
Very High ◽  
Mcf 7

In Viet Nam, data from Conference of Cancer organized by the Ministry of Health has shown that breast cancer is the most popular cancer in women. Current mainly treatments are surgery, chemotherapy, and radiotherapy. However, the rate of recurrence after five years was very high. One of the causes of high relapse is cancer cells develop multidrug-resistant (MDR) thus reduced the efficiency of treatments. In this research, MTT assay was used for measured cell viability of Vietnamese breast cancer cells (VNBRCA cells) and positive control MCF-7 cell lines after treatment with several anticancer drugs as Doxorubicin (DOX), Tamoxifen (TAM), Mitomycin C (MMC) in 48h. After that, cancer cells were treated at haft maximal inhibitory concentration (IC50) of anticancer drug and observed cell morphology, apoptosis of cellular nuclear by AO/PI staining. IC50 value of VNBRCA cells with DOX, TAM, MMC were 0.641± 0.07 µM, 4.639 ± 0.933 µM and 1.338 ± 0.176 µM, respectively, which higher than IC50 of MCF-7 with DOX, TAM, MMC was 0.168 ± 0.037 µM, 7.085 ± 0.87 µM and 0.379 ± 0.159 µM, respectively. The response of VNBRCA cells with several anticancer drugs as DOX, TAM, and MMC was lower than the response of MCF-7, therefore, it showed that the specific features of VNBRCA cells; from which develop specific treatments for Vietnamese breast cancer patients.

Anticancer Potential of Calli Versus Seedling Extracts Derived from Rosmarinus officinalis and Coleus hybridus

2020 ◽  
Vol 21 (14) ◽  
pp. 1528-1538
Author(s):  
Sarah Albogami ◽  
Hadeer Darwish ◽  
Hala M. Abdelmigid ◽  
Saqer Alotaibi ◽  
Ahmed Nour El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Transcriptional Profiling ◽  
Significant Inhibition ◽  
Rosmarinus Officinalis ◽  
Crude Extracts ◽  
Incidence And Mortality ◽  
Mcf 7

Background: In Saudi Arabia, the incidence and mortality rates of breast cancer are high. Although current treatments are effective, breast cancer cells develop resistance to these treatments. Numerous studies have demonstrated that active compounds in plant extracts, such as the phenolic compound Rosmarinic Acid (RA), exert anti-cancer effects. Objective: We investigated the anticancer properties of methanolic crude extracts of seedlings and calli of Rosmarinus officinalis and Coleus hybridus, two Lamiaceae species. Methods: MCF-7 human breast cancer cells were treated with methanolic crude extracts obtained from plant calli and seedlings generated in vitro, and cell proliferation was evaluated. Transcriptional profiling of the seedling and callus tissues was also conducted. Results: The mRNA expression levels of RA genes were higher in C. hybridus seedlings than in R. officinalis seedlings, as well as in C. hybridus calli than in R. officinalis calli, except for TAT and C4H. In addition, seedling and callus extracts of both R. officinalis and C. hybridus showed anti-proliferative effects against MCF-7 cells after 24 or 48 h of treatment. Discussion: At a low concentration of 10 μg/mL, C. hybridus calli and seedling extracts showed the most significant anti-proliferative effects after 24 and 48 h of exposure (p < 0.01); controls (doxorubicin) also showed significant inhibition, but lesser than that observed with C. hybridus (p < 0.05). Results with R. officinalis callus and seedling extracts did not significantly differ from those with untreated cells. Conclusion: Methanolic extracts of R. officinalis and C. hybridus are potentially valuable options for breast cancer treatment.

In vitro evaluation of estrogenic, antiestrogenic and antitumor effects of amentoflavone

2021 ◽  
pp. 096032712199945
Author(s):  
AT Aliyev ◽  
S Ozcan-Sezer ◽  
A Akdemir ◽  
H Gurer-Orhan
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Antitumor Effects ◽  
Antiestrogenic Activity ◽  
Er Positive ◽  
In Vivo Effects ◽  
Mcf 7

