DEVELOPMENT OF A METHOD FOR QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN TRIPLEUROSPERMUM INODORUM’S FLOWERS

2021 ◽  
pp. 157-166
Author(s):  
Blinova O.L. ◽  
Gileva A.A. ◽  
Hlebnikov A.V. ◽  
Belonogova V.D. ◽  
Turyshev A.Y.
Keyword(s):  
Quantitative Determination ◽  
Aluminum Chloride ◽  
Chloride Solution ◽  
Uv Spectra ◽  
Raw Material ◽  
Spectral Studies ◽  
Scientific Medicine ◽  
Differential Spectrophotometry ◽  
The Stability

Chamomilla recutita is used in scientific medicine. Tripleurospermum inodorum (L.) Sch. Bip. is widely spread among possible impurities of Chamomilla recutita (L.) Rauschert. This plant is perspective for establishment into scientific medicine but it can’t change Chamomilla recutita (L.) Rauschert. Purpose of research: development of a method for the quantitative determination of the amount of flavonoids in terms of rutin in Tripleurospermum inodorum’s flowers using differential spectrophotometry. Materials and methods. The samples prepared in different parts of Russia were used as objects of research. (2017 – 2020). Spectral studies were carried out in the wavelength range of 350-430 nm with a step of 1 nm using a spectrophotometer SF-2000. Results. To determine the analytical wavelength, the UV spectra of alcohol extraction of Tripleurospermum inodorum’s flowers were studied. Maximum of absorption was noticed at wavelength 370 nm. The differential spectrum of the same extraction with an aluminum chloride solution of 2% in 96% alcohol has a maximum at a length of 410±2 nm, which coincides with the maximum of the Standard Sample (SS) of rutin. The largest number of flavonoids is extracted by 70% alcohol. The maximum optical density and the highest output of the number of flavonoids from the raw material is observed at a degree of grinding of 2 mm with a single extraction for 60 minutes. In the conditions of complexation, the optimal ratio of the volume of the test solution and aluminum chloride with a solution of 2% in 96% alcohol is the ratio of 1:1. The stability of the complex with an aluminum chloride solution of 2% in 96% alcohol is observed in 40 minutes after the start of the reaction and retains it for 30 minutes. Findings. The method is developed and the parameters of the quantitative determination of the amount of flavonoids in Tripleurospermum inodorum flores are determined in terms of rutin using differential spectrophotometry.

scholarly journals QUANTITATIVE DETERMINATION OF FLAVONOIDS IN LAVATERA THURINGIACA L., GROWING IN ALTAI KRAI TERRITORY

2018 ◽  
Vol 35 (1) ◽  
pp. 95-101
Author(s):  
L M Fedoseeva ◽  
O A Myznikova
Keyword(s):  
Quantitative Determination ◽  
Raw Material ◽  
Maximum Accumulation ◽  
Quantitative Content ◽  
Differential Spectrophotometry

Aim. To determine the quantitative content of flavonoids in Lavatera thuringiaca L., the grass, growing in Altai Krai. Materials and methods. The object of the study: Lavatera thuringiaca L. grass, stored at different phases of vegetation in Altai Krai territory. Quantitative determination of flavonoids in the investigated raw material was fulfilled, using differential spectrophotometry method. Results. Maximum accumulation of flavonoids in Lavatera thuringiaca L. grass was found to occur in the period of budding (1.14 %) and blossoming (1.02 %). The sum of flavonoids in terms of rutin, extracted with 70 % ethanol from raw material, stored in different regions, is changed insignificantly and makes 1.00-1.23 %. Conclusions. The results of this work testify expediency of studying Lavatera thuringiaca L. as a source of flavonoids.

scholarly journals JUSTIFICATION OF THE METHODS FOR THE QUALITATIVE AND QUANTITATIVE DETERMINATION OF FLA-VONOIDS IN THE GRASS OF SPECIES OF THE GENUS MONARDA

2020 ◽  
pp. 159-169
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Anastasiya Sergeyevna Lapina
Keyword(s):  
Quantitative Determination ◽  
Aluminum Chloride ◽  
Absorption Maximum ◽  
Bathochromic Shift ◽  
Biologically Active ◽  
Raw Material ◽  
Chromatographic Study ◽  
Monarda Fistulosa ◽  
Promising Source

The herb of plants of the genus Monarda L. is most often known for its essential oil the main components of which are thymol and carvacrol. However flavonoids contained in the raw materials of this plant are of great interest. The literature reports on the presence in the herb of species of the genus Monarda L. quercetin, luteolin, rutin, hesperidin, naringinin; howeve, these data are contradictory. It is known that when determining the content of the amount of flavonoids, recalculation is carried out in different cases for luteolin or rutin, which have different spectrum characteristics. This paper discusses the results of a comparative study of the flavonoid composition of some species of the genus Monarda: Monarda fistulosa L., Monarda didyma L., Monarda bradburiana L. Back., Monarda citriodora L., Monarda × medioides W.H.Duncan as a promising source of biologically active compounds. As a result of a comparative chromatographic study, the presence of flavonoids was detected upon detection at a wavelength of 366 nm and after treatment with an alcohol solution of aluminum chloride. In all treatments, the presence of cynaroside, rutin, quercetin, luteolin is not confirmed. Using column chromatography on silica gel L 40/100, from the herb of Monarda fistulosa the flavonoid substances for the first time were isolated – isorhoifolin (7-O-rutinoside of apigenin) and linarin (7-O-rutinoside of acacetin), which have n-butanol–acetic acid–water (4 : 1 : 2) in the solvent system Rf values are about 0.5 and 0.6, respectively, and also dimydin (7-O-rutinoside of sakuranetin). It was determined that in all UV spectra of extracts from herb of studied species of the genus Monarda of the species, a bathochromic shift of the long-wavelength band is observed in the presence of 3% alcoholic solution aluminum chloride, which confirms the presence of flavonoids. Under the conditions of differential spectrophotometry, an absorption maximum is observed in the region of 392–396 nm, which indicates the feasibility of using isorhoifolin in the analysis technique with an absorption maximum at a wavelength of 394 nm. As a result of the work a method of quantitative determination of the amount of flavonoids of the herb of the Monarda has been developed. The optimal parameters were determined: 60% ethyl alcohol, the ratio of "raw material to extractant" – 1 : 50, extraction time – 60 minutes, analytical wavelength at 394 nm. It was determined that the content of the total of flavonoids calculated on isorhoifolin in all samples studied varies from 4.53% to 8.73%.

The issue of "oil shrinkage" during the compounding of oils in the processes of production, collection, preparation and transportation of hydrocarbon raw materials

2021 ◽  
pp. 104-113
Author(s):  
O. P. Deryugina ◽  
E. A. Trapeznikov
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Colloidal System ◽  
Main Pipelines ◽  
Different Types ◽  
Preliminary Study ◽  
The Stability ◽  
Mixed Oil

The article discusses the issue of industrial compounding, which improves the quality of the raw materials supplied to the main pipelines. Compounding from "to compound" (English) - to mix. When mixing different types of oil obtained, "incompatibility" is possible, which is expressed in the precipitation of a solid sediment and violation of the stability of the colloidal system and due to the differing properties of oils. Attention to this problem is due to the tightening of requirements for the quality of raw materials that must comply with modern standards. The article discusses the causes of the problem of "incompatibility" of oil during compounding and effective ways of solving it, substantiates the need for a preliminary study of the properties of mixed oil in order to identify possible incompatibility of various types of oil. The standard tests for determination of incompatibility indices characterizing the ability of raw materials to mix are considered. The article substantiates the need to develop methods for diagnosing the incompatibility of oils as the most important task of modern chemical science, the solution of which will improve the quality of the raw material obtained and solve many technological problems in the compounding process.

Thin Layer Chromatographic Method for Determination of Deoxynivalenol in Wheat: Collaborative Study

1986 ◽  
Vol 69 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Robert M Eppley ◽  
Mary W Trucksess ◽  
Stanley Nesheim ◽  
Charles W Thorpe ◽  
Albert E Pohland ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aluminum Chloride ◽  
Chloride Solution ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Sample Extraction ◽  
Layer Chromatography

Abstract A collaborative study of a rapid method for the determination of deoxynivalenol (DON) in winter wheat was successfully completed. The method involves sample extraction with acetonitrile-water (84 + 16), cleanup using a disposable column of charcoal, Celite, and alumina, and detection by thin layer chromatography after spraying with an aluminum chloride solution. Each of the 15 collaborators analyzed 12 samples, 2 of which were naturally contaminated, and 10 to which DON was added, in duplicate, at levels of 0,50,100,300, and 1000 ng/ g. Average recoveries of DON ranged from 78 to 96% with repeatabilities of 30-64% and reproducibilities of 33-87%. The results of the study show that false positives were not a problem and that all of the analysts could detect DON at the 300 ng/g level or higher. The method has been adopted official first action.

Infrared and Raman spectral studies of KNO2–KNO3 solutions

1968 ◽  
Vol 46 (2) ◽  
pp. 229-233 ◽  
Author(s):  
M. H. Brooker ◽  
D. E. Irish
Keyword(s):  
Quantitative Determination ◽  
Raman Band ◽  
Vibrational Frequencies ◽  
Spectral Studies ◽  
Vibration Spectroscopy ◽  
Potassium Nitrite ◽  
Raman Spectral

Potassium nitrite frequently contains significant nitrate impurity, detectable by vibration spectroscopy. Analyses based independently on infrared and Raman band intensities permit a quantitative determination of the nitrate impurity. Reassignment of some previously reported vibrational frequencies is discussed.

scholarly journals Research in the Development and Validation of the Method of Quantitative Determination of Flavonoids in the Common Sorrel Herba, Gathered in the Altai Territory

2019 ◽  
Vol 8 (2) ◽  
pp. 80-86 ◽  
Author(s):  
G. R. Kutateladze ◽  
L. M. Fedoseeva
Keyword(s):  
Particle Size ◽  
Aluminum Chloride ◽  
Raw Materials ◽  
Alcohol Solution ◽  
Raw Material ◽  
Complexation Reaction ◽  
Optimal Parameters ◽  
Quantitative Content ◽  
Development And Validation

Introduction. Common sorrel (Rumex acetosa L.) is a biennial herb from the Buckwheat family (Polygonaceae L.). In our previously studies of phenolic compounds composition of alcohol extraction and ethereal, ethyl acetate, butanol fractions of common sorrel herba and flavonoids of the flavonol group was found. From the literature sources it is known that flavonoids are one of the groups of biologically active compounds with anti-inflammatory action. In this regard, the actual task is to assess the quantitative content of flavonoids in common sorrel herba.Aim. Is the development and validation of a method for the quantitative determination of flavonoids in common sorrel herba.Materials and methods. It has been established that the flavonoid complex of common sorrel herba includes rutin, therefore, the reference sample (RS) rutin was used as a standard for calculating the amount of flavonoids. Alcohol extract of common sorrel herba and a solution of rutin RS were prepared. The complexation reaction with aluminum chloride was carried out. The resulting solutions were investigated by the method of differential spectrophotometry. The spectral characteristics of the test and standard samples were compared. Then we studied the effect of extraction conditions on the yield of flavonoids from the raw material: the extractant, the particle size of the raw material, the ratio of «raw material – extractant», temperature, frequency and duration of extraction. Purified water and ethyl alcohol of various concentrations (20%, 40%, 70%, 90%) were used as the extractant. Next, carried out the selection of the optimal conditions for the complexation reaction (the complexation reaction time, the ratio of «aliquot – aluminum chloride alcohol solution»). The method was validated according to GPM.1.1.0012.15 of the State Pharmacopoeia (SF) XIII edition and generally accepted methods for the following indicators: specificity, analytical field, linearity, accuracy, precision.Results and discussion. The optimal parameters for extracting flavonoids from raw materials were determined (threefold extraction with ethyl alcohol 70% in a water bath, the ratio of «raw material – extractant» – 1:30 for 30 minutes, the particle size of the raw material – 2.0 mm). The conditions for the complexation reaction were selected (the ratio «aliquot: aluminum chloride alcohol solution» – 1: 2.5, the complexing agent – aluminum chloride solution 5% alcohol, the appearance of a stable solution color after 40 minutes). When carrying out the validation of the developed method, it was established that the validation characteristics under study are within the acceptance criteria. When analyzing the raw materials harvested in the Altai Territory in different years, it was found that the content of flavonoids in common sorrel herba ranges from 0,596 to 0,632%.Conclusion. The optimal parameters of extraction of flavonoids from raw materials were determined, the conditions for the complexation reaction were selected, and the developed method was validated. The quantitative content of flavonoids in terms of rutin in sorrel sour grass harvested in the Altai Territory in different years has been established.

scholarly journals QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE FRUITS OF CHINESE LYCIUM CHINENSE MILL.

2020 ◽  
pp. 139-144
Author(s):  
Yevgeniy Yevgen'yevich Kurdyukov ◽  
Yelena Fedorovna Semenova ◽  
Inessa Yakovlevna Moiseyeva ◽  
Natal'ya Aleksandrovna Gavrilova ◽  
Tat'yana Andreyevna Ponomareva
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
Raw Material ◽  
Lycium Chinense ◽  
Optimal Conditions ◽  
Single Determination ◽  
Layer Chromatography ◽  
Β Carotene ◽  
Acetone Hexane

The objects of the study were Mature dried fruits of Chinese birch (Lycium chinense Mill., this. Solanaceae (Solanaceae)). Four samples were examined: No. 1 (Gifts of Pamir, Russia), No. 2 (Gullin Tianhe Pharmaceutical, China), No. 3 (A. J. Alliance, Russia), No. 4 (Globaltorg, Russia). The purpose of this work is to determine the amount of carotenoids in the fruits of Chinese by spectrophotometry. The quantitative determination of the amount of carotenoids in the raw material of Chinese Dereza was carried out. Thin-layer chromatography was used to confirm the presence of carotenoids in the fruits of Dereza. The expediency of using the spectrophotometric method for the detection and quantitative determination of carotenoids in extracts from the fruits of Chinese birch is substantiated. The electron spectra of hexane, acetone and acetone-hexane solutions in the wavelength range 400–500 nm have a maximum optical density at 450±2 nm, characteristic of β-carotene. The optimal conditions of extraction of carotenoids from the raw materials of this plant (extractant – hexane; ratio "raw material–extractant" – 1 : 5; extraction time – 90 minutes; the degree of grinding of raw materials – 0.5 mm). It was determined that the error of a single determination of the content of carotenoids in the fruits of Chinese Dereza with a confidence probability of 95% is ±3,49%. It was revealed that the content of carotenoids in the raw material of Chinese Dereza varies in the range of 33–39 mg%.

New colorimetric method for quantitative determination of protein in urine.

1977 ◽  
Vol 23 (5) ◽  
pp. 811-812 ◽  
Author(s):  
H Yatzidis
Keyword(s):  
Quantitative Determination ◽  
Tannic Acid ◽  
Chloride Solution ◽  
Room Temperature ◽  
Colorimetric Method ◽  
Ferric Chloride Solution ◽  
Acid Protein ◽  
Analytical Recovery ◽  
Violet Color

Abstract Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected. The CV and analytical recovery ranged from 0.5 to 1.8% and 98 to 103%, respectively. Nonprotein urinary constituents do not interfere.

Sign in / Sign up

Export Citation Format

Share Document