Apigenin, a flavonoid, is reported to act as an estrogen receptor (ER) agonist and inhibit aromatase enzyme. However, amentoflavone, a biflavonoid bearing two apigenin molecules, has not been evaluated for its endocrine modulatory effects. Besides, it is highly consumed by young people to build muscles, enhance mood and lose weight. In the present study, apigenin was used as a reference molecule and ER mediated as well as ER-independent estrogenic/antiestrogenic activity of amentoflavone was investigated. Antitumor activity of amentoflavone was also investigated in both ER positive (MCF-7 BUS) and triple-negative (MDA-MB-231) breast cancer cells and its cytotoxicity was evaluated in human breast epithelial cells (MCF-10A). Our data confirmed ER agonist, aromatase inhibitory and cytotoxic effects of apigenin in breast cancer cells, where no ER mediated estrogenic effect and physiologically irrelevant, slight, aromatase inhibition was found for amentoflavone. Although selective cytotoxicity of amentoflavone was found in MCF-7 BUS cells, it does not seem to be an alternative to the present cytotoxic drugs. Therefore, neither an adverse effect, mediated by an estrogenic/antiestrogenic effect of amentoflavone nor a therapeutical benefit would be expected from amentoflavone. Further studies could be performed to investigate its in vivo effects.

scholarly journals Inhibition of MCF-7 breast cancer cell proliferation by 5alpha-dihydrotestosterone; a role for p21(Cip1/Waf1)

2004 ◽  
Vol 32 (3) ◽  
pp. 793-810 ◽  
Author(s):  
MA Greeve ◽  
RK Allan ◽  
JM Harvey ◽  
JM Bentel
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
S Phase ◽  
Cyclin D3 ◽  
P27 Kip1 ◽  
Mcf 7

Androgens inhibit the growth of breast cancer cells in vitro and in vivo by mechanisms that remain poorly defined. In this study, treatment of asynchronously growing MCF-7 breast cancer cells with the androgen, 5alpha-dihydrotestosterone (DHT), was shown to inhibit cell proliferation and induce moderate increases in the proportion of G1 phase cells. Consistent with targeting the G1-S phase transition, DHT pretreatment of MCF-7 cultures impeded the serum-induced progression of G1-arrested cells into S phase and reduced the kinase activities of cyclin-dependent kinase (Cdk)4 and Cdk2 to less than 50% of controls within 3 days. DHT treatment was associated with greater than twofold increases in the levels of the Cdk inhibitor, p27(Kip1), while p21(Cip1/Waf1) protein levels remained unchanged. During the first 24 h of DHT treatment, levels of Cdk4-associated p21(Cip1/Waf1) and p27(Kip1) were reduced coinciding with decreased levels of Cdk4-associated cyclin D3. In contrast, DHT treatment caused increased accumulation of Cdk2-associated p21(Cip1/Waf1), with no significant alterations in levels of p27(Kip1) bound to Cdk2 complexes. These findings suggest that DHT reverses the Cdk4-mediated titration of p21(Cip1/Waf1) and p27(Kip1) away from Cdk2 complexes, and that the increased association of p21(Cip1/Waf1) with Cdk2 complexes in part mediates the androgen-induced growth inhibition of breast cancer cells.

scholarly journals The Synthesis of Cinchonine Tiglat Ester Compound and Cytotoxic Test Against MCF-7 Breast Cancer Cell

2018 ◽  
Vol 19 (2) ◽  
pp. 54-61
Author(s):  
Ahmad Khanifudin ◽  
Gian Primahana ◽  
Sylvia Rizky Prima ◽  
Puspa Dewi Lotulung ◽  
Muhammad Hanafi
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cinchona Alkaloid ◽  
Alamar Blue ◽  
Tiglic Acid ◽  
Spectroscopy Instrumentation ◽  
Ic50 Value ◽  
Pass Through ◽  
Mcf 7

Cinchonine is a type of cinchona alkaloid compound commonly found and/or isolated from Cinchona sp. plant. It is commonly used to treat malaria, and can potentially be used against cancer cells. In this particular study, cinchonine ester derivatives were extracted through esterification processs. Synthesized esther is aimed to gain higher lipophilicity of cinchonine so that makes it easier to pass through cell membrane. Esterification was done using DCC activator as well as DMAP catalyst with tiglic acid used to create cinchonine tiglat. Subsequent cinchonine tiglat was obtained in the form of oil with 25,28% yield. The compound obtained from the synthesis was the analyzed using LC-ESI-MS and 1H-NMR spectroscopy instrumentation. Results show that the target compound has been successfully synthesized. Its cytotoxic ability against MCF-7 breast cancer cells was tested using the Alamar Blue method. Results concluded that cinchonine tiglat ester compound has a viable cytotoxic activity with IC50 value of 1.22 ppm.

